7. Laser can only limit light of high
irradiance
Narrow wavelength
thus. makes excitation unnecessary.
Disadvantage:
wavelength can not vary much
8.
9. A. Excitation monochromators
B. Emission monochromators
• Filters or monochromator may be used in
fluorimeter
• A monochromator transmits light with
adjustable wavelength
• Ideal monochromator transmit light in the
specified range and high wavelength
• No monochromator is perfect ___transmit
some stray light
10. Single channel ;
Detect the intensity of one wavelength at a
time
Multi channel;
Detects intensity of all wavelengths
simultaneously, making the emission
monochromator or filter unnecessary.