The document discusses GKM cloning vectors, which are small DNA pieces allowing the insertion of foreign DNA into host cells. It outlines the types of cloning vectors, including plasmids, bacteriophages, and cosmids, emphasizing their essential features such as small size, genetic mapping, and the presence of multiple cloning sites. Additionally, it touches on challenges related to replication, expression, and potential issues with translation and transcription when foreign DNA is inserted.

1. GKM
Cloning Vector
Ahmed

2. What we do
If we want to insert a foreign DNA inside a host ,it does not contain this piece
?Because DNA not contain origin of replication (oriC)
# If foreign DNA Combined with the host chromosome?
Detection Expression
In Host
Position?
Fail
Translation
Transcription
GKM
Cloning Vector
Replicon

3. Cloning Vector
is a small piece of DNA Allow the foreign DNA inserted inside cell
GKM
Cloning Vector
Types of cloning vectors.
• Plasmid
• Bacteriophage
• Cosmid
• BAC
• YAC
• HAC Features of a cloning vector
Small Size Genetic map sequence
Fast Replication
Reporter gene
2 Reporter gene Insertional
inactivation
Multiple Cloning Site
(MCS)