This document discusses the transmission electron microscope (TEM). It begins by comparing light microscopes and electron microscopes. It then describes the key components of a TEM, including the electron source, lenses, vacuum chamber, and screen. It explains that TEMs produce high-resolution black and white images through the interaction of electrons with prepared samples. Applications of TEM include studying crystals, metals, and semiconductor analysis. Advantages are high-quality images and structure information, while disadvantages include large size, expense, specialized training and sample preparation.
1) CONTENTS:
Introduction
Construction
Working Principle
The Electron Gun And Condenser System
Image Producing & Recording System
TEM Applications
Advantages
Disadvantages
2) INTRODUCTION:
A Transmission Electron Microscope (TEM) utilizes energetic electron beam to provide morphologic, compositional and crystallographic information on samples.TEM produce High-Resolution, 2D images. The first transmission electron microscope was invented in 1933 by Max Knoll and E. Ruska at the Technical College in Berlin.
3) CONSTRUCTION:
Electron Gun – to produce electrons.
Magnetic condensing lens - to condense the electrons and to adjust the spot size of the electron.The specimen is placed in between the condensing lens and the objective lens.
The magnetic objective lens - to block the high angle diffracted
beam.
Aperture - eliminate the diffracted beam (if any) and in turn
increases the contrast of the image.The magnetic projector lens - to achieve higher magnification.
Fluorescent (Phosphor) screen – To record the image.
4)Working Principle: High voltage electron beam is transmitted through a specimen to form an image. Stream of electrons are produced by the electron gun and is made to fall over the specimen using the magnetic condensing lens.Electrons are made to pass through the specimen and the image is formed on the fluorescent screen.
5) The Electron Gun And Condenser System: The image can be manipulated by adjusting the voltage of the gun to accelerate or decrease the speed of electrons as well as changing the electromagnetic wavelength via the solenoids.
6) Image Producing & Recording System:
Air needs to be pumped out of the vacuum chamber, creating a
space where electrons are able to move.The objective lens is used to produces a image and then further magnified by the projector lens. The lighter areas of the image represent the places where a greater number of electrons were able to pass through the sample and the darker areas reflect the dense areas of the object. Monochromatic image is recorded in fluorescent screen or by capturing the image digitally to display on a computer monitor,basically stored in a TIFF or JPEG format.
7)TEM Applications:
It analyze structure, topographical, morphological, compositional and crystalline information. Can be used in semiconductor analysis and production and the manufacturing of computer and silicon chips. To identify fractures and damages.
8)Advantages:
Powerful magnification . It can produce magnification as high as 1,00,000 times as that of the size of the object.
Images are high-quality and detailed.They are easy to operate with proper training.
9)Disadvantages:
Large and very expensive.
Laborious sample preparation.
TEM require special housing and maintenance.
Samples are limited to those that are electron transparent.
10) Thank You
1) CONTENTS:
Introduction
Construction
Working Principle
The Electron Gun And Condenser System
Image Producing & Recording System
TEM Applications
Advantages
Disadvantages
2) INTRODUCTION:
A Transmission Electron Microscope (TEM) utilizes energetic electron beam to provide morphologic, compositional and crystallographic information on samples.TEM produce High-Resolution, 2D images. The first transmission electron microscope was invented in 1933 by Max Knoll and E. Ruska at the Technical College in Berlin.
3) CONSTRUCTION:
Electron Gun – to produce electrons.
Magnetic condensing lens - to condense the electrons and to adjust the spot size of the electron.The specimen is placed in between the condensing lens and the objective lens.
The magnetic objective lens - to block the high angle diffracted
beam.
Aperture - eliminate the diffracted beam (if any) and in turn
increases the contrast of the image.The magnetic projector lens - to achieve higher magnification.
Fluorescent (Phosphor) screen – To record the image.
4)Working Principle: High voltage electron beam is transmitted through a specimen to form an image. Stream of electrons are produced by the electron gun and is made to fall over the specimen using the magnetic condensing lens.Electrons are made to pass through the specimen and the image is formed on the fluorescent screen.
5) The Electron Gun And Condenser System: The image can be manipulated by adjusting the voltage of the gun to accelerate or decrease the speed of electrons as well as changing the electromagnetic wavelength via the solenoids.
6) Image Producing & Recording System:
Air needs to be pumped out of the vacuum chamber, creating a
space where electrons are able to move.The objective lens is used to produces a image and then further magnified by the projector lens. The lighter areas of the image represent the places where a greater number of electrons were able to pass through the sample and the darker areas reflect the dense areas of the object. Monochromatic image is recorded in fluorescent screen or by capturing the image digitally to display on a computer monitor,basically stored in a TIFF or JPEG format.
7)TEM Applications:
It analyze structure, topographical, morphological, compositional and crystalline information. Can be used in semiconductor analysis and production and the manufacturing of computer and silicon chips. To identify fractures and damages.
8)Advantages:
Powerful magnification . It can produce magnification as high as 1,00,000 times as that of the size of the object.
Images are high-quality and detailed.They are easy to operate with proper training.
9)Disadvantages:
Large and very expensive.
Laborious sample preparation.
TEM require special housing and maintenance.
Samples are limited to those that are electron transparent.
10) Thank You
5. Electron spectroscopy for surface analysis.
Applications of scanning electron microscope Applications of Transmission electron Microscope Brief history of electron microscopy Coherency and stability on the electron beam Different kinds of electron microscopes Different parts of electron microscope Effect of Brightness Electron Microscopy Electron Sources Field emission Interaction of electrons with Matter Limitations of Transmission electron Microscope Magnification contrast etc Material Characterization techniques Resolution Scanning electron microscope Scattering of electrons Specimen preparation of Transmission electron Microscope Thermionic Emission Various sources of electron beams and Detectors
Electron microscope, principle and applicationKAUSHAL SAHU
Introduction
History
Resolution &Magnification of
Electron microscope
Types of electron microscope
1) Transmission electron microscope (TEM)
- Structural parts of TEM
- Principle & Working of TEM
- Sample preparation for TEM
- Advantages & disadvantages of TEM
Scanning electron microscope (SEM)
- Structural parts of SEM
- Principle & Working of SEM
- Sample preparation for SEM
- Advantages & disadvantages of SEM
3) Scanning transmission electron microscope (STEM)
Applications of electron microscope
Conclusion
References
5. Electron spectroscopy for surface analysis.
Applications of scanning electron microscope Applications of Transmission electron Microscope Brief history of electron microscopy Coherency and stability on the electron beam Different kinds of electron microscopes Different parts of electron microscope Effect of Brightness Electron Microscopy Electron Sources Field emission Interaction of electrons with Matter Limitations of Transmission electron Microscope Magnification contrast etc Material Characterization techniques Resolution Scanning electron microscope Scattering of electrons Specimen preparation of Transmission electron Microscope Thermionic Emission Various sources of electron beams and Detectors
Electron microscope, principle and applicationKAUSHAL SAHU
Introduction
History
Resolution &Magnification of
Electron microscope
Types of electron microscope
1) Transmission electron microscope (TEM)
- Structural parts of TEM
- Principle & Working of TEM
- Sample preparation for TEM
- Advantages & disadvantages of TEM
Scanning electron microscope (SEM)
- Structural parts of SEM
- Principle & Working of SEM
- Sample preparation for SEM
- Advantages & disadvantages of SEM
3) Scanning transmission electron microscope (STEM)
Applications of electron microscope
Conclusion
References
Electron Microscopy - Scanning electron microscope, Transmission Electron Mic...Sumer Pankaj
An electron microscope is a microscope that uses a beam of accelerated electrons as a source of illumination. As the wavelength of an electron can be up to 100,000 times shorter than that of visible light photons, electron microscopes have a higher resolving power than light microscopes and can reveal the structure of smaller objects. A transmission electron microscope can achieve better than 50 pm resolution and magnifications of up to about 10,000,000x whereas most light microscopes are limited by diffraction to about 200 nm resolution and useful magnifications below 2000x.
Electron microscopes are used to investigate the ultrastructure of a wide range of biological and inorganic specimens including microorganisms, cells, large molecules, biopsy samples, metals, and crystals. Industrially, electron microscopes are often used for quality control and failure analysis. Modern electron microscopes produce electron micrographs using specialized digital cameras and frame grabbers to capture the image.
Optical microscopes use visible light and lenses to magnify specimens up to around 1000x, commonly applied in biology and materials science. They provide ease of use and minimal sample preparation. In contrast, electron microscopes, employing a beam of electrons, offer much higher magnifications and resolutions, reaching into the nanoscale. Transmission electron microscopes reveal internal structures, while scanning electron microscopes produce detailed 3D surface images. However, electron microscopes necessitate elaborate sample preparation, limiting their application to thin, metal-coated specimens. They are essential for exploring ultrastructural details in fields like cell biology and materials science.
This presentation include information about electron microscope & types of electron microscope i.e. SEM (Scanning electron microscope) & TEM (Transmission electron microscope).
An electron microscope is a microscope that uses a beam of scattered electrons as a source of illumination. It is used to get information about structure, topology, morphology & composition of materials. It has many advantages. Basically there are 4 types of electron microscope but here we will discuss only 2 types.
Transmission electron microscopy is a microscopy technique in which a beam of electrons is transmitted through an ultra-thin specimen, interacting with the specimen as it passes through it. Its resolution & magnification is about 10,000,000x. There are 5 types of transmission electron microscope i.e. BFTEM (Bright field transmision electron microscope), DFTEM (Dark field transmission electron microscope), HRTEM (High resolution transmission electron microscope), EFTEM (Energy filtered transmission electron microscope), ED (Electron diffraction). there are 4 techniques of TEM i.e. negative staining, shadow casting, Freeze fracture replication, freeze etching. It has many applications e.g, for the study of Cancer research, virology, chemical industry, electronic structure etc.
A scanning electron microscope is a type of electron microscope that produces images of a sample by scanning it with a focused beam of electrons. Types of signals produce by SEM include secondary electrons, back scattered electrons, X-rays, light rays. There are many advantages of SEM e.g, Btter resolution, fast imaging easy to operate, work with low voltage etc.
Transmission Electron Microscope (TEM), RESOLVING POWER, Scanning Electron Microscope, PRINCIPLE AND WORKING OF SEM, SEM SAMPLE PREPARATION, Limitations of Scanning Electron Microscopy (SEM), ADVANTAGES & DISADVANTAGES OF SEM, APPLICATIONS OF SEM, PRINCIPLE, AND WORKING OF TEM, SAMPLE PREPARATION FOR TEM, ADVANTAGES & DISADVANTAGES OF TEM, APPLICATIONS OF TEM, Differences between SEM and TEM.
scanning electron microscope for analysisM Ali Mohsin
SEM stands for scanning electron microscope. The SEM is a microscope that uses electrons instead of light to form an image. Since their development in the early 1950's, scanning electron microscopes have developed new areas of study in the medical and physical science communities.
Overview of the fundamental roles in Hydropower generation and the components involved in wider Electrical Engineering.
This paper presents the design and construction of hydroelectric dams from the hydrologist’s survey of the valley before construction, all aspects and involved disciplines, fluid dynamics, structural engineering, generation and mains frequency regulation to the very transmission of power through the network in the United Kingdom.
Author: Robbie Edward Sayers
Collaborators and co editors: Charlie Sims and Connor Healey.
(C) 2024 Robbie E. Sayers
Water scarcity is the lack of fresh water resources to meet the standard water demand. There are two type of water scarcity. One is physical. The other is economic water scarcity.
Welcome to WIPAC Monthly the magazine brought to you by the LinkedIn Group Water Industry Process Automation & Control.
In this month's edition, along with this month's industry news to celebrate the 13 years since the group was created we have articles including
A case study of the used of Advanced Process Control at the Wastewater Treatment works at Lleida in Spain
A look back on an article on smart wastewater networks in order to see how the industry has measured up in the interim around the adoption of Digital Transformation in the Water Industry.
COLLEGE BUS MANAGEMENT SYSTEM PROJECT REPORT.pdfKamal Acharya
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Immunizing Image Classifiers Against Localized Adversary Attacksgerogepatton
This paper addresses the vulnerability of deep learning models, particularly convolutional neural networks
(CNN)s, to adversarial attacks and presents a proactive training technique designed to counter them. We
introduce a novel volumization algorithm, which transforms 2D images into 3D volumetric representations.
When combined with 3D convolution and deep curriculum learning optimization (CLO), itsignificantly improves
the immunity of models against localized universal attacks by up to 40%. We evaluate our proposed approach
using contemporary CNN architectures and the modified Canadian Institute for Advanced Research (CIFAR-10
and CIFAR-100) and ImageNet Large Scale Visual Recognition Challenge (ILSVRC12) datasets, showcasing
accuracy improvements over previous techniques. The results indicate that the combination of the volumetric
input and curriculum learning holds significant promise for mitigating adversarial attacks without necessitating
adversary training.
Saudi Arabia stands as a titan in the global energy landscape, renowned for its abundant oil and gas resources. It's the largest exporter of petroleum and holds some of the world's most significant reserves. Let's delve into the top 10 oil and gas projects shaping Saudi Arabia's energy future in 2024.
Final project report on grocery store management system..pdfKamal Acharya
In today’s fast-changing business environment, it’s extremely important to be able to respond to client needs in the most effective and timely manner. If your customers wish to see your business online and have instant access to your products or services.
Online Grocery Store is an e-commerce website, which retails various grocery products. This project allows viewing various products available enables registered users to purchase desired products instantly using Paytm, UPI payment processor (Instant Pay) and also can place order by using Cash on Delivery (Pay Later) option. This project provides an easy access to Administrators and Managers to view orders placed using Pay Later and Instant Pay options.
In order to develop an e-commerce website, a number of Technologies must be studied and understood. These include multi-tiered architecture, server and client-side scripting techniques, implementation technologies, programming language (such as PHP, HTML, CSS, JavaScript) and MySQL relational databases. This is a project with the objective to develop a basic website where a consumer is provided with a shopping cart website and also to know about the technologies used to develop such a website.
This document will discuss each of the underlying technologies to create and implement an e- commerce website.
Explore the innovative world of trenchless pipe repair with our comprehensive guide, "The Benefits and Techniques of Trenchless Pipe Repair." This document delves into the modern methods of repairing underground pipes without the need for extensive excavation, highlighting the numerous advantages and the latest techniques used in the industry.
Learn about the cost savings, reduced environmental impact, and minimal disruption associated with trenchless technology. Discover detailed explanations of popular techniques such as pipe bursting, cured-in-place pipe (CIPP) lining, and directional drilling. Understand how these methods can be applied to various types of infrastructure, from residential plumbing to large-scale municipal systems.
Ideal for homeowners, contractors, engineers, and anyone interested in modern plumbing solutions, this guide provides valuable insights into why trenchless pipe repair is becoming the preferred choice for pipe rehabilitation. Stay informed about the latest advancements and best practices in the field.
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Democratizing Fuzzing at Scale by Abhishek Aryaabh.arya
Presented at NUS: Fuzzing and Software Security Summer School 2024
This keynote talks about the democratization of fuzzing at scale, highlighting the collaboration between open source communities, academia, and industry to advance the field of fuzzing. It delves into the history of fuzzing, the development of scalable fuzzing platforms, and the empowerment of community-driven research. The talk will further discuss recent advancements leveraging AI/ML and offer insights into the future evolution of the fuzzing landscape.
2. Outline
• light microscope vs Electron microscope
• Types of Electron microscope
• Transmission electron micrscope
• Tem imaging
• Tem applications
• Advanateges
• Disadvantages
3. • Light
microscope
- simple to use
- Poor surface view
- Uses light rays to
illuminate specimens
Source
- Lenses are made of
glass
- Low magnification of
up to 1,500x
- Resolving power :-
200nm
• Electron microscope
- users require technical skills
- Good surface view and
internal detailnesses
- Uses a beam of electrons to
view specimens
- Lenses are made of
electromagnetic
- High magnification of up to
1,000,000x
- Resolving power :- .2 nm
- operates under a high
vacuum
4. Types of Electron microscope
• Scanning electron
microscope (SEM)
• Transmission electron
microscope(TEM )
5. TRANSMISSION ELECTRON
MICROSCOPE ( TEM )
• TEMs consist of the following components:
• An electron source
• Thermionic Gun
• Electron beam
• Electromagnetic lenses
• Vacuum chamber
• 2 Condensers
• Sample stage
• Phosphor or fluorescent screen
• Computer
6. TEM imaging
• A Transmission Electron Microscope produces a high-resolution,
black and white image from the interaction that takes place
between prepared samples and energetic electrons in the vacuum
chamber.
• During transmission, the speed of electrons directly correlates to
electron wavelength; the faster electrons move, the shorter
wavelength and the greater the quality and detail of the image.
7. TEM applications
• TEMs provide topographical, morphological,
compositional and crystalline information.
• This information is useful in the study of
crystals and metals, but also has industrial
applications
• TEMs can be used in semiconductor analysis
and production and the manufacturing of
computer and silicon chips.
8. Advantages
• TEMs have a wide-range of applications and can be utilized
in a variety of different scientific, educational and industrial
fields
• TEMs provide information on element and compound
structure
• Images are high-quality and detailed
• TEMs are able to yield information of surface features,
shape, size and structure
• They are easy to operate with proper training
9. Disadvantages
• TEMs are large and very expensive
• Laborious sample preparation
• Potential artifacts from sample preparation
• Operation and analysis requires special
training
• TEMs require special housing and
maintenance
• Images are black and white