This document proposes a study on factors of resistance in maize plants against the spotted stem borer, Chilo partellus. The study will assess molecular diversity and biochemical/nutrient levels in maize cultivars to establish correlations with borer infestation levels. It will also examine physical resistance factors like plant architecture, stem diameter, and leaf trichome density. The research will involve growing 26 maize genotypes and quantifying borer damage, molecular profiles, nutrient levels, and physical traits to identify sources of resistance.
Pulses are a very important source of protein in Indian diets as majority of population is vegetarian. however, the production of pulses is not keeping pace with the growing population in the country. lentil is one of the important Rabi pulses. it is one of the oldest pulse crops and the most nutritious of the pulses. it is also used as a cover crop to check the soil erosion in problem areas. lentil contributes about 6% in total pulses area as well as production of India. It is mostly eaten as "DAL". the pulse is first converted into split pulse or 'dal' by the removal of skin and the separation of the fleshy cotyledons. It is cooked easily and hence preferred. It is good for patients too. Lentil contains about 11% water, 25% protein and 60% carbohydrates. It is also rich in calcium, iron and niacin
28. Breeding for resistance to abiotic stresses – drought resistance – mechanisms of drought resistance (drought escape, avoidance, tolerance, and resistance) – features associated with drought resistance – sources of drought resistance – breeding methods for drought resistance – limitations – achievements; breeding for resistance to water logging – effects of water logging mechanism of tolerance – ideotype for flooded areas – breeding methods.
Recent advances in the methods of fertilizer scheduling in temperate fruitsJahangeer Ahmad
Fertilizer scheduling is the process of determining when to give fertilizer and how much of fertilizer to be applied.
Scientific fertilizer scheduling is a technique providing knowledge on correct time and optimum quantity of fertilizer application at each stage to optimize crop yields with maximum fertilizer use efficiency and at the same time ensuring minimum damage to soil properties.
Twice repeated experiment was carried out under laboratory conditions to investigate the allelopathic effects of aqueous extracts of Parthenium hysterophorus L. shoot (stem + branch) and leaf, at 0, 5, 10 and 15 g L-1 (w/v) concentrations on maize (Zea mays L.) seed germination, seedling growth (shoot and root length) and biomass production. The treatments were laid out in completely randomized design with the factorial arrangement in four replications. Result indicated that the highest germination percentage (98.75%) was recorded from control whereas the lowest (43.75 %) was from stem extract at15 g L-1 concentration level. Similar trend was also observed by leaf extract. Root and shoot length of maize crop was reduced by 91.4 % and 70.8% by 85.6 and 35.8% leaf extracts and stem extracts respectively hence the roots were more sensitive to allelopathic effect than shoot. Extract of both leaf and stem at 15 g L-1 strongly reduced fresh and dry biomass of the maize seedling. The highest (100,100 %) tolerance index was recorded from control whereas, the lowest (8.62, 14.74%) was recorded from leaf and stem extract at 15 g L-1 concentration level respectively. Leaf aqueous extract showed more phytotoxic effect (91.37 %) than stem extracts (85.25) at 15 g L-1 whereas was the minimum recorded in control (0.0%). 15 g L-1 minimum value of vigor index (200, 539.2) and higher inhibition potentials (62.5 and 55.78%) were recorded from leaf and stem respectively. therefore allelopathic effect by the parthenium extracts is concentration-dependent manner. Leaf extract had shown highest inhibition potential followed by the stem.
Pulses are a very important source of protein in Indian diets as majority of population is vegetarian. however, the production of pulses is not keeping pace with the growing population in the country. lentil is one of the important Rabi pulses. it is one of the oldest pulse crops and the most nutritious of the pulses. it is also used as a cover crop to check the soil erosion in problem areas. lentil contributes about 6% in total pulses area as well as production of India. It is mostly eaten as "DAL". the pulse is first converted into split pulse or 'dal' by the removal of skin and the separation of the fleshy cotyledons. It is cooked easily and hence preferred. It is good for patients too. Lentil contains about 11% water, 25% protein and 60% carbohydrates. It is also rich in calcium, iron and niacin
28. Breeding for resistance to abiotic stresses – drought resistance – mechanisms of drought resistance (drought escape, avoidance, tolerance, and resistance) – features associated with drought resistance – sources of drought resistance – breeding methods for drought resistance – limitations – achievements; breeding for resistance to water logging – effects of water logging mechanism of tolerance – ideotype for flooded areas – breeding methods.
Recent advances in the methods of fertilizer scheduling in temperate fruitsJahangeer Ahmad
Fertilizer scheduling is the process of determining when to give fertilizer and how much of fertilizer to be applied.
Scientific fertilizer scheduling is a technique providing knowledge on correct time and optimum quantity of fertilizer application at each stage to optimize crop yields with maximum fertilizer use efficiency and at the same time ensuring minimum damage to soil properties.
Twice repeated experiment was carried out under laboratory conditions to investigate the allelopathic effects of aqueous extracts of Parthenium hysterophorus L. shoot (stem + branch) and leaf, at 0, 5, 10 and 15 g L-1 (w/v) concentrations on maize (Zea mays L.) seed germination, seedling growth (shoot and root length) and biomass production. The treatments were laid out in completely randomized design with the factorial arrangement in four replications. Result indicated that the highest germination percentage (98.75%) was recorded from control whereas the lowest (43.75 %) was from stem extract at15 g L-1 concentration level. Similar trend was also observed by leaf extract. Root and shoot length of maize crop was reduced by 91.4 % and 70.8% by 85.6 and 35.8% leaf extracts and stem extracts respectively hence the roots were more sensitive to allelopathic effect than shoot. Extract of both leaf and stem at 15 g L-1 strongly reduced fresh and dry biomass of the maize seedling. The highest (100,100 %) tolerance index was recorded from control whereas, the lowest (8.62, 14.74%) was recorded from leaf and stem extract at 15 g L-1 concentration level respectively. Leaf aqueous extract showed more phytotoxic effect (91.37 %) than stem extracts (85.25) at 15 g L-1 whereas was the minimum recorded in control (0.0%). 15 g L-1 minimum value of vigor index (200, 539.2) and higher inhibition potentials (62.5 and 55.78%) were recorded from leaf and stem respectively. therefore allelopathic effect by the parthenium extracts is concentration-dependent manner. Leaf extract had shown highest inhibition potential followed by the stem.
The Antibiotic and Antixenotic Resistance of Some Peanut (Arachis hypogea L.)...IJRES Journal
Resistant varieties is one of the important components in integrated pest management. Studies of the Antixenotic and antibiotic resistance of peanut (Arachis hypogea L.) varieties aims to obtain varieties resistant and suitable to be cultivated in the province of North Sulawesi. Research using factorial experiment, treatment peanut varieties: Local varieties (V1), Giraffe (V2), Bison (V3), Bima (V4), Elephant (V5) and Rabbit (V6). Organic fertilizer treatments: Without fertilization (P0) and Super Petroganik as much as 20 tonnes/ha (P1) with three replications. Application of organic fertilizer made one month before planting seeds. Antixenosis and antibiosis resistance research results at 30 days after planting (DAP), the highest stomata diameter contained in P1V1 treatment is 12.62 μm, the longest trichomes on P0V3 is 88.07 μm, the highest stem cotrtex on P1V2 is 88.30 μm, and the highest contained on P1V6 is 9.96 mg/g. The result of the 60 DAP is obtained that the highest yields diameter stomata, trichomes length and content of flavonoids in P1V6 each is 13.85 μm, 136.33 μm and 14:57 mg/g, being the highest cortex diameter on P1V5 is 158.80 μm. Based on the results of analysis of variance, flavonoid content peanut varieties 30 DAP and 60 DAP significantly different (P < 0.05), while the diameter of stomata, trichomes numbers and length number, and corticial stem not significant (P > 0.05). Resistance mechanisms developed peanut plants are antibiosis.
Identification and evaluation of antifungal compounds from botanicals for the...researchagriculture
Red rot is a devastating disease in sugarcane caused by fungus, Colletotrichum falcatum. In this study, eighteen different botanicals were screened for identifying effective antifungal compound against C. falcatum. Among the plants screened, 15 per cent aqueous leaf extract of Psoralea corylifolia alone inhibited 100 per cent growth of both mycelium as well as spore germination under in vitro conditions. The extract did not exhibit any inhibitory effect to the beneficial microbes viz., Pseudomonas fluorescens, Bacillus megaterium and Gluconacetobacter diazotrophicus which are normally used in sugarcane. The effective plant extracts exhibiting 100 per cent antifungal activity was subjected to TLC, HPLC and GC-MS analysis to identify the bioactive antifungal compound. It revealed the presence of 7H-furo [3,2-G] (1) benzopyran-7-one as main bioactive compound which is thought to be the intermediate of antifungal compound, 8 – methoxypsoralen formed during biosynthesis.
Article Citation:
Rajkumar D and Murugesan R.
Identification and Evaluation of Antifungal Compounds from Botanicals for the Control of Sugarcane Red Rot Pathogen, Colletotrichum falcatum.
Journal of Research in Agriculture (2013) 2(1): 164-172.
Full Text:
http://www.jagri.info/documents/AG0044.pdf
Identification and Evaluation of Antifungal Compounds from Botanicals for th...researchagriculture
Red rot is a devastating disease in sugarcane caused by fungus,
Colletotrichum
falcatum
. In this study, eighteen different botanicals were screened for
identifying effective antifungal compound against
C.
falcatum.
Among the plants
screened, 15 per cent aqueous leaf extract of
Psoralea corylifolia
alone inhibited 100
per cent growth of both mycelium as well as spore germination under
in vitro
conditions. The extract did not exhibit any inhibitory effect to the beneficial microbes
viz.
,
Pseudomonas fluorescens
,
Bacillus megaterium
and
Gluconacetobacter
diazotrophicus
which are normally used in sugarcane. The effective plant extracts
exhibiting 100 per cent antifungal activity was subjected to TLC, HPLC and GC
-
MS
analysis to identify the bioactive antifungal compound. It revealed the
presence of
7H
-
furo [3,2
-
G] (1) benzopyran
-
7
-
one as main bioactive compound which is thought to be
the intermediate of antifungal compound, 8
–
methoxypsoralen formed during
biosynthesis.
Evaluation of four plants compost teas against fungi responsible for Corn dam...IJEABJ
Corn (Zea mays L.) is one of the most important cereals in Côte d'Ivoire. However, Corn seeds and seedlings are susceptible to infection by a number of soilborne fungi which caused seeds decay before or after germination. The objective of this study is to evaluate the suppressive effect of Four plants compost teas (Chromolaena odorata, Ricinus communis, Nicotiana tabacum, Azadiracta indica) on Corn damping off. In vitro assays showed a most suppressive effect of C. odorata and R. communis compost teas on mycelial growth of pathogenic fungi. Results of in vivo trials showed significant reduction of Corn seedlings diseases incidence and high seed germination percent after treatment with C. odorata, R. communis and A. indica compost teas. No efficiency effect was noted with N. tabacum compost tea. This study demonstrated the usefulness of compost tea as an efficient biological tool for the control of fungi responsible of corn damping-off.
Isolation, identification of antagonistic rhizobacterial strains obtained fro...Shazia Shahzaman
Plant growth promoting rhizobacteria (PGPR), are associated with roots, found in the rhizosphere and can directly or indirectly enhance the plant growth. In this study soil was collected from rhizosphere of chickpea fields of different areas of Rawalpindi division of Pakistan. PGPR were isolated, screened and characterized. Eight isolates of rhizobacteria (RHA, RPG, RFJ, RC, RTR, RT and RK) were isolated from Rawalpindi division and were characterized. The antagonistic activity of these PGPR isolates against root infecting fungi (Fusarium oxysporum and Verticillium spp.,) was done and production of indole acetic acid (IAA), siderophore and P-solubilization was evaluated. The isolates RHA, RPG, RFJ, RC, RRD and RT were found to be positive in producing siderophore, IAA and P-solubilization. Furthermore, most of the isolates showed antifungal activity against Fusarium oxysporum, and Verticillium spp. The rhizobacterial isolates RHA, RPG, RFJ, RC, RRD, RTR, RT and RK were used as bio-inoculants that might be beneficial for chickpea cultivation as the rhizobacterial isolates possessed the plant growth promoting characters i.e. siderophore, IAA production, phosphate solubilization. In in vitro tests, Pseudomonas sp. and Bacillus spp. inhibited the mycelial growth of the fungal root pathogens. The isolates (RHA and RPG) also significantly increased (60-70%) seed germination, shoot length, root length of the chickpea. The incidence of fungi was reduced by the colonization of RHA and RPG which enhanced the seedling vigor index and seed germination. The observations revealed that isolates RHA and RPG is quite effective to reduce the fungal root infection in greenhouse, and also increases seed yields significantly. These rhizobacterial isolates appear to be efficient yield increasing as well as effective biocontrol agent against fungal root pathogen.
Detection of Slime-Producing Staphylococcus aureus Strains Isolated from Food...Agriculture Journal IJOEAR
Abstract— The contamination of food with pathogenic microorganisms producing biofilm, implies a high cost for the food industry and represents a serious risk for the health of consumers. The antibacterial activity of organic extracts of Azorella trifurcata and Mulinum echegarayii was evaluated against 4 Staphylococcus aureus slime-producing strains isolated from bakery foods and against S. aureus ATCC 35556 slime-producing strain and S. aureus ATCC 25923 non slime-producing strain. The plant extracts showed antibacterial effectiveness against all the strains of S. aureus tested with minimum inhibitory concentration (MIC) between 500 and 8000 µg/ml. M. echegarayii 30:70% AcOEt:HEX showed the best activity: five strains of S. aureus showed MIC of 1000 μg/ml and S. aureus ATCC 25923 was inhibited at doses of 500 μg/ml. The values of minimum bactericidal concentration (MBC) of the extracts assayed were one or two times higher than corresponding MIC values. This study showed that extracts of Azorella trifurcata and Mulinum echegarayii are promising for future natural therapy against slime-producing S. aureus. Plant extracts with activity against slime producing S. aureus strains could provide benefits for of food technology and public health.
Prevalence, occurrence and biochemical characterization of Xanthomonas campes...INNS PUBNET
Xanthomonas campestris pv. vesicatoria the causal organism of bacterial spot in tomato results in heavy losses both in the form of quality and. In this study a survey was carried out to report the incidence of bacterial spot disease of tomato in district Swat. We reported maximum disease incidence in tehsil Kabal (71.66%), followed by Charbagh (61.66%) and Barikot (58.33%). For resistant screening a total of 13 tomato germplasms were screened against the disease. The foliar severity ranged from 3.33% to 73.33%, while severity for fruits was ranged from 18.33% to 30.66%. In case of phenotypic data the highest numbers of fruits obtained were 34, plant height 79.5cm and fruit weight was 470 grams/ten tomatoes. While the lowest average numbers of fruits were 6.67, plant height 45.7cm and fruit weight recorded was 215.67 grams/ten tomatoes. Line 1288 showed highest level of resistance followed by Red-stone. However, line 9708 showed highest susceptibility when exposed to artificial inoculation. Our study showed that bacterial spot is a major issue in some part of Pakistan and germplasm screening are linked to increased host resistance and could offer an important contribution to future integrated bacterial spot management programs.
Assessment of Endophytic Fungal Flora Responsible for Plant Growth Promotion...Sryahwa Publications
The present paper discusses the highest colonization of fungal endophytes as Alternaria speciesin comparison with Colletotrichumspecies and Fusarium species in all three plants Pongamia pinnata, Securinega leucopyrus and Rhus mysorensis. These endophytic fungi protect these plants from various
environmental factors such as temperature, moisture and other environmental factors.
SYNERGISTIC ANTIMICROBIAL ACTIVITIES OF PHYTOESTROGENS IN CRUDE EXTRACTS OF T...lukeman Joseph Ade shittu
Intensive studies on extracts and biologically active compounds isolated from medicinal plants have doubled in the last decade worldwide. However, as a result of paucity of knowledge and folkloric claim on the effectiveness of sesame leaves in infectious disease treatments, we aimed to determine the synergistic antimicrobial activity of essential oils and lignans present in the crude leaves extracts of Sesame radiatum and Sesame indicum. Ethanolic, methanolic and aqueous extracts of both leaves were studied for their in-vitro synergistic antimicrobial activity against both Gram positive and Gram negative micro-organisms, and Yeast using Agar diffusion method. The GC-MS phytochemical screening of methanolic extract showed that the major compounds in essential oils are of carboxylic acids and phenolic groups especially, the most potent antioxidants known to man like sesamol, sesamolin and sesamin among others. Methanolic and ethanolic extracts have broad spectrum antimicrobial effect against all the tested pathogenic micro-organisms except Streptococcus pneumoniae and Staphylococcus aureus respectively, while the aqueous extract exhibited inhibitory activity on Staphylococcus aureus, Streptococcus pneumoniae and Candida albicans. The result confirmed the folkloric claims of the antimicrobial effectiveness of locally consumed sesame leaves extracts especially against bacterial and common skin infection in many areas of Nigeria .
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online
Colletotrichum causes anthracnose in crops around the world producing postharvest losses up to 60%. There are a great variety of Colletotrichum strains isolated from mango orchards. Thus, it is important to characterize their pathogenicity, as well as to perform a correct identification, in order to implement good strategies to eradicate the produced disease. The aim of this work is to identify Colletotrichum spp. and to determine the production of Pectate Lyase (PL) as a virulence factor in the pathogenicity process. Macroscopic characteristics of isolated colony vary from grey to salmon, sometimes showing luxuriant orange conidial masses with grey or white bottom. Conidia vary from 10.39 to 14.83 × 2.75 to 3.40 μm corresponding to C. gloeosporioides or C. acutatum according to Sutton. Growth rates vary from 0.1948 to 0.2239 day-1. The pectate lyase activity was induced by mango cells (240.81 VS 398U/L). According to CgInt and ITS4 PCR amplification M2V and SA correspond to C. gloeosporioides.
Efficacy of insecticides against citrus psylla diaphorina citri kuwayama in f...Muhammad Qasim
The experiments were conducted in a citrus orchard to check the efficacy of insecticides against citrus psylla, and mortality was observed after three days, seven days and then after one month. Four insecticides, Polytrin-C, Talstar, Bifenthrin and Imidacloprid applied, had an almost equal effect on the population reduction of citrus psylla on all citrus plants. The trial was laid out in randomized complete block design (RCBD) having five treatments with three replications in a citrus orchard, after three days of spray showed percentage control as 96.91%, 94.33%, 93.83% and 93.06% of following insecticides Polytrin- C, Imidacloprid, Bifenthrin and Actara, respectively, calculated by Minitab 15. Psylla adults were exposed to different concentrations (500, 400, 300, 200 and 100 ppm) of Imidacloprid and Bifenthrin, and two controlled conditions (with leaves and without leaves). Both Imidacloprid and Bifenthrin insecticides proved to be the most effective against D. citri with lethal times (LT50s) of 4 and 5 hours, respectively, at a concentration of 500 ppm, calculated from probability test with Minitab-15
Effect of interaction between different plant growth regulators on in vitro s...Agriculture Journal IJOEAR
— In this paper a shoot multiplication is described for Citrus latifolia Tan. (persian lime) using nodal segment explants of young one – old – year trees by two different pathways contain with and without callusing phase. The best result for multiple shoot formation and regenerated shoot formation was 3.2 and 2.6 shoots per explants with 4.44 µM BA plus 0.053 µM NAA and 4.44 µM BA plus 0.049 µM IBA respectively. Alike shoot regeneration, shoot elongation was occurred in medium with 4.44 µM BA and 0.049 µM IBA. Micropropagated and regenerated plants are under other experiments. Abbreviation: BA – 6 benzylaminopurine; IBA – Indole acetic acid; NAA – Naphtalene acetic acid; PGRs – Plant Growth Regulators.
CHARACTERIZATION OF STREPTOMYCES SCABIES ISOLATESijabjournal
Potato, (Solanum tuberosum L,) have various biotic constraints in its production due to pest attack. Among these, common scab caused by streptomyces scabies in an important disease in potato which causes economic loss with respect to plant yield and quality of tubers. The present study was conducted to determine the pathogenicity, pathogenic variation, characterization of morphological, physiological and
biochemical aspects of Streptomyces specie associated with potato tubers grown in Rawalpindi district.Severity data and pathogenic variation of disease was studied by using different isolation and characterization techniques. Isolation and characterization of Streptomyces spp. From potato tubers will
guide the researchers about the causative strains of common scab of potato present in the particular area.
Antibacterial and Antifungal Activities of Essential Oils of Crude Extracts o...lukeman Joseph Ade shittu
Concern about the rising prevalence of antibiotics-resistant strains of pathogenic microorganisms has been expressed in the last three decades. However, intensive studies on extracts and biologically-active compounds isolated from medicinal plants have also doubled in the last decade. As a result of paucity of knowledge and folkloric claim on the leaves effectiveness in infectious disease treatments, we aimed to determine the antimicrobial activity of essential oils and lignans present in the crude Sesame radiatum leaves extracts. Ethanolic, Methanolic and Aqueous extracts of Sesame radiatum leaves were studied for their in-vitro antimicrobial activity against both Gram positive and Gram negative micro-organisms and Yeast using Agar diffusion method. The GC-MS phytochemical screening of methanolic extract showed the presence of carboxylic acids and phenolic groups in essential oils especially some of the most potent antioxidants like Sesamol, Sesamolin and Sesamin. Both the methanolic and ethanolic extracts have broad spectrum antimicrobial effect against all the tested micro-organisms except Streptococcus pneumoniae, Candida albicans and Staphylococcus aureus respectively, while the aqueous extract exhibited no inhibitory effect on Staphylococcus aureus and Streptococcus pneumoniae except on Candida albicans. The result confirmed the folkloric claims of the antimicrobial effectiveness of locally consumed Sesame leaves extracts especially against bacterial and common skin infection in many areas of the Country (Nigeria).
Synergistic antibacterial effects of three edible plants extract against anti...Open Access Research Paper
In vitro synergistic antibacterial effects among Alocasia macrorrhizos rhizome, Amorphophallus paeoniifolius corm and Colocasia esculenta corm extracts were tested against six resistant bacteria viz., Yersinia enterocolitica, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Clostridium difficile and Staphylococcus aureus. The inhibition zone was compared with the commercially available antibiotic (tetracycline). High inhibitory activity was observed against E. coli (12.67±0.33 mm) and S. aureus (12.50±0.29 mm) for methanol extract at 800 mgml-1 of concentration. MIC and MBC of the extracts ranged from 200-580 mgml-1 and 250-650 mgml-1 respectively. The lowest MIC and MBC of the extracts were measured against E. coli.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
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(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
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In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
This pdf is about the Schizophrenia.
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What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
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Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
insect taxonomy importance systematics and classification
Synopsis Seminar
1.
2. DEPARTMENT OF ENTOMOLOGY
DR. RAJENDRAPRASADCENTRALAGRICULTURAL UNIVERSITYPUSA, SAMASTIPUR
BIHAR– 848125
ProposedTopic
Studies on Various Factors of Resistance in
Maize Plants Affecting Spotted Stem Borer, Chilo
partellus (Swinhoe) Incidence.
Speaker
Reg. No.
Major advisor
:Manish Kumar Yadav
:Ph.D. (Ag.) Entomology
:D/Ento./107/2016-17
:Dr. A.K. Rai, Associate
Professor
Dept of Entomology
3. INTRODUCTION
Maize (Zea mays L.) is a third most important
cereal after rice and wheat.
Maize serves as staple food, raw material for
processed food industries and animal feed and
fodder for many countries throughout the
world
The average maize productivity in India is 2.5
t/ha which is less as compared to U.S. (9.6 t/ha)
due to less mechanization and loss of yield in
fields by insect-pests and pathogens.
4. CONT…
Maize is subjected to attack by a number of
insect-pests in which spotted stem borer is
major cause of damage during kharif season.
5. CONT.…
Spotted stem borer Chilo partellus (Swinhoe)
is a major Lepidopteran pest of maize belong
to family Crambidae.
Spotted stem borer lay eggs on leaves in a
batch of 20-50 eggs, generally close to the
midrib.
It cause serious damage in maize crop during
Kharif season by making dead hearts and
Tunnels.
Yield loss due to this pest ranges from 10 to 20
per cent and may go up to 80 per cent
(Rahman, 1994).
6. WHY WE CHOSES THIS CROP
Maize is an important cereal grown
throughout the year.
It is a major crop of Bihar, grown in large
areas.
Maize has ability to produce yield in all types
of soil and environment.
It is grown for many purposes and provide
raw materials to many industries.
7. Objectives of investigation
Physical, biochemicals and genotypic factors
are largely responsible for imparting resistance
into the maize plant against insects including
Chilo partellus (Swinhoe). The biochemical like
chlorophyll and proteins are present in maize
plant with significant role in insect-pest
resistance. Therefore, the study of the relation
of physical, biochemical and genotypic
resistance factors of maize against maize
spotted stem borer, Chilo partellus (Swinhoe) is
proposed with the following objectives.
8. 1. To assess the molecular diversity in maize
cultivars and establish correlation with different
degree of infestation of spotted stem borer, C.
partellus (Swinhoe),
2. quantitative estimation of total nitrogen,
potassium and other plant biochemical like total
chlorophyll and protein in maize cultivars and to
find out their correlation with C. partellus
(Swinhoe) infestation levels and
3. to study the physical resistance factors of
maize plant against spotted stem borer, C.
partellus (Swinhoe)
9. TECHNICAL PROGRAMME OF THE WORK
All cultivars, will be sown during Kharif in the
month of August (first week) at research farm,
DRPCAU Pusa. Test insect i. e. Chilo partellus
will be reared in laboratory and neonate larvae
will be used for artificial infestation in the field.
10. Crop : Maize
Genotypes : DRPCAU Cultivars: RHM-1, RHM-2, RHM-
3,SM-1, SM-2, SM-3, SM-4, SM-5, Dewaki,
Lakshmi, Suwan, Hemant and Deep Jwala
Monsanto: 900M Gold, 9144 SC, 7074 SC
and Double- DC
Pioneer: P-3322, P-3335 and P-3535
Pipeline Cross of DRPCAU: 12 New Cross,
13 New Cross, 14 New Cross and 15 New
Cross
Total 26 genotypes
Location : Research Farm, DRPCAU Pusa
Row length : 4 m
Row to Row Space : 60 cm
Plant to Plant Space : 20 cm
Total rows for one
genotype
: 2
Layout of the experiment
11. 1. To assess the molecular diversity in maize cultivars and
establish correlation with different degree of infestation of
spotted stem borer, C. partellus (Swinhoe).
Assessment of molecular diversity of maize cultivars will be
done by SSR marker at 35 DAS. For this experiment, DNA
will be extracted from plant samples by CTAB method
described by Doyle and Doyle, 1987. After DNA extraction,
PCR reactions will be carried out in a 20ml volume
containing 50 ng genomic DNA×PCR buffer and primers.
The amplification consist denaturing step of 2 min at 94oC.
Amplification products will be separated by electrophoresis
in horizontal gel system at 110V for 2.0 hour in 3% agarose
gel. Finally, the gels will be photographed under UV light
attached to a gel documentation system with Biocapture
software and the bands will be binary coded for each
genotype.
12. The following data will be recorded at 35 DAS.
Different injury symptoms on plants created by C.
partellus will be recorded by screening (Guthrie et.
al., 1960).
Average score of results (infestation) will be
compared with molecular diversity which will show
the different degree of susceptibility of different
genotypes against spotted stem borer, C. partellus.
13. 2. Quantitative Estimation of total nitrogen,
potassium and other plant biochemicals like total
chlorophyll and protein in maize plants and to
find out their correlation with C. partellus
(Swinhoe) infestation levels.
Quantification of above components will be
done at 35 DAS and just before start of
tasseling. The correlation between infestation
and plant nutrients will be established.
Sampling of leaves will be done in all plants of
each genotypes to form a single composite
sample. The following observations will be
recorded:
14. Nitrogen estimation: By Kjeldahl method, The
leaf sample will be heated with sulphuric acid,
which decomposes the organic substance by
oxidation. In this step, potassium sulphate will
be added to increase the boiling point of the
medium. Then the solution will be distilled with
a small quantity of sodium hydroxide, which
converts the ammonium salt to ammonia. The
amount of nitrogen present in the sample, is
determined by titration. (AOAC, 1970)
15. Potassium estimation: For calculation of
potassium, 5gm plant material will be taken
and placed in a 100 ml flask with 10 ml
digestion acid in the ratio of 9:4
(HNO3:HClO4). Then the flask will be placed
on low heat hot plate in digestion chamber
and heated upto a higher temperature to
reduce total volume (3-5 ml) by evaporation.
The composition of digestion will be
confirmed when the liquid will become
colourless and then the samples will be read in
flame photometer. (Amjad et al., 2015)
16. Estimation of total chlorophyll: 0.5g of fresh
plant leaf sample will be taken, and
homogenized in tissue homogenizer with 10 ml of
extractant solvent like 80% acetone or 95%
ethanol. Then the sample mixture will be
centrifuged at 4000 rpm for 20 min at 40⁰C.
Then the solution mixture will be analyzed for
total chlorophyll content in spectrophotometer.
The readings will be taken at 645 and 663 nm.
17. Protein estimation: Total protein content in
each genotype will be calculated by the given
formula (Winkleman et al., (1986).
Protein per cent = Nitrogen per cent×6.25
18. 3. To study the physical resistance factors of maize
plant against spotted stem borer, C. partellus
(Swinhoe)
Physical attributes of plant like plant architect,
stem diameter, plant height, internode length and
number of nodes will be recorded just before start
of tasseling in all grown genotypes.
Study of Trichome density on leaf surface will be
done by selecting and cutting an area of 2.5 cm
squire at 3 different points of a leaf at just before
tasseling stage. All the Leaves from middle point to
whorl will be selected. The counting of leaf
trichomes density will be done with the help of
ocular.
19. The associated effect of leaf trichomes i. e.,
viral and bacterial infection on larvae due to
trichome injury, will be assessed by walking the
neonate larvae from mid-point of the leaf to
stem-leaf junction on the upper surface of
matured leaf. This experiment will be
conducted just before tasseling. Observations
(Larval mortality and Tunnel length) will be
recorded by cutting and splitting stem of 10
plants from each genotypes on each time (10,
15, 20 and 25 days after release of the larvae).
20. Crop : Maize
Genotypes : All 26 previously Selected genotypes
Sowing Time : August (First week)
Location : Research Farm, DRPCAU Pusa
Row length : 4 m
Row to Row Space : 60 cm
Plant to Plant Space : 20 cm
Total rows for one cultivar : 2
Area : 153.00 2
Experimental layout for Trichome injury and its associatedeffect study:
21. Stem diameter at three height points of a
plant, plant height and number of nodes and
length will be recorded by counting and with
the help of measuring tape just before start of
tasseling (Amjad et al., 2015)
Plant architect study will be done by
measuring leaves angle in respect of stem. It
will be done with the help of protactor.