The document discusses RNA synthesis and mRNA processing in eukaryotes. It describes that mRNA processing begins with transcription by RNA polymerase, which then adds a 5' methylated cap. Specific nucleotide sequences in the mRNA are bound by cleavage factors, which leads the 3' end to configure for cleavage. Stabilizing factors are added and poly-A polymerase synthesizes an adenine tail by polyadenylation at the 3' end, completing mRNA processing.

1. RNA
(RNA STRUCTURE, RNA SYNTHESIS, MRNA PROCESSING IN
EUKARYOTES)
Cherry Anne C. Mendoza BS BIO-II

17. RNA SYNTHESIS

31. MRNA PROCESSING
IN EUKARYOTES

37. Processing of mRNA begins with transcription

38. Soon after, RNA Polymerase begins transcription

39. A Methylated Cap is added to 5’ end. Transcriptuon continues.

40. Following completion, RNA Polymerase releases rhe cap strand of pre-mRNA

41. Specific nucleotide sequence in the mRNA are bound by cleavage factors.

42. The 3’ end of the mRNA next move into the correct configuration of cleavage.

43. Stabilizing Factors are then added to the complex.

44. Poly A Polymerase now binds to the mRNA and cleaves the 3’end.

45. The complex begins to dissociate and the ckeaved 3’ end qu8ckly degrades.

46. Poly A Polymerase now synthesizes the polyadebylated taol by adding adenine
residues to the cleavage site.

47. Additional proteins then bind to the tail increasing the rate at which it grows.

48. When the tail reaches its full lenght, the Poly A Polymerase is signaled to
stop.

49. Adding residue and the polyadenylation process is completed. The processed
mRNA is now ready to undergo splicing in preparation for translation.