This document discusses pureline selection, which is a plant breeding method where a single, homozygous and self-pollinated plant is selected and its progeny evaluated. In pureline selection, a large number of plants from a self-pollinated crop are individually selected and harvested, and the best individual plant progeny is released as a pureline variety. All plants within a pureline have an identical genotype. The document outlines the characteristics, uses, applications, advantages and disadvantages of pureline selection as a plant breeding technique.

1. Sher-e-Kashmir
University of Agricultural Sciences
and Technology of Kashmir
Shalimar Campus
Majid Rashid

2. PURELINE SELECTION
• INTRODUCTION
• A Pureline is the progeny of a single ,
homozygous , self-pollinated plant.
• In Pureline selection a large number of plants are
selected from a self pollinated crop and
harvested individually; individual plant progenies
from them are evaluated and the best progeny is
released as a Pureline variety
• All the individuals within a Pureline have identical
genotype and any variation present within a
Pureline is solely due to environment.

3. Characteristics of Purelines
• All the plants within a Pureline have the same
genotype as the plant from which the Pureline
was derived since the parent plant was
homozygous and self-fertilized.
• Variation present within a Pureline is due to
the environment and non heritable.
• Purelines become genetically variable with
time due to mechanical mixture ,natural
hybridization and mutations.

4. Uses of Purelines
• As a variety
• As Parents in hybridization Programme
• In studies on mutation

5. Applications of Pureline Selection
• Improvement of Local varieties.
• Selection in Introduced varieties.
• Improvement of old Pureline varieties.
• Selection for a New characteristic in a
Pureline.

6. Advantages of Pureline Selection
• It achieves the maximum possible
improvement over the original variety.
• Pureline varieties are extremely uniform; they
are therefore preferred by farmers and
consumers to a less uniform variety developed
through Mass selection
• Due to extreme uniformity, the variety is easily
identified in seed certification programmes.

7. Disadvantages of Pureline Selection
• The varieties developed through Pureline
selection generally do not have the wide
adaptation and stability in the production
possessed by the local varieties from which they
are developed.
• Pureline selection requires more time, space and
expensive yield trials than Mass selection.
• The improvement is dependent on the genetic
variation present in the original population .
• The breeder has to devote more time to Pureline
Selection than to Mass selection.

8. First year 200-3000 plants are selected for
Desi or old variety superior phenotype
Second l l l l l l l l l l l Individual Plant progenies are grown
year Individual Plant progenies Undesirable progenies are rejected
Third Selected Progenies are planted in preliminary
Year Preliminary yield trial yield trial & inferior ones are rejected
Fourth to Multi location yield trials with suitable checks
sixth year are conducted. Disease resistance and quality
Multi location yield trials tests are done.
Seventh Progeny superior to check variety is released as a new
year variety. Seed multiplication begins for distribution
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