Fungal secondary metabolites are organic compounds produced by fungi that are not essential for growth but provide benefits such as aiding competition, antagonism, and self-defense. The document discusses the importance of fungi in biotechnology and medicine, describing their use in producing antibiotics, enzymes, acids, and other pharmaceutical compounds. It outlines methods for isolating local fungi, testing their extracts against pathogens, optimizing production conditions, and characterizing purified compounds. The aim is to screen Iraqi fungi such as Rhizoctonia and Trichoderma for novel bioactive substances and examine their potential for medical applications.
secondary metabolites of plant by K. K. SAHU SirKAUSHAL SAHU
METABOLITES : Introduction . . .
The chemical compounds produced by plants are collectively called as phytochemicals.
Primary metabolites – participating in nutrition and metabolic processes inside the plant.
Secondary metabolites – those chemical compounds that do not participate in metabolism of plants but influencing the
ecological interactions between the plant and its environment.
The presentation gives overview of production of secondary metabolites using callus culture as well as tissue culture techniques. Various batch and continuous culturing process are described on the basis of secondary metabolite to be synthesised.
Organogenesis, in plant tissue cultureKAUSHAL SAHU
Introduction
Definition
Types of organogenesis
Organogenesis through callus formation (indirect organogenesis)
Growth regulators for indirect organogenesis
Organogenesis through adventitious organ (direct organogenesis)
Growth regulators for direct organogenesis
Factor affecting the soot bud differentiation
Organogenic differentiation
Application of organogenesis
Conclusion
References
secondary metabolites of plant by K. K. SAHU SirKAUSHAL SAHU
METABOLITES : Introduction . . .
The chemical compounds produced by plants are collectively called as phytochemicals.
Primary metabolites – participating in nutrition and metabolic processes inside the plant.
Secondary metabolites – those chemical compounds that do not participate in metabolism of plants but influencing the
ecological interactions between the plant and its environment.
The presentation gives overview of production of secondary metabolites using callus culture as well as tissue culture techniques. Various batch and continuous culturing process are described on the basis of secondary metabolite to be synthesised.
Organogenesis, in plant tissue cultureKAUSHAL SAHU
Introduction
Definition
Types of organogenesis
Organogenesis through callus formation (indirect organogenesis)
Growth regulators for indirect organogenesis
Organogenesis through adventitious organ (direct organogenesis)
Growth regulators for direct organogenesis
Factor affecting the soot bud differentiation
Organogenic differentiation
Application of organogenesis
Conclusion
References
Introduction :
Mycorrhizae are mutualistic symbiotic associations formed between the roots of higher plants and fungi.
Fungal roots were discovered by the German botanist A B Frank in the last century (1855) in forest trees such as pine.
In nature approximately 90% of plants are infected with mycorrhizae. 83% Dicots,79% Monocots and 100% Gymnosperms.
Convert insoluble form of phosphorous in soil into soluble form.
the presentation is about microbial endophytes, discovery of endophytes, their types, isolation methods of different types and identification and the useful impacts of them to the plant ecology.
Each and every organisms in this world has its significant role.What we have to do is just identify it intellectually.Fungi have unexpected remediation property.
Meristem tip culture for the production of the virus free plantsArjun Rayamajhi
This presentation gives general idea on the meristem tip culture for the production of the virus free plants. The principles, methods and procedures of the meristem tip culture included. General idea on different in vitro culture techniques for virus elimination meristem tip culture viz. thermotherapy, cryotherapy,chemotherapy and electrotherapy are provided.
Mycorrhiza Biofertilizer is also known as VAM (Myco = Fungal + rrhiza = roots) adheres to plants rhizoids leading to development of hyphae. Hyphae boost development and spreading of white root in to soil leading to significant increase in rhizosphere. These hyphae further penetrate and form arbuscules within the root cortical. VAM fungi form a special symbiotic relationship with roots of plant that can enhance growth and survivability of colonized plants. Mycorrhiza Biofertilizer is very useful in organic farming as well as normal commercial farming
The chemical compounds produced by plants are collectively referred to as phytochemicals. Biotechnologists have special interest in plant tissue culture for the large scale production of commercially important compounds. These include pharmaceuticals, flavours, fragrances, cosmetics, food additives, feed stocks and antimicrobials.
Most of these products are secondary metabolites— chemical compounds that do not participate in metabolism of plants. Thus, secondary metabolites are not directly needed by plants as they do not perform any physiological function (as is the case with primary metabolites such as amino acids, nucleic acids etc.). Although the native plants are capable of producing the secondary metabolites of commercial interest, tissue culture systems are preferred.
Introduction :
Mycorrhizae are mutualistic symbiotic associations formed between the roots of higher plants and fungi.
Fungal roots were discovered by the German botanist A B Frank in the last century (1855) in forest trees such as pine.
In nature approximately 90% of plants are infected with mycorrhizae. 83% Dicots,79% Monocots and 100% Gymnosperms.
Convert insoluble form of phosphorous in soil into soluble form.
the presentation is about microbial endophytes, discovery of endophytes, their types, isolation methods of different types and identification and the useful impacts of them to the plant ecology.
Each and every organisms in this world has its significant role.What we have to do is just identify it intellectually.Fungi have unexpected remediation property.
Meristem tip culture for the production of the virus free plantsArjun Rayamajhi
This presentation gives general idea on the meristem tip culture for the production of the virus free plants. The principles, methods and procedures of the meristem tip culture included. General idea on different in vitro culture techniques for virus elimination meristem tip culture viz. thermotherapy, cryotherapy,chemotherapy and electrotherapy are provided.
Mycorrhiza Biofertilizer is also known as VAM (Myco = Fungal + rrhiza = roots) adheres to plants rhizoids leading to development of hyphae. Hyphae boost development and spreading of white root in to soil leading to significant increase in rhizosphere. These hyphae further penetrate and form arbuscules within the root cortical. VAM fungi form a special symbiotic relationship with roots of plant that can enhance growth and survivability of colonized plants. Mycorrhiza Biofertilizer is very useful in organic farming as well as normal commercial farming
The chemical compounds produced by plants are collectively referred to as phytochemicals. Biotechnologists have special interest in plant tissue culture for the large scale production of commercially important compounds. These include pharmaceuticals, flavours, fragrances, cosmetics, food additives, feed stocks and antimicrobials.
Most of these products are secondary metabolites— chemical compounds that do not participate in metabolism of plants. Thus, secondary metabolites are not directly needed by plants as they do not perform any physiological function (as is the case with primary metabolites such as amino acids, nucleic acids etc.). Although the native plants are capable of producing the secondary metabolites of commercial interest, tissue culture systems are preferred.
“Isolation and Biochemical Characterization of Antibiotic Producing Microorga...IOSR Journals
The search for new antibiotics continues in a rather overlooked hunting ground. In the course of screening for new antibiotic-producing microorganisms, isolates showing antimicrobial activity were isolated from waste soil samples from various habitats in the Industrial Areas in Dheradun, Uttarakhand, India. Existing methods of screening for antibiotic producers together with some novel procedures were reviewed. Both modified agar-streak and agar-plug methods were used in the primary screens. The use of selective isolation media, with or without antibiotic incorporation and/or heat pretreatment, enhanced the development of certain actinomycete colonies on the isolation plates. Antibiotics have long been considered the “magic bullet” that would end infectious disease. Although they have improved the health of countless numbers of humans and animals, many antibiotics have also been losing their effectiveness since the beginning of the antibiotic era. Bacteria have adapted defenses against these antibiotics and continue to develop new resistances, even as we develop new antibiotics. In recent years, much attention has been given to the increase in antibiotic resistance. As more microbial species and strains become resistant, many diseases have become difficult to treat, a phenomenon frequently ascribed to both indiscriminate and inappropriate use of antibiotics in human medicine. However, the use of antibiotics and antimicrobials in raising food animals has also contributed significantly to the pool of antibiotic resistant organisms globally and antibiotic resistant bacteria are now found in large numbers in virtually every ecosystem on earth. Dual culture bioassays were used to screen seven selected Bacillus isolates for activity against four plant pathogenic fungi in vitro. All isolates were able to inhibit the pathogens to varying degrees. Two isolates, R29 and B81, were selected for further testing and characterization. Further bioassays were performed on five complex nutrient media which were adjusted to pH S.S and 7, and both incubated at 2SoC and 30°C" respectively. It was found that pH and media composition showed significant influences on the antifungal activities of the isolates tested, but that a SoC temperature difference in incubation temperature did not. Tryptone soy agar was found to give rise to the largest inhibition zones. Both isolates were tentatively identified using standard biochemical and morphological tests. Based on its phenotypic characteristics, R29 was identified as a strain of B. subtilis. B81 proved to be more difficult to assign to a specific group or species of Bacillus, though B. subtilis and B. licheniformis were considered to be the nearest candidates. Genomic DNA was extracted from both isolates and a portion of each of their 16s rDNA genes were amplified and sequenced for homology testing against the GeneBank database. Homology testing confirmed that both isolates were members of the genus Bacillus and most
Evaluation of the Antibacterial Activities of Crude Extracts of Cochlospermum...iosrjce
The antibacterial activities of the methanolic, hot water, chloroform and petroleum ether extracts of
Choclospermum planchonii leaf were tested against some clinical bacterial isolates and reference organisms.
The phytochemical screenings of the extracts were also carried out. The leaf extracts of the plant exhibited
antibacterial activities against clinical isolates of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus
aureus, Shigella flexneri, and Salmonella typhii and reference strains of Staphylococcus aureus (ATCC 28923),
Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853. The methanolic leaf extracts
exhibited the highest antibacterial activity, its minimum inhibitory concentration (MIC) ranging between 0.31
and 1.25 mg/ml; and its zones of inhibition on the various test microorganisms ranging between 8mm and 13
mm. The petroleum ether extracts had the weakest antibacterial activity, its minimum inhibitory concentration
(MIC) ranging between 2.50mg/ml and 5.00mg/ml and its inhibition zone ranging between 4mm and 8mm. The
phytochemical constituents present in the plant were alkaloids, tannins, saponins, cardiac glycosides, and
sterols. The methanolic extracts of the leaf exhibited potentially biological activity than other extracts. This
indicates that the plant leaf could be useful in the treatment of human infections caused by these pathogens. The
antibacterial activities of the methanolic leaf extract may be attributed to the presence of bioactive compounds.
Bioactivity screening of Soil bacteria against human pathogenspharmaindexing
Microorganisms have a profound effect on medical science as they not only infect & cause disease but also produce metabolic products that can cure infections. Soil happens to be a source for a variety of microorganisms. Most of the bacteria, particularly actinomycetes produce biologically active secondary metabolites. Though there are a number of antibiotics available, there is a pressing need for the discovery of new source for antimicrobials against the pathogens due to the development of drug resistance of the pathogenic microorganisms. In addition to, new pathogenic strains are also developing and causing infection to human beings. Bioactive compounds are compounds that are produced by any living organism and are known to exhibit various biological activities both in-vitro & in-vivo. Bioactivity may be antimicrobial, antineoplastic, anticancerous, immunomodulation, antifertility & others. Soil bacteria were isolated by standard technique and by making use of selective media. The isolates were identified and subjected for preliminary screening to look for their ability to produce bioactive materials. A total of 96 strains were isolated from three different soil samples. 14 of them were found to have antibacterial activity against the human pathogens like Staphylococcus aureus, Streptococcus faecalis, E.coli, Klebsiella aerogenes, Proteus vulgaris, Pseudomonas aureginosa and Salmonella typhi by preliminary screening. Further the selected (3) bacteria were grown in the suitable culture media for the production of bioactive metabolites by using rotary shake flask. The active metabolites was isolated by solvent extraction and concentrated by evaporation under reduced pressure. The antimicrobial screening of the active metabolites showed prominent effect against the clinical pathogens under the study.
A Strategic Approach: GenAI in EducationPeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
A workshop hosted by the South African Journal of Science aimed at postgraduate students and early career researchers with little or no experience in writing and publishing journal articles.
Introduction to AI for Nonprofits with Tapp NetworkTechSoup
Dive into the world of AI! Experts Jon Hill and Tareq Monaur will guide you through AI's role in enhancing nonprofit websites and basic marketing strategies, making it easy to understand and apply.
This presentation includes basic of PCOS their pathology and treatment and also Ayurveda correlation of PCOS and Ayurvedic line of treatment mentioned in classics.
MATATAG CURRICULUM: ASSESSING THE READINESS OF ELEM. PUBLIC SCHOOL TEACHERS I...NelTorrente
In this research, it concludes that while the readiness of teachers in Caloocan City to implement the MATATAG Curriculum is generally positive, targeted efforts in professional development, resource distribution, support networks, and comprehensive preparation can address the existing gaps and ensure successful curriculum implementation.
The simplified electron and muon model, Oscillating Spacetime: The Foundation...RitikBhardwaj56
Discover the Simplified Electron and Muon Model: A New Wave-Based Approach to Understanding Particles delves into a groundbreaking theory that presents electrons and muons as rotating soliton waves within oscillating spacetime. Geared towards students, researchers, and science buffs, this book breaks down complex ideas into simple explanations. It covers topics such as electron waves, temporal dynamics, and the implications of this model on particle physics. With clear illustrations and easy-to-follow explanations, readers will gain a new outlook on the universe's fundamental nature.
2. The Fungi
Fungi are one of the largest group of
organisms , world wide distributed and
play a vital role in ecosystems and as one of
the most important tool in biotechnology
3. Fungi & Biotechnology
BIOTECHNOLOGY means “ Use of Fungi to
produce, develop, improve, remove or sustain of
a particular thing which can be useful for man
and nature “
The most important use of fungi is the
production of Secondary Metabolites
Such as Antimicrobial Bioactive Substances
that can be beneficial for Medical therapy
4. The economic significance of Fungi
Fermentation technology
Enzymes production technology
Production of acids and chemicals
Cultivation of fungi for protein
Food processing by fungi (Bread, cheese)
Fungi can be used in many applications in
(industry, agriculture, medicine , and
environment)
Production of Bioactive compounds (Antibiotics)
5. Secondary metabolites
Organic compounds , with low molecular weight
,which are not essential for fungal growth but
their natural production have certain
significances.
6. Why secondary metabolites are produced?
They are chemical compounds produced by many
fungi growing on substrates. They play a role in
competition ,antagonism and self-defense
mechanisms against other living organisms to
allow the fungus to occupy the niche and utilize
the food
7. Production of SECONDARY METABOLITES
Compounds produced during the stationary and
decline phases of the fungal growth
8.
They are vary in structures and biosynthetic
pathways . So they differ among the fungal
species and isolates
12. Antibiotics
Antibiotics: Are chemical substances produced by
fungi which has the capacity to inhibit the growth
of / and or even kill other microorganisms . The
action of an antibiotic is a selective in nature .
13. History of ANTIBIOTICS discovery
(1928) Alexander Flimmings discovered the
Penicillin
(1935) Prontosil, the first sulfa drug was
discovered by Gerhard Domagk
(1943) Andrew Moyer, industrial production of
Penicillin
(1943) Selman Waksman discovered the
Streptomycin from soil Bacteria
14. (1955) Tetracycline was patented by Lioyd Conover
(1957) Nystatin was patented
(1981) Smith Kline Beckham patented Amoxillin
Since then many antibiotics have been found and
still continued to discover more…….
15. Antibiotic can be divided according their
bioactivity into:
Antifungal antibiotics
Antibacterial antibiotics
Antiviral antibiotics
Antitumoral antibiotics
17. The aim of present study
1. Screening of local fungi isolates for antimicrobial bioactive
compounds production.
2. Isolation and purification of the bioactive compounds using
specific techniques
3. Examining the optimal conditions (Temp, pH, media , C, N
source and some other factors) for a mass production of these
compounds in batch cultures.
4. Characterization of the purified fungal extracts by using
HNMR and GC Mass.
5. Testing the inhibition activity of these compounds against a
selected pathogenic microorganisms (Bacteria, Candida,
Dermatophytes and other fungi).
18. Most recent publications on bioactive metabolites by fungi
Last decade several published papers on this aspect: For Examples
Anke et al. (2004, 2006) from Germany
Lindquist et al. (2005, 2006) from Germany
Abad et al. (2007) from Spain
Stamets P. (2007) from USA
Johnathan et al. (2010) South Africa
Muhsin et al (2011) from Iraq
Pohanka ( 2006 ) PhD. Thesis (Sweeden)
Roberts (2004). PhD. Thesis (Australia)
Khalaf KT (2008) PhD. Thesis (Iraq)
19. Materials and methods
1. Sample collection and fungal isolation:
Sample are collecting from different sources (soil , water , air ,
rhizospher , rhizoplane ) for fungal isolation
Isolation, purification and identification of fungi on solid
media
Screening of the inhibition bioactivity of each fungal species
against the selected pathogenic microorganisms including:
A. Bacteria B. Candida C. Dermatophytes D. Other fungi
by using fungal discs on solid media.
2. Fungal cultures Extraction:
The selected fungal isolates will be grown in specific liquid
culture
(using batch large bottles) under shaking condition for two week
incubation period at 25 C
20. 3. Antimicrobial bioactivity assay
Filter paper discs (0.6 mm) after being
sterilized by autoclave are socked in each
fungal crude extract solution for 5 min.,
Filter paper discs with extracts are placed on
the surface of agar medium in Petri-dishes
streaked with 0.2 ml of bacterial suspension
of Escherichia coli and Staphylococcus
aureus, Candida albicans, Microsporum
gypseum, Fusarium oxysporum.
Plates are incubated at 37 ◦C for 24 hr, an
appearance of inhibition zones around the
filter paper disc indicating the bioactivity of
crude metabolites of the tested fungal isolates
(Casals, 1979).
21. 4. Extraction of the fungal filtrates
Five discs (0.5 mm diam.) are cut from the axenic
fungal culture of each isolate by using a cork borer and
amended into PD liquid medium in 500 ml flasks (with
triplicates) and incubation at 25 ◦C for 2 weeks on a
rotary shaker.
Fungal cultures are filtered on Whatman No 1 filter
paper and the pH adjusted at 3 for each fungal filtrate.
Filtrate is extracted in ethyl acetate (1:1 vol) by using
separating funnel. The organic layer is collected by
dehydration of water by using Na2SO4. The filtrate is
filtered again and place in Petri dishes then leave to be
dried at room temperature.
100 ug of the dried extract is dissolved in 1 ml ethanol
as stock extract solution to be used for further
experiments.
22. 5. Minimal inhibitory concentration test
The MIC values will be determined by the standard
serial dilution assay (McGinnis, 198o).
Different concentrations of the crude fungal extract
are used. Filter paper discs (0.5 mm) are soaked
in each extract concentration and will be tested
against each selected microorganisms on solid
medium. The appearance of inhibition zone
around the disc indicates an inhibitory action.
23. 6.Cytotoxicity test
Cytotoxicity of the fungal crud extracts was examined
by using human RBC
following a previously described method ( Xian- guo
and Ursula, 1994).
7. Chemical analysis of fungal crude extracts
Fungal culture extracts will be chemically analyzed for
alkaloids, phenols, amino acids, flavenoides and
Tannins according to following the described method
8. Purification of fungal extracts
Crud extract will purified by using TLC and HPLC
technique.
9.Identification and characterization of the
purified extract
By using GC –Mass and HNMR technique
24. 10.Optimal Production of Bioactive metabolites
Experimentation will be conducted to examine the
optimal production of the bioactive compounds in
batch liquid cultures using:
1. Effect of Temperature
2. Effect of pH
3. Effect of Media
4. Effect of carbon and nitrogen sources (4 sources)
5. Effect light and dark period
6. Effect microelements (eg Zn, Fe)
11. Comparison of the bioactivity of the studied
compounds with some commercial antibiotics.
12. If the allows, some other works will be included
25. Selected fungi for this study
We try to investigate some fungi
belong to Deuteromycetes in particular
Rhizoctonia and Trichoderma
For the following reasons:
1. No research so far dealt with these
fungi elsewhere
2. These fungi are highly competitive
against other soil and plant fungi
3. Easy to grow in culture and handle
with