Protein engineering
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This document discusses protein engineering, which uses recombinant DNA technology to modify protein structure and function. It describes several methods of protein engineering, including site-directed mutagenesis, error-prone PCR, and DNA shuffling. The objectives of protein engineering are to improve protein stability, modify cofactor requirements, increase enzyme activity, and modify enzyme specificity. As an example, the document discusses how site-directed mutagenesis was used to increase the stability of streptokinase by replacing lysine residues susceptible to cleavage by plasmin.
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Protein Engineering
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Protein Engineering - Ashvini.pptx
Protein engineering is the process of developing useful or valuable proteins.
Protein Engineering is a second generation of recombinant DNA technology.
It involves altering cloned DNA in vitro by novel mutational technique so that translated proteins have slightly altered properties.
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Objective:
To create a superior enzymes to catalyze the production of high value specific chemicals.
To produce enzyme in large quantities.
Eliminate the need for co factor in enzymatic reaction.
Change substrate binding sites to increase specificity.
Change the thermal tolerance and pH stability.
Increase protein resistance to proteases.
To produce biological compounds.
Investigate how desired mutations can be introduced into a cloned gene
Protein engineering
This document discusses protein engineering and methods to improve protein stability. It begins by defining protein engineering as modifying protein structure using recombinant DNA technology or chemical treatment to achieve desirable functions. The objectives of protein engineering are outlined, such as creating superior enzymes for industrial use. Methods of protein engineering discussed include mutagenesis and gene modification techniques. Strategies to increase protein stability through additions like disulfide bonds or changes to amino acids are also presented. The document provides examples of protein engineering applications in medicine, industry and agriculture.
Protein engineering
Protein engineering is the process of developing useful or valuable proteins. It is a young discipline, with much research taking place into the understanding of protein folding and recognition for protein design principles
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Protein engineering involves modifying protein structure using recombinant DNA technology or chemical treatment to improve function for use in medicine, industry, and agriculture. The objectives of protein engineering are to create superior enzymes for specific chemical production, produce enzymes in large quantities, and produce superior biological compounds. Protein engineering aims to alter properties like kinetic properties, thermostability, stability in nonaqueous solvents, substrate specificity, and cofactor requirements to meet industrial needs. Common methods for protein engineering include mutagenesis, selection, and recombinant DNA technology.
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This document discusses various techniques for protein engineering and site-directed mutagenesis. It describes altering the sequence of proteins through genetic engineering to improve properties like stability and changing amino acids near the active site to modify enzyme specificity. Directed evolution and DNA shuffling techniques are also discussed that introduce mutations and recombine protein domains to generate novel proteins with optimized functions.
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Protein Engineering
It describes the Rational and Random approaches of protein engineering and various methods of modifications in protein structure.
Protein Engineering - Ashvini.pptx
Protein engineering is the process of developing useful or valuable proteins.
Protein Engineering is a second generation of recombinant DNA technology.
It involves altering cloned DNA in vitro by novel mutational technique so that translated proteins have slightly altered properties.
Protein engineering and its techniques himanshu
b pharma 6th sem
pharmaceutical biotechnology
Protein engineering
Objectives of protein engineering
Rationale of protein engineering
Protein engineering methods
Rational design -site-directed mutagenesis methods
Advantages and disadvantages of rational design
Directed evolution -random mutagenesis
Advantages and disadvantages of directed evolution
Peptidomimetics
Classification of peptidomimetics
Advantages and disadvantages of peptidomimetics
Flow cytometry
Instrumentation
Principle
components
Protein engineering
Objective:
To create a superior enzymes to catalyze the production of high value specific chemicals.
To produce enzyme in large quantities.
Eliminate the need for co factor in enzymatic reaction.
Change substrate binding sites to increase specificity.
Change the thermal tolerance and pH stability.
Increase protein resistance to proteases.
To produce biological compounds.
Investigate how desired mutations can be introduced into a cloned gene
Protein engineering
This document discusses protein engineering and methods to improve protein stability. It begins by defining protein engineering as modifying protein structure using recombinant DNA technology or chemical treatment to achieve desirable functions. The objectives of protein engineering are outlined, such as creating superior enzymes for industrial use. Methods of protein engineering discussed include mutagenesis and gene modification techniques. Strategies to increase protein stability through additions like disulfide bonds or changes to amino acids are also presented. The document provides examples of protein engineering applications in medicine, industry and agriculture.
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Protein engineering is the process of developing useful or valuable proteins. It is a young discipline, with much research taking place into the understanding of protein folding and recognition for protein design principles
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Protein engineering involves modifying protein structure using recombinant DNA technology or chemical treatment to improve function for use in medicine, industry, and agriculture. The objectives of protein engineering are to create superior enzymes for specific chemical production, produce enzymes in large quantities, and produce superior biological compounds. Protein engineering aims to alter properties like kinetic properties, thermostability, stability in nonaqueous solvents, substrate specificity, and cofactor requirements to meet industrial needs. Common methods for protein engineering include mutagenesis, selection, and recombinant DNA technology.
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This document discusses various techniques for protein engineering and site-directed mutagenesis. It describes altering the sequence of proteins through genetic engineering to improve properties like stability and changing amino acids near the active site to modify enzyme specificity. Directed evolution and DNA shuffling techniques are also discussed that introduce mutations and recombine protein domains to generate novel proteins with optimized functions.
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This document discusses protein engineering techniques for modifying proteins, including rational protein design using site-directed mutagenesis and directed evolution using random mutagenesis. Site-directed mutagenesis involves introducing point mutations in a particular known area to modify a specific protein function, while directed evolution generates genetic diversity through random mutagenesis and screens variants to identify successful mutations without requiring structural information. Common random mutagenesis methods discussed are error-prone PCR and DNA shuffling, which can be used to engineer properties like protein folding, stability, binding, and catalysis.
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Useful in treating some human genetic disorders – Hemophilia, Duchenne Muscular Dystrophy.
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This document provides an outline for a presentation on directed evolution. It discusses the process of directed evolution, which involves randomly introducing mutations at the genetic level followed by selection of variants with desired protein characteristics. The document also covers types of mutations, naturally evolutionary processes like random mutagenesis and gene recombination that directed evolution mimics, library size, selection and screening strategies, applications, and advantages of directed evolution over rational design.
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The document discusses protein engineering and techniques used for it. Protein engineering involves altering cloned DNA to modify protein properties. It merges molecular biology, protein chemistry, and other disciplines. Techniques include genetic modifications like site-directed mutagenesis and chemical modifications. Site-directed mutagenesis allows specific changes to the DNA base using methods like oligonucleotide primers and PCR. This allows investigation of protein function and commercial applications like creating detergent-stable enzymes. Protein engineering has applications in increasing stability, activity and investigating protein properties.
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This document summarizes a seminar presentation on protein therapeutics. Therapeutic proteins are engineered proteins used for pharmaceutical purposes. The production of therapeutic proteins involves genetically modifying host cells to artificially synthesize proteins that treat diseases by replacing proteins the body is deficient in. Therapeutic proteins have been used since the 1920s, with human insulin being the first example. Today, therapeutic proteins are an important class of drugs, accounting for 16% of prescription drug sales, and are primarily produced through recombinant methods.
Protein engineering
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Protein engineering
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Introduction to Protein Engineering
The document provides an overview of protein engineering. It discusses the human organ system and how proteins are involved. It then covers basics of proteins and amino acids, as well as how proteins are manufactured in biological systems. The document outlines different levels of protein structure and the functions of proteins. It also summarizes the process from DNA to protein. Key aspects of protein engineering like objectives, rationale, challenges and approaches are described. The document is an introductory lecture on protein engineering.
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Traditionally, four methods are used for enzyme immobilization, namely (1) non-covalent adsorption and deposition, (2) physical entrapment, (3) covalent attachment, and (4) bio-conjugation . Support binding can be physical or chemical, involving weak or covalent bonds.
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1. Introduction
2. Therapeutic approaches
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4. Antibiotics
5. Antibodies
5. Biopharmaceuticals
6. CRISPR/Cas9 gene editing in humans
7. Organ on chip
8. Sequencing of next generation
THANK YOU
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The document discusses recombinant protein expression and engineering. It describes:
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2) Factors to consider like the protein's origin (prokaryotic/eukaryotic), required post-translational modifications, and available expression systems.
3) A case study expressing recombinant human alpha-1-acid glycoprotein in E. coli, including vector construction, periplasmic extraction, affinity purification, and yield.
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Protein engineering involves modifying protein structure using recombinant DNA technology or chemical treatment to produce desirable functions. The objectives of protein engineering are to create superior enzymes for specific applications, produce enzymes and biological compounds in large quantities, and create compounds superior to natural ones. Protein engineering aims to alter properties like kinetic properties, thermostability, pH and temperature tolerance, substrate specificity, and molecular weight to optimize enzymes for industrial uses. Key methods for protein engineering include mutagenesis, gene modification using oligonucleotides or de novo gene synthesis, and chemical modification like PEG conjugation. Several proteins have been successfully engineered including insulin and cytochrome c.
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Protein engineering involves modifying protein structure using recombinant DNA technology or chemical treatment to achieve a desired function. The objectives of protein engineering are to create superior enzymes for producing specific chemicals, produce enzymes in large quantities, and produce biological compounds superior to natural ones. Key properties that can be engineered include kinetic properties, thermostability, stability in nonaqueous solvents, substrate specificity, cofactor requirements, optimum pH, molecular weight, and subunit structure. Common protein engineering methods are mutagenesis, selection, and using recombinant DNA technology.
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Mule event processing models | MuleSoft Mysore Meetup #47
Mule event processing models | MuleSoft Mysore Meetup #47
Event Link:- https://meetups.mulesoft.com/events/details/mulesoft-mysore-presents-mule-event-processing-models/
Agenda
● What is event processing in MuleSoft?
● Types of event processing models in Mule 4
● Distinction between the reactive, parallel, blocking & non-blocking processing
For Upcoming Meetups Join Mysore Meetup Group - https://meetups.mulesoft.com/mysore/YouTube:- youtube.com/@mulesoftmysore
Mysore WhatsApp group:- https://chat.whatsapp.com/EhqtHtCC75vCAX7gaO842N
Speaker:-
Shivani Yasaswi - https://www.linkedin.com/in/shivaniyasaswi/
Organizers:-
Shubham Chaurasia - https://www.linkedin.com/in/shubhamchaurasia1/
Giridhar Meka - https://www.linkedin.com/in/giridharmeka
Priya Shaw - https://www.linkedin.com/in/priya-shaw
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core of India. Mirzapur, with its varied terrains and abundant biodiversity, offers an optimal
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The utilization of land is impacted by human needs and environmental factors. In countries
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Therefore, human intervention has significantly influenced land use patterns over many
centuries, evolving its structure over time and space. In the present era, these changes have
accelerated due to factors such as agriculture and urbanization. Information regarding land use and
cover is essential for various planning and management tasks related to the Earth's surface,
providing crucial environmental data for scientific, resource management, policy purposes, and
diverse human activities.
Accurate understanding of land use and cover is imperative for the development planning
of any area. Consequently, a wide range of professionals, including earth system scientists, land
and water managers, and urban planners, are interested in obtaining data on land use and cover
changes, conversion trends, and other related patterns. The spatial dimensions of land use and
cover support policymakers and scientists in making well-informed decisions, as alterations in
these patterns indicate shifts in economic and social conditions. Monitoring such changes with the
help of Advanced technologies like Remote Sensing and Geographic Information Systems is
crucial for coordinated efforts across different administrative levels. Advanced technologies like
Remote Sensing and Geographic Information Systems
9
Changes in vegetation cover refer to variations in the distribution, composition, and overall
structure of plant communities across different temporal and spatial scales. These changes can
occur natural.
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