Gel electrophoresis
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Is a technique used to separate and sometimes purify macro molecules ,especially protein and nucleic acid.
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Gel electrophoresis
- 2. GKM Gel Electrophoresis Is a technique used to separate and sometimes purify macromolecules ,especially protein and nucleic acid. Gel Electrophoresis PCR CloningVector + Gene Vector Gene 10 kbp 5 kbp 10 kbp 5 kbp 15 kbp
- 3. GKM Gel Electrophoresis Purpose Gel Electrophoresis Separating DNA How it SeparatingPorous Based on Shape Charge Size Smaller size 100 bp Moved faster than Larder size 500 bp N.B S p Nicked Linear Supercoiled Nicked Linear Supercoiled
- 4. GKM Gel Electrophoresis Type of Gel Polyacrylamide Gel Agarose Gel Polysaccharide polymers extracted from seaweed (good separation for large5–10kbp DNA fragments) (good resolution for small 0.2–1kbp fragments) 0.8% 2 %
- 5. GKM Gel Electrophoresis Steps Agarose 0.8 % 100ml TBE boiling Ethidium bromide60 c ladder
- 6. GKM Gel Electrophoresis Gel Electrophoresis under Ultraviolet Ladder Exon 4,5 PTEN