The document discusses plant cell inclusions known as ergastic substances, which are non-living materials that arise from cellular metabolism and are classified into reserve, secretory, and excretory materials. It covers their properties, types, and significance in plant physiology and human welfare, including their roles in food storage, waste disposal, and production of important substances like enzymes and drugs. Overall, ergastic substances are essential for plant health and have substantial benefits for human civilization.

1. Welcome to
Plant Cell Inclusions: Ergastic substances, Hydathodes,
Cavities, Lithocysts & Laticifers
By
N.Sannigrahi, Associate Professor, Department of Botany,
Nistarini College, Purulia, 723101(W.B) India
The non-living cell inclusions produced as a result
of the metabolic activity of the cell are known as
ergastic substances or cell inclusions. These are
found in cytoplasm, vacuoles and cell walls and are
grouped into three broad categories-Reserve
materials, Secondary materials and Excretory
materials.

2. Site of cell inclusions

3. PROPERITIES OF CELL INCLUSIONS
• Cell inclusions are non-living substances present in
the plant cells
• They are called ergastic substances or ergastic bodies
• They may be present in soluble or insoluble state
either organic or inorganic in nature as told earlier
• They are present in components or sub-components
of cell
• They are the raw materials or products of cellular
metabolism
• They may be exported or expelled from the cell.

4. CLASSIFICATION
• Ergastic substances-either organic or inorganic in nature
present in cytoplasm or vacuole have wide importance like 1.
food products-starch & Aleuronic grains, 2. Waste products
like crystals, 3. other substances like rubber, mucilage, tannins,
latex, alkaloids etc are broadly classified under three
categories-
• A. Reserve materials-Carbohydrates, Nitrogenous materials ,
Fats & oils
• B. Secretory materials-Present in sacs or glands being
Enzymes, Nectar, coloring matter, vitamin, Hormones.
• C. Excretory materials-Metabolic by-products like alkaloids,
tannins, latex, mineral crystals including raphides, styloides,
prismatic crystals etc.

5. METABOLITES-TYPES
• PRIMARY METABOLITES
• Chlorophyll, Amino acids, Nucleotides, Simple-CHOs,
Membrane lipids
• Found throughout the plant kingdom
• Important roles in growth & development like
photosynthesis, respiration, solute transport, translocation,
nutrient assimilation, differentiation
• SECONDARY METABOLITES
• restricted distribution in plants particularly either in one
species or taxonomically related species extend
ecological, protective, pollination or agent for plant-plant
competition by giving color, pungencies & odors

6. RESERVE MATERIALS
• To make new protoplasm and cellular activities, autotrophs
stored reserve materials as Carbohydrates, Proteins, Oils &
fats
• CARBOHYDRATES:
• Monosaccharide-glucose(Grapes) or fructose(Homey0
• Oligosaccharides- Sucrose(Cane sugar) or Raffinose on
hydrolysis produce sugar
• Polysaccharides- Inulin ( Tuber of Dahlia, Helianthus)
• Starch-Insoluble polysaccharides in storage organs like
rhizomes, endosperm, corns, tubers, cotyledons like oryza,
Ipomoea, Avena
• Cellulose-Insoluble as in Spinach and leafy vegetables
• Pectin- Rich in galacturonic acid, rhamnose, arabinose and
galactose

7. PROTEINS & FATS
• PROTEINS
• Storage Proteins in aleurone grains, either amorphous
(Wheat endosperm)or crystalline (fruit of Capsicum),
bounded by protenaceous membranes, seeds , embryo,
endosperms.
• Protein matrix containing a crystalloid (outer layer of
endosperm of wheat)
• Protein matrix with globoid
• Protein matrix with crystalloid or globoid.
• OILS & FATS: Abundant in endosperm and perisperm of
plant parts produced by elaioplast or spherosomes,
volatile oil in Rose, wax, cutin & suberin as protective
layers.

8. SECRETORY MATERIALS
• Produced during plant metabolism & perform
specific role .Includes coloring matter, enzymes &
nectar
• Coloring matter- Xanthophylls imparts orange to
yellow color while anthocynin imparts red, blue,
violet or pink color , brings pollination , fruit
dispersal and others
• Enzymes- Catalyse, Lipase, protease, invertase &
Diastase
• Nectar- Nectaries consisting of glucose, fructose
& sucrose affect pollination by entomogenous
flowers

9. Nectar glands-secretion

10. EXCRETORY MATERIALS
• By-products of plant metabolism stored in dead cells,
disposed off via leaves, fruits, seeds, barks etc.They are-
• Alkaloids-Quinine in cinchona barks, morphine in Poppy
seeds,, atropine in Atropa belladona,
• Crystals-Crystal contains cells are called idioblasts
originate within vacuoles-
• Calcium oxalate crystal in Citrus, Vicia, Begonia ; sphere
crystal called sphaeraphide in Datura, Nerium etc
• Raphides-Needle shaped with pointed tips found in
Impatiens, Colocasia
• Styloides-elongated prismatic crystals in members of
Liliaceae & Agavaceae
• Rosette crystals-In the members of Apiaceae
• Hydathodes-Specialized pores present in the leaf margin
to exude drops of water( guttation) in some angiosperm
herbs like mustard. Grasses and members of
Saxifragraceae.

11. Hydathodes

12. RAPHIDES

13. PLANTS SOURCE: TAXONOMICALLY
• Crystals of Calcium carbonate –Cystolith found in parenchyma
, epidermal cells ( Lithocysts) in Ficus leaves, trichomes or
hairs( Humulus lupulus) leaves of Acanthaceae, Cucurbitaceae
& Urticaceae.The leaves of Mormordica contains double
cystolith, Calcium carbonate containing crystals appear grape
like clusters with a stalk attached to the cell wall from where it
hangs into the cell lumen, having large nucleus and dense
cytoplasm.
• Crystal Sand: Small prismatic crystals found in Atropa
belladona of Solanaceae.
• Essential oils-The odoriferous glands in flowers, leaves, fruits
as found in oil of Peppermint from Mentha piperita, clove oil
from Eugenia caryophylla, Eucalyptus oil from Eucalyptus
globoulus, attract vectors to bring pollination and dispersal of
seeds & fruits.

14. Image-Ergastic substances

15. CYSTOLITH

16. Glycosides, Gum, Latex-Source
• Glycosides-Composed of glucose and other substances like
sinigrin- sulpher containing glycoside in Brassica nigra,
digitogenin as in Digitalis purpurea.
• Gum-exudes from the barks of Acacia arabica, A.modesta,
cellulose of cell wall undergoes decomposition forms gum,
naturally but response to injuries to heal up the wounds, used
in medicine, confectionary, sizing and finishing materials in
industry(Sing & Jain, 1996)
• Latex- Special group of cells –laticifers present in the families
of Asclepiadaceae, Apocynaceae, Moraceae & Euphorbiaceae,
colorless, milky white-yellow or orange consists of
carbohydrates, alkaloids, organic acids, oils, resins, rubber,
styarch etc. Papain, an enzyme present in Carica papaya, latex
of opium rich in alkaloid, natural rubber from Hevea
brasiliensis

17. PRISMATIC CRYSTALS

18. ORGANIC ACIDS, RESINS, SILICA BODIES------
• Organic acids-during metabolism, leaves and fruits produced
different organic acids like tartaric acid in tamarind, malic acid
in apple, citric acid in citrus etc
• Resin-Solid or liquid, brittle in nature with complex chemical
composition, produced from secretory cells surrounding the
resin ducts; coniferous trees rich in resin, Canada balsam
obtained from conifer Abies balsama.
• Silica bodies-Composed of silicon dioxide found in the
epidermal cells of Poaceae & Cyperaceae
• Tannins-Non-nitrogenous phenol derivatives present in the cell
sap; yellow , red or brown in color appear granules in cell;
found in seed coats, in unripe fruits, leaves of the infected
tissues; present in the periderm & heart wood making hard and
impervious; used in medicine, leather, dye and other
industries.

19. CONCLUSION
• Thus, several chemical compounds occur as non-living
inclusions in the cytoplasm and these inclusions remain
dispersed either cell-sap or in cytoplasm. Apparently, the
ergastic substances seems to be appeared as not so important
as far as the structural attributes of the plants but they play also
vey important role for the welfare of the human civilization.
The different industrial products are derived using the ergastic
materials as the raw materials. A number of life saving drugs
are derived using this components as the welfare of the human
beings. This, the ergastic substances are very important to
plants for its morphological and physiological attributes and it
plays a very important role in the welfare of human
civilization.

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