The document outlines the practical applications of immunology, focusing on vaccines and diagnostic immunology. It discusses the history and principles of vaccination, describing different types of vaccines such as live attenuated, inactivated, toxoid, subunit, and conjugated vaccines. It also covers the development of new vaccines and safety testing. For diagnostic immunology, it explains immunologic diagnostic tests and specific techniques such as monoclonal antibodies, precipitation reactions, agglutination reactions, neutralization reactions, fluorescent-antibody techniques, and enzyme-linked immunosorbent assays (ELISA). These diagnostic methods detect antibodies or antigens in patient samples.
Applications of immunological functions(Sp13-bty-001) CIIT Abbottabad Zohaib HUSSAIN
Applications of Immunological functions
Introduction
Immunological function is define as biological effects of immune system on antigens during immune responds
Effects may be
1.Pathological effects (By keeping normal homeostasis)
2.Physiological effects (Resulting in diseases)
Main functions of immunity
1.Defense
2.Homeostasis
3.Surveillance
Physiological and pathological representation of immune response
Function Physiological
(advantageous) Pathological
(harmful)
1.Immune defense Resist to pathogen Immunologic deficiency disease
2.Immune homeostasis scavenge damaged Autoalergic disease
3.Immune surveillance Scavenge cells with misreplication Cell cancerization persistent infection
Applications of Immunological functions
1.Treatment (Vaccines)
Immunity to a disease is achieved through the presence of antibodies to that disease in a person system. Antibodies are proteins produced by the body to neutralize or destroy toxins or disease-carrying organisms. Antibodies are disease specific. For example, measles antibody will protect a person who is exposed to measles disease, but will have no effect if he or she is exposed to mumps.
There are two types of immunity: active and passive.
a)Active Immunity
It results when exposure to a disease organism triggers the immune system to produce antibodies to that disease. Exposure to the disease organism can occur through infection with the actual disease (natural immunity), or introduction of a killed or weakened form of the disease organism through vaccination (vaccine-induced immunity). If an immune person comes into contact with that disease in the future, their immune system will recognize it and immediately produce the antibodies needed to fight it. Active immunity is long-lasting, and sometimes life-long.Vaccines are available for all of the following vaccine-preventable diseases Typhoid hepatitis A and B influenza and many more
b)Passive Immunity
It is provided when a person is given antibodies to a disease rather than producing them through his or her own immune system. A newborn baby acquires passive immunity from its mother through the placenta. A major advantage of passive immunity is protection is mediated whereas active immunity takes time usually 2 to 3 weeks but it is for only few weeks for months only active immunity is long lasting e.g. tetanus Pertussis Diphtheria etc. is cure by passive immunity
2.Diagnosis
It use purified antibody solutions (antiserum) to diagnose disease. Diagnostic antibodies can be produced to detect particular microbe
A. In animals (mixed antiserum)
Inject animal with microbe or antigenic fragments
Allow immune response (1-2 weeks)
Harvest blood
Purify antibodies from serum to make antiserum = purified antibody solution to one particular antigen. These preparations will produce multiple Antibody types that recognize
ANTIGEN, HAPTEN, ALL TYPES OF ANTIGENS, IMMUNOGEN , ATTRIBUTES OF ANTIGENICITY, DETERMINANTS OF ANTIGENICITY,
IMMUNOLOGY KUBY, MEDICAL MICROBIOLOGY & IMMUNOLOGY OF PANIKER , LIPPINCOTT'S IMMUNOLOGY, OTHER SOURCES.
Applications of immunological functions(Sp13-bty-001) CIIT Abbottabad Zohaib HUSSAIN
Applications of Immunological functions
Introduction
Immunological function is define as biological effects of immune system on antigens during immune responds
Effects may be
1.Pathological effects (By keeping normal homeostasis)
2.Physiological effects (Resulting in diseases)
Main functions of immunity
1.Defense
2.Homeostasis
3.Surveillance
Physiological and pathological representation of immune response
Function Physiological
(advantageous) Pathological
(harmful)
1.Immune defense Resist to pathogen Immunologic deficiency disease
2.Immune homeostasis scavenge damaged Autoalergic disease
3.Immune surveillance Scavenge cells with misreplication Cell cancerization persistent infection
Applications of Immunological functions
1.Treatment (Vaccines)
Immunity to a disease is achieved through the presence of antibodies to that disease in a person system. Antibodies are proteins produced by the body to neutralize or destroy toxins or disease-carrying organisms. Antibodies are disease specific. For example, measles antibody will protect a person who is exposed to measles disease, but will have no effect if he or she is exposed to mumps.
There are two types of immunity: active and passive.
a)Active Immunity
It results when exposure to a disease organism triggers the immune system to produce antibodies to that disease. Exposure to the disease organism can occur through infection with the actual disease (natural immunity), or introduction of a killed or weakened form of the disease organism through vaccination (vaccine-induced immunity). If an immune person comes into contact with that disease in the future, their immune system will recognize it and immediately produce the antibodies needed to fight it. Active immunity is long-lasting, and sometimes life-long.Vaccines are available for all of the following vaccine-preventable diseases Typhoid hepatitis A and B influenza and many more
b)Passive Immunity
It is provided when a person is given antibodies to a disease rather than producing them through his or her own immune system. A newborn baby acquires passive immunity from its mother through the placenta. A major advantage of passive immunity is protection is mediated whereas active immunity takes time usually 2 to 3 weeks but it is for only few weeks for months only active immunity is long lasting e.g. tetanus Pertussis Diphtheria etc. is cure by passive immunity
2.Diagnosis
It use purified antibody solutions (antiserum) to diagnose disease. Diagnostic antibodies can be produced to detect particular microbe
A. In animals (mixed antiserum)
Inject animal with microbe or antigenic fragments
Allow immune response (1-2 weeks)
Harvest blood
Purify antibodies from serum to make antiserum = purified antibody solution to one particular antigen. These preparations will produce multiple Antibody types that recognize
ANTIGEN, HAPTEN, ALL TYPES OF ANTIGENS, IMMUNOGEN , ATTRIBUTES OF ANTIGENICITY, DETERMINANTS OF ANTIGENICITY,
IMMUNOLOGY KUBY, MEDICAL MICROBIOLOGY & IMMUNOLOGY OF PANIKER , LIPPINCOTT'S IMMUNOLOGY, OTHER SOURCES.
The content is about Immunology for to recall the topic " Antigen presentation and processing" - specifically about the type two pathway "Exogenous antigen presentation and processing". I just presented the content to the best of my knowledge with the reference of two books - Kuby immunology and Janeway's immunobiology. I hope this was helpful to the life science aspirants and other students in life science sector.
T cells are one of the important white blood cells of the immune system and play a central role in the adaptive immune response and are distinguished from other lymphocytes by the presence of a T-cell receptor (TCR) on their cell surface.
B cells, also known as B lymphocytes, are a type of white blood cell of the lymphocyte subtype. They function in the humoral immunity component of the adaptive immune system.. B cells produce antibody molecules.
In mammals, B cells mature in the bone marrow, which is at the core of most bones. In birds, B cells mature in the bursa of Fabricus.
B cells present antigens (they are also classified as professional antigen-presenting cells (APCs)) and secrete cytokines.
The content is about Immunology for to recall the topic " Antigen presentation and processing" - specifically about the type two pathway "Exogenous antigen presentation and processing". I just presented the content to the best of my knowledge with the reference of two books - Kuby immunology and Janeway's immunobiology. I hope this was helpful to the life science aspirants and other students in life science sector.
T cells are one of the important white blood cells of the immune system and play a central role in the adaptive immune response and are distinguished from other lymphocytes by the presence of a T-cell receptor (TCR) on their cell surface.
B cells, also known as B lymphocytes, are a type of white blood cell of the lymphocyte subtype. They function in the humoral immunity component of the adaptive immune system.. B cells produce antibody molecules.
In mammals, B cells mature in the bone marrow, which is at the core of most bones. In birds, B cells mature in the bursa of Fabricus.
B cells present antigens (they are also classified as professional antigen-presenting cells (APCs)) and secrete cytokines.
Radioimmunoassay (RIA) is a very sensitive in vitro assay technique used to measure concentrations of antigens (for example, hormone levels in blood) by use of antibodies. As such, it can be seen as the inverse of a radiobinding assay, which quantifies an antibody by use of corresponding antigens.
this is a series of notes on clinical pathology, useful for undergraduate and post graduate pathology students. Notes have been prepared from standard textbooks and are in a format easy to reproduce in exams.
A vaccine is a biological agent that provides active acquired immunity to a particular disease. A vaccine usually contains an agent that resembles a disease-causing microorganism. It is often made from killed or weakened forms of the microbe, its toxins or one of its surface proteins. Body's immune system is stimulated to recognize the agent as a threat and destroy it, and any of these microorganisms that it later encounters.
Antibiotics are used against a wide range of pathogens and are very important in preventing and treating infections. The use of appropriate choice of antibiotics, dose and enforcing compliance is important in patient's care and preventing drug resistance.
Update on Vaccine Issues & WSAVA Guidelines (2015-2017)Yotam Copelovitz
Dr. Jean Dodds opens her presentation with an explanation on how animals obtain immunity. She continues it by discussing some key points on vaccine issues and their relationship to memory cell immunity. The presentation is concluded with suggested alternatives to current vaccine practices, such as titer testing.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
2. Outline
I. VACCINES
I. History
II. Principles and Effects of Vaccination
III. Types of Vaccines
IV. Development of New Vaccines
V. Safety of Vaccines
II. DIAGNOSTIC IMMUNOLOGY
I. Immunologic-Based Diagnostic Test
II. Monoclonal Antibodies
III. Precipitation Reactions
IV. Agglutination Reactions
V. Neutralization Reactions
VI. Complement-Fixation Reaction
VII. Fluorescent-Antibody Techniques
VIII. Enzyme-Linked Immunosorbent Assay (ELISA)
3. VACCINES
• suspension of organisms or
part of organisms used to
induce immunity
•Immune response mimics
recovery from disease
9. TYPES OF VACCCINES
•LIVE ATTENUATED VACCINES
•Closely resemble actual infection
•Typical lifelong immunity
•Problem: live microbe can
mutate to virulent form
23. Monoclonal Antibodies
• Technique to make a single type of
antibody in unlimited amounts
• Fusion of an antibody producing B cell
with a myeloma cell – immortal antibody
producing cell
• HYBRIDOMA
• Hybridoma cells produce large quantities
of a specific antibody
24. Monoclonal Antibodies
Uses and Problems
• Uses
• Diagnostic
• Pregnancy tests
• Therapeutic
• Transplant rejection
• Cancer treatment – Herceptin
• Problems
• Antibodies are mouse protein and cause
reactions in the human body
26. Precipitin Reactions
• Reaction of soluble antigens with IgG
and IgM antibodies
• Form visible molecular aggregates
called LATTICES
• Precipitation only occurs where the
ratio of antigen to antibody is optimal
34. Neutralization Reactions
• Antigen-antibody reaction that can
block the harmful effects of toxins or
viruses
• Neutralizing substance is an antibody
produced by the host
• Called an ANTITOXIN
• Antitoxin combines with the toxin and
the toxin is neutralized
35.
36. Fluorescent-Antibody Techniques
• used to identify microorganisms in
clinical specimens or antibodies in
serum
• Combine antibodies with a
fluorescent dye
• Direct FA tests
•Microorganisms in clinical samples
• Indirect FA tests
•Detect antibodies in serum
37.
38. Enzyme –linked immunosorbent
assay (ELISA)
• Antigen- antibody reactions are detected
by enzyme activity.
• If the indicator enzyme is present in the
test well, an antigen- antibody reaction
has occurred.
39. Enzyme –linked immunosorbent
assay (ELISA)
• Direct ELISA
• Detects antigen
• Common use is in drug screening in
urine
• Indirect ELISA
• Detects antibodies in patient sample
• Common example is screening blood for
antibodies to HIV