CTAB is used in DNA extraction to simultaneously solubilize plant cell walls and membranes while denaturing proteins. This prevents DNA degradation during isolation and yields highly intact genomic DNA. Chloroform helps separate proteins and polysaccharides from nucleic acids by binding them, allowing DNA to be isolated in the upper aqueous phase. Isopropanol precipitates DNA from large solution volumes at room temperature to avoid coprecipitating other molecules. Chilled ethanol causes DNA to precipitate out of solution, allowing it to be purified for genetic testing. Deionized water is used to dissolve and store extracted DNA for subsequent experiments.