Dr Evgeny Sagulenko's Biofertilizer Technology Increases Crop Yields
1. Dr Evgeny Sagulenko
Director (Research and Development, SOS)
Education – MS, biology, St Petersburg State University, Russia;
- PhD, microbiology and genetics, All-Russia Institute for
Agricultural Microbiology, St Petersburg, Russia
Experience – microbiology (>20 years) – Russia, USA, Australia
genetics and cell biology (>20 years) – Russia, USA, Germany, Australia
cancer research (5 years) - Germany
Research interests – agricultural microbiology
- general microbiology
- agriculture
Publications – more than 20 papers published in peer-reviewed journals (h-factor 13)
http://scholar.google.com/citations?user=xRnMDKYAAAAJ&hl=en
2. Patent: Application of bacteria of the genus Burkholderia for
agricultural practices
New biofertilizer – great opportunity to
change agriculture management
6. Extensive use of
inorganic fertilizers
Salt accumulation
Water pollution
Plant damage
Damage of soil structure
Higher crop yield requires extensive use of fertilizers
7. Photo copyright J Henry Fair industrialscars.com - See more at:
http://www.inspirationgreen.com/chemical-fertilizers.html#sthash.H2q7lHWK.dpuf
Early morning fertilizer runoff after late night storm.
8. Global fertilizer use
In million tons...
- See more at: http://www.inspirationgreen.com/chemical-fertilizers.html#sthash.H2q7lHWK.dpuf
9. Excess of nitrates
Nitrate is a potential health hazard.
It may increase a risk of:
• Methemoglobinemia or “blue baby syndrome” which results from nitrate
decreasing the blood’s capacity to carry oxygen, especially in infants who receive baby
formula mixed with water containing nitrate above 10 mg/L
• Recurrent respiratory infections
• Thyroid dysfunction
• Negative reproductive outcomes
such as spontaneous abortion
• Certain cancers including
cancer of the stomach or bladder
• A food allergy occurs when your body perceives a substance such as
nitrates or nitrites as foreign substances. http://www.livestrong.com/article/472610-nitrite-nitrate-allergies/
10. China is the world's top consumer of fertilizers and it is estimated that 10 million tons of
fertilizer end up in China's waterways.
Such is the case in China, where acidic soils boost parasite
abundance and facilitate the leaching of toxic metals like
aluminum and manganese. There, agricultural experts say
that many areas would benefit from application reductions
of up to 60%.
Negative impact of inorganic fertilizers
11. Sea and land pollution
Dark red circles indicate oil spills and gray-shaded areas indicate sea pollution and land pollution from chemical
fertilizers. worldprocessor.com - See more at: http://www.inspirationgreen.com/chemical-
fertilizers.html#sthash.H2q7lHWK.dpuf
12. Future direction – introduction of new sustainable practices in agriculture
UNESCO - holistic approach to agriculture would recognise the linkages between
the soil, vegetation, air and water
14. Bacteria of the genus Burkholderia are able to increase plant growth
Abstract
Send to:
Microb Biotechnol. 2014 Mar;7(2):142-54. doi: 10.1111/1751-7915.12105. Epub 2013 Dec 19.
A new species of Burkholderia isolated from sugarcane roots promotes plant growth.
Paungfoo-Lonhienne C1, Lonhienne TG, Yeoh YK, Webb RI, Lakshmanan P, Chan CX, Lim PE, Ragan MA, Schmidt S,
Hugenholtz P.
Major publication:
15. How bacteria help plants to grow
Similar bacterium - Rhizobium forms nodules on plant roots (fabaceae – legumes, beens)
Fix nitrogen,
obtain carbon
and phosphorus
from plants !
17. N2
P, C, S, N, microelements
from organic sources
How our technology works
Bacteria
18. Fix nitrogen
from the air,
obtain carbon
and phosphorus
from fertilizers
(organic/inorganic)
Burkholderia are doing similar job to Rhizobium, but instead of nodules they
create biofilm on plant roots
Burkholderia
19. Organic fertilizers
Slow uptake of nutrients
Inorganic fertilizers
Fast uptake of nutrients
Organic fertilizers plus bacteria
Fast uptake of nutrients
Our technology
24. Phytate
Phytate is highly abundant in manure!
Lott et al. (2000) estimated that 51 million tonns of phytate is sequestered annually in
commercially produced crop seeds and fruits.
25. Cost of phytate in China
Low Price phytic acid 70%,food grade competitive price
US $0.02-5000 / Tonne ( FOB Price)
1 Ton (Min. Order)
Type: Acidity Regulators,Antioxidants,Chewing Gum Bases,Enzyme Preparations,Flavoring Agents,
Nutrition Enhancers,Thickeners
Place of Origin: CC
Model Number: phytic acid
China (Mainland) Contact Details
Trade Assurance
>72h Average Response Time
Contact Supplier
Leave Messages
High Quality 50%-70% Fytic Acid Phytic Acid Price
US $0.02-5000 / Tonne ( FOB Price)
1 Ton (Min. Order)
Type: Acidity Regulators,Antioxidants,Chewing Gum Bases,
Enzyme Preparations,Flavoring Agents,
Nutrition Enhancers,Thickeners
Place of Origin: CC
Model Number: phytic acid
Tags: High Quality 50%-70% Fytic Acid Phytic Acid Price
http://www.alibaba.com/showroom/phytic-acid-price.html
28. Crops grown with our technology will be richer in microelements
(more tasty too)
29. Application of the technology for hydroponics
In traditional hydroponics, organic
fertilizer cannot be used because its
base compounds natural
hydroponics inhibit the growth of
the roots.
31. Application of the technology for green house
Manure or phytate
bacteria Burkholderia
32. Application of the biofertilizer to the open fields
Granules containing
organic fertilizer/phytate and
bacteria Burkholderia
33. Crop 1970 1980 1990 2000 2010
Tomatoes 35,884,500 52,650,400 76,309,700 110,391,000 152,008,000
World: production of crops in metric tonnes
Major crops to be tested for industrial-scale production
(hydroponics, greenhouse, open field)
China is producing 30,000,000 tons (faostat via growtomatoes.com)
A potential increase of 30% with our technology
(30,000,000 tons at a value of US$76 per ton in returns to producers is US$ 2.8 billion
34. Crops 1970 1980 1990 2000 2010 2013
Rice 316,346,000 396,871,000 518,569,000 599,355,000 701,999,000 745,710,000
Wheat 310,741,000 440,188,000 592,311,000 585,691,000 649,325,000 713,183,000
World: production of crops in metric tonnes
Major crops to be tested for industrial-scale production
(hydroponics, greenhouse, open field)
35. China produces 141,500,000 tons (USDA)
A potential increase in returns to producers
is US$ 59.5 billion - with an increase of
30% with our technology (223,500,000 tons
at a value of US$254 per ton (USDA)
Rice
China produces 121,000,000 tons (USDA)
(faostat via growtomatoes.com)
A potential increase in returns to producers
is US$ 55.6 billion - with an increase of
30% with our technology (235,800,000 tons
at a value of US$236 per ton (USDA)
Wheat
36. Crops 1970 1980 1990 2000 2010 2013
Maize 265,831,000 396,623,000 483,373,000 592,479,000 851,271,000 1,016,740,000
Lettuce 6,563,050 8,449,160 11,572,500 18,269,800 24,352,200 ..
World: production of crops in metric tonnes
Major crops to be tested for industrial-scale production
(hydroponics, greenhouse, open field)
37. Maize
China produces 240,000,000 tons (Faostat)
A potential increase in returns to producers
is US$ 50.01 billion - with an increase of
30% with our technology (684,000,000 tons
at a value of US$149 per ton (USDA)
39. Technology current status
• Fundamentals of technology are well understood
– Bacteria are isolated from the sugar cane roots by technology developers
– Bacteria metabolism is under investigation
• Laboratory trials have been conducted
– Proven root and plant growth rates increase for the following plants:
» Rice
» Tomato
» Wheat
» Corn
40. Plant growth without and with the biofertilizer
Rice
Without biofertilizer With biofertilizer
42. Technology development costs
• Commercialisation of this technology requires ~3,000,000 USD
• Costs include:
– Biofertilizer technology development
– Hydroponics, greenhouse and field trials
– Market studies
– Commercialisation
43. Technology development roadmap
Bacteria
preparati
on for
trials
Additional bacteria isolation and characterisation and understanding
a potential for further improvements
Bacteria fertiliser
optimisation
Hydroponic and
greenhouse trials
(tomato, lettuce)
Field trials (tomato, rice, wheat)
Commercialisation of hydroponic
and greenhouse applications
Months
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
Commercialisation of field
crops applications
Estimation of selling price,
based on the technology
performance
Estimation of selling price,
based on the technology
performance
46. Summary
Accumulation of nitrates, phosphates within plant tissues
Crop yield
Crop quality
Environmental impact
Cost of agricultural production
Benefits of our technology
Entrepreneurs See A New Field Of Dreams
In Agriculture
47. Yet there’s another market so big it leaves mobile phones in the dust. The
world’s farmers will need to feed 9.6 billion people by 2050, the United Nations
says, and that will require a 70% increase in agricultural production.
Agriculture is a $3 trillion industry increasingly driven by technology
developments, but until recently it was practically ignored by venture
capitalists.
http://cen.acs.org/articles/93/i19/Entrepreneurs-See-New-Field-Dreams.html
