Semi-natural grasslands (SNG) in Europe provide agricultural, biodiversity, and ecosystem services but their extent and conservation status is uncertain due to inconsistent definitions. SNG can support livestock production through forage production and quality, though output varies depending on plant and livestock species. Lambs grazing different SNG habitats produced meat with varying fatty acid profiles and quality attributes. SNG deliver provisioning, supporting, regulating, and cultural ecosystem services including food, nutrient cycling, climate regulation, and recreation. Their future role will depend on challenges like climate change, food security, and rural land use policies that influence agricultural management and conservation of these multi-functional landscapes.