Protein targeting is the process by which proteins are directed to specific locations after synthesis. Signal sequences on proteins guide their transport, as discovered by Gunter Blobel who won the Nobel Prize for this work. Protein targeting is important because proteins must be sorted to the correct locations, like organelles, to properly function. Eukaryotic protein targeting is complex due to many intracellular compartments and can involve posttranslational modifications and receptor-mediated transport mechanisms. Correct protein sorting is vital for cell function and errors can cause disease.

1. Presented by:-
Ajay kr. Chandra
BT/02/09
5th semester

2. •What is Protein Targeting?
•Why protein is targeted?
•Where it occurs?

4. signal sequences
• First by Gunter Blobel (1970) ,won the Nobel Prize (1999) for his
work on protein targeting.
• Directs a protein to its appropriate location after synthesis.
• Signals vary from 13 to 36 amino acid residues.
a. About 10 to 15 hydrophobic amino acid residues.
b. One or more positively charged residues near N-terminus.
c. A short cleavage sequence often including(polar A.A) Ala.

5. Posttranslational Modification of
Eukaryotic Proteins Begins in the ER.

6. Glycosylation Plays a Key Role in
Protein Targeting.

7. Golgybody & Lysosomal targeting.
Phosphorylation of a mannose residue on
lysosome-targeted enzymes.

8. Proteins are Targeted to Mitochondria
and Chloroplasts by Similar Pathways.

9. Nuclear Targeting.

10. Bacteria Also use Signal Sequences for
protein targeting.

11. Model for protein export in bacteria.

12. Cells Import Proteins by Receptor-
mediated Endocytosis.
clathrin

13. Summary• Protein targeting is necessary for proteins that are destined
to work outside the cytoplasm.
• Requires sorting signals that direct their transport from the
cytosol.
• Correct sorting is crucial for the cell; errors can lead to
diseases.
• Protein targeting is more complex in eukaryotes because of
the presence of many intracellular compartments.
• targeting either of two ways depending on the protein ….
1) Co translational translocation .
2) Posttranslational translocation .

14. References
• Wikipedia… www.google.co.in.
• LEHNINGER :- principles of Biochemistry ; Cox and Nelson ;
5th edition ; page no.= 1100-1107.
• Molecular cell Biology:- Lodish and Berk et al ; 5th edition ;
page no.= 657-739.

15. Thanks