This document discusses plant tissue culture, which involves growing plant cells, tissues, or organs under sterile conditions using a nutrient culture medium. Plant tissue culture is used to produce clones of plants through a process called micropropagation. It describes how callus formation is induced from plant tissue samples by growing unorganized plant cells. The key equipment used includes conical containers, forceps, agar, sucrose, plant growth regulators, and an autoclave for sterilizing explants and equipment prior to culturing plant cells and tissues to form callus or entire new plants.

2.  A collection of techniques used to maintain or
grow plant cells, tissues or organs
 Under sterile conditions on a
nutrient culture medium
 Used to produce clones of a plant in a method
known as micropropagation.

4.  a growing mass
 unorganized plant parenchyma cells.
 callus formation is induced from plant tissue
samples (explants)

6.  The process of growing an entire plant from a
single cell or group of cells.
 the physiological renewal, repair, or
replacement of tissue in plants.

7.  conical containers
 Forceps
 Magnetic stirrer
 Agar
 sucrose
 plant growth regulators
 aluminum foil

8.  Scissors
 Knife
 Beakers
 sodium hypochlorite solution
 70% alcohol

9.  Laminar Flow Cabinet
 Autoclave
 Magnetic stirrer
 Oven
 Sprit lamp

10.  Choose an explant
 Wash it with tap water for 10 minutes
 Place it in 1% sodium hypochlorite solution
 Wash it with distilled water
 All the equipments are sterilized by placing in
autoclave

11.  Then the explant with the nutrient media is
place din the laminar flow air cabinet
 After callus formation from explants the
formed calli are transferred into conical
containers
 Levels were used as cytokinin and auxin
sources