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NITROGEN CYCLE
Presented by Shanza Maryam
Introduction
Nitrogen is absolutely necessary for the
existence of life, as it is essential
component of proteins and nucleic
acids, which are vital to living
organisms.
 Luckily the earth’s atmosphere has
78.9% of nitrogen by volume
Although nitrogen is so important for
organisms and present in huge quantity
in atmosphere, it cannot be used by
plants and animals in gaseous and
elemental form.
Plants can only use nitrogen is present in
the form of nitrate(NO3), nitrite (NO2)
or ammonium ions(NH4).
The plants absorb nitrate compounds
and ammonium ions from the soil and
make proteins and nucleic acids.
whereas animals get their nitrogen
already fixed in plant tissues they eat.
The major reservoir of nitrogen is
atmosphere where nitrogen exist
Process of nitrogen cycle
Nitrogen cycle consists of following steps
namely:
1.Nitrogen Fixation
2.Assimilation
3.Ammonification/ Decay
4.Nitrification
5.De-nitrification
It is important to note that microorganisms play
an important role in each of these steps.
1.Nitrogen Fixation
 The conversion of free or gaseous
nitrogen into nitrate compounds or
ammonia is called Nitrogen
Fixation.
There are three principal ways in
which nitrogen fixation can occur.
(a)Atmospheric fixation:
When lightning or thunderstorms occur,
greater quantities of atmospheric
nitrogen are turned into nitrates or nitric
acid
 These are dissolved in rain and carried
to earth.
 Plants acquire these when they absorb
water and other minerals through their
roots.
(b)Biological fixation
Microorganisms capable of transforming
atmospheric nitrogen into fixed nitrogen
(inorganic compounds usable by plants).
More than 90 percent of all nitrogen
fixation is effected by these organisms,
which thus play an important role in the
nitrogen cycle.
 Two kinds of nitrogen-fixing bacteria
are recognized. The first kind, the
free-living (nonsymbiotic) bacteria,
includes the cyanobacteria Anabaena
and Nostoc.
 The second kind comprises the
mutualistic (symbiotic) bacteria;
examples include Rhizobium,
The symbiotic nitrogen-
fixing bacteria invade the
root hairs of host plants,
where they multiply and
stimulate formation of root
nodules,Within the nodules
the bacteria convert free
nitrogen to ammonia, which
the host plant utilizes for its
development.
Nitrogenase is an
enzyme responsible for
catalyzing nitrogen
fixation, Nitrogenase
catalyzes the breaking
of triple bonds between
nitrogen atoms and the
addition of three
hydrogen atoms to each
nitrogen atom.
(c)Industrial fixation
It is a man-made alternative that
aids in nitrogen fixation by the use
of ammonia. Ammonia is produced
by the direct combination of
nitrogen and hydrogen. Later, it is
converted into various fertilizers
such as urea.
2.Assimilation
Once the nitrogen has been fixed in
the soil, plants can absorb nitrogen
through their roots. This process of
absorption is known as assimilation.
3.Ammonification
This is another process by which ammonia
can be generated. Organic remains of
plants and animals are broken down in the
soil by some bacteria to release ammonia
into the soil. These dead and waste matter
is used by these microorganisms as food
and they release ammonia into the soil
4. Nitrification
Nitrification is a two-step process in
which NH3/ NH4+ is converted to NO3– .
First, the soil bacteria Nitrosomonas and
Nitrococcus convert NH3 to NO2–
(nitrites), and then another soil
bacterium, Nitrobacter, oxidizes NO2– to
NO3–(nitrates).
The reaction involved in the process of Nitrification is as
follows:
2NH3 + 3O2 → 2NO2
– + 2H+ + 2H2O
2NO2
– + O2 → 2NO3
–
6.Denitrification
Most nitrate or ammonia compounds in
the soil are reduced again to free
molecular nitrogen (N2) which is released
back to the atmosphere. Reduction of
nitrates into free molecular nitrogen is
called De-nitrification.
Nitrogen Cycle_ SlideShare
Nitrogen Cycle_ SlideShare

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Nitrogen Cycle_ SlideShare

  • 2. Introduction Nitrogen is absolutely necessary for the existence of life, as it is essential component of proteins and nucleic acids, which are vital to living organisms.  Luckily the earth’s atmosphere has 78.9% of nitrogen by volume
  • 3. Although nitrogen is so important for organisms and present in huge quantity in atmosphere, it cannot be used by plants and animals in gaseous and elemental form. Plants can only use nitrogen is present in the form of nitrate(NO3), nitrite (NO2) or ammonium ions(NH4).
  • 4. The plants absorb nitrate compounds and ammonium ions from the soil and make proteins and nucleic acids. whereas animals get their nitrogen already fixed in plant tissues they eat. The major reservoir of nitrogen is atmosphere where nitrogen exist
  • 5. Process of nitrogen cycle Nitrogen cycle consists of following steps namely: 1.Nitrogen Fixation 2.Assimilation 3.Ammonification/ Decay 4.Nitrification 5.De-nitrification It is important to note that microorganisms play an important role in each of these steps.
  • 6. 1.Nitrogen Fixation  The conversion of free or gaseous nitrogen into nitrate compounds or ammonia is called Nitrogen Fixation. There are three principal ways in which nitrogen fixation can occur.
  • 7. (a)Atmospheric fixation: When lightning or thunderstorms occur, greater quantities of atmospheric nitrogen are turned into nitrates or nitric acid  These are dissolved in rain and carried to earth.  Plants acquire these when they absorb water and other minerals through their roots.
  • 8. (b)Biological fixation Microorganisms capable of transforming atmospheric nitrogen into fixed nitrogen (inorganic compounds usable by plants). More than 90 percent of all nitrogen fixation is effected by these organisms, which thus play an important role in the nitrogen cycle.
  • 9.  Two kinds of nitrogen-fixing bacteria are recognized. The first kind, the free-living (nonsymbiotic) bacteria, includes the cyanobacteria Anabaena and Nostoc.  The second kind comprises the mutualistic (symbiotic) bacteria; examples include Rhizobium,
  • 10. The symbiotic nitrogen- fixing bacteria invade the root hairs of host plants, where they multiply and stimulate formation of root nodules,Within the nodules the bacteria convert free nitrogen to ammonia, which the host plant utilizes for its development.
  • 11. Nitrogenase is an enzyme responsible for catalyzing nitrogen fixation, Nitrogenase catalyzes the breaking of triple bonds between nitrogen atoms and the addition of three hydrogen atoms to each nitrogen atom.
  • 12. (c)Industrial fixation It is a man-made alternative that aids in nitrogen fixation by the use of ammonia. Ammonia is produced by the direct combination of nitrogen and hydrogen. Later, it is converted into various fertilizers such as urea.
  • 13. 2.Assimilation Once the nitrogen has been fixed in the soil, plants can absorb nitrogen through their roots. This process of absorption is known as assimilation.
  • 14. 3.Ammonification This is another process by which ammonia can be generated. Organic remains of plants and animals are broken down in the soil by some bacteria to release ammonia into the soil. These dead and waste matter is used by these microorganisms as food and they release ammonia into the soil
  • 15.
  • 16. 4. Nitrification Nitrification is a two-step process in which NH3/ NH4+ is converted to NO3– . First, the soil bacteria Nitrosomonas and Nitrococcus convert NH3 to NO2– (nitrites), and then another soil bacterium, Nitrobacter, oxidizes NO2– to NO3–(nitrates).
  • 17. The reaction involved in the process of Nitrification is as follows: 2NH3 + 3O2 → 2NO2 – + 2H+ + 2H2O 2NO2 – + O2 → 2NO3 –
  • 18. 6.Denitrification Most nitrate or ammonia compounds in the soil are reduced again to free molecular nitrogen (N2) which is released back to the atmosphere. Reduction of nitrates into free molecular nitrogen is called De-nitrification.