There are three main types of cell culture: primary cell culture, where cells are directly separated from tissue and grown; secondary cell culture, where primary cells are subcultured and transferred to new vessels; and cell lines, which can be either finite, with a limited lifespan, or continuous, capable of indefinite growth. Primary cell culture can involve adherent cells that attach to surfaces or suspension cells that float freely. Secondary culture involves detaching and transferring adherent cells to new vessels using enzymatic methods.

1. TYPES CELL CULTURE
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2. TYPES OF CELL CULTURE
Cell culture is classified into three:
i. Primary cell culture
• Adherent cell culture
• Suspension cell culture
ii. Secondary cell culture
iii. Cell line
• Finite cell line
• Continuous cell line

3. TYPES OF CELL CULTURE
Cell culture is classified into three:
i. Primary cell culture
• Adherent cell culture
• Suspension cell culture
ii. Secondary cell culture
iii. Cell line
• Finite cell line
• Continuous cell line

4. PRIMARY CELL CULTURE
Separate cell directly from the parent tissue ( kidney, liver, heart etc.)
Separated by enzymatic or mechanical method.
Maintaining the growth of the cell in a culture medium using glass or plastic
container.
Culture medium
o Liquid or gel designed to maintain the growth of the cell.
o It varies from different type of cell.

5. PRIMARY CELL CULTURE
Primary cell culture is further classified based on the type of cell:
i. Adherent cell :
• Those cell which attach to the surface of the culture flask.
• Forms a monolayer.
• They have to be detached from the surface before they get sub cultured.
• Growth limited to surface area.
ii. Suspension cell
• Those cell does not get attached to the surface of culture flask.
• They are free floating.
• Cells in blood stream.
• Growth is limited to concentration of cells.

6. SECONDARY CELL CULTURE
When primary cell is sub cultured then it is secondary cell.
Sub culture;
 Transfer of cell from one culture vessel to another.
 Method:
o Remove growth medium.
o Detach adherent cells (enzymatic method)
 Why required:
o To provide fresh nutrients.
o To provide more space.

7. CELL LINE
Finite cell lines
 Limited life span
 Go through a limited number of cell generation
 Properties:
o Contact inhibition
o Density limitation
o Anchorage dependence
 Less growth rate
 Doubling time (24-92hrs.)
Continuous cell lines
Grow indefinitely
Grows either in monolayer or in suspension
Properties:
o Absence Contact inhibition
o Absence Anchorage dependence
High growth rate
Doubling time (12-24hrs.)