This document discusses gel filtration chromatography, also known as size exclusion chromatography. It separates biomolecules based on hydrodynamic size. Larger molecules pass through the pores of the stationary phase more slowly than smaller molecules. Applications include estimating molecular weight, purifying protein mixtures, and changing solution conditions like pH or salt concentration. Common stationary phases include cross-linked agarose or dextran beads packed into a glass column.

1. Sharon Solomon

2.  SEC
 Gel-filtration (GFC)
 Gel-permeation (GPC)

3.  Liquid chromatography
 Column- microporous beads
 Particles with
◦ Greater volume- diffuse more- slower (too big-
excluded)
◦ Lesser volume- faster
 Volume & shape
 Colored markers estimate mass
 Animation

6.  Molecular weights
 Preparative- contamination analysis
 Separate mixture
 Disinfect protein solutions
 Fast seperation
 Change pH, salt concentration, buffer,

7.  Stationary phase can interact with particles
 Not very precise
 Gel medium
 Not for big volume of particles

8.  Glass Column- prepacked or empty-
Biosurplus, GE
 Gel Filtration Media- order from Biotech
suppliers such as Bridge Bioscience Corp,
Sytoplasm, Tosoh Bioscience Llc, GE, and Pall
Life Sciences

9. Applications: Principle and Applications of Size-Exclusion Chromatography. From
http://www.impactanalytical.com/applications/sec.aspx
Chromatography. From http://www.polymersolutions.com/secgpc.html
Gel Filtration media Suppliers. From http://www.biosciregister.com/Gel_Filtration_media/Suppliers/pid3943.htm
Gel filtration or gel exclusion chromatography. From http://www.ucl.ac.uk/~ucbcdab/enzpur/gelexcl.htm
Gel permeation chromatography. (n.d.). McGraw-Hill Dictionary of Scientific and Technical Terms. Retrieved October
03, 2008, from Answers.com Web site: http://www.answers.com/topic/gel-permeation-chromatography
Syafei, Arie Dipareza (2007). Gel Filtration Chromatography: protein fractionation. From
http://envirodiary.com/2007/04/13/gel-filtration-chromatography-protein-fractionation/
What is gel filtration and when is it used? From
https://www.genomiphi.com/aptrix/upp00919.nsf/content/669A0B7DE97A18AEC1256EB400483E8C?
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