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Development and evaluation of a maize
variety with resistance against
Pink stem borer (Sesamia nanogrioides)
And European corn borer (Ostrinia nubilalis)



              Mehmet Ali TURKAY 1
1East Mediterranen Agricultural Research İnstitute-

                 Adana/TURKEY


         I would like to present the results of the study of
Seventy percent
One of them is (%70) of planting area and ninety percent (%90) of the total
Pesticides insects are found in the second crop season and one in the first
While both are applied three times in Mediterranean and the Aegean region, but
production area (Sesamia nanogrioides)
Pink stem borer of maize of our country has been affected from these two
crop season for these insects.
European corn borer can just be found in Black Sea region of Turkey
insects.
and another one is European corn borer (Ostrinia nubilalis)
There are two major insects that negatively affect maize production in Turkey.
While European corn five generations in the Çukurova region of to 30-40 cm,
Pink stem borer gives borer demages plant after the plant grow Turkey, while
Pink stem borer can has three every period
European corn borerdemage in generations of growing.
TÜRKAY                                       CHECK
An experiment with and without chemaical applications in second crop
The results of an experiment that carried out without chemical application in
Tunnel length of plant of the two
For talk on the results method different
I willevaluationperthe and evaluation of the experiments
Secondly, the yield resistance was used resistance
Firstly, crop 2010
I will explain conditions in 2009- 2010
conditions in
second
Table1. The Yield and Tunnel Length Values of The Pesticide-Free
Experiment That Carried Out İn Second Crop Conditions İn 2009-2010 (t/ha)
Variety    Grain Gr  Tünnel Gr           Variety   Grain Gr  Tünnel        Gr
           Yield     Length/                       Yield     Length/
           (t/ha)   Plant (cm)                     (t/ha)   Plant (cm)
TÜRKAY      10.5   a      1.0       h    25Ax10      6.0   g      3.2       h
22x9B       10.1   a      0.8       h    DK 6022     5.0   h      43.2     de
22x5A       10.0   a      1.0       h    P32W86      4.8   hı     53.4     ab
24Ax10       9.0   b      0.4       h    SİDE        4.7   hı     45.6     cd
9Bx24A       8.3   bc     0.2       h    DK 5783     4.5   hj     47.4     cd
5Ax25A       8.0   cd     0.8       h                             50.4     bc
                                         P 3394      4.1   hj
KARAÇAY      7.5   ce     35.0      f
                                             DK 626    4.0 ıj CHECK
                                                                48.7        bc
18Ax5A CHECK df
              7.3           16.2 TÜRKAY g                       50.5        bc
                                             HELEN     3.6 j
GÖZDEM        7.3 df        40.3        e
                                             7C2x9A    2.7 k    56.9         a
9Bx25A yield of theper plant werein hchecks 3.6 andbetween 43.2 cm and That
                             1.2
Two hundred6.9Tunnel Length infected of The Pesticide-Free Experiment 53.4
The Yield andthat eg checks of the the region, naturaltolerantand yield of five
While the
Nine checks lengthwidelyplant Values from varied 5.0 ton/ha, the twenty the
       tunnel larvas per grown between the three fauna genotype, ten
The yield of the tolerant hybrids varied from
Any pesticide was not used for this study 4.7 to 7.5 ton/ha
cm, the Out İn inTÜRKAY variety
Carried length Second Crop Conditions İn 2009-used as plant material
TÜRKAY plant
experimental 6.4 was and TÜRKAY variety P<0,01
larvas pervarietyinfected artifically was onlywere 2010CV 12.0
22x10          hybrids 10.5 ton/ha
                     fg      4.0        h      1 cm.             17.0
Table 2. Yield losses of the experiment which carried out with
pesticide and pesticide free in second crop season in 2010 (t/ha)
             Pesticide-    Pesticide                  Pesticide-    Pesticide
                 Free                                     Free
Variety     Grain Grp Grain Grp           Variety    Grain    Grp Grain Grp
            Yield     Yield                          Yield        Yield
            (t/ha)    (t/ha)                         (t/ha)       (t/ha)
TÜRKAY       11.0    ab    11.4     a     DK6022       3.7     ı     7.3     h
22X9B        10.2    ad    10.7    ac     P3394        3.6     ı     7.4    fh
22X5A        10.2    ad    10.9    ac
                                          P32W86       3.5     ı     8.6    dh
24AX10        9.1    cg     9.4    bf
                                          HELEN        3.3     ı     7.0     h
5AX25A        8.2    eh     8.5    dh
9BX24A        8.0    eh     8.5    dh     DK 5783      3.3     ı     7.0     h
KARAÇY        7.3    gh     9.6    ae     DK626   3.0          ı     8.0    eh
18AX5A       CHECK gh
              7.3           7.7    fh        TÜRKAY
The yield of the the TÜRKAY9.3 which carried out with pesticide between 7 and
GÖZDEM of checks in pesticide bf
Yield losses 7.0 experiment
In this experiment     h     varietyapplicated conditions varied free condition,
                                      had 11 ton/ha in pesticide and pesticide
Theton/haof the checks in pesticide-free varied between 3 and 3.7 ton/ha 14.0
     yield
8.6 in secondpesticde applicated condition P<0,01                    CV
11.4
free ton/ha in 3.8 season in 2010 eh
SİDE            crop ı      8.0
Table 3. Yield losses as % in the pesticide-free and pesticide
applicated experiment




        70
        60
        50
        40
        30
        20
        10
         0




    SUSCEPTİBLE LİNE                             RESİSTANT LİNE
Yield losses as percent (%) in the pesticide-free and pesticide applicated
While yield losses in the checks varied between % 50 and % 65, just % 3 yield
conditions
loss was recorded for TÜRKAY variety
Table 4. Pesticide applicated and Pesticide-free trial values of
tunnel length per plant (cm)
           Pesticide-     Pesticide                Pesticide-     Pesticide
               Free                                    Free
Variety    Tünnel    Gr       TÜRKAY Variety
                          Tünnel Gr                Tünnel Gr Tünnel Gr
           Length         Length/                  Lengt/    Length/
            /Plant         Plant                   Plant      Plant
DK 5783      61.2    a     6.1    hj   18AX5A        13.3    f     2.6     ıl
P32W86       58.9    ab    7.5    gı   5AX25A        1.4    fg     0.1      l
DK6022       57.3    ab    5.1    hk   22X5A         0.5     jl    0.0      l
HELEN        57.2    ab    2.4    jl   9BX24A        0.4    kl     0.0      l
P3394        54.4    bc    13.1   fg    CHECK
                                       24AX10        0.3    kl     0.6     kl
DK626        50.5    cd    8.3    gh   22X9B         0.3    kl     0.0      l

SİDE         49.4    d     9.6    fh   TÜRKAY        0.2    kl     0.0      l

GÖZDM         47.5    d     3.2      ıl
Because it intensive insect these values must % 60 of the plant pesticide
However of is supposed that population, maximal be zero (0) for the protected
                                                                          17.0
apllicated areas pesticide checks varied P<0,01
The demage values and Pesticide-free from of to 13.1
KARAÇY        35.6    e     9.1     fh
                                                                     CV
Pesticide applicated of theapplication values2.4 tunnel length per plant
with three times
As a Result




It can be variety has high yield is resistant to both the pesticide corn borer
TÜRKAY seen, TÜRKAY varietyin both pesticide-free orEuropean applicated
                    CHECK
As aPink stem borer.                                                TÜRKAY
fieldsResult
and
TÜRKAY variety
The ear of the TÜRKAY variety
TÜRKAY
       CHECK

Green TÜRKAY variety and checks that affected from the insects
CHECK                      TÜRKAY



Standing TÜRKAY variety but broken and a lodging check
TÜRKAY
         CHECKS




While TÜRKAY is green, two checks that in the border of the experiment are dead early
CHECK
                                                            TÜRKAY

Both checks are broken and lying, while TÜRKAY variety is standing
TÜRKAY
            CHECK

A check that grown beside the TÜRKAY in another replication is dead but Turkay variety is green.
TÜRKAY                                  CHECK


Check broken and lying but whole plants of TÜRKAY variety is standing
RESİSTANT      SUSCEPTİBLE
                                 RESİSTANT
                                                 SUSCEPTİBLE




    We can see the demage of the European corn borer and Pink stem borer
TÜRKAY




There is no demage on TÜRKAY variety’s ear but demage on check’s ear can bee seen
TÜRKAY

The vascular bundle of the TÜRKAY variety
The vascular bundle of the CHECKS
TÜRKAY




                                                           CHECK




The vascular bundle of the TÜRKAY variety is healthy but vascular bundle of the checks like an
empty pipe
SUSCEPTİBLE LİNE                    RESİSTANT LİNE

  Resistant line is the parent of the TÜRKAY variety
Movingİmge




SUSCEPTIBLE LINE
                                         SUSCEPTIBLE LINE

                    RESISTANT LINE



Resistant lines is standing on the harvest time but it is not
possible to see susceptible lines because they were all dead.

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S2.5 Development and evaluation of a maize variety with resistance against

  • 1. Development and evaluation of a maize variety with resistance against Pink stem borer (Sesamia nanogrioides) And European corn borer (Ostrinia nubilalis) Mehmet Ali TURKAY 1 1East Mediterranen Agricultural Research İnstitute- Adana/TURKEY I would like to present the results of the study of
  • 2. Seventy percent One of them is (%70) of planting area and ninety percent (%90) of the total Pesticides insects are found in the second crop season and one in the first While both are applied three times in Mediterranean and the Aegean region, but production area (Sesamia nanogrioides) Pink stem borer of maize of our country has been affected from these two crop season for these insects. European corn borer can just be found in Black Sea region of Turkey insects. and another one is European corn borer (Ostrinia nubilalis) There are two major insects that negatively affect maize production in Turkey.
  • 3. While European corn five generations in the Çukurova region of to 30-40 cm, Pink stem borer gives borer demages plant after the plant grow Turkey, while Pink stem borer can has three every period European corn borerdemage in generations of growing.
  • 4. TÜRKAY CHECK An experiment with and without chemaical applications in second crop The results of an experiment that carried out without chemical application in Tunnel length of plant of the two For talk on the results method different I willevaluationperthe and evaluation of the experiments Secondly, the yield resistance was used resistance Firstly, crop 2010 I will explain conditions in 2009- 2010 conditions in second
  • 5. Table1. The Yield and Tunnel Length Values of The Pesticide-Free Experiment That Carried Out İn Second Crop Conditions İn 2009-2010 (t/ha) Variety Grain Gr Tünnel Gr Variety Grain Gr Tünnel Gr Yield Length/ Yield Length/ (t/ha) Plant (cm) (t/ha) Plant (cm) TÜRKAY 10.5 a 1.0 h 25Ax10 6.0 g 3.2 h 22x9B 10.1 a 0.8 h DK 6022 5.0 h 43.2 de 22x5A 10.0 a 1.0 h P32W86 4.8 hı 53.4 ab 24Ax10 9.0 b 0.4 h SİDE 4.7 hı 45.6 cd 9Bx24A 8.3 bc 0.2 h DK 5783 4.5 hj 47.4 cd 5Ax25A 8.0 cd 0.8 h 50.4 bc P 3394 4.1 hj KARAÇAY 7.5 ce 35.0 f DK 626 4.0 ıj CHECK 48.7 bc 18Ax5A CHECK df 7.3 16.2 TÜRKAY g 50.5 bc HELEN 3.6 j GÖZDEM 7.3 df 40.3 e 7C2x9A 2.7 k 56.9 a 9Bx25A yield of theper plant werein hchecks 3.6 andbetween 43.2 cm and That 1.2 Two hundred6.9Tunnel Length infected of The Pesticide-Free Experiment 53.4 The Yield andthat eg checks of the the region, naturaltolerantand yield of five While the Nine checks lengthwidelyplant Values from varied 5.0 ton/ha, the twenty the tunnel larvas per grown between the three fauna genotype, ten The yield of the tolerant hybrids varied from Any pesticide was not used for this study 4.7 to 7.5 ton/ha cm, the Out İn inTÜRKAY variety Carried length Second Crop Conditions İn 2009-used as plant material TÜRKAY plant experimental 6.4 was and TÜRKAY variety P<0,01 larvas pervarietyinfected artifically was onlywere 2010CV 12.0 22x10 hybrids 10.5 ton/ha fg 4.0 h 1 cm. 17.0
  • 6. Table 2. Yield losses of the experiment which carried out with pesticide and pesticide free in second crop season in 2010 (t/ha) Pesticide- Pesticide Pesticide- Pesticide Free Free Variety Grain Grp Grain Grp Variety Grain Grp Grain Grp Yield Yield Yield Yield (t/ha) (t/ha) (t/ha) (t/ha) TÜRKAY 11.0 ab 11.4 a DK6022 3.7 ı 7.3 h 22X9B 10.2 ad 10.7 ac P3394 3.6 ı 7.4 fh 22X5A 10.2 ad 10.9 ac P32W86 3.5 ı 8.6 dh 24AX10 9.1 cg 9.4 bf HELEN 3.3 ı 7.0 h 5AX25A 8.2 eh 8.5 dh 9BX24A 8.0 eh 8.5 dh DK 5783 3.3 ı 7.0 h KARAÇY 7.3 gh 9.6 ae DK626 3.0 ı 8.0 eh 18AX5A CHECK gh 7.3 7.7 fh TÜRKAY The yield of the the TÜRKAY9.3 which carried out with pesticide between 7 and GÖZDEM of checks in pesticide bf Yield losses 7.0 experiment In this experiment h varietyapplicated conditions varied free condition, had 11 ton/ha in pesticide and pesticide Theton/haof the checks in pesticide-free varied between 3 and 3.7 ton/ha 14.0 yield 8.6 in secondpesticde applicated condition P<0,01 CV 11.4 free ton/ha in 3.8 season in 2010 eh SİDE crop ı 8.0
  • 7. Table 3. Yield losses as % in the pesticide-free and pesticide applicated experiment 70 60 50 40 30 20 10 0 SUSCEPTİBLE LİNE RESİSTANT LİNE Yield losses as percent (%) in the pesticide-free and pesticide applicated While yield losses in the checks varied between % 50 and % 65, just % 3 yield conditions loss was recorded for TÜRKAY variety
  • 8. Table 4. Pesticide applicated and Pesticide-free trial values of tunnel length per plant (cm) Pesticide- Pesticide Pesticide- Pesticide Free Free Variety Tünnel Gr TÜRKAY Variety Tünnel Gr Tünnel Gr Tünnel Gr Length Length/ Lengt/ Length/ /Plant Plant Plant Plant DK 5783 61.2 a 6.1 hj 18AX5A 13.3 f 2.6 ıl P32W86 58.9 ab 7.5 gı 5AX25A 1.4 fg 0.1 l DK6022 57.3 ab 5.1 hk 22X5A 0.5 jl 0.0 l HELEN 57.2 ab 2.4 jl 9BX24A 0.4 kl 0.0 l P3394 54.4 bc 13.1 fg CHECK 24AX10 0.3 kl 0.6 kl DK626 50.5 cd 8.3 gh 22X9B 0.3 kl 0.0 l SİDE 49.4 d 9.6 fh TÜRKAY 0.2 kl 0.0 l GÖZDM 47.5 d 3.2 ıl Because it intensive insect these values must % 60 of the plant pesticide However of is supposed that population, maximal be zero (0) for the protected 17.0 apllicated areas pesticide checks varied P<0,01 The demage values and Pesticide-free from of to 13.1 KARAÇY 35.6 e 9.1 fh CV Pesticide applicated of theapplication values2.4 tunnel length per plant with three times
  • 9. As a Result It can be variety has high yield is resistant to both the pesticide corn borer TÜRKAY seen, TÜRKAY varietyin both pesticide-free orEuropean applicated CHECK As aPink stem borer. TÜRKAY fieldsResult and
  • 11. The ear of the TÜRKAY variety
  • 12. TÜRKAY CHECK Green TÜRKAY variety and checks that affected from the insects
  • 13. CHECK TÜRKAY Standing TÜRKAY variety but broken and a lodging check
  • 14. TÜRKAY CHECKS While TÜRKAY is green, two checks that in the border of the experiment are dead early
  • 15. CHECK TÜRKAY Both checks are broken and lying, while TÜRKAY variety is standing
  • 16. TÜRKAY CHECK A check that grown beside the TÜRKAY in another replication is dead but Turkay variety is green.
  • 17. TÜRKAY CHECK Check broken and lying but whole plants of TÜRKAY variety is standing
  • 18. RESİSTANT SUSCEPTİBLE RESİSTANT SUSCEPTİBLE We can see the demage of the European corn borer and Pink stem borer
  • 19. TÜRKAY There is no demage on TÜRKAY variety’s ear but demage on check’s ear can bee seen
  • 20. TÜRKAY The vascular bundle of the TÜRKAY variety
  • 21. The vascular bundle of the CHECKS
  • 22. TÜRKAY CHECK The vascular bundle of the TÜRKAY variety is healthy but vascular bundle of the checks like an empty pipe
  • 23. SUSCEPTİBLE LİNE RESİSTANT LİNE Resistant line is the parent of the TÜRKAY variety
  • 24. Movingİmge SUSCEPTIBLE LINE SUSCEPTIBLE LINE RESISTANT LINE Resistant lines is standing on the harvest time but it is not possible to see susceptible lines because they were all dead.