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 The circulation of nitrogen in nature, consisting
of a cycle of chemical reactions in which
nitrogen from the atmosphere is fixed in
 Compounds in soil or water.
 Assimilated by plants and animals.
 Released to the soil and water
through decomposition
 Returned to the
atmosphere through Denitrification
Nitrogen cycle
 The continuous process by which nitrogen is excha
nged between organisms and the environment
 Some of the atmosphere's free nitrogen combines
with other elements to form compounds that are
deposited in the soil.
 These are then converted by bacteria, in process
called
nitrification, into nutrients that are absorbed by
the roots of green plants.
 Nitrogen is then passed into the food
chain and returned to the soil by metabolism a
nd decay of plants and animals.
 The growth of all organisms depends on the
availability of mineral nutrients, and none is more
important than nitrogen, which is required in large
amounts as an essential component of proteins, nucleic
acids and other cellular constituents.
 There is an abundant supply of nitrogen in the earth's
atmosphere - nearly 78% in the form of N2 gas.
However, N2 is unavailable for use by most organisms
because there is a triple bond between the two nitrogen
atoms, making the molecule almost inert.
 In order for nitrogen to be used for growth it must be
"fixed" (combined) in the form of ammonium (NH4) or
nitrate (NO3).
 Nitrogen-fixing bacteria, microorganisms
capable of transforming atmospheric nitrogen
into fixed nitrogen (inorganic compounds
usable by plants). More than 90 percent of all
nitrogen fixation is effected by these organisms,
which thus play an important role in
the nitrogen cycle.
Two kinds of nitrogen-fixing bacteria are
recognized.
The first kind, the free-living (nonsymbiotic)
bacteria, includes the cyanobacteria(or blue-
green algae) Anabaena and Nostoc and genera
such as Azotobacter and Clostridium.
The second kind comprises the mutualistic
(symbiotic) bacteria; examples
include Rhizobium, associated with leguminous
plants (e.g., various members of the pea family.
 The symbiotic nitrogen-fixing bacteria present in
the root hairs of host plants, where they multiply
and stimulate formation of root nodules,
enlargements of plant cells and bacteria in intimate
association. Within the nodules the bacteria
convert free nitrogen to ammonia which the host
plant utilizes for its development.
 Legumes (e.g., alfalfa, beans, peas, soybeans),
seeds are usually inoculated with commercial
cultures of appropriate Rhizobium species,
especially in soils poor or lacking in the required
bacterium.
 The term nitrification refers to the conversion of
ammonium to nitrate (pathway 3-4). This is brought
about by the nitrifying bacteria, which are specialized
to gain their energy by oxidizing ammonium, while
using CO2 as their source of carbon to synthesize
organic compounds
 The nitrifying bacteria are found in moist soils and
waters of moderate pH, but are not active in highly
acidic soils. They almost always are found as mixed-
species communities e.g. Nitrosomonasspecies - are
specialized to convert ammonium to nitrite (NO2
-)
while others - e.g. Nitrobacter species - convert nitrite to
nitrate (NO3
-
 Denitrification refers to the process in which nitrate is
converted to gaseous compounds (nitric oxide, nitrous
oxide and N2) by microorganisms. The sequence
usually involves the production of nitrite (NO2
-)
.Several types of bacteria perform this conversion when
growing on organic matter in anaerobic conditions.
Because of the lack of oxygen for normal aerobic
respiration, they use nitrate in place of oxygen as the
terminal electron acceptor.
 In the absence of oxygen, any reducible substance such
as nitrate (NO3
-) could serve the same role and be
reduced to nitrite, nitric oxide, nitrous oxide or N2.

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ubaid afzal

  • 1.  The circulation of nitrogen in nature, consisting of a cycle of chemical reactions in which nitrogen from the atmosphere is fixed in  Compounds in soil or water.  Assimilated by plants and animals.  Released to the soil and water through decomposition  Returned to the atmosphere through Denitrification
  • 2. Nitrogen cycle  The continuous process by which nitrogen is excha nged between organisms and the environment  Some of the atmosphere's free nitrogen combines with other elements to form compounds that are deposited in the soil.  These are then converted by bacteria, in process called nitrification, into nutrients that are absorbed by the roots of green plants.
  • 3.  Nitrogen is then passed into the food chain and returned to the soil by metabolism a nd decay of plants and animals.
  • 4.
  • 5.  The growth of all organisms depends on the availability of mineral nutrients, and none is more important than nitrogen, which is required in large amounts as an essential component of proteins, nucleic acids and other cellular constituents.  There is an abundant supply of nitrogen in the earth's atmosphere - nearly 78% in the form of N2 gas. However, N2 is unavailable for use by most organisms because there is a triple bond between the two nitrogen atoms, making the molecule almost inert.  In order for nitrogen to be used for growth it must be "fixed" (combined) in the form of ammonium (NH4) or nitrate (NO3).
  • 6.  Nitrogen-fixing bacteria, microorganisms capable of transforming atmospheric nitrogen into fixed nitrogen (inorganic compounds usable by plants). More than 90 percent of all nitrogen fixation is effected by these organisms, which thus play an important role in the nitrogen cycle.
  • 7. Two kinds of nitrogen-fixing bacteria are recognized. The first kind, the free-living (nonsymbiotic) bacteria, includes the cyanobacteria(or blue- green algae) Anabaena and Nostoc and genera such as Azotobacter and Clostridium. The second kind comprises the mutualistic (symbiotic) bacteria; examples include Rhizobium, associated with leguminous plants (e.g., various members of the pea family.
  • 8.  The symbiotic nitrogen-fixing bacteria present in the root hairs of host plants, where they multiply and stimulate formation of root nodules, enlargements of plant cells and bacteria in intimate association. Within the nodules the bacteria convert free nitrogen to ammonia which the host plant utilizes for its development.  Legumes (e.g., alfalfa, beans, peas, soybeans), seeds are usually inoculated with commercial cultures of appropriate Rhizobium species, especially in soils poor or lacking in the required bacterium.
  • 9.
  • 10.
  • 11.  The term nitrification refers to the conversion of ammonium to nitrate (pathway 3-4). This is brought about by the nitrifying bacteria, which are specialized to gain their energy by oxidizing ammonium, while using CO2 as their source of carbon to synthesize organic compounds  The nitrifying bacteria are found in moist soils and waters of moderate pH, but are not active in highly acidic soils. They almost always are found as mixed- species communities e.g. Nitrosomonasspecies - are specialized to convert ammonium to nitrite (NO2 -) while others - e.g. Nitrobacter species - convert nitrite to nitrate (NO3 -
  • 12.  Denitrification refers to the process in which nitrate is converted to gaseous compounds (nitric oxide, nitrous oxide and N2) by microorganisms. The sequence usually involves the production of nitrite (NO2 -) .Several types of bacteria perform this conversion when growing on organic matter in anaerobic conditions. Because of the lack of oxygen for normal aerobic respiration, they use nitrate in place of oxygen as the terminal electron acceptor.  In the absence of oxygen, any reducible substance such as nitrate (NO3 -) could serve the same role and be reduced to nitrite, nitric oxide, nitrous oxide or N2.