Splicing , Spliceosome , Remove Introns
Remove of Intron and joining the exon together
Three Classes of RNA Splicing
Group I introns
Group II introns
Nuclear pre-mRNA
3. GKM
Splicing
exon 3’
Group II introns
Intron5’ exon GU5’ 3’AG
OH
P
AOH
Splice Site Splice Site
branch site
Transesterification
which phosphodiester linkages within the
pre-mRNA are broken and new ones are formed
2’
Nucleophile
intron lariat