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GKM
Ahmed
Splicing
EI
GKM
Splicing
Splicing
Remove of Intron and joining the exon together
Three Classes of RNA Splicing
Nuclear pre-mRNA
Group I introns
Group II introns
EIE
GKM
Splicing
exon 3’
Group II introns
Intron5’ exon GU5’ 3’AG
OH
P
AOH
Splice Site Splice Site
branch site
Transesterification
which phosphodiester linkages within the
pre-mRNA are broken and new ones are formed
2’
Nucleophile
intron lariat
GKM
Splicing
exon 3’
Group I introns
5’ exon GU5’ 3’AG
OH
P
GOH
Splice Site Splice Site
3’
GKM
Splicing
Spliceosome
150 Proteins 5 RNAs
U1, U2, U4, U5, U6
100 -300
snRNP
Small Nuclear Ribonuclear Proteins
GKM
Splicing
exon 3’
Spliceosome
5’ exon GU5’ 3’AG
AOH
3’ Splice Site 5’ Splice Site
branch site
2’
U2U1 U2AFBBp
Branch point
Binding Protein
U4
U6 U5
GKM
Splicing
exon 3’
Spliceosome
5’ exon
AOH 2’
U2U4
U6 U5
exon 3’
AOH 2’
U2
U5
GKM
Splicing
5’ exon
U6
exon 3’
OH 2’
U2
U5
Spliceosome
A
OH
Intron lariat

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Splicing