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Seed Dormancy: Causes And Applicability
Pragya Naithani
45979
What is seed dormancy?
Seed dormancy is defined as a state in which
seeds are prevented from germinating even under
environmental conditions normally favorable for
germination.
These conditions are a complex combination of
water, light, temperature, gasses, hard seed coats, and
hormones.
S.No Types Causes Embryo character Pre-treatment
1 Physical Seedcoat impermeable
Fully developed, non-
dormant
Scarification (Mechanical and acid)
2 Physiological
Physiological inhibiting
mechanism of germination in
the embryo
Fully developed
dormant
Seed soaking in growth regulators
(GA3, Ethrel, and chemical solutions
(KNO3, Thiourea)
3 Combinations 1+2
Fully developed
dormant
Scarification followed by chemical
treatment
4 Morphological Under developed embryo
Under developed non-
dormant
Cold stratification
5
Morpho-
physiological
Under developed embryo,
physiological
Under developed
dormant
Stratification followed by chemical
soaking.
Comprehensive Classification of seed dormancy
Harper, 1957
Nature of origination Innate Induced Enforced
Time/Cause of origin Primary Secondary
Location of dormancy Exogenous Endogenous Combined
Most extensively used classification system of seed dormancy
Mechanism of Dormancy
Hormonal control theory.
Temperature and gaseous exchange restriction theory.
Metabolic control theory.
Hormonal control theory
Dormancy causing hormones are ABA andethylene.
Dormancy causing hormones and dormancy provoking
hormones (GA & Cytokinin) ratio determine the fate of
dormancy.
Chilling treatment, light exposure increases Gibberellin
level in seeds & causes germination.
HORMONAL MECHANISM:
ABA and GA have antagonistic roles in germination and
dormancy respectively.
ABA induces and maintains seed dormancy during embryo
maturation. The key to ABA metabolism are some specific genes
encoding key enzymes are of NCED family (NCED6 and
NCED9)
( Nine-cis-epoxy carotenoid dioxygenase)
Embryonic identity protein (FUS3), which positively
regulates ABAsynthesis.
Temperature and gaseous exchange theory
Restricted oxygen uptake (due to hard seed coat) and
high temperature induces dormancy in seeds.
Oxygen deficiency leads to inhibition of
Cytochrome oxidase activity which leads to
dormancy.
Metabolic control theory
Dormancy in seed is controlled by metabolic energy
produced in seeds.
Respiratory system blocked ATPsynthesis blocked increase in
AMP andADP leads to increase in the activity of Glycolytic
pathway.
Production of growth inhibitors at lower concentration of
oxygen.
Causes of Seed Dormancy
Hard seed coat
Immature embryo
Germination inhibitors
Dormancy due to specific light requirement.
Period after ripening
Temprature
Excessive Salts
Hard seed coat
19
• Water impermeability:
Leguminosae, Gramineae, Malvaceae,, Geraniaceae,
Ohenopodiaceae, Solanaceae, Chenopodiaceae,
Convolvulaceae, and Nymphaeaceae etc.
• Gasimpermeability :
Xanthium
• Mechanical resistance (physically prevents the expansion of the embryo)
: Alisma, Amaranthus, Capsella
Immature Embryo
• Rudimentary and poorly developed embryo :
• Embryo fully developed but unable to
resume growth :
22
Rudimentary and poorly developed embryo
The embryo does not develop as rapidly as surrounding tissues.
Embryos are still immature and rudimentary when the seeds are dispersed.
Anemone nemorosa,
Fraxinus,
Caltha palustris,
Ginkgo biloba,
members of Orchidaceae, Orobanchaceae etc.
Seeds need time for maturity
.
Embryo fully developed but unable to resume growth
Iris, cherry, tulip, poplar, apple, peach, pines, peas,
etc.
Seeds need Stratification or after- ripening.
Specific Light Requirement :
There two type of seeds for light sensitivity:
Positive photoblastic seeds: Remain dormant in the dark, germinate when exposed to light
Ficus, Lettuca sativa (requires red light (660nm) or white light)
Rhododendron, Tomato, Bignonia (requires a photoperiod of 12 or more hours)
 Negative photoblastic seeds: Remain dormant when exposed to light, germinate only in the
dark.
Tobacco, Amaranthus, Nigella, Phlox, Nemophila and Silene
The light sensitive seeds are called photoblastic.
Germination Inhibitors
Found in the pulp of the fruits, seed coat, endosperm and embryos or
structures surrounding them
Tomato fruit pulp – Caffeic acid and Ferulic acid
Avena sp.- glumes
Chemical substances
Organic Acids, Phenolics, Tannins, Alkaloids, Cyanides, Indoles, ABA,
Ammonia, Phthalides, Coumarin, Parascorbic Acid, Cyanides, Ferulic Acid,
Dinitrophenols,Aldehydes, Fluorides, Unsaturated Lactones
S.No Species Location of inhibitor Name of inhibitor
1 Gossypium spp. Pericarp, testa Absicic acid (ABA)
2 Coriandrum sativum Pericarp Coumarin
3 Helianthus annus Pericarp, testa Hydrocyanic acid
4 Oryza sativa Hull Probably ABA
5 Triticum spp. Pericarp, testa
Catechin, catechin tannins, several
unknowns
6 Hordeum vulgare Hull Coumarin, Phenolic acids. scopoletin
7 Elaegnus angustfolia Pericarp, testa Possibly coumarin
8 Beta vulgaris Pericarp
Phenolic acids, Possibly ABA, high
concentration of inorganic ions
9 Avena sativa Hull Unknown
Location and name of germination inhibitors in certain species
Top
Temperature
Some seeds require chilling treatment for germination.
Apple, Walnut, and Pinus (chilling temperature of 1 -5 ºC for few
weeks)
Dormancy factor DOG1
transcription levels are
enhanced by low temperatures
during seed maturation.
Excessive Salts
Seeds contain a high concentration of solutes which
do not allow the embryo to resume its growth
Atriplex
Osmotic stress
ApplicabilityofSeeddormancy
 Species survival after natural disasters, pandemic, abiotic
stress
 More time for widespread seed dispersal and escape from
unfavorable situations
 Hard seed coats allow seed to come out of the alimentary
canals of birds and other animals uninjured e.g., Guava
 Germination inhibitors present in the seed coats of
desert plants dissolves away after the rainfall. This
way dormancy ensures that seed gets proper supply
of water during its germination
 Facilitates conservation of wild, rare, endangered plant
species in seed bank / gene bank artificially for the
future use
 Allow storage of food grains for longer period of time
Groundnut:-
• Genetic manipulation of Spanish and Valencia bunch
varieties by introducing genes from the semi-
spreading and spreading varieties (Virginia types) for
seed dormancy of three to four weeks.
-Patro (2016)
OUAT,Oddisha
• Induction of Dormancy in Spanish Groundnut Seeds Using
low doses of Cobalt-60 Gamma Irradiation (50Gy) remain
dormant for five months after harvest
-Benslimani,N (2009)
Effect of environmental conditions on the dormancy and germination of
volunteer oilseed rape seed (Brassica napus)
Effect of time of incubation (5, 14, and 28 d), water potential (+ = 0 and -1,500 kPa), and
light conditions (FR and darkness) on induction of secondary dormancy in seed of two
cultivars of oilseed rape. Germination test at 12 C in darkness. (Data are logit transformed
values with percentage of dormant seed in parentheses)
Incubation time Potential = 0 kPa Potential =1,500 kPa
(d) Cultivar FR Dark FR Dark
5 Libravo - -1.09 (11.9) - -2.19 (0) -1.62 (4.7) - -1.88 (2.2)
Falcon - -1.87 (1.9) - -2.19 (0) -1.95 (1.1) -2.13 (0.3)
Mean - -1.48 (6.9) - -2.19 (0) -1.78 (2.9) -2.01 (1.21)
14 Libravo - -1.06 (12.8) - -2.19 (0) - -1.11 (10.8) - -1.41 (6.9)
Falcon - -0.95 (15.3) - -2.19 (0) - -1.68 (3.3) -2.01 (0.8)
Mean - 1.01 (14.1) - -2.19 (0) -1.40 (7.1) -1.71 (3.9)
28 Libravo ___b - -2.19 (0) -0.49 (26.7) -0.7 (20.3)
Falcon - - -2.19 (0) -0.95 (13.1) - -1.51 (5.0)
Mean - - -2.19 (0) -0.72 (19.9) -1.1 (12.6)
Francisca et al.,1998
Exploiting Weed Seed Dormancy for weed control
Soil fertility levels
• Sodium nitrite was more effective than nitrate in breaking dormancy of dehulled red
rice.
Cohn et al., 1983
• In wild oats, potassium nitrate stimulated germination of dormant seeds in the light.
Hilton et al., 1985
• Ammonium nitrate increased emergence of some moderately dormant ecotypes about
30% in pot experiments.
Agenbag et al., 1989
Light effects
• cultivation during daylight serves to increase weed populations
Wesson and Wareing, 1969
• Significantly less weed emergence takes place in plots cultivated at night than during
daylight, suggesting that this approach may have practical applications for weed
management
Hartmann and Nezadal
28

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Seed dormancy, its causes and applicability

  • 1. Seed Dormancy: Causes And Applicability Pragya Naithani 45979
  • 2. What is seed dormancy? Seed dormancy is defined as a state in which seeds are prevented from germinating even under environmental conditions normally favorable for germination. These conditions are a complex combination of water, light, temperature, gasses, hard seed coats, and hormones.
  • 3. S.No Types Causes Embryo character Pre-treatment 1 Physical Seedcoat impermeable Fully developed, non- dormant Scarification (Mechanical and acid) 2 Physiological Physiological inhibiting mechanism of germination in the embryo Fully developed dormant Seed soaking in growth regulators (GA3, Ethrel, and chemical solutions (KNO3, Thiourea) 3 Combinations 1+2 Fully developed dormant Scarification followed by chemical treatment 4 Morphological Under developed embryo Under developed non- dormant Cold stratification 5 Morpho- physiological Under developed embryo, physiological Under developed dormant Stratification followed by chemical soaking. Comprehensive Classification of seed dormancy
  • 4. Harper, 1957 Nature of origination Innate Induced Enforced Time/Cause of origin Primary Secondary Location of dormancy Exogenous Endogenous Combined Most extensively used classification system of seed dormancy
  • 5. Mechanism of Dormancy Hormonal control theory. Temperature and gaseous exchange restriction theory. Metabolic control theory.
  • 6. Hormonal control theory Dormancy causing hormones are ABA andethylene. Dormancy causing hormones and dormancy provoking hormones (GA & Cytokinin) ratio determine the fate of dormancy. Chilling treatment, light exposure increases Gibberellin level in seeds & causes germination.
  • 7. HORMONAL MECHANISM: ABA and GA have antagonistic roles in germination and dormancy respectively. ABA induces and maintains seed dormancy during embryo maturation. The key to ABA metabolism are some specific genes encoding key enzymes are of NCED family (NCED6 and NCED9) ( Nine-cis-epoxy carotenoid dioxygenase) Embryonic identity protein (FUS3), which positively regulates ABAsynthesis.
  • 8. Temperature and gaseous exchange theory Restricted oxygen uptake (due to hard seed coat) and high temperature induces dormancy in seeds. Oxygen deficiency leads to inhibition of Cytochrome oxidase activity which leads to dormancy.
  • 9. Metabolic control theory Dormancy in seed is controlled by metabolic energy produced in seeds. Respiratory system blocked ATPsynthesis blocked increase in AMP andADP leads to increase in the activity of Glycolytic pathway. Production of growth inhibitors at lower concentration of oxygen.
  • 10. Causes of Seed Dormancy Hard seed coat Immature embryo Germination inhibitors Dormancy due to specific light requirement. Period after ripening Temprature Excessive Salts
  • 11. Hard seed coat 19 • Water impermeability: Leguminosae, Gramineae, Malvaceae,, Geraniaceae, Ohenopodiaceae, Solanaceae, Chenopodiaceae, Convolvulaceae, and Nymphaeaceae etc. • Gasimpermeability : Xanthium • Mechanical resistance (physically prevents the expansion of the embryo) : Alisma, Amaranthus, Capsella
  • 12. Immature Embryo • Rudimentary and poorly developed embryo : • Embryo fully developed but unable to resume growth : 22
  • 13. Rudimentary and poorly developed embryo The embryo does not develop as rapidly as surrounding tissues. Embryos are still immature and rudimentary when the seeds are dispersed. Anemone nemorosa, Fraxinus, Caltha palustris, Ginkgo biloba, members of Orchidaceae, Orobanchaceae etc. Seeds need time for maturity .
  • 14. Embryo fully developed but unable to resume growth Iris, cherry, tulip, poplar, apple, peach, pines, peas, etc. Seeds need Stratification or after- ripening.
  • 15. Specific Light Requirement : There two type of seeds for light sensitivity: Positive photoblastic seeds: Remain dormant in the dark, germinate when exposed to light Ficus, Lettuca sativa (requires red light (660nm) or white light) Rhododendron, Tomato, Bignonia (requires a photoperiod of 12 or more hours)  Negative photoblastic seeds: Remain dormant when exposed to light, germinate only in the dark. Tobacco, Amaranthus, Nigella, Phlox, Nemophila and Silene The light sensitive seeds are called photoblastic.
  • 16.
  • 17. Germination Inhibitors Found in the pulp of the fruits, seed coat, endosperm and embryos or structures surrounding them Tomato fruit pulp – Caffeic acid and Ferulic acid Avena sp.- glumes Chemical substances Organic Acids, Phenolics, Tannins, Alkaloids, Cyanides, Indoles, ABA, Ammonia, Phthalides, Coumarin, Parascorbic Acid, Cyanides, Ferulic Acid, Dinitrophenols,Aldehydes, Fluorides, Unsaturated Lactones
  • 18. S.No Species Location of inhibitor Name of inhibitor 1 Gossypium spp. Pericarp, testa Absicic acid (ABA) 2 Coriandrum sativum Pericarp Coumarin 3 Helianthus annus Pericarp, testa Hydrocyanic acid 4 Oryza sativa Hull Probably ABA 5 Triticum spp. Pericarp, testa Catechin, catechin tannins, several unknowns 6 Hordeum vulgare Hull Coumarin, Phenolic acids. scopoletin 7 Elaegnus angustfolia Pericarp, testa Possibly coumarin 8 Beta vulgaris Pericarp Phenolic acids, Possibly ABA, high concentration of inorganic ions 9 Avena sativa Hull Unknown Location and name of germination inhibitors in certain species Top
  • 19. Temperature Some seeds require chilling treatment for germination. Apple, Walnut, and Pinus (chilling temperature of 1 -5 ºC for few weeks)
  • 20. Dormancy factor DOG1 transcription levels are enhanced by low temperatures during seed maturation.
  • 21. Excessive Salts Seeds contain a high concentration of solutes which do not allow the embryo to resume its growth Atriplex Osmotic stress
  • 22.
  • 23. ApplicabilityofSeeddormancy  Species survival after natural disasters, pandemic, abiotic stress  More time for widespread seed dispersal and escape from unfavorable situations  Hard seed coats allow seed to come out of the alimentary canals of birds and other animals uninjured e.g., Guava
  • 24.  Germination inhibitors present in the seed coats of desert plants dissolves away after the rainfall. This way dormancy ensures that seed gets proper supply of water during its germination  Facilitates conservation of wild, rare, endangered plant species in seed bank / gene bank artificially for the future use  Allow storage of food grains for longer period of time
  • 25. Groundnut:- • Genetic manipulation of Spanish and Valencia bunch varieties by introducing genes from the semi- spreading and spreading varieties (Virginia types) for seed dormancy of three to four weeks. -Patro (2016) OUAT,Oddisha • Induction of Dormancy in Spanish Groundnut Seeds Using low doses of Cobalt-60 Gamma Irradiation (50Gy) remain dormant for five months after harvest -Benslimani,N (2009)
  • 26. Effect of environmental conditions on the dormancy and germination of volunteer oilseed rape seed (Brassica napus) Effect of time of incubation (5, 14, and 28 d), water potential (+ = 0 and -1,500 kPa), and light conditions (FR and darkness) on induction of secondary dormancy in seed of two cultivars of oilseed rape. Germination test at 12 C in darkness. (Data are logit transformed values with percentage of dormant seed in parentheses) Incubation time Potential = 0 kPa Potential =1,500 kPa (d) Cultivar FR Dark FR Dark 5 Libravo - -1.09 (11.9) - -2.19 (0) -1.62 (4.7) - -1.88 (2.2) Falcon - -1.87 (1.9) - -2.19 (0) -1.95 (1.1) -2.13 (0.3) Mean - -1.48 (6.9) - -2.19 (0) -1.78 (2.9) -2.01 (1.21) 14 Libravo - -1.06 (12.8) - -2.19 (0) - -1.11 (10.8) - -1.41 (6.9) Falcon - -0.95 (15.3) - -2.19 (0) - -1.68 (3.3) -2.01 (0.8) Mean - 1.01 (14.1) - -2.19 (0) -1.40 (7.1) -1.71 (3.9) 28 Libravo ___b - -2.19 (0) -0.49 (26.7) -0.7 (20.3) Falcon - - -2.19 (0) -0.95 (13.1) - -1.51 (5.0) Mean - - -2.19 (0) -0.72 (19.9) -1.1 (12.6) Francisca et al.,1998
  • 27. Exploiting Weed Seed Dormancy for weed control Soil fertility levels • Sodium nitrite was more effective than nitrate in breaking dormancy of dehulled red rice. Cohn et al., 1983 • In wild oats, potassium nitrate stimulated germination of dormant seeds in the light. Hilton et al., 1985 • Ammonium nitrate increased emergence of some moderately dormant ecotypes about 30% in pot experiments. Agenbag et al., 1989 Light effects • cultivation during daylight serves to increase weed populations Wesson and Wareing, 1969 • Significantly less weed emergence takes place in plots cultivated at night than during daylight, suggesting that this approach may have practical applications for weed management Hartmann and Nezadal
  • 28. 28