This document discusses the properties and production of recombinant vaccines. It notes that recombinant vaccines are produced using recombinant DNA technology by inserting DNA encoding an antigen into cells to express and purify the antigen. The first approved recombinant vaccine was for hepatitis B. The document outlines the types of recombinant vaccines and the process of recombinant DNA technology, including isolating genetic material, amplifying genes of interest, and inserting recombinant DNA into host cells to produce the foreign gene product. It lists some advantages as being able to more quickly produce recombinant vaccines in larger quantities without infectious particles, making them safer for immunosuppressed individuals.

21. PROPERTIES OF A GOOD
Recombinant VACCINE
 Ability to elicit the appropriate immune response for
a particular pathogen.
 Long term protection
 Safety
 Stability
 inexpensiveness

22. RECOMBINANT VACCINE
 A recombinant vaccine is a vaccine is produced by
r-DNA technology.
 This involves inserting the DNA encoding an antigen
that stimulates an immune response into
mammalian cells or bacterial cells ;expressing the
antigen in these cells and purifying it from them.
 First recombinant vaccine –Hepatitis B vaccine

23. Types of Recombinant
vaccines
 Subunit recombinant vaccines – includes proteins, peptides and dna
 Attenuated recombinant vaccines –these genetically modified
pathogenic organism(bact. , virus) that are made of non pathogenic
and use as vaccines.
 Vector recombinant vaccines- these are genetically modified viral
vectors that van be used as vaccines against certain pathogens.

24. Processing of recombinant DNA
Technology
 Isolation of the genetic material
 Cutting of DNA at Specific location
 Amplification of Gene of interest using PCR
 Inserting of Recombinant DNA into the Host
cell/organism
 Obtaining the foreign gene product

25. HOW DNA VACCINE IS MADE
Viral gene
Expression
plasmid
Plasmid with foreign gene
Recombinant DNA
Technology

27. Bacterial cell
Transform into
bacterial cell
Plasmid
DNA

28. Plasmid DNA get
Amplified

29. Plasmid DNA
Purified
Ready to use

30. Advantages of the recombinant
vaccine
 Produced more quickly
 In larger quantities
 Free from infectious viral particles.
 They can safely be given to immunosuppressed
animal.
 They are less likely to induce side effects