root regeneration from callus

1. Root regeneration from callus

2. Contents
 Regeneration
 Root regeneration by tissue culture
 Basic requirements for root regeneration
 Callus
 Media
 Phytohormones required
 De novo organogenesis

3. What is regeneration?
The process of growing an
entire organism or any part of
organism from a single cell or
group of cells.

4. Root regeneration
root is underground part of plant
that arise from radical usually
colourless and do not contain
leaves
Root can be regenerate by tissue
culture techniques from a single
cell

5. Requirements for root regeneration
Root formation required certain
requirements
Callus
Callus is an undifferentiated mass of
cells from which root can generate

6. Media

7. Types of media
 White’s medium:
This is one of the earliest plant tissue culture media
developed for root culture.
 MS medium:
Murashige and Skoog (MS) originally formulated a medium
to induce organogenesis, and regeneration of plants in
cultured tissues

8. Nutrients
 Macronutrients:
N, P, K, Ca, S, Mg, C,O , H
 Micronutrients :
Fe, B, Cl, Mn, Zn, Cu, Mo
Phosphorus plays an key role in root development.

10.  Phytohormones
Auxins play a central role in root
regeneration
Cytokinin act as an auxin
antagonist

12.  Carbon and energy source
(sugars)
 Organic supplements
( vitamins, amino acids, organic acids etc)
 Solidifying agents
(agar, gelatin)
 pH of medium
(5-6)

13.  callus can leads towards the embryogenesis or
organogenesis
 The organogenesis can further leads to root or shoot
formation depending upon the availability of plant growth
regulators either auxin or cytikinin

14. De novo organogenesis
De novo root organogenesis is the
process in which adventitious roots
regenerate from detached or wounded
plant tissues or organs. In tissue culture,
appropriate types and concentrations of
plant hormones in the medium are
critical for inducing adventitious roots.

15. THANK YOU