This course provides students with a comprehensive understanding of systematic bacteriology, including bacterial culture techniques, staining procedures, biochemical tests, and laboratory diagnostics. The course focuses on providing both theoretical knowledge and practical experience to prepare students for a career in the field. The content is divided into three main units: Basic Bacteriology and Staining Techniques, Biochemical Tests for Bacteria Identification and Characteristic Identification, and Advanced Bacteriology and Lab Diagnostics. Created by: Mr. Attuluri Vamsi Kumar, Assistant Professor, Department of MLT, UIAHS, Chandigarh University, Mohali, Punjab. For more details website: https://www.mltmaster.com

1. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Program Name: B.Sc. Medical Lab Technology
Syllabus of Systematic Bacteriology
Course Name: Systematic Bacteriology Course Type
Course Coordinator: Attuluri Vamsi Kumar – B.Sc. MLT, M.Sc. MLT, PhD (Perusing)
Designation: Assistant Professor
Department: Department of Medical Lab Technology (MLT)
Program Core
PRE-REQUISITE General Clinical Microbiology Sem: 3 S. No: 19
About Vamsi: I am academician in Medical Laboratory Sciences with a strong desire to improve Outcome
based education (OBE) structured MLT education. I am constantly focusing on building an academic
atmosphere that is set high standards with strong multi blended teaching pedagogy models.
YouTube: https://www.youtube.com/@vamsiMLT
SlideShare: https://www.slideshare.net/VamsiIntellectual
Website: www.mltmaster.com / https://sites.google.com/view/vamsi-intellectual-protfolio/home
LinkedIn: https://www.linkedin.com/in/vamsi-kumar-attuluri-ab8987128/?originalSubdomain=in
Research Gate: https://www.researchgate.net/profile/Attuluri-Kumar
Orcid ID: https://orcid.org/0000-0001-9278-6714
Contact No: +91 7416660584
Mail ID: vamsifmlt@gmail.com
A. COURSE DESCRIPTION
This course provides students with a comprehensive understanding of systematic bacteriology,
including bacterial culture techniques, staining procedures, biochemical tests, and laboratory
diagnostics. The course focuses on providing both theoretical knowledge and practical
experience to prepare students for a career in the field. The content is divided into three main
units: Basic Bacteriology and Staining Techniques, Biochemical Tests for Bacteria
Identification and Characteristic Identification, and Advanced Bacteriology and Lab
Diagnostics.
B. COURSE OBJECTIVES:
1. List and describe the instruments used in bacterial culture and staining techniques.
2. Explain the principles, procedures, and interpretations of different biochemical tests for bacterial
identification.
3. Perform a variety of bacterial culture procedures and staining techniques in a laboratory setting.
4. Analyze the results of biochemical tests and staining procedures to identify different bacteria.
5. Evaluate the characteristics, pathogenesis, and laboratory diagnosis procedures of various bacteria.
C. COURSE OUTCOMES
CO
No
Statement Performance
Indicator
Level of
Learning
(Highest BT
Level)
Target
Attainment
CO1 The student will be able to recall the fundamental
principles of bacterial culture, staining techniques,
and biochemical tests used for the identification of
bacteria.
PI 2.5, PI 6 K1:
Remembering
70%
CO2 The student will be able to explain the
morphological, cultural, and biochemical
characteristics of various bacteria, and interpret the
results of staining procedures and biochemical tests.
PI 2.8, PI 3 K2:
Understanding
70%

2. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
CO3 The student will be able to apply bacterial culture
procedures, perform staining techniques, and carry
out various biochemical tests in a laboratory setting.
PI 2.1, PI 2.4,
PI 2.5, PI 5
K3: Applying 70%
CO4 The student will be able to analyze the results of
biochemical tests and staining procedures to
identify and classify different bacteria, and
understand their pathogenesis.
PI 2.8, PI 3 K4: Analyzing 70%
CO5 The student will be able to evaluate and assess the
effectiveness of different bacterial identification
techniques, including culture methods, staining, and
biochemical tests.
PI 2.9, PI 4 K5: Evaluating 70%
CO6 The student will be able to develop efficient
strategies for bacterial identification and diagnosis,
taking into consideration the morphology, cultural
characteristics, and biochemical properties of
various bacteria.
PI 2.7, PI 3, PI
4
K6: Creating 70%
D. SYLLABUS
Unit-1 Basic Bacteriology and Staining Techniques Contact Hours:15
Chapter 1.1 Bacterial Culture- Instruments used to seed culture media, Culture procedures – seeding a
plate
Chapter 1.2 Staining Techniques in Bacteriology- Significance of staining in bacteriology
Principle, Reagent preparation, procedures and interpretation of the following:
Simple staining
Negative staining
Gram stain
Albert‘s stain
Neisser‘s stain
Ziehl –Neelsen staining
Experiment 1 /
Case study
Demonstrate the instruments used to seed culture media.
Experiment 2 /
Case study
Learn techniques for Inoculation of bacteria on culture media.
Experiment 3 /
Case study
Isolate specific bacteria from a mixture of organisms.
Unit-2 Biochemical Tests for Bacteria Identification and
Characteristic Identification
Contact Hours:15
Chapter 2.1 Biochemical Tests for Identification of Bacteria - 19
Chapter 2.2 Characteristic Identification of Bacteria- Definition, Classification, Various characteristics
(morphological, cultural and biochemical), pathogenesis and laboratory diagnosis of the
following bacteria: Staphylococcus, Streptococcus, Pneumococcus, Neisseria gonorrhea and
Neisseria meningitis, Haemophilis, Corynebacterium, Enterobacteriaceae (including
Escherichia coli, Klebsiella, Citrobacter, Enterobacter, Proteus, Salmonella, Shigella, Yersinia
enterocolitica and Yersinia pestis), Vibrio, Aeromones and Plesiomonas, Clostridia of wound
infection.
Experiment 4 /
Case study
Demonstrate simple staining (Methylene blue)
Experiment 5 /
Case study
Prepare India ink preparation to demonstrate negative staining.
Experiment 6 /
Case study
Demonstrate reagent preparation, procedure and interpretation for Gram stain, Albert stain,
Neisser‘s staining, Z-N staining, Capsule staining, Demonstration of flagella by staining
methods, Spore staining, and demonstrate spirochetes by Fontana staining

3. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Experiment 7 /
Case study
Prepare the reagent and demonstrate following biochemical tests with positive and negative
control bacteria: Catalase, Coagulase, Indole, Methyl Red (MR), VogesProskauer (VP),
Urease, Citrate, Oxidase, TSIA, Nitrate reduction, Carbohydrate fermentation, H
Unit-3 Advanced Bacteriology and Lab Diagnostics Contact Hours:15
Chapter 3.1 Advanced Bacterial Characteristics and Diagnostics- Definition, Classification, Various
characteristics (morphological, cultural and biochemical), pathogenesis and laboratory
diagnosis of the following bacteria: Mycobacterium tuberculosis complex, Atypical
Mycobacteria and M. leprae, Spirochetes – Treponema, Borrellia and leptospira, Bordetella
and brucella, Mycoplasma and Ureaplasma, Rickettsia, Chlamydia, Actinomyces,
Pseudomonas and Burkholderia, Brief introduction about non-sporing anaerobic cocci and
bacilli.
Chapter 3.2 Practical Laboratory Techniques in Systematic Bacteriology- Techniques for the
application of staining methods, biochemical tests, and bacterial characteristic identification in
a laboratory setting.
Experiment 8 /
Case study
To demonstrate various characteristics (morphological, cultural and biochemical) of bacteria
commonly isolated from clinical samples i.e. Staphylococcus, Streptococcus,
Corynebacterium, Escherichia coli, Klebsiella, Citrobacter, Enterobacter, Proteus, Sal
Experiment 9 /
Case study
To conduct laboratory diagnosis of specific bacteria, implementing culture, staining, and
biochemical testing methods.
Experiment 10 /
Case study
To apply the learning of the course in practical scenarios involving bacterial identification and
diagnostics.
Self-study topics for Advance learners: Advanced Bacterial Genetics: This topic will deal with
the understanding of bacterial chromosome structure, plasmids, transposons, bacteriophages, genetic
recombination, mutation, and repair mechanisms.Bacterial Pathogenesis and Host Interaction: This
can cover topics like molecular mechanisms of bacterial pathogenesis, including adherence, invasion,
evasion of host defenses, toxin production, and other virulence factors. It could also include an
understanding of the host immune response to bacterial infections.
E. TEXT BOOKS/REFERENCE BOOKS
TEXT BOOKS
T1 "Brock Biology of Microorganisms" by Madigan, Michael T., Kelly S. Bender, Daniel H.
Buckley, W. Matthew Sattley, David A. Stahl
T2 "Microbiology: An Introduction" by Tortora, Funke, and Case
T3 "Microbiology: A Systems Approach" by Marjorie Kelly Cowan
REFERENCE BOOKS
R1 "Medical Microbiology" by Murray, Rosenthal, Pfaller
R2 "Clinical Microbiology Made Ridiculously Simple" by Mark Gladwin, Bill Trattler,
C.Scott Mahan
R3 "Jawetz Melnick & Adelbergs Medical Microbiology" by Brooks, Carroll, Butel, Morse

4. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Code Performance Indicators
PI 1 Demonstrate professional interpersonal, oral, and written communications skills sufficient to
serve the needs of patients and the public including an awareness of how diversity may affect
the communication process.
PI 2 Perform pre-analytical, analytical, and post-analytical processes:
PI 2.1 Demonstrate ability to understand investigation/test requisition.
PI 2.2 Collecting the relevant clinical samples along with complete and accurate documentation with
proper safety measures in relation to sample accountability.
PI 2.3 To transport the samples with precautionary measures to the relevant lab section.
PI 2.4 Demonstrate the ability to prepare clinical sample for processing
PI 2.5 To demonstrate the knowledge of accurate sample processing for the required lab
investigation. Perform routine clinical laboratory tests in clinical chemistry,
haematology/haemostasis, immunology, immunohematology, microbiology, Histopathology,
Cytopathology, body fluid analysis, and laboratory operations.
PI 2.6 Perform mathematical calculations related to all areas of the clinical laboratory
PI 2.7 Ability to record the test results/data.
PI 2.8 To demonstrate the ability to interpret the test reports and its documentation in lab records.
PI 2.9 Demonstrate ability to release the report to the right person in minimum turn-around time
(TAT).
PI 3 Perform problem solving and troubleshooting techniques for laboratory methodologies
Correlate laboratory test results with patient diagnosis and treatment.
PI 4 To follow basic quality assessment protocol of clinical laboratory.
PI 5 Demonstrate routine laboratory techniques sufficient to orient new employees within the
clinical laboratory.
PI 6 Apply basic scientific principles in learning new techniques/procedures; demonstrate
application of principles and methodologies.
PI 7 Utilize computer technology applications to interact with computerized instruments and
laboratory information systems.
PI 8 Demonstrate adequate knowledge of computer software as it applies to document production,
spreadsheets, and presentations.
PI 9 Demonstrate professional behaviour with co-team mates.
PI 10 Demonstrate sensitivity and compassion towards patients.