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EI8551 ANALYTICAL INSTRUMENTS
UNIT I SPECTROPHOTOMETRY 9
Spectral methods of analysis – Beer-Lambert law – UV-Visible spectroscopy – IR
Spectrophotometry - FTIR spectrophotometry – Atomic absorption spectrophotometry -Flame emission
and atomic emission photometry – Construction, working principle, sources detectors and applications.
UNIT II CHROMATOGRAPHY 9
General principles – classification – chromatographic behavior of solutes – quantitative determination –
Gas chromatography – Liquid chromatography – High-pressure liquid chromatography – Applications.
UNIT III INDUSTRIAL GAS ANALYZERS AND POLLUTION MONITORING
INSTRUMENTS 9
Gas analyzers – Oxygen, NO2 and H2S types, IR analyzers, thermal conductivity detectors,analysis
based on ionization of gases.Air pollution due to carbon monoxide, hydrocarbons, nitrogen oxides,
sulphur dioxide estimation - Dust and smoke measurements.
UNIT IV pH METERS AND DISSOLVED COMPONENT ANALYZERS 9
Selective ion electrodes - Principle of pH and conductivity measurements – dissolved oxygen analyzer –
Sodium analyzer – Silicon analyzer – Water quality Analyzer.
UNIT V NUCLEAR MAGNETIC RESONANCE AND MASS SPECTROMETRY 9
NMR – Basic principles – Continuous and Pulsed Fourier Transform NMR spectrometer –Mass
Spectrometry – Sample system – Ionization methods – Mass analyzers – Types of mass spectrometry.
Analytical Instruments
•Spectrophotometer
•Conductivity Meter
•Calorimeter
•Flame Photometer
•Infrared Analyzer
•Oxygen Analyzers
•HPLC,
•Dissolved Oxygen Meter
•Mercury Analysers
•Thermometer
•Viscometer
•TOC Analyzer
•Titrators
•Polarimeter
•Air Sampling
•pH Meter
•Moisture Titration
•T.D.S. Meter
•Oscilloscopes
•
ELECTROMAGNETIC SPECTRUM
Wavelength of spectrum
Laws of Photometry
Beer Law
lamberts law
Beer lamberts Law
Beer lamberts Law
• Relation between Absorbance and Transmittance
Losses in light from I0 to I,
o Scattering by particles
o Reflection
o Absorption
Limitations to BLL
Types in Absorption spectrometer
• UV & VISIBLE
• Radiation source --- black body sources,LASER
• Discharge lamps
Selection of filters
Interference filter ----
Interference filter
• https://youtu.be/xHQM4BbR040 spectrophotometer
• https://youtu.be/51DSl8BHUjg hplc 1
• https://youtu.be/YaabRXcm4M4 hplc 2
• https://youtu.be/qdmKGskCyh8 thin layer chromatography

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UV and Visible Spectrometers

  • 1. EI8551 ANALYTICAL INSTRUMENTS UNIT I SPECTROPHOTOMETRY 9 Spectral methods of analysis – Beer-Lambert law – UV-Visible spectroscopy – IR Spectrophotometry - FTIR spectrophotometry – Atomic absorption spectrophotometry -Flame emission and atomic emission photometry – Construction, working principle, sources detectors and applications. UNIT II CHROMATOGRAPHY 9 General principles – classification – chromatographic behavior of solutes – quantitative determination – Gas chromatography – Liquid chromatography – High-pressure liquid chromatography – Applications. UNIT III INDUSTRIAL GAS ANALYZERS AND POLLUTION MONITORING INSTRUMENTS 9 Gas analyzers – Oxygen, NO2 and H2S types, IR analyzers, thermal conductivity detectors,analysis based on ionization of gases.Air pollution due to carbon monoxide, hydrocarbons, nitrogen oxides, sulphur dioxide estimation - Dust and smoke measurements. UNIT IV pH METERS AND DISSOLVED COMPONENT ANALYZERS 9 Selective ion electrodes - Principle of pH and conductivity measurements – dissolved oxygen analyzer – Sodium analyzer – Silicon analyzer – Water quality Analyzer. UNIT V NUCLEAR MAGNETIC RESONANCE AND MASS SPECTROMETRY 9 NMR – Basic principles – Continuous and Pulsed Fourier Transform NMR spectrometer –Mass Spectrometry – Sample system – Ionization methods – Mass analyzers – Types of mass spectrometry.
  • 2. Analytical Instruments •Spectrophotometer •Conductivity Meter •Calorimeter •Flame Photometer •Infrared Analyzer •Oxygen Analyzers •HPLC, •Dissolved Oxygen Meter •Mercury Analysers •Thermometer •Viscometer •TOC Analyzer •Titrators •Polarimeter •Air Sampling •pH Meter •Moisture Titration •T.D.S. Meter •Oscilloscopes •
  • 3.
  • 5.
  • 7. Laws of Photometry Beer Law lamberts law Beer lamberts Law
  • 8. Beer lamberts Law • Relation between Absorbance and Transmittance
  • 9. Losses in light from I0 to I, o Scattering by particles o Reflection o Absorption
  • 11.
  • 12. Types in Absorption spectrometer • UV & VISIBLE
  • 13. • Radiation source --- black body sources,LASER
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  • 36. • https://youtu.be/51DSl8BHUjg hplc 1 • https://youtu.be/YaabRXcm4M4 hplc 2 • https://youtu.be/qdmKGskCyh8 thin layer chromatography