For each step of DNA replication, predict the outcome if : • concentration of nitrogenous bases is low • primase is inhibited • DNA polymerase were inhibited • DNA ligase were inhibited • Helicase were inhibited Solution All the above situations lead to inhibition of DNA replication. Please find the below flow-chart regarding DNA replication for the better understanding. DNA Replication process Double-stranded DNA Helicase Separation of 2 strands Single stranded binding (SSB) proteins Binds with the separated strands and keeps them from reannealing Primase Primase creates the primer (DNA Polymerase starts working from primers) on both strands One strand acts as a Leading strand and other one is Lagging strand DNA Polymerase Addition of new DNA bases on the separated strands in the direction of 5’ to 3’ Ligase Fill the gaps between Okazaki fragments formed on Lagging strand Formation of two separate double-stranded DNAs.

1. For each step of DNA replication, predict the outcome if
: • concentration of nitrogenous bases is low
• primase is inhibited •
DNA polymerase were inhibited •
DNA ligase were inhibited • Helicase were inhibited
Solution
All the above situations lead to inhibition of DNA replication. Please find the below flow-chart
regarding DNA replication for the better understanding.
DNA Replication process
Double-stranded DNA
Helicase
Separation of 2 strands
Single stranded binding (SSB) proteins
Binds with the separated strands and keeps them from reannealing
Primase
Primase creates the primer (DNA Polymerase starts working from primers) on both strands
One strand acts as a Leading strand and other one is Lagging strand
DNA Polymerase
Addition of new DNA bases on the separated strands in the direction of 5’ to 3’
Ligase
Fill the gaps between Okazaki fragments formed on Lagging strand
Formation of two separate double-stranded DNAs