This document summarizes screening methods for central and peripheral analgesics. For central analgesics, it describes in vivo methods like Haffner's tail clip, hot plate, tail immersion, and formalin tests that assess response to painful stimuli in mice and rats. For peripheral analgesics, it discusses writhing tests using acetic acid or phenylquinone in mice, Randall-Selitto testing in inflamed rat paws, and duodenum distension in rats to measure visceral pain responses. The document provides classifications and examples of different classes of central and peripheral analgesic agents and their mechanisms of action.
Screening methods of immunomodulators by shivam diwakerShivam Diwaker
Immune Modulators are the substances or drugs or chemical compounds that are used for the modification in the Immune system such as stimulate and suppress.
In this slide contains diabetics, classification, symptoms, complication, invivo and invitro screening models of anti diabetics.
Presented by: GEETHANJALI ADAPALA (Department of pharmacology).
RIPER, anantapur
Screening methods of immunomodulators by shivam diwakerShivam Diwaker
Immune Modulators are the substances or drugs or chemical compounds that are used for the modification in the Immune system such as stimulate and suppress.
In this slide contains diabetics, classification, symptoms, complication, invivo and invitro screening models of anti diabetics.
Presented by: GEETHANJALI ADAPALA (Department of pharmacology).
RIPER, anantapur
screening models for anxiolytics with detailed procedure and evaluation,
detailed classification about methods, pathophysiology of anxiety, components of anxiety, validity of anxiety,
difference bet pathological and physiological anxiety, different theories of anxiety, criteria of animal model, pharmacological manipulations, conditioned behavior, unconditioned behavior
Expt. 6 Study of effect of drugs on gastrointestinal motilityVISHALJADHAV100
Objective
Principle
Requirements
Preparation of Tyrode solution
Procedure
Kymograph recording of contractions
Observation table
Result and Interpretation
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists Saeid Safari
Preoperative Management of Patients on GLP-1 Receptor Agonists like Ozempic and Semiglutide
ASA GUIDELINE
NYSORA Guideline
2 Case Reports of Gastric Ultrasound
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
2. Contents
Definitions
Central analgesics : agents, mechanism, in vivo
methods
Peripheral analgesics : agents, mechanism, in vivo
methods
3. Definitions
Pain- an unpleasant sensory or emotional experience
associated with actual or potential tissue damage, or described
in terms of such damage (WHO).
Features of inflammation
Pain (dolor)
rubor (redness)
tumor (swelling)
calor (heat)
functio laesa (loss of function).
4. Analgesics – the drugs which possess significant pain-
relieving properties by acting in the central nervous
system or on peripheral pain receptors without
significantly affecting consciousness.
Two groups :
I. Narcotic/opioid /morphine like analgesics(central)
II. Non-narcotic/ non steroidal anti-inflammatory-
analgesic-antipyretic agents(peripheral)
8. Haffner’s tail clip method, 1929
The test compounds are administered subcutaneously to
fed mice or orally to fasted animals.
Then an artery clip is applied to the root of the tail
(approximately 1 cm from the body) of mice.
The reaction time is noted.
Response : biting the clip or the tail near the location of the
clip.
9. Hot plate method
Woolfe and Mac Donald (1944)
Mice are put on hot plate(temp 55° to 56 °C) and then
latency is noted following administration of test drug.
Response – jumping or paw licking
10. Tail immersion method
Restraining the young female wistar rat, lower 5 cm
portion of the tail is immersed in a cup of freshly filled
water of exactly 55°C.
The reaction time is determined before and periodically
after either oral or subcutaneous administration of the
test substance.
The cut off time of the immersion is 15 seconds.
Response – withdrawal of tail or attempt to escape.
11. Grid shock test
Blake et al(1963)
Central and peripheral analgesics
Stimulus is given in the form of square wave pulses to male
mice. The output of the stimulator is connected to the wires of
grid with fixed resistance , which is kept parallel to
oscilloscope.
Pain thresholds are determined in each individual mouse
twice before and after administration of the test drug.
Response – on oscilloscope, a startling reaction, increase
locomotion or attempt to jump.
12. Electrical stimulation of the tail
Burn et al, 1950
Central and peripheral analgesics
First test compound is given and then rectangular wave
pulses from a constant voltage stimulator are applied to
the alligator clips attached to the tail of the mice.
Response – spinal reflexes, complete vocalization or
behavior changes(escape, aggression). The normal
response time range of the stimuli is 3–4 sec.
13. Tooth pulp stimulation
Kohl and Reffert (1938)
Opioid agonists, pyrazolone derivatives
After anaesthetizing the rabbit with thiopental, current is
applied through the electrodes passed in the dental drill
holes exposed to pulp chambers.
Pain thresholds are determined in each individual rabbit
twice before and after administration of the test drug.
Response - licking, biting, chewing or head flick.
14. Formalin test in rats
Dubuisson and Dennis,1977
Male wistar rats are administered 0.05 ml of 10%
formalin in dorsum of the front paw along with test drug
subcutaneously or orally.
Response - elevation or favoring of the paw or
excessive licking and biting of the paw.
19. Writhing tests
Mice are administered the test drug before intraperitoneal
injection of irritating agent like phenylquinone or acetic
acid.
Pain sensation in acetic acid induced writhing paradigm is
elicited by producing localized inflammatory response due
to release of free arachidonic acid from tissue phospholipids
via cyclo-oxygenase (COX), and producing prostaglandin
specifically PGE2 and PGF2α, the level of lipoxygenase
products may also increases in peritoneal fluids.
20. Response – writhe (stretching of the abdomen with
simultaneous stretching of at least one hind limb)
Aconitine can also be used instead of above drugs.
Rats – 4% sodium chloride.
21. Pain in inflamed tissue
(RANDALL-SELITTO TEST)
Principle - inflammation increases the sensitivity to pain and
that this sensitivity is susceptible to modification by
analgesics.
Male wistar rats are given the test drug orally,
subcutaneously or intraperitoneally.
Inflammation is induced by subcutaneous injection of
Brewer’s yeast into the plantar surface of the left hind paw of
the rat .Then pressure is applied through a tip to the plantar
surface of the rat’s foot at a constant rate by a special
apparatus to the point at which the animal struggles, squeals
or attempts to bite.
22. Mechanical visceral pain model in the rat
Coburn et al 1989,deLeo et al 1989.
First test drug is administered into male Sprague Dawley
rats and anaesthetized with nitrous oxide and halothane.
One piece balloon catheter is passed through
gastrostomy tube into first part of duodenum.
Inflation of balloon distension of duodenum
writhing.
23. Behavioural responses score
0 : Normal behaviour defined as exploration, escape attempts
and resting
1 : Slightly modified behaviour defined as cessation of
exploration, focusing, wet-dog shake, excessive facial
grooming, teeth chattering and deep breathing
2 : Mildly to moderately modified behaviour defined as
retching-like activity, hunching, abdominal grooming or
nipping and immobility of the hind limbs (disappears with
removal of the stimulus).
24. 3 : Severely modified behaviour defined as stretching of
the hind limbs, arching and dorsoflection of the hind
paws.
4 : Intensive visceromotor activity defined as repetitive
stretching of the body, extension of the hind limbs, and
pelvis, frequent rotating sideward , i.e., writhing.
25. References
H. Gerhard Vogel et al. Drug discovery and evaluation :
Pharmacological assays, third edition.
K D Tripathi. Essentials of medical pharmacology,
seventh edition.
H L Sharma, K K Sharma. Principles of pharmacology,
second edition.
Harsh Mohan. Textbook of pathology, sixth edition.