rna splicing is a process of removal of introns from rna molucle

1. Gov. science
college
Biotech
sem3
Khushal
khambhati
Roll no. 18

2. • the removal of introns from a primary
transcript and the subsequent joining
of exons in the production of a
mature RNA molecule.
RNA
splicing
transcriptio
n
exons
intron
s
Rna
splicing
translation
DNA PRE-
MRNA
MATURED
RNA Polypeptid
e

3. history
phillip Allen Sharp is an American
geneticist and molecular biologist
who co-discovered RNA splicing. He
shared the 1993 Nobel Prize with
Richard J. Roberts for "the discovery
that genes in eukaryotes are not
contiguous strings but contain
introns, and that the splicing of
messenger RNA to delete those
introns can occur in different ways,
yielding different proteins from the
same DNA sequence".
phillip Allen Sharp Richard J. Roberts

4. 1 2 4 5 6 8 9 103 7
1 2 5 7 6 8 9 104 51 4 6 4

5. 1 2 4 5 6 8 9 103 7
1 4 52 1 4 6 7 64 8 9 105
1 2 5 7 6 8 9 104 51 4 6 4
1 4 52 1 4 6 7 64 8 9 105

6. Splicing
site
GU site
Branch site
Pyridines
track
Splicing
site
AG site

7. BREAKING
PHOSPHODIESTE
R BOND DUE TO
ADDITION OF –OH
GROUP
FORMATION OF
PHOSPHODIESTE
R BOND –
formation of loop

8. but what
would
help to do
these
Spliceoso
me

9. • It is a large and complex
molecular machine which
helps to remove the introns
from pre-Mrna molecule
Spliceosome
●The complexes of snRNA and
proteins are called small nuclear
ribonuclear proteins (snRNP,
pronounces “snurps”).
There are 5 snrnp’s
U1, U2, U4, U5, U6
● Spliceosome act by formation
of-
COMPLEX E
COMPLEX A
COMPLEX B
COMPLEX C

10. u1
u2
u4
u5
u6

11. E complex
a complex
a complex
b complex
b complex
c complex
u1 bbp
u2af65
u2af36
u1
u1
u1
u1
bbp
u1
u2
u2
u2af65
u2af36
u2
U6, u4, u5
u4
U6, u4, u5
u2

12. Rna splicing
Introduction
Why intron
are
important
process Spliceosome Complex

13. Reference
 http://genetics.thetech.org/ask/ask326
 http://www2.oakland.edu/biology/chaudhry/pics/chapter24f.pdf
 http://www.dnalc.org/resources/animations/rna-splicing.html
 http://highered.mheducation.com/olcweb/cgi/pluginpop.cgi?it=swf::535::535::/site
s/dl/free/0072437316/120077/bio30.swf::How+Spliceosomes+Process+RNA
 http://www.evolutionnews.org/2013/09/the_spliceosome_1076371.html
 http://www.evolutionnews.org/2011/12/finding_treasur054241.html