This document discusses transcription and gene expression. It begins by explaining that transcription occurs from 5' to 3' as RNA polymerase adds nucleotides to the growing mRNA molecule. It then provides understandings about transcription in eukaryotes including that nucleosomes help regulate it, mRNA is modified after transcription through splicing and addition of a cap and tail. Finally, it explains that gene expression is regulated through factors that bind DNA and affect transcription as well as through DNA methylation and modification of chromatin structure.