Allen and Arnon medium produces higher cyanobacteria concentration compared to BG-11 medium. (Berberoglu et al., 2008) Macroalgae culture requires higher concentration of vitamins than microalgae cultures. Microalgae cultured on different medium shows no morphological differences. Artificial and enriched medium have higher concentration than marine environment. Ammonium concentration higher than 25 µM is toxic to some species. Medium composition may affect cellular composition specifically lutein and fatty acid (eicosapentaenoic acid) (Seto et al., 1984; Miron et al., 2008)

1. Effect of Culture Medium
for
Marine Microalgae
Vijendren s/o Krishnan
Supervisor: Prof. Dr. Uemura
(13 Feb 2014)

2. Background studies
• Allen and Arnon medium produces higher
cyanobacteria concentration compared to BG-11
medium. (Berberoglu et al., 2008)
• Macroalgae culture requires higher concentration
of vitamins than microalgae cultures.
• Microalgae cultured on different medium shows
no morphological differences.

3. • Artificial and enriched medium have higher
concentration than marine environment.
• Ammonium concentration higher than 25 μM is
toxic to some species.
• Medium composition may affect cellular
composition specifically lutein and fatty acid
(eicosapentaenoic acid) (Seto et al., 1984; Miron
et al., 2008)

4. Component
Stock solution
(g/L) Quantity
Final concentration
(g/L)
Macronutrient
NaNO3 15 5 0.075
NaH2PO4H2O 5 1 5x10-3
Na2SiO39H2O 30 1 0.03
Micronutrient
FeCl36H20 3.15
Na2EDTA2H2O 4.36
CuSO45H2O 9.8 1 9.8x10-3
Na2MoO42H2O 6.3 1 6.3x10-3
ZnSO47H2O 22 1 0.022
CoCl26H2O 10 1 0.01
MnCl24H2O 180 1 0.18
Vitamins
Thiamine HCl (B1) 0.2g
Biotin (H) 1 1 1x10-3
Cyanocobalamin (B12) 1 1
1x10-3
F2 Medium

5. Mann and Myers Mann and Myers, 1968
Composition Stock concentration
(g/L)
Quantity (ml) Final concentration
(g/L)
MgSO47H2O 120 10 1.2
NaNO3 100 10 1
CaCl2 30 10 0.3
K2HPO4 10 10 0.1
Na2EDTA 3 10 0.03
H3BO3 0.6 10 6x10-3
FeSO47H2O 0.2 10 2x10-3
MnCl2 0.14 10 1.4x10-3
ZnSO47H2O 0.033 10 3.3x10-4
Co(NO3)26H2O 0.014 0.5 7x10-6
CuSO45H2O 0.004 0.5 2x10-6

6. Allen and Arnon Allen and Arnon, 1955
Component Stock solution (g/L) Quantity
(ml/L)
Final concentration
(g/L)
+Pi K2HPO43H2O
K2HPO4 anhydrous
70
53.5
5 0.35
0.2675
-Pi 1. MgSO4 (MgSO47H20) 100 10 1
2. CaCl2 (CaCl22H20) 30 10 0.3
3. NaCl 100 10 1
4. Microelement (Add in order) Stock solution (mg/L) 10
Distilled water 850 ml - -
A&A Fe-EDTA* 400 ml - 10.96 + 16.32
MnCl4HO 900 - 7.2x10-3
22MoOMolybdic acid (85%)
90
- 7.2x10-4
3 NaMoO2H0 (99%)
152.75
1.22x10-3
242ZnSO47H2O 110 - 8.8x10-4
CuSO45H2O 39.5 - 3.16x10-4
H3BO3 1430 - 0.01144
NH4VO3 (NH4+ metavanadate) 11.5 - 9.2x10-5
CoCl26H2O 20 - 1.6x10-4

7. Artificial Sea Water
SALT Final Concentration G/L
NaCl 24.6
KCl 0.67
CaCl22H2O 1.36
MgSO47H2O 6.29
MgCl26H2O 4.66
NaHCO3 0.18

8. Composition of various medium
Medium F2 A&A M & M ASW
Cation
Ca+2 - 0.082 0.108 0.37
Co+2 2.353x10-3 3.966x10-4 1.42x10-6 -
Cu+2 2.512x10-3 8.04x10-5 5.086x10-7 -
Fe+3 0.653 1.937 4.029x10-4 -
K+ - 2.66 0.0449 0.351
Mg+2 - 0.097 0.118 1.18
Mn+2 0.05 2x10-3 6.111x10-4
Na+ 0.5672 2.16232 0.2747 9.72
NH+ - 1.415x10-5 - -
4
SO4
-2 0.011 3.71 0.4678 2.4496
Zn+2 5x10-3 2x10-4 7.51x10-5
Anion
BO3
-2 - 0.0108 5.709x10-3
Cl- 1.31 0.7546 0.6404 17.5334
CO3
-2 - - - 0.129
MoO4
- - 9.52x10-4 - -
NO-3 0.0547 - 0.729 -
PO-3 3.442x10-3 0.146 0.0545 -
4
SiO3
-2 8.04x10-3 - - -

9. References
• Allen M. B., and Arnon D. L., Studies on Nitrogen-Fixing Blue-Green
Algae. I. Growth and Nitrogen Fixation by Anabaena Cylindrica Lemm.
Plant Physiology, 30 (1955) 366-372.
• Berberoglua H., Jayb J., and Pilona L., Effect of nutrient media on
photobiological hydrogen production by Anabaena variabilis ATCC 29413.
International Journal of Hydrogen Energy. 33 (2008 ) 1172 – 1184.
• Seto A., Wang H. L., and Hesseltine C.W., Culture Conditions Affect
Eicosapentaenoic Acid Content of ChIarella minutissima. JAOCS. 61 (1984)
892 – 894.
• Mann, J.E., and Myers, J., On pigments, growth and photosynthesis of
Phaeodactylum tricornutum. Journal of Phycology, 4 (1968) 349-355.
• Miron A. S., Go´mez A. C., Camacho F. G., Grima E. M., Chisti Y.
Comparative evaluation of compact photobioreactors for large-scale
monoculture of microalgae. Journal of Biotechnology, 70 (1999) 249–270.