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Splicing
The document discusses RNA splicing, focusing on the removal of introns and joining of exons through processes involving different classes of introns (group I and II) and the spliceosome complex. It highlights the roles of various RNA and protein components, including snRNPs, in the splicing mechanism which involves transesterification and the formation of intron lariats. Key splice sites and binding proteins are also mentioned in relation to the splicing process.
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Splicing
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- 2. EI GKM Splicing Splicing Remove of Intronand joining the exon together Three Classes of RNA Splicing Nuclear pre-mRNA Group I introns Group II introns EIE
- 3. GKM Splicing exon 3’ Group IIintrons Intron5’ exon GU5’ 3’AG OH P AOH Splice Site Splice Site branch site Transesterification which phosphodiester linkages within the pre-mRNA are broken and new ones are formed 2’ Nucleophile intron lariat
- 4. GKM Splicing exon 3’ Group Iintrons 5’ exon GU5’ 3’AG OH P GOH Splice Site Splice Site 3’
- 5. GKM Splicing Spliceosome 150 Proteins 5RNAs U1, U2, U4, U5, U6 100 -300 snRNP Small Nuclear Ribonuclear Proteins
- 6. GKM Splicing exon 3’ Spliceosome 5’ exonGU5’ 3’AG AOH 3’ Splice Site 5’ Splice Site branch site 2’ U2U1 U2AFBBp Branch point Binding Protein U4 U6 U5
- 7. GKM Splicing exon 3’ Spliceosome 5’ exon AOH2’ U2U4 U6 U5 exon 3’ AOH 2’ U2 U5
- 8. GKM Splicing 5’ exon U6 exon 3’ OH2’ U2 U5 Spliceosome A OH Intron lariat