1. CURRICULUM –VITAE
K.HARI BABU
M,SC : M.Phil Email:hari_micro4@yahoo.co.in
Eye Bank Manager - SANKARA NETHRALAYA - CHENNAI , T.N, INDIA.
(Ex.AssistantMicrobiologist/Research Associate-LV.P.E.I)
(Ex. Microbiologist & Eye Bank Manager )
Sankara Eye Hospital Phone:09940472042/09959386102
Guntur – Vijayawada Express High Way. 9866074029.
Pedakakani, Guntur – A.P, INDIA.
OBJECTIVE:
To gain adequate skills in Microbiological Sciences and a challenging position in the
field of Biological Sciences with an eye towards new Technologies. To work in an
organization of a growth-oriented environment which provides unlimited scope for the
projects of challenging nature. My communication skills, positive attitude, smart work
and commitment towards work will prove to be valuable asset to your esteemed
organization
EDUCATIONALQUALIFICATIONS:
• M.Phil : ( Medical Microbiology – on “Diagnosis of Microbial keratitis (Non viral )
by Conventional Microbiological Methods and Pilot Study of PAN Fungal PCR in
the Diagnosis of Fungal keratitis” ” ) from L.V. Prasad Eye and Research Institute,
Hyderabad. Under the registration of Nagarjuna University , Andhra Pradesh - INDIA.
• M.Sc : (Microbiology) from Bharathidasan University,Tiruchirapally Tamil Nadu.
• B.Sc : (Botany,Zoology,Chemistry) from Nagarjuna university.

2. PresentAssignment:
Working as an “Eye Bank Manager” in Sankara Nethralaya, Chennai from 26th
October 2015 to till date.
Last Experience in Pharma company:
Worked as “ Senior Analyst “ for MEGSAN Labs Pvt.Ltd ( CRO – Contract Research
Organization) – Kompally - Hyderabad, Telanga, India from May 2nd 2014 to October
17th 2015.
JOB RESPONSIBILITIES IN MEGSAN LABS:
Regular maintenance of test reports, log books, and raw data sheets in
Microbiology lab.
Coordinate Annual Trend Reports for Water and Environmental Monitoring data.
Oversee the Calibration and Preventive Maintenance Programmed.
Follow up of routine/non-routine quality monitoring of Utility systems inclusive
of Purified water.
Maintenance of the routine/non-routine environmental monitoring for all required
plants, facilities, clean rooms, hygienic areas and labs, inclusive of particle
monitoring test.
Perform Media receive, sterility check and Growth promotion tests for media used
for testing.
Perform qualification and validation of equipment.
PROFESSIONAL CAPABILITIES:
Water testing.
Microbial limit test (MLT).
Environmental Monitoring performing.
Growth promotion testing for the media.
Validation of Media Shelf Life.
Validation of Disinfectant study.
Calibrations of Various Equipment.
Media Preparation.
Handling of Cultures from Different passages.
Culture maintences.
Fumigation and DE fumigation processes.

3. Operation of Double Door Autoclave.
Performance verification of Various Equipment.
MIC, AET.
Instruments Handled and well aware ofall the Performance
verifications
Double Door Autoclave.
Incubator.
Hot Air Oven.
Laminar flow hood
pH meter, weighing balance.
Air sampler.
EXPERIENCE IN MEDICAL SECTOR/OPTHALMOLOGY:
Worked as a “ Microbiologist & Eye Bank Manager ” in Sankara Eye Hospital ,
Guntur A.P. INDIA. ( A Unit of Sankara Eye Care Institutions - Coimbatore - Tamil
Nadu, INDIA. (from April 5th 2010 to July 2013.)
PREVIOUS EXPERIENCE
1) Established Eye Bank and Microbiology Departments in Sankara Eye Hospital -
Guntur, Andrapradesh, India. In the year 2010 April 1st and became country head
for entire Sankara Eye Care Institution - Coimbatore - Tami Nadu, INDIA.
Job description in Sankara Eye Hospital - Guntur - A.P, India
1) Marketing of Eye Banking in 2- 3 districts in and around Guntur ( Lions clubs,
Universities, colleges, old age homes, schools, banks, etc ) for the promotion of Corneal
blindness and Eye Donation importance in public.
2) From Tissue, harvesting to till the tissue goes to operation theater, entire processing ,
documentation, monitoring of eye bank.
3) Daily examination of Microbiology scraping slides, culture identification, Antibiotic
susceptibility test.
4) Enclosing the Interim and Final reports in the case sheets of Microbiology reports.

4. MOST VALID EXPERIENCESIN L.V.PRASAD EYE INSTITUTE
1. Worked as a “ Research Assistant “ in Jhaveri Microbiology Center, Hyderabad Eye
Research Foundation . L.V. Prasad Eye Institute from 2/06/2004 to 13/12/2006. in
various Research Projects.
2.Worked as an “Assistant Microbiologist” in L.V. Prasad Eye and Research
Institute. Hyderabad, A.P. INDIA.(from 14th December 2006 to 16th January , 2010 )
Teaching Experience:
1. 4 years experience in taking classes of Microbiology for the B,SC Optometry
students in Bausch & Lomb school (A Unit of L.V.P.E.I)
2. Examiner for the final semester students of optometry.
3. Valuation of the B,SC Optometry students Microbiology papers

5. RESEARCH EXPERIENCE IN LV PRASAD EYE INSTITUTE
1) Worked as a Research Assistant / Research Associate for “Antibacterial activity
of contact lens study” an Australian (Sydney - CCLRU ( CIBA VISION) funded
research project in L.V. Prasad Eye and Research Institute – Hyderabad.
ABCL STUDY:
Aim: To determine the incidence of serious significant and non significant adverse
events if any, with contra lateral wear of FOCUS NIGHT & DAY (FND)
antibacterial lenses ( test) versus FND lenses (control) when worn on a 30 night
( 30N) EW and disposal schedule.
1. Processing of the sample (Lens and lid swabs)
2. Identification of the grown organism.( Using Mini API )
3. Stocking the organisms.
4. Adhesion study. ( 3 days Experiment)
In this study, we will put the lenses (which are antibacterial coated with silver and
other type of lenses are F&D lenses) in the patients eye, and collect it after every 1week,
1month, 3months & 6months.after these periods lenses will be collected in sterile PBS,
and lenses will be inoculated in chocolate agar plates by using sand witch method, and
the lens solution will be inoculated in 3 different ways like aerobically, anaerobically and
for fungal too. After 48 hours of incubation these plates, aerobic plates will be taken out
and using Mini API will do further processing. system. And anaerobic plates will be
taken out from incubators after 4 days, and finally for fungal plates which are inoculated
in sabouraud’s dextrose agar, reading will be taken after 7 days of incubation.
And very important experiment in this study was the adhesion study. In this
experiment, some of the lenses which are randomized according to the patient diagnosis,
will be collected and infect the lenses with the P.seudomonas aeruginosa.
Staphyolococcus.epeidermidis. andcertain dilutions will be done and reading will be
taken. Iam not going to details of the procedures because the work was confidential.

6. 2) Worked as a Research Assistant for “Clinical efficacy & safety of T 1225 1.4%Eye
drops ( 3day treatment ) versus Tobramycin 0.3% Eye drops ( 7day treatment ) in
the treatment of Purulent Bacterial Conjunctivitis” a France country( THEA
LABORATORIES) funded Research Project in R&D in L.V Prasad Eye and Research
Institute- Hyderabad.
In this project we came to know that bacterial conjunctivitis will be
caused not only by major normal flora like Staphylococcus, Streptococcus, we found
came to know that some, other bacteria like E.coli, Klebsiella etc .which are Gram
negative bacteria also cause bacterial conjunctivitis. As project is confidential am not
going to details of the methodology.
3) Worked as a Research Assistant for “Pilot determination of the antimicrobial
efficacy of 10 foaming hand cleansers using the health care personnel hand wash
procedure.” Standard Test Method Evaluation of the Effectiveness of Health care
personnel consumer hand wash formulations. ASTM method E:174-00. Standard test
methods for evaluation of in activators of Antimicrobial agents. E:1054-02.
Purpose of the Study.
1) The purpose of the study is to determine the antimicrobial effectiveness of three
Antibacterial Foam cleansers for use as Health care Personnel Hand washes. The
indicator microorganism used for hand wash contaminations will be Serratia marcescens
(ATCC # 14756) Eight subjects will testes per test article for a total of twenty –four
subjects. Over the course of eleven consecutive hand contamination. The first followed
by a sample for baseline, and the remaining ten by product applications, with microbial
samples taken after product applications one, three, seven and ten.
2. Determine the effect of surfactant load on the performance of foaming cleanser
prototypes.
3.Accrue additional benchmark data for Antibacterial Foam Cleanser prototype 3.

7. TECHNIQUES KNOWN:
1. PCR -RT-PCR, Multiplex& PAN fungal PCR,Viral PCR for HSV,VZV,CMV..
2. Electrophoretic Techniques : PAGE & AGAROSE..
3. Hybridization Techniques : Southern, Nothern & Western Hybridizations.
4 .Isolation techniques- Plasmid DNA isolation chromosomal DNA isolation.
5. Bacteriological, Mycology, Serological investigations by using various
automations and Manual methods.
6. Isolation of Bacteria, Fungi, Protozoa.
7. Detection of HSV by using IFA Test.
8. Staining Techniques.
9. Identification of specific pathogens by Biochemical tests.
10. Slide Agglutination and precipitation.
11. Eyrthrocyte Sedimentation rate.
12. Total and differential count of blood cells.
13. Experience in handling Bio-Merieux Vitek systems vidas, Bacteria Alert 3D &
classical systems of Blood and TB cultures.
14. Experience in handling of specimen in clinical microbiology , mycology,
serology, Parasitological, aerobic & anaerobic bacteriology, Sterility checking of
operation theaters & Bacteriology of water and evaluation of the efficiency of
disinfectants.

8. SEMINARS AND WORKSHOPS PARTICIPATED:
1. Participated in a National seminar at Nagarjuna University on “Modern Biology”
conducted by DST and ICMR, New Delhi from 28th to 30th August 2003.
2. Attended 14th& 15th Annual Meeting of Indian Eye Research group ( 30,&31st of
July 2005 to 2008) conducted in L.V. Prasad Eye and Research Institute
Hyd.A.P.INDIA.
PRESENTATIONS:
1. Presented a paper and given a lecture on “Antibacterial activity of few natural
compounds extracted from nine plants”, in a national symposium on
“Regenerative medicine and Tissue culture engineering” organized by Nagarjuna
University on 28th and 29th April 2004.
2 Given Poster Presentation on “ Ocular Infections Caused by “Non fermentative
Gram Negative Bacilli other than Pseudomonas aeruginosa ''and their Susceptibility
to Commonly used antibiotics.”in Asia ARVO 2009 ( Association of Research in Vision
and Opthalmology ) International conference conducted in Hi tex Hyderabad January 10 -
14th 2009.
K. Hari Babu1, G.Ashok kumar Reddy1, Usha Gopinathan1, Prashant Garg2, Subhdra
Jalali3.
3. Given Poster Presentation in All Indian Association of medical Microbiologists
meet in Mysore October 2008 on “ Susceptibility Trends of Streptococcus
pneumoniae isolated from ocular infections to commonly used Antibiotics “
G. Ashok kumar Reddy1, K.HariBabu2Usha Gopinathan1, Prashant Garg2, Subhdra
Jalali3.

9. Jhaveri Microbiology Centre, Prof Brien Holden Eye Research Center, Hyderabad Eye
Research Foundation,
Hyderabad, India
2Cornea and Anterior Segment Services, L.V. Prasad Eye Institute, Hyderabad, India
PUBLICATIONS
“In Vitro Antibiotic susceptibility test of Rapidly Growing Non tuberculous
Mycobacteria isolated from Patients with Microbial Keratitis” Received 29 April
2009; revised 11 November 2009;accepted 13 November 2009
G. Ashok kumar Reddy1, K. Hari Babu1, Prashant Garg2,Usha Gopinathan1, and
Savitri Sharma1, in “Current Eye ResearchResearch“ ( American Journal)
1Jhaveri Microbiology Centre, Prof Brien Holden Eye Research Center, Hyderabad Eye
Research Foundation,
Hyderabad, India
2Cornea and Anterior Segment Services, L.V. Prasad Eye Institute, Hyderabad, India
REFERENCES

10. 1. Dr. Savitri Sharma,
Head, Laboratory Services of Entire L.V.P.E.I Networks , INDIA.
Ex.Editor Indian Journal of Medical Microbiology,
Ex. President, Indian Association of Medical Microbiologist.
Patia, Bhubhaneshwar –751021,INDIA
Cell No : 09489995521
2. Dr. P. Sudhakar
M,S : FLVPEI,FMRF
Chief Medical Officer, Sankara Eye Hospital - Guntur, A. P, INDIA
( A Unit of Sankara Eye Care Institution- Coimbatore- Tamil Nadu, INDIA)
Cell; 09951604138: 0863- 2594100
3. Prof. M.Vijaya Lakshmi
Head, Dept.of Botany & Microbiology.
Chairperson Board of studies, Microbiology,
Nagarjuna University, Guntur, A.P,INDIA
Cell: 09440870026
4. Dr. Pravin Krishna
M,S ( Ophthalmology)
Cornea Consultant
L.V. Prasad Eye Institute – Hyderabad
Cell : 0849094245
Personal Profile:

11. Father’s Details: K. Koteswar Rao
Rtd. S.I of Police.
Age & Sex 30,Male
Languages known: English, Hindi, Telugu and Tamil.
Permanent Address: 407, NDR Estates,
Kailash Nagar colony,
Chandanagar (P.O),
Lingampally
HYDERABAD-58, INDIA.
Declaration :
I here by declare that all the above mentioned information is true and
correct to the best of my knowledge. I also assure that I”11 perform well if I were given
an opportunity.
Yours sincerely
( Hari Babu.K)