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SAM HIGGIN BOTTOM UNIVERSITY OF AGRICULTURE,
TECHNOLOGY & SCIENCES, NAINI, ALLAHABAD
DEPARTMENT OF PLANT BREEDING AND GENETICS
TOPIC: PURE LINE THEORY AND PURE LINE SELECTION
PRESENTED BY
P. NIKHIL
19MSCGPB177
SUBMITTED TO:
Dr.B.G.SURESH
DEPT OF GENETICS AND PLANT BREEDING
• Started experiments in 1901.Established 19 pure lines by selection of
individual beans from the mixed seed lot of 8kg.(named those as A,B,...,T)
• Followed selection among these purelines too and selected the smallest and
largest beans (seeds).planted those selected seeds.
Pure LineTheory
Johannsen developed concept of pure line while working with princess bean (
Phaseolus vulgaris nana).Princess bean exhibits self -pollination
Procedure:
Johannsen’s theory and conclusions:
• Continuous inbreeding (selfing) leads to homozygosity.
• Variation with in a pure line results from environment only.
• Selection with in pure line is not effective because all the plants in a pure line have
some genotype.
• Selection in the original population is effective because the plants have genetic
variation.
PURE LINE SELECTION
INTRODUCTION:
• A pure line is the progeny of a single homozygous, self pollinated plants.
• In pre line selection a large number of plants are selected from a self
pollinated crop and harvested individually; individual plants progeny from
them are evaluated and the best progeny is released as a pure line variety.
• All the individuals within a pure line have identical genotype and any
variation present within a pure line solely due to environment.
Characteristics of Purelines:
• All the plants within a Pureline have the same genotype as the
plant from which the pureline was derived since the parent plant
was homozygous and self-fertilized.
• Variation present within a pureline is due to the environment and
non heritable.
• Purelines become genetically variable with time due to
mechanical mixture, natural hybridization and mutations.
Uses of Purelines
• As a variety
• As Parents in hybridization programme.
• In studies on mutation
• Pure lines are used for studying mutations and other biological investigations
such as medicine, immunology, physiology, and biochemistry.
Application of Pureline selection
• Improvement of local varieties.
• Selection in introduced varieties.
• Improvement of old Pureline varieties.
• Selection for a new characteristic in a pureline.
Pure line selection Procedure:
Advantages of Pureline Selection
• It achieves the maximum possible improvement over the original variety.
• Pureline varieties are extremely uniform; they are therefore preferred by
farmers and consumers to a less uniform variety developed through mass
selection.
• Due to extreme uniformity, the variety is easily identified in seed
certification programmes.
Disadvantages of Pureline Selection
• The varieties developed through Pureline selection generally do not have the wide
adaptation and stability in the production possessed by the local varieties from
which they are developed.
• Pureline selection requires more time, space and expensive yield trials than mass
selection.
• The improvement is dependent on the genetic variation present in the original
population.
• The breeder has to devote more time to pureline selection than to mass selection.
THANKYOU

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Pure Line Theory and Pure line Selection

  • 1. SAM HIGGIN BOTTOM UNIVERSITY OF AGRICULTURE, TECHNOLOGY & SCIENCES, NAINI, ALLAHABAD DEPARTMENT OF PLANT BREEDING AND GENETICS TOPIC: PURE LINE THEORY AND PURE LINE SELECTION PRESENTED BY P. NIKHIL 19MSCGPB177 SUBMITTED TO: Dr.B.G.SURESH DEPT OF GENETICS AND PLANT BREEDING
  • 2. • Started experiments in 1901.Established 19 pure lines by selection of individual beans from the mixed seed lot of 8kg.(named those as A,B,...,T) • Followed selection among these purelines too and selected the smallest and largest beans (seeds).planted those selected seeds. Pure LineTheory Johannsen developed concept of pure line while working with princess bean ( Phaseolus vulgaris nana).Princess bean exhibits self -pollination Procedure:
  • 3. Johannsen’s theory and conclusions: • Continuous inbreeding (selfing) leads to homozygosity. • Variation with in a pure line results from environment only. • Selection with in pure line is not effective because all the plants in a pure line have some genotype. • Selection in the original population is effective because the plants have genetic variation.
  • 4. PURE LINE SELECTION INTRODUCTION: • A pure line is the progeny of a single homozygous, self pollinated plants. • In pre line selection a large number of plants are selected from a self pollinated crop and harvested individually; individual plants progeny from them are evaluated and the best progeny is released as a pure line variety. • All the individuals within a pure line have identical genotype and any variation present within a pure line solely due to environment.
  • 5. Characteristics of Purelines: • All the plants within a Pureline have the same genotype as the plant from which the pureline was derived since the parent plant was homozygous and self-fertilized. • Variation present within a pureline is due to the environment and non heritable. • Purelines become genetically variable with time due to mechanical mixture, natural hybridization and mutations.
  • 6. Uses of Purelines • As a variety • As Parents in hybridization programme. • In studies on mutation • Pure lines are used for studying mutations and other biological investigations such as medicine, immunology, physiology, and biochemistry.
  • 7. Application of Pureline selection • Improvement of local varieties. • Selection in introduced varieties. • Improvement of old Pureline varieties. • Selection for a new characteristic in a pureline.
  • 8. Pure line selection Procedure:
  • 9. Advantages of Pureline Selection • It achieves the maximum possible improvement over the original variety. • Pureline varieties are extremely uniform; they are therefore preferred by farmers and consumers to a less uniform variety developed through mass selection. • Due to extreme uniformity, the variety is easily identified in seed certification programmes.
  • 10. Disadvantages of Pureline Selection • The varieties developed through Pureline selection generally do not have the wide adaptation and stability in the production possessed by the local varieties from which they are developed. • Pureline selection requires more time, space and expensive yield trials than mass selection. • The improvement is dependent on the genetic variation present in the original population. • The breeder has to devote more time to pureline selection than to mass selection.