Cell culture media can be natural or artificial. Natural media include coagula/clots, biological fluids, and tissue extracts. Artificial (synthetic) media include serum-containing, serum-free, and chemically defined media. Serum-containing media supplement defined media like Eagle Minimum Essential Medium with 5-20% serum, which provides nutrients, hormones, growth factors, and other important components to support cell growth. However, serum can also inhibit some cell types and contain cytotoxic constituents. Serum-free media avoid these disadvantages but may be specific to cell types and require close control of conditions. Chemically defined media use ultrapure inorganic and organic components and defined protein additives produced through genetic engineering.
Constituent of animal tissue culture media and their specific applicationKAUSHAL SAHU
INTRODUCTION
HISTORY
PHYSICOCHEMICAL PROPERTIES OF CULTURE MEDIA
pH
CO2, BICARBONATE AND BUFFERING
OXYGEN
TEMPERATURE
OSMOLALITY
BALANCED SALT SOLUTIONS
CONSTITUENTS OF CULTURE MEDIA
AMINO ACIDS
VITAMINS
SALTS
GLUCOSE
OTHER ORGANIC SUPPLEMENTS
ANTIBIOTICS
SERUM
PROTEINS
NUTRIENTS AND METABOLITES
HORMONES AND GROWTH FACTORS
LIPIDS
MINERALS
INHIBITORS
APPLICATIONS OF CULTURE MEDIA
CONCLUSION
REFERENCES
Introduction
Primary Culture
Steps In Primary Culture
Isolation Of Tissue
Dissection And/Or Disaggregation
Types Of Primary Culture
Primary Explant Culture
Enzymatic Disaggregation
Mechanical Disaggregation
Cell Line( Finite & Continuous)
Naming A Cell Line
Choosing A Cell Line
Maintenance Of Cell Line
Conclusion
reference
Constituent of animal tissue culture media and their specific applicationKAUSHAL SAHU
INTRODUCTION
HISTORY
PHYSICOCHEMICAL PROPERTIES OF CULTURE MEDIA
pH
CO2, BICARBONATE AND BUFFERING
OXYGEN
TEMPERATURE
OSMOLALITY
BALANCED SALT SOLUTIONS
CONSTITUENTS OF CULTURE MEDIA
AMINO ACIDS
VITAMINS
SALTS
GLUCOSE
OTHER ORGANIC SUPPLEMENTS
ANTIBIOTICS
SERUM
PROTEINS
NUTRIENTS AND METABOLITES
HORMONES AND GROWTH FACTORS
LIPIDS
MINERALS
INHIBITORS
APPLICATIONS OF CULTURE MEDIA
CONCLUSION
REFERENCES
Introduction
Primary Culture
Steps In Primary Culture
Isolation Of Tissue
Dissection And/Or Disaggregation
Types Of Primary Culture
Primary Explant Culture
Enzymatic Disaggregation
Mechanical Disaggregation
Cell Line( Finite & Continuous)
Naming A Cell Line
Choosing A Cell Line
Maintenance Of Cell Line
Conclusion
reference
Media is one of the important components for in vitro cultivation of animal cells. Every animal cells have specific requirements and media are designed by keeping in mind those requirements. However, the basic components and design principle remains the same. Every cell culture media contain carbon source, nitrogen source, trace elements, pH indicator, antibiotics ( although it is not recommended) for high value cell culture applications. While designing media various aspects are considered such as availability, cost effectiveness, types off cells to be grown and regulatory requirements. Tis slide also contains sample MCQs questions
Equipments used , types of culture and media, subculturing, secondary culture, finite & continuous cell lines, cryopreservation and applications of cell culture
Primary and established cell line cultureKAUSHAL SAHU
Introduction
Primary Culture
Steps of Primary Culture
Isolation Of Tissue
Dissection And Disaggregation
Types Of Primary Culture
Primary Explants Culture
Enzymatic Disaggregation
Mechanical Disaggregation
Cell Line( Finite & Continuous)
Naming A Cell Line
Choosing A Cell Line
Maintenance Of Cell Line
Conclusion
Reference
Biology and characterization of the cell cultureKAUSHAL SAHU
Introduction
History
Important terminology
Biology of culture cell
Characterization of culture cell
Application of animal culture
Conclusion
References
Animal cell culture, application by kk sahuKAUSHAL SAHU
INTRODUCTION
HISTORY
CELL CULTURE IN TWO DIMENSION
CELL CULTURE IN THREE DIMENSION
APPLICATION:-
VACCINES
PRODUCTION OF HIGH VALUE THERAPEUTICS
TRANSGENIC ANIMAL
GENE THERAPY
TISSUE ENGINEERING
CONCLUSION
REFRENCES
Introduction
Terminologies
Types of tissue culture
Applications
Culturing
Sub-culturing
Cryopreservation
Detection of contaminants
In vitro transformation of cells
Cell viability
Rules for working in the Lab
Advantages
Limitations
In vitro methods for the assessment of general cellular toxicity,
End-points for the assessment of general cellular toxicity
Specialized cells commonly used in toxicology
Media is one of the important components for in vitro cultivation of animal cells. Every animal cells have specific requirements and media are designed by keeping in mind those requirements. However, the basic components and design principle remains the same. Every cell culture media contain carbon source, nitrogen source, trace elements, pH indicator, antibiotics ( although it is not recommended) for high value cell culture applications. While designing media various aspects are considered such as availability, cost effectiveness, types off cells to be grown and regulatory requirements. Tis slide also contains sample MCQs questions
Equipments used , types of culture and media, subculturing, secondary culture, finite & continuous cell lines, cryopreservation and applications of cell culture
Primary and established cell line cultureKAUSHAL SAHU
Introduction
Primary Culture
Steps of Primary Culture
Isolation Of Tissue
Dissection And Disaggregation
Types Of Primary Culture
Primary Explants Culture
Enzymatic Disaggregation
Mechanical Disaggregation
Cell Line( Finite & Continuous)
Naming A Cell Line
Choosing A Cell Line
Maintenance Of Cell Line
Conclusion
Reference
Biology and characterization of the cell cultureKAUSHAL SAHU
Introduction
History
Important terminology
Biology of culture cell
Characterization of culture cell
Application of animal culture
Conclusion
References
Animal cell culture, application by kk sahuKAUSHAL SAHU
INTRODUCTION
HISTORY
CELL CULTURE IN TWO DIMENSION
CELL CULTURE IN THREE DIMENSION
APPLICATION:-
VACCINES
PRODUCTION OF HIGH VALUE THERAPEUTICS
TRANSGENIC ANIMAL
GENE THERAPY
TISSUE ENGINEERING
CONCLUSION
REFRENCES
Introduction
Terminologies
Types of tissue culture
Applications
Culturing
Sub-culturing
Cryopreservation
Detection of contaminants
In vitro transformation of cells
Cell viability
Rules for working in the Lab
Advantages
Limitations
In vitro methods for the assessment of general cellular toxicity,
End-points for the assessment of general cellular toxicity
Specialized cells commonly used in toxicology
A growth medium or culture medium is a liquid or gel designated to support the growth of microorganisms,cells,or small plants.
Culture media generally comprise an appropriate sourcde of energy and compounds which regulate the cell cycle.
A typical culture media is composed of a complement of amino acids,vitamins,inorganic salts,glucose, and serum as a source of growth factors, hormones, and attachment factors.
In addition to nutrients, the medium also helps maintain pH and osmolarity.
Imporatant amino acids, trace elements, growth factor,hormone,transport protein and adhesion factor are added.
Adhesion factor added are main components of intercellular substance such as fibronectin,collagen and laminin.
Primary purpose of introducing SFM is to promote the specific growth of a particular type of cell.
Exapmple of one serum free media: DCMM-1 SFM. High protein serum free medium, designed for hybridoma cell growth and monoclonal antibody production.
Animal Cell culture by S.D.Mankar, Pravara Rural college of Pharmacysomeshwar mankar
Growth of animal cells in culture, general procedure for cell culture,
Primary, established and transformed cell cultures.
Application of cell cultures in pharmaceutical industry and research.
1. History of Cell Culture
2. Introduction to cell culture
3. types of cell lines
4. culture media
5. serum in culture media
6. Applications of cell & tissue culture
7. Adherence
8. Cell line evolution
9. Passaging, revival and cryopreservation
10. cell culture laboratory layout
this slide will help you to understand the behavior of different types of bacteria in different culture media. its is made with an exmaple experiment which can provide better understadng. selective, differential and enriched media is given with detailed description in the example.
Students, digital devices and success - Andreas Schleicher - 27 May 2024..pptxEduSkills OECD
Andreas Schleicher presents at the OECD webinar ‘Digital devices in schools: detrimental distraction or secret to success?’ on 27 May 2024. The presentation was based on findings from PISA 2022 results and the webinar helped launch the PISA in Focus ‘Managing screen time: How to protect and equip students against distraction’ https://www.oecd-ilibrary.org/education/managing-screen-time_7c225af4-en and the OECD Education Policy Perspective ‘Students, digital devices and success’ can be found here - https://oe.cd/il/5yV
We all have good and bad thoughts from time to time and situation to situation. We are bombarded daily with spiraling thoughts(both negative and positive) creating all-consuming feel , making us difficult to manage with associated suffering. Good thoughts are like our Mob Signal (Positive thought) amidst noise(negative thought) in the atmosphere. Negative thoughts like noise outweigh positive thoughts. These thoughts often create unwanted confusion, trouble, stress and frustration in our mind as well as chaos in our physical world. Negative thoughts are also known as “distorted thinking”.
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
This is a presentation by Dada Robert in a Your Skill Boost masterclass organised by the Excellence Foundation for South Sudan (EFSS) on Saturday, the 25th and Sunday, the 26th of May 2024.
He discussed the concept of quality improvement, emphasizing its applicability to various aspects of life, including personal, project, and program improvements. He defined quality as doing the right thing at the right time in the right way to achieve the best possible results and discussed the concept of the "gap" between what we know and what we do, and how this gap represents the areas we need to improve. He explained the scientific approach to quality improvement, which involves systematic performance analysis, testing and learning, and implementing change ideas. He also highlighted the importance of client focus and a team approach to quality improvement.
The Indian economy is classified into different sectors to simplify the analysis and understanding of economic activities. For Class 10, it's essential to grasp the sectors of the Indian economy, understand their characteristics, and recognize their importance. This guide will provide detailed notes on the Sectors of the Indian Economy Class 10, using specific long-tail keywords to enhance comprehension.
For more information, visit-www.vavaclasses.com
The Roman Empire A Historical Colossus.pdfkaushalkr1407
The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
The Art Pastor's Guide to Sabbath | Steve ThomasonSteve Thomason
What is the purpose of the Sabbath Law in the Torah. It is interesting to compare how the context of the law shifts from Exodus to Deuteronomy. Who gets to rest, and why?
2. CELL CULTURE MEDIA
A Culture media is a nutrient solution containing all
essential organic and inorganic ingredients required for
the growth of animal cell.
Various types of media used for tissue culture may be
grouped into two broad categories.
1. Natural media and
2. Artificial media
3. CELL CULTURE MEDIA
NATURAL MEDIA
These media consist of naturally occurring
biological fluids and these are the following three
types
a. Coagula/Clots
b. Biological fluids
c. Tissue extracts
4. CELL CULTURE MEDIA
a. Coagula/Clots:
Most commonly used clots are plasma clots,
which are being used since long time.
Plasma is now commercially available either in
liquid or lyophilized state.
It may be prepared in the laboratory, usually from
blood of male fowl, but clotting must be avoided
during the preparation.
5. CELL CULTURE MEDIA
b. Biological fluids:
Of the various biological fluids used as Culture
media e.g. Amniotic fluid, Ascitic fluid, Pleural
fluid, Aqueous fluid from eye, insect hemolymph,
Serum etc.
Serum is the most widely used. It may be
obtained from human blood, placental cord
blood, horse blood, calf blood.
6. CELL CULTURE MEDIA
c. Tissue extracts:
Chick embryo extracts is the most
commonly used tissue extracts
but bovine embryo extracts are also used
other tissue extracts that have been used
are Spleen, liver, bone marrow, leucocytes
etc. extracts.
7. CELL CULTURE MEDIA
ARTIFICIAL MEDIA OR SYNTHETIC MEDIA
Various artificial media may be
grouped into following types.
Serum containing media
Serum free media and
Chemically defined media
8. CELL CULTURE MEDIA
a. Serum containing media:
Various defined media, e.g. Eagle Minimum Essential
Medium (EMEM) etc., When supplemented with 5-20%
serum becomes good nutrient medium for culture of most
types of cells. Serum serves the following major functions.
It provides the basic nutrients for cell. The nutrients are
present both in the solution and are bound to the
proteins.
It provides hormones like insulin, which is essential for
growth of nearly all cells in culture.
9. CELL CULTURE MEDIA
It contains several growth factors like Platelet –
derived growth factor, transforming growth factor,
epidermal growth factor, fibroblast growth factor,
endothelial growth factor etc.
A major role of serum is to supply proteins. Like
fibronectin, to promote attachment of cells to the
substrate.
It provides several binding proteins like Albumin,
Transferrin etc. which carry other molecules into the
cell.
10. CELL CULTURE MEDIA
It increases the viscosity of medium and
thereby, protects cells from mechanical
damages.
The serum also provides several minerals
like Na+, k+, Fe+, Zn+, Cu++, etc.
It also acts as a buffer.
11. CELL CULTURE MEDIA
Disadvantages of using serum in the culture media
Serum may inhibit growth of some cell types such
as epidermal keratinocytes.
Serum may contain potentially cytotoxic
constituents. Ex: feotal calf serum contain the
enzyme polyamine oxidase, which converts
polyamines like spermidine and spermine into
cytotoxic poly amino aldehydes.
12. CELL CULTURE MEDIA
b. Serum free media:
In view of the disadvantages due to serum,
extensive investigations have been made to develop
serum free formulations of culture media.
Improved reproducibility of results from different
laboratories.
Easier downstream processing of products from
cultured cells.
13. CELL CULTURE MEDIA
Toxic effects of serum are avoided.
There is no danger of degradation of serum
proteins.
Permit selective culture of differentiated
cells and produce cell types from the
heterogenous cultures.
14. CELL CULTURE MEDIA
Disadvantages:
Most serum free media are specific to one cell type.
Therefore, different media may be required for different
cell lines.
Reliable serum-free media preparation, for most of the
media formulations are not available commercially.
A greater control of pH, temperature etc. is necessary as
compared with serum containing media.
Growth rate and the maximum cell density attained are
lower than those with serum containing media.
15. CELL CULTURE MEDIA
c. Chemically defined media:
These media contain contamination-
free ultrapure inorganic and organic
constituents, besides pure protein additives
like insulin, epidermal growth factor etc.
that have been produced in bacteria or
yeast by genetic engineering methods.
16. This Photo by Unknown Author is licensed under CC BY
