This course introduces students to the basics and intricacies of Medical Microbiology. The students will learn about the history, relationship between hosts and microbes, and safety measures in a Clinical Microbiology lab in the first unit. The second unit imparts knowledge about the various types of microscopes, the principles of sterilization, and biomedical waste management. The final unit delves into the characteristics, growth, and nutrition of microbes, the methods of culture, an introduction to Immunology, and the care and handling of laboratory animals. The course is designed to provide a comprehensive understanding of these topics, supplemented with practical lab experience to foster a solid foundation for future explorations in the field of Medical Microbiology. Created by: Mr. Attuluri Vamsi Kumar, Assistant Professor, Department of MLT, UIAHS, Chandigarh University, Mohali, Punjab. For more details website: https://www.mltmaster.com

1. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Program Name: B.Sc. Medical Lab Technology
Syllabus of General Clinical Microbiology
Course Name: General Clinical Microbiology Course Type
Course Coordinator: Attuluri Vamsi Kumar – B.Sc. MLT, M.Sc. MLT, PhD (Perusing)
Designation: Assistant Professor
Department: Department of Medical Lab Technology (MLT)
Program Core
PRE-REQUISITE Introduction to National Healthcare Delivery
System in India, Medical Terminology and
record keeping, Introduction to Quality and
Patient safety
Sem: 2 S. No: 11
About Vamsi: I am academician in Medical Laboratory Sciences with a strong desire to improve Outcome
based education (OBE) structured MLT education. I am constantly focusing on building an academic
atmosphere that is set high standards with strong multi blended teaching pedagogy models.
YouTube: https://www.youtube.com/@vamsiMLT
SlideShare: https://www.slideshare.net/VamsiIntellectual
Website: www.mltmaster.com / https://sites.google.com/view/vamsi-intellectual-protfolio/home
LinkedIn: https://www.linkedin.com/in/vamsi-kumar-attuluri-ab8987128/?originalSubdomain=in
Research Gate: https://www.researchgate.net/profile/Attuluri-Kumar
Orcid ID: https://orcid.org/0000-0001-9278-6714
Contact No: +91 7416660584
Mail ID: vamsifmlt@gmail.com
A. COURSE DESCRIPTION
This course introduces students to the basics and intricacies of Medical Microbiology. The
students will learn about the history, relationship between hosts and microbes, and safety
measures in a Clinical Microbiology lab in the first unit. The second unit imparts knowledge
about the various types of microscopes, the principles of sterilization, and biomedical waste
management. The final unit delves into the characteristics, growth, and nutrition of microbes,
the methods of culture, an introduction to Immunology, and the care and handling of laboratory
animals. The course is designed to provide a comprehensive understanding of these topics,
supplemented with practical lab experience to foster a solid foundation for future explorations
in the field of Medical Microbiology.
B. COURSE OBJECTIVES:
1. To recall the history of Medical Microbiology, the definition of key terminologies, and the
properties of various microbes.
2. To comprehend the principles behind different types of microscopy, sterilization methods, and the
growth and nutritional requirements of microbes.
3. To apply the learned knowledge in practice, such as the care and handling of glassware and
laboratory equipment, the use of microscopes, and the preparation and use of culture media.
4. To analyze different types of microbial cultures, the working of sterilizers and their efficiency, and
the effect of different nutritional and physical conditions on bacterial growth.
5. To evaluate the importance of safety measures in Clinical Microbiology, the effectiveness of
different sterilization and disinfection methods, and the ethical and legal aspects of handling
laboratory animals.
C. COURSE OUTCOMES
CO
No
Statement Performance
Indicator
Level of
Learning
Target
Attainment

2. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
(Highest BT
Level)
CO1 Students will be able to recall and explain the
fundamental concepts, history, and terminology of
Medical Microbiology, along with the
characteristics and classification of different
microbes.
PI 6 K1 70%
CO2 Students will comprehend and elucidate the
operation mechanism of different types of
microscopes, sterilization methods, growth, and
nutritional requirements of microbes.
PI 2.5, PI 6 K2 70%
CO3 Students will apply their understanding to perform
laboratory tasks effectively, such as care and
handling of glassware and equipment, preparation
and use of culture media, and working with different
types of microbial cultures.
PI 2.1, PI 2.2,
PI 2.4, PI 2.5,
PI 7
K3 70%
CO4 Students will analyze and interpret different
sterilization methods, microbial cultures, the effect
of different nutritional and physical conditions on
bacterial growth, and the efficiency of different
disinfection methods.
PI 2.5, PI 3, PI
4
K4 70%
CO5 Students will be able to design and conduct
experiments using different sterilization methods,
create different types of microbial cultures, and
manage biomedical waste effectively.
PI 2.5, PI 5, PI
6
K5 70%
CO6 Students will critically evaluate the importance of
safety measures in Clinical Microbiology, the
effectiveness of different sterilization and
disinfection methods, and the ethical and legal
aspects of handling laboratory animals.
PI 2.2, PI 2.3,
PI 4, PI 9, PI
10
K6 70%
D. SYLLABUS
Unit-1 Introduction to Medical Microbiology & Laboratory
Equipment
Contact Hours:15
Chapter 1.1 Understanding Medical Microbiology- Definition, History, Host - Microbe relationship,
Safety measures in Clinical Microbiology
Chapter 1.2 Working with Laboratory Equipment- Glassware used in Clinical Microbiology Laboratory:
Introduction, Care and handling of glassware, Cleaning of glassware, Equipment used in clinical
Microbiology Laboratory: Introduction, Care and maintenance including calibration
Experiment 1 /
Case study
To demonstrate safe code of practice for a Microbiology laboratory
Experiment 2 /
Case study
To prepare cleaning agents & to study the technique for cleaning & sterilization of glassware
Experiment 3 /
Case study
To demonstrate the working & handling of Compound microscope
Unit-2 Microscopy, Sterilization & Waste Management Contact Hours:15
Chapter 2.1 Exploring the Microscopic World - 11
Chapter 2.2 Sterilization and Biomedical Waste Management- Definition, Types and principles of
sterilization methods: Heat (dry heat, moist heat with special Reference to autoclave),
Radiation, Filtration, Efficiency testing to various sterilizers, Antiseptics and disinfectants:
Definition, Types and properties, Mode of action, Precautions while using the disinfectants,
Qualities of a good disinfectant, Testing efficiency of various disinfectants, Biomedical waste
management in a Medical Microbiology laboratory

3. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Experiment 4 /
Case study
4.1 To demonstrate the method of sterilization by autoclave including its efficacy testing
4.2To demonstrate the method of sterilization by hot air oven including its efficacy testing
4.3 To demonstrate the method of sterilization of media/solution by fil
Experiment 5 /
Case study
5.1 Demonstration of Antiseptics, Spirit, Cetrimide & Povidone-Iodine
5.2 To demonstrate the use of disinfectants
5.3 Demonstrate the precaution while using disinfectants
Experiment 6 /
Case study
6. To prepare working dilution of commonly used disinfectants
Experiment 7 /
Case study
7.1 Rideal-walker phenol co-efficient test
7.2 Kelsey-Sykes test
Unit-3 Microbes and their Growth, Culture, and Handling Contact Hours:15
Chapter 3.1 Understanding Microbes and their Growth- General characteristics & classification of
Microbes: (Bacteria & fungi), Growth and Nutrition of Microbes: General nutritional & other
requirements of the bacteria, Classification of bacteria on the basis of their nutritional
requirements, Physical conditions required for growth, Normal growth cycle of bacteria
(growth curve), Types of microbial cultures
Chapter 3.2 Culture Methods and Introduction to Immunology- Culture media: Introduction,
Classification of culture media, Quality control in culture media, Automation in culture media
preparation, Aerobic & anaerobic culture methods: Concepts, Methods used, Introduction to
Immunology: Immunity, Antigens and Antibodies, Care & handling of laboratory animals:
Introduction, General care & handling, Ethics & legality in use of laboratory animals
Experiment 8 /
Case study
8.1 To demonstrate the different morphological types of bacteria
8.2 Preparation of one culture media from each type
Experiment 9 /
Case study
To demonstrate aerobic culture
Experiment 10 /
Case study
10.1 To demonstrate anaerobic culture
10.2 Visit to animal house & demonstrate about care of laboratory animals
Self-study topics for Advance learners: genetic variation in microbes, gene transfer methods,
improving antimicrobial use, including the selection, dose, route of administration, and duration of
antimicrobial therapy.common infectious diseases, including their epidemiology, microbial
characteristics, and public health challenges.
E. TEXT BOOKS/REFERENCE BOOKS
TEXT BOOKS
T1 "Medical Microbiology" by Patrick R. Murray, Ken S. Rosenthal, and Michael A. Pfaller:
T2 "Clinical Microbiology Made Ridiculously Simple" by Mark Gladwin and Bill Trattler
T3 "Microbiology: A Laboratory Manual" by Cappuccino and Sherman
REFERENCE BOOKS
R1 "Medical Microbiology & Immunology: Examination & Board Review" by Warren
Levinson
R2 "Jawetz Melnick & Adelbergs Medical Microbiology" by Stefan Riede
R3 "Koneman's Color Atlas and Textbook of Diagnostic Microbiology" by Gary W. Procop
and Elmer W. Koneman

4. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Code Performance Indicators
PI 1 Demonstrate professional interpersonal, oral, and written communications skills sufficient to
serve the needs of patients and the public including an awareness of how diversity may affect
the communication process.
PI 2 Perform pre-analytical, analytical, and post-analytical processes:
PI 2.1 Demonstrate ability to understand investigation/test requisition.
PI 2.2 Collecting the relevant clinical samples along with complete and accurate documentation with
proper safety measures in relation to sample accountability.
PI 2.3 To transport the samples with precautionary measures to the relevant lab section.
PI 2.4 Demonstrate the ability to prepare clinical sample for processing
PI 2.5 To demonstrate the knowledge of accurate sample processing for the required lab
investigation. Perform routine clinical laboratory tests in clinical chemistry,
haematology/haemostasis, immunology, immunohematology, microbiology, Histopathology,
Cytopathology, body fluid analysis, and laboratory operations.
PI 2.6 Perform mathematical calculations related to all areas of the clinical laboratory
PI 2.7 Ability to record the test results/data.
PI 2.8 To demonstrate the ability to interpret the test reports and its documentation in lab records.
PI 2.9 Demonstrate ability to release the report to the right person in minimum turn-around time
(TAT).
PI 3 Perform problem solving and troubleshooting techniques for laboratory methodologies
Correlate laboratory test results with patient diagnosis and treatment.
PI 4 To follow basic quality assessment protocol of clinical laboratory.
PI 5 Demonstrate routine laboratory techniques sufficient to orient new employees within the
clinical laboratory.
PI 6 Apply basic scientific principles in learning new techniques/procedures; demonstrate
application of principles and methodologies.
PI 7 Utilize computer technology applications to interact with computerized instruments and
laboratory information systems.
PI 8 Demonstrate adequate knowledge of computer software as it applies to document production,
spreadsheets, and presentations.
PI 9 Demonstrate professional behaviour with co-team mates.
PI 10 Demonstrate sensitivity and compassion towards patients.