The document presents information on bacterial diseases in fish and shrimp. It discusses 10 common bacterial diseases that affect fish, including furunculosis, columnaris disease, vibriosis, fin and tail rot disease, dropsy, cotton mouth disease, tuberculosis disease, bacterial gill disease, edwardsiellosis, and pseudomonasis. It provides details on the causal agents and susceptible species for each disease. It also discusses 3 main bacterial diseases that affect shrimp: rickettsial infections, vibriosis, and brown spot shell disease. The document emphasizes the importance of water quality, sanitation, and nutrition in preventing outbreaks of bacterial disease.
cultured shrimp are getting affected by various disease.some of them are acute and some chronic. and the curing is very harder for a farmer so it is better suggested for safety precaution and proper hygiene while culturing.and the affected shrimp in cured with antibiotics is not accepted by anyone in the export business. so, let yourself find out the various shrimp disease their cure and proper management in this seminar.
cultured shrimp are getting affected by various disease.some of them are acute and some chronic. and the curing is very harder for a farmer so it is better suggested for safety precaution and proper hygiene while culturing.and the affected shrimp in cured with antibiotics is not accepted by anyone in the export business. so, let yourself find out the various shrimp disease their cure and proper management in this seminar.
Introduction
Fish Health Management GOALS
Principles of fish health management
Factors affecting fish health
Common symptoms of diseases
General preventive measures
Proper Health Management through Manipulating the disease triangle
Conclusion
References
Carps form the mainstay of aquaculture in India contributing over 85% of the total aquaculture production There are 61,259 species of vertebrates recognized world; over 30,700 are fish species of which 8,411 ore fresh water while 11,650 are marine. In India 2,163 spp. are fin fishes have been recorded from upland cold water (157; 7.26%) warm water of the plain (54; 20.99%), Brackish water (182; 8.41%) and marine environment (1,370; 63.43%). Some of these species are cultured at commercial level which covering a lot varieties of fin fishes The three Indian major carps, namely Catla (Catla catla), Rohu (Labeo rohita) and Mrigal (Cirrhinus mrigal) contribute the bulk of production to the extent of 75 to85 percent of the total fresh water fish production, the three exotic carp such as Silver carp (Hypophthalmichthys molitrix) and Common carp (Cyprinus carpio ), Grass carp (Ctenopharyngodon idella )form the second important group to incorporate several other medium and minor carp and into the carp poly culture system several method were used because of their region specific consumer preference and higher market demand.
History
Carp culture in India was restricted only to a homestead backyard pond activity in west Bengal and Odisha until late 1950 s with seed from riverine sources as the only input resulting low level of production the technological breakthrough breeding of carp through hypophysation in 1957 freshwater aquaculture of the country the country till 1984 virtually laid the foundation of scientific carp farming in the country.
Important characteristics of Indian major carps:-
Indian major carp grow fast and can reproduce even in artificial ponds. They feed upon phytoplankton, zooplankton, decaying organic matter, aquatic plant etc. stomach is absent in the alimentary canal of the major carps. Three types of Indian major carps are cultured in
.DEFINITION OF FISH PHARMACOLOGY:
“Fish pharmacology is essential for undertaking treatment of fishes using any therapeutic chemicals or drugs.”
“Pharmacology is the study of the interaction of chemicals with living system.”
“Pharmacology” is morden science which correlated other biological sciences, eg., Biochemistry , Physiology Microbiology , Medicine , and Genetics.
“Pharmacology is the branch of biology concerned with the study of drug action.”
“Pharmacology is the study of drugs including their origins, history, uses, and properties. It mainly focuses on the actions of drugs on the body.”
“Pharmacology is the study of drugs and theire actions on the body”.
“Pharmacology is the study of substance that interact with living systems through chemical process, especially by binding to regulatory molecules &activating or inhibiting normal body process.
Fig. 1
3. HISTORY OF FISH PHARMACOLGY:
Pharmacology emerged as its own discipline in the 19th Century, branching off from research done in fields of science such as organic chemistry and physiology. Oswald Schmiedeberg, who was born in what is now Latvia in 1838, is considered the father of pharmacology. His doctoral thesis was on the measurement of chloroform levels in blood, and he went on to become a professor of pharmacology at the University of Strasburg, where he ran an institute of pharmacology. There, he studied chloroform, which was used as an anesthetic, chloral hydrate, a sedative and hypnotic, and muscarine, a compound isolated from the mushroom Amanita muscaria that stimulates the parasympathetic nervous system and has been used to treat various diseases such as glaucoma.
In 1890, John Jacob Abel became the first pharmacology chair in the United States, at the University of Michigan. He later went to Johns Hopkins University in Baltimore. Abel was the first to isolate the hormone epinephrine from the adrenal gland, isolate histamine from the pituitary gland, and make pure crystalline insulin. Animals such as dogs, cats, pigeons, and frogs were used to test pharmacological substances. Humans were even used as test subjects. Sometimes they suffered through severe adverse effects from these substances, such as when the German pharmacist Friedrich Serturner and three of his friends had poisoning for several days from an alkaloid that Serturner had isolated from opium. This alkaloid was later named morphine, after the Ancient Greek god of sleep, Morpheus.
Oswald Schmiedeberg was a brilliant scientist. He studied the pharmacology of various compounds, including chloroform, and published an important text called the Outline of Pharmacology. There, he studied chloroform, which was used as an anesthetic, chloral hydrate, a sedative and hypnotic, and muscarine, a compound isolated from the mush to the field, Schmiedeberg is now known as 'the father of pharmacology'.
Non-Infectious Disease
Not caused by pathogens
Cannot be transmitted to other species
Malnutrition, Avitaminoses, Heavy Metals etc. are responsible
Risk factors:
Genetics
Life-style
Environmental factors
Genetic Risk Factors
Determined by genes
Familial Disease Tendency
Disease runs in species
Recessive gene disorders
Down syndrome
Born with extra chromosome
Sex-linked disorders
Linked to x chromosome (female)
Can be recessive in females
Color blindness, hemophilia, & muscular dystrophy
A brief presentation on fish vaccination and its application particularly in Bangladesh. The overall process is described in a nutshell here. The types, procedure of formation, regulation, licensing and use are among them.
The measures and methods adopted to secure a disease free environment in all phases of aquaculture practices (i.e. hatcheries, nurseries, grow-out farms) for improved profitability.
Introduction
Fish Health Management GOALS
Principles of fish health management
Factors affecting fish health
Common symptoms of diseases
General preventive measures
Proper Health Management through Manipulating the disease triangle
Conclusion
References
Carps form the mainstay of aquaculture in India contributing over 85% of the total aquaculture production There are 61,259 species of vertebrates recognized world; over 30,700 are fish species of which 8,411 ore fresh water while 11,650 are marine. In India 2,163 spp. are fin fishes have been recorded from upland cold water (157; 7.26%) warm water of the plain (54; 20.99%), Brackish water (182; 8.41%) and marine environment (1,370; 63.43%). Some of these species are cultured at commercial level which covering a lot varieties of fin fishes The three Indian major carps, namely Catla (Catla catla), Rohu (Labeo rohita) and Mrigal (Cirrhinus mrigal) contribute the bulk of production to the extent of 75 to85 percent of the total fresh water fish production, the three exotic carp such as Silver carp (Hypophthalmichthys molitrix) and Common carp (Cyprinus carpio ), Grass carp (Ctenopharyngodon idella )form the second important group to incorporate several other medium and minor carp and into the carp poly culture system several method were used because of their region specific consumer preference and higher market demand.
History
Carp culture in India was restricted only to a homestead backyard pond activity in west Bengal and Odisha until late 1950 s with seed from riverine sources as the only input resulting low level of production the technological breakthrough breeding of carp through hypophysation in 1957 freshwater aquaculture of the country the country till 1984 virtually laid the foundation of scientific carp farming in the country.
Important characteristics of Indian major carps:-
Indian major carp grow fast and can reproduce even in artificial ponds. They feed upon phytoplankton, zooplankton, decaying organic matter, aquatic plant etc. stomach is absent in the alimentary canal of the major carps. Three types of Indian major carps are cultured in
.DEFINITION OF FISH PHARMACOLOGY:
“Fish pharmacology is essential for undertaking treatment of fishes using any therapeutic chemicals or drugs.”
“Pharmacology is the study of the interaction of chemicals with living system.”
“Pharmacology” is morden science which correlated other biological sciences, eg., Biochemistry , Physiology Microbiology , Medicine , and Genetics.
“Pharmacology is the branch of biology concerned with the study of drug action.”
“Pharmacology is the study of drugs including their origins, history, uses, and properties. It mainly focuses on the actions of drugs on the body.”
“Pharmacology is the study of drugs and theire actions on the body”.
“Pharmacology is the study of substance that interact with living systems through chemical process, especially by binding to regulatory molecules &activating or inhibiting normal body process.
Fig. 1
3. HISTORY OF FISH PHARMACOLGY:
Pharmacology emerged as its own discipline in the 19th Century, branching off from research done in fields of science such as organic chemistry and physiology. Oswald Schmiedeberg, who was born in what is now Latvia in 1838, is considered the father of pharmacology. His doctoral thesis was on the measurement of chloroform levels in blood, and he went on to become a professor of pharmacology at the University of Strasburg, where he ran an institute of pharmacology. There, he studied chloroform, which was used as an anesthetic, chloral hydrate, a sedative and hypnotic, and muscarine, a compound isolated from the mushroom Amanita muscaria that stimulates the parasympathetic nervous system and has been used to treat various diseases such as glaucoma.
In 1890, John Jacob Abel became the first pharmacology chair in the United States, at the University of Michigan. He later went to Johns Hopkins University in Baltimore. Abel was the first to isolate the hormone epinephrine from the adrenal gland, isolate histamine from the pituitary gland, and make pure crystalline insulin. Animals such as dogs, cats, pigeons, and frogs were used to test pharmacological substances. Humans were even used as test subjects. Sometimes they suffered through severe adverse effects from these substances, such as when the German pharmacist Friedrich Serturner and three of his friends had poisoning for several days from an alkaloid that Serturner had isolated from opium. This alkaloid was later named morphine, after the Ancient Greek god of sleep, Morpheus.
Oswald Schmiedeberg was a brilliant scientist. He studied the pharmacology of various compounds, including chloroform, and published an important text called the Outline of Pharmacology. There, he studied chloroform, which was used as an anesthetic, chloral hydrate, a sedative and hypnotic, and muscarine, a compound isolated from the mush to the field, Schmiedeberg is now known as 'the father of pharmacology'.
Non-Infectious Disease
Not caused by pathogens
Cannot be transmitted to other species
Malnutrition, Avitaminoses, Heavy Metals etc. are responsible
Risk factors:
Genetics
Life-style
Environmental factors
Genetic Risk Factors
Determined by genes
Familial Disease Tendency
Disease runs in species
Recessive gene disorders
Down syndrome
Born with extra chromosome
Sex-linked disorders
Linked to x chromosome (female)
Can be recessive in females
Color blindness, hemophilia, & muscular dystrophy
A brief presentation on fish vaccination and its application particularly in Bangladesh. The overall process is described in a nutshell here. The types, procedure of formation, regulation, licensing and use are among them.
The measures and methods adopted to secure a disease free environment in all phases of aquaculture practices (i.e. hatcheries, nurseries, grow-out farms) for improved profitability.
What is bacteria?
Bacteria shape
Types of bacteria
Difference between gram-positive and gram-negative bacteria
Common symptom
Causes
Bacterial disease in fish
Furunculosis
Columnaris
Dropsy
Vibriosis
Tuberculosis
Bacterial gill diseases
Fin rot / tail rot
There are many diseases of fish which can be troublesome to commercial producers as well as the recreational pond owner. Many disease outbreaks of captive fish stocks are associated with stressful conditions such as poor water quality, excessive crowding or inadequate nutrition.
Mycobacteriosis is a chronic or acute, systemic, granulomatous disease that occurs in aquarium and culture food fish, particularly those reared under intensive conditions. Mycobacteriosis results from infection by several species of Mycobacterium, aerobic, Gram-positive, pleomorphic rods which are members of the order Actinomycetales and family Mycobacteriaceae.
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2. PRESENTED BY
Naimul Hoque Sajib (ASH1602029M)
Debabrata paul (ASH1602030M)
Monoara Akter Lima (BKH1602033F)
Bipul Chandra Das (ASH1602034M)
Sudipta Singha Roy Antu (ASH1602036M)
Md. Atikuzzaman (ASH1602038M)
3. BACTERIA
• Unicellular or Single celled microscopic organisms.
• Cell membrane made up of cellulose and chitin.
• Vacuoles.
• Gram-positive and negative .
• Cell walls are thick and thin .
• Lack nuclei and other organized cell structure.
• Some bacteria are pigmented .
4. BACTERIAL DISEASE IN FISH
Fish are susceptible to a wide variety of bacterial pathogens.
Many of these bacteria capable of causing disease are considered by some to
be saprophytic in nature.
These bacteria only become pathogens when fishes are physiologically
unbalanced, nutritionally deficient, or there are other stressors, i.e., poor
water quality, overstocking, which allow opportunistic bacterial infections to
proceed.
5. LIST OF BACTERIAOF L DISEASE OCCURS IN FISHES
Furunculosis Disease
Columnaris Disease
Vibriosis Disease
Fin and Tail Rot Disease
Dropsy Disease
Cotton Mouth Disease
Tuberculosis Disease
Bacterial Gill Disease
Edwardsiella
Pseudomonasis
6. 1.FURUNCULOSIS:
Furunculosis of salmonids has been known for
many years and was the first bacterial disease to
have scientifically describe in 1894.
Its name is derived from the appearance of
ulcers in the skin.
Causative agent : Aeromonas salmonicida.
Susceptible species : Freshwater salmon, Rui etc.
7. Symptoms
According to Herman (1968) symptoms divided into four catagories :
i. Acute : Haemorrahagic, rapid mortality & bleeding in viscera.
ii. Sub-acute : Ulcerative form, deeply Haemorrahagic, fungal infect.
iii. Chronic : Red intestine and blood vessel enlarged, Haemorrahagic pyloric cocea.
iv. Latent : Sudden death, havely morality, no epitite, respiratory disrupted.
Treatment
Amoung antibiotics oxytetracycline are the best ; dosage : 2.5-3.5g/100pounds of fish per day.
Tetracyclin : 50-60g/kg for 5-7 days.
Cupper sulphate bath: 1: 2000 for 1minute for 4 days.
FURUNCULOSIS CONT...
8. Distributed worldwide.
A contagious and chronic disease.
It affects the external structures of the fish body.
Causal Agents :Flexibacter columnaris.
Susceptible Species :
• All freshwater fishes including goldfish, common carp,
grass carp, tilapia etc.
2.COLUMNARIS DISEASE :
9. Diagnosis :
Isolation and identification of the bacteria by biochemical, immunological and nuclear acid based
techniques.
Prophylaxis :
Prevent the movement of infected fish.
Avoiding overcrowding.
Feeding fishes with adequate supplementary feed.
Disinfect water and pond bottom.
Treatment : Salt bath 15% for 15 minutes.
COLUMNARIS DISEASE CONT …
10. This disease may occur in waters with low
oxygen.
These bacteria are small gram-negative bacilli,
characteristically curved.
Causal Agents :Vibrio bacteria.
Susceptible Species : vibriosis disease in salmon
and many other fishes.
3. VIBRIOSIS DISEASE
11. Diagnosis :
Identification based in histopathology.
Epithelial hyperplasia due to reaction in tissue.
Symptoms:
• Diseased fishes show large, bright coloured.
• bloody lesions in the skin and muscles, haemorrhages in eyes.
• gills may bleed with slight pressure, and inflammation of the intestinal tract.
Treatment : Sulfamethazine at a rate of 2 g / 100 pounds of fish / day gives good results. 3
– 4 g / 100 pounds of fish / day for 10 days of tetramycin also give satisfactory results.
VIBRIOSIS DISEASE CONT…
12. Well known bacterial disease.
It causes erosion and disintegration of fin and
tails.
It is common to all freshwater habitat.
• Causal Agents : Aeromonas sp, Pseudomonas spp
,Haemopophilus spp
• Susceptible Species : Carps, catfish, young fish
are more susceptible.
4. TAILAND FIN ROT DISEASE
13. Pathology :
Whitening area on the fin margin.
Lesions develop at the outer fin margin.
Frayed and disintegration of soft tissues between the fin rays.
Lost of the total fin.
Diagnosis :
Identification based in histopathology.
Epithelial hyperplasia due to reaction in tissue.
Treatment :
Tetracycline 50-60mg/kg of weight of fish with feed for 5-7 days.
TAILAND FIN ROT DISEASE CONT…
14. Dropsy is an abnormal accumulation of fluid
in the abdomen ,may affect the whole body or
in some organs & tissues .
Causal agents : Aeromonus hydrophila ,
Pseudomonas fluorescens
Susceptible species : Carps & others
Diagnosis :
Based on the typical clinical sign of abdominal
swelling & accumulation of fluid in body cavity
during necropsy.
5.DROPSY
15. Prophylaxis :
Disinfections of water & stocking materials.
Reducing stocking density .
Improving water quality & environment .
Treatment :
• Potassium permanganate 5ppm dip for 2 minutes .
• Sulphamerazine or sulphaguanadine or sulphadiazine or sulphanathazine or sulphaisexazol
10g/45kg fish /day as an empirical dose .
DROPSY CONT…
16. The filamentous bacteria, Flexi bacteria are the causative
agent of this disease.
The main symptom is appearance of fungus like tuft around
the mouth.
This can be treated with antibiotics like 10 ppm
chloramphenicol for 2-5 days and 0.3ppm furanace for long
term bath.
6. COTTON MOUTH DISEASE
17. Mycobacterium is a disease causing agent which is
difficult to diagnose without pathological
examinations.
The symptoms are ulcers on body, nodules in internal
organs, fin and tail rot, loss of appetite and loss of
weight of fish.
This can be cured with dip treatment in 1:2000 copper
sulphate for 1 minute for 3-4 days.
Antibiotics are not successful. The fishes should be
destroyed and potassium permanganate or lime used
in the pond.
7. TUBERCULOSIS DISEASE
18. Common in freshwater fish, both in cultivated and wild
fishes.
It is also known as Gill disease or environmental disease
as it is caused by an environmental irritant, which
damage the gill epithelium.
Causal Agents : Flexibacteria sp ,Pseudomonas sp,
Flavobacterium sp ,Aeromonas sp
Susceptible Species : All fishes, massive mortality occurs in
young stages of carps.
8. BACTERIAL GILL DISEASE
19. Symptoms
Show grasping.
Tendency to remain the surface and become lethargic.
Anorexic opercula become swollen.
Frayed appearance of affected gill tissues.
Excessive mucus secretions , opercula damage.
Diagnosis : Isolated and identification of agents under laboratory condition.
Prophylaxis : Tetracycline 50-60mg/kg of body weight of fish with feed for 5-7 days.
Treatment : Tetracycline 50-60mg/kg of body weight of fish with feed for 5-7 days.
BACTERIAL GILL DISEASE CON…
20. Known as edwardsiella septicaemia,serious syntenic
bacterial disease,cause mass mortality.
Causal agent: Edwardsiella tarda.
Susceptible species:
• Eels,catfish,tilapia and other species of warm water.
Sometimes of salmonids.
Larvae of catfish and carp in hatcheries.
9.EDWARDSIELLOSIS:
21. Symptoms
• Haemorrhage in fins and body.
• Rectal protrusion and swelling of the spleen.
• Lesions in visceral organs spread into the musculature and then to the dermis.
Prophylaxis: Avoidance of stress, disinfect water and pond bottom.
Treatment:
• Tetracycline chemotherapy 50mg/body wt. of fish/day for 5-7 days.
• Oxytetracycline 50mg/kg body weight of fish.
EDWARDSIELLOSIS CONT…
22. Known as pseudomonas septicaemia, may cause red spot
disease prevailing mostly in brackishwater.
Causal agents :Pseudomonas fluorescens ,Pseudomonas
anguilliseptica.
Susceptible Species :
Warm water fishes, wide variety of cultured and aquarium
fish mostly.
Diagnosis :n Isolation and identification of the bacteria.
10.PSEUDOMONASIS :
23. Pathology :
Haemorrhagic skin lesions or fin or tail rot.
Gross lesions include acites.
Exophthalmia.
Prophylaxis :
Avoidance of stress.
Maintain hygiene and sanitation.
Disposal of dead and infectious fishes.
Treatment : Sensitive recommended antibiotics at the requisite dose level.
PSEUDOMONASIS CONT…
24. Bacterial diseases are recognised as important hurdles in shrimp hatchery and farming.
Bacterial infections are usually occurred in shrimp farming in conjunction with other
diseases or processes or reflect an outcome of a breakdown in the ecological balance
within culture system.
Various bacterial genera mainly vibriosis and filamentous bacteria have been associated
with shrimp disease.
BACTERIAL SHRIMP DISEASE:
26. This infection is not recorded yet from Indian
waters, systemic rickettsial infections were
reported from cultured P. monodon from
Malaysia and Singapore.
In P. monodon, the rickettsia occurred within
large cytoplasmic vacuoles where it formed-
microcolonies of 19 to 33 f.lm in diameter
1. RICKETTSIAL INFECTIONS
27. In heavy infections, cells with rickettsial inclusions
were widespread in mesodermally and
ectodermally derived tissues, but absent in
endodermally derived tissues such as midgut,
hepatopancreas and caeca.
Experimental treatment using medicated feeds
containing 1.5 to 2. 0 kg of oxytetracycline per 1000
kg offered was found to be successful in reducing
monalities
RICKETTSIAL INFECTIONS CONT…
28. Vibriosis is a common bacterial diseases of
shrimps.
A gram negative, oxidase positive, motile
rod type bacteria
Causal agents: V. Harvesting ,V. Vulnificus, V.
Parahaemolyticus, V. Alginolytcus
Susceptible species:
• All stages of all cultured shrimp are
susceptible in stressful conditions.
2.VIBRIOSIS:
29. Pathology:
The common pathological sign associated with vibriosis is high mortality, moribund shrimp
appearance in hypoxic and often come into pond surface.
Redding of shrimp,shell and appendages necrosis with blackening.
Presence of luminescence in affected shrimp in pond.
Treatment:
• Disinfection of intake water i.e. formalin 100-200 ppm. Anti-microbial preparation application
through feeds (Oxolinic acid 0.6 ppm and Sarafloxacin 5 mg/kg).
VIBRIOSIS CONT …
30. The above-referred names are synonyms
of bacterial disease caused by a group of
bacteria.
The disease is recorded from the
freshwater prawns as well as from
Penaeus sp. cultured in India.
Causal agents: Vibrio sp, Pseudomonas sp
and Beneckea sp .
3.BROWN SPOT SHELL DISEASE
31. Control measure: Providing better water quality, removal of infected and dead prawns,
reducing the stock and adequate nutrition help to control the disease.
Treatment:
Feeding terramycin incorporated feed at 0.45 mg per kg of feed for two weeks, bath
treatment using 0.05 to 1. 0 mg of malachite green per litre of water are suggested.
BROWN SPOT SHELL DISEASE CON…
32. i. Perform regular water changes.
ii. Keep the tank clean.
iii. Clean the filter regularly.
iv. Avoid overcrowding the tank.
v. Use flake foods within one month of opening.
vi. Use disinfect nets and other equipments.
• If the tank is well maintained, and the fish fed a healthy diet, outbreaks of any bacterial disease
are unlikely..
PREVENTION: