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7.2 Transcription and Gene
Expression
Guidance
RNA polymerase adds the 5’ end of the free RNA nucleotide to the 3’ end of the
growing mRNA molecule
Understandings
- Transcription occurs in a 5’ to 3’ direction
- Nucleosomes help to regulate
transcription in eukaryotes
- Eukaryotic cells modify mRNA after
transcription
- Splicing of mRNA increases the number
of different proteins an organism can
produce
- Gene expression is regulated by proteins
that bind to specific base sequences in
DNA
- The environment of a cell and of an
organism has an impact on gene
expression
Applications/Skills
- A: the promoter as an example of
non-coding DNA with a function
- S: Analysis of changes in the DNA
methylation patterns
http://dnamismatch.com/Test/wp-content/uploads/2013/07/Sense-and-antisense.gif
Transcription: Initiation
• RNA polymerase binds to the DNA at the promoter and breaks the
hydrogen bonds between the nitrogenous bases, separating the
strands
• A transcription bubble opens
Helicase
Transcription: Elongation
• RNA nucleotides are added
(5’3’ direction)
http://geneed.nlm.nih.gov/images/transcription_sm.jpg
Transcription: Termination
• Terminator signals for the
release of RNA
polymerase
• Transcription stops
• RNA detaches
• This is the primary mRNA
transcript
mRNA Modification
• Primary transcript has introns
removed (splicing)
• Exons are put together to form
mature mRNA
• These can be arranged in multiple
orders, increasing the number of
possible proteins
• 5’ cap and poly-A tail are added to
protect the mRNA in the cytoplasm
http://upload.wikimedia.org/wikipedia/commons/a/a6/Rna_splicing.JPG
Control of gene expression
• Addition of methyl groups to
segments of DNA/chromosomes
• Transcription factors to help bind
RNA polymerase
• Transcription activators that loop
DNA between the activator and
promoter
• Repressor proteins that prevent
transcription of a segment of DNA
http://mcubed.umich.edu/sites/default/files/projects/Slide1_6.jpg
DNA Methylation
http://xarquon.jcu.cz/edu/zbb/prednasky/09nucleus/092function/images/activation3.jpg
Epigenetics
http://www.frontiersin.org/files/Articles/105911/fcell-02-00049-HTML/image_m/fcell-02-00049-g001.jpg

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7.2 transcription

  • 1. 7.2 Transcription and Gene Expression
  • 2. Guidance RNA polymerase adds the 5’ end of the free RNA nucleotide to the 3’ end of the growing mRNA molecule Understandings - Transcription occurs in a 5’ to 3’ direction - Nucleosomes help to regulate transcription in eukaryotes - Eukaryotic cells modify mRNA after transcription - Splicing of mRNA increases the number of different proteins an organism can produce - Gene expression is regulated by proteins that bind to specific base sequences in DNA - The environment of a cell and of an organism has an impact on gene expression Applications/Skills - A: the promoter as an example of non-coding DNA with a function - S: Analysis of changes in the DNA methylation patterns
  • 4. Transcription: Initiation • RNA polymerase binds to the DNA at the promoter and breaks the hydrogen bonds between the nitrogenous bases, separating the strands • A transcription bubble opens Helicase
  • 5. Transcription: Elongation • RNA nucleotides are added (5’3’ direction) http://geneed.nlm.nih.gov/images/transcription_sm.jpg
  • 6. Transcription: Termination • Terminator signals for the release of RNA polymerase • Transcription stops • RNA detaches • This is the primary mRNA transcript
  • 7. mRNA Modification • Primary transcript has introns removed (splicing) • Exons are put together to form mature mRNA • These can be arranged in multiple orders, increasing the number of possible proteins • 5’ cap and poly-A tail are added to protect the mRNA in the cytoplasm http://upload.wikimedia.org/wikipedia/commons/a/a6/Rna_splicing.JPG
  • 8. Control of gene expression • Addition of methyl groups to segments of DNA/chromosomes • Transcription factors to help bind RNA polymerase • Transcription activators that loop DNA between the activator and promoter • Repressor proteins that prevent transcription of a segment of DNA http://mcubed.umich.edu/sites/default/files/projects/Slide1_6.jpg DNA Methylation http://xarquon.jcu.cz/edu/zbb/prednasky/09nucleus/092function/images/activation3.jpg