Here we get most all things about fluorimetry used in pharmaceutical drug analysis

2. INTRODUCTION

5. Animation of principle of
Fluorescence and Phosphorescence
https://www.youtube.com/watch?v=AWkT9teuBlQ

10. EXAMPLE
Fluorescent paint
used on road sign
boards

12. Example
Radium used in watches
shows phosphorescence

14. EXCITATION

15. HOMO & LUMO
HOMO- Highest occupied
molecular orbital
LUMO- Lowest unoccupied
molecular orbital

26. PATH LENGTH

27. QUENCHING

32. INSTRUMENTATION

36. MERCURY ARC LAMP

40. TUNGSTEN LAMP

43. FILTERS
There are two filters for the fluorometer:
1. The primary filter or excitation filter or incident
light filter isolates the wavelength that will cause
the compound to fluoresce (the incident light).
2. The secondary filter isolates the desired
emitted light (fluorescent light).

45. M0N0CHROMATORS
A monochromator is an optical device that transmits a
mechanically selectable narrow band of wavelengths of light
or other radiation chosen from a wider range of wavelengths
available at the input.
Greek words
mono-, "single",
and chroma, "colour"

47. SAMPLE HOLDER / CUVETTE

53. PHOTOMULTIPLER TUBE

54. Animation of instrumentation of Fluorimetry
https://www.youtube.com/watch?v=zGFQEbdeAF0

56. SINGLE & DOUBLE BEAM
FLUORIMETER

62. APPLICATIONS OF FLUORIMETRY

66. TAPAN KUMAR MAHATO
tapan.mahato@gmail.com