This document provides procedures and recipes for making various staining reagents used in microbiology laboratories. It includes steps to make acid-fast stains, capsule stains, fungal stains, gram stains, negative stains, and spore stains. For each stain type, it lists the chemicals and amounts needed to make the staining solutions. At the end, it provides contact information for the website that offers laboratory equipment and supplies.

1. Prosedur Pembuatan
Reagent Untuk Pewarnaan .
www.AlatAlatLaboratorium.com/Blog

2. Daftar Isi
Acid Fast Stain / Pewarnaan Bakteri Tahan Asam.
Capsule Stain / Pewarnaan Kapsul.
Fungal Stain / Pewarnaan Jamur.
Gram Stain / Pewarnaan Gram.

3. Daftar Isi
Negative Stain / Pewarnaan Negatif.
Spore Stain / Pewarnaan Spora.

4. Acid-fast Stain

5. Solution A
 Basic Fuchsin (90% dye content) = 0,3 g
 Ethyl alcohol (95%) = 10,0 ml
Solution B
 Phenol = 5,0 g
 Distilled water = 95,0 ml
Note : Mix solutions A and B. Add 2 drops of trition X
per 100 ml of stain for use in heatless method

6. 

Ethyl alcohol (95%) = 97,0 ml
Hydrochloric acid = 3,0 ml

7. 

Methylene Blue = 0,3 g
Didtilled water = 100,0 ml

8. Capsule Stain

9. 

Crystal Violet (85% dye Content) = 1,0 g
Distilles Water = 100,0 ml

10. 

Copper Sulfate (CuSO₄ . 5H₂O) = 20,0 g
Distilled water = 80,0 ml

11. Fungal Stains

12. Lactic acid = 20,0 ml
 Phenol = 20,0 g
 Glycerol = 40,0 ml
 Distilled water = 20,0 ml
 Aniline Blue = 0,05 g
Note : Heat Gently in hot water (double boiler) to
dissolve; then add aniline blue dye


13. 

Gram`s iodine (as in Gram`s stain) = 10,0 ml
Distilled water = 30,0 ml

14. Gram Stain

15. Solution A
 Crystal Violet (90% dye content) = 2,0 g
 Ethyl alcohol (95%) = 20,0 ml
Solution B
 Ammonium oxalate = 0,8 g
 Destilled water = 80,0 ml
Note : mix solutions Aand B

16. 


Iodine = 1,0 g
Potassium iodide = 2,0 g
Distilled water = 300,0 ml

17. 

Ethyl Alcohol (100%) = 95,0 ml
Distilled water = 5,0 ml

18. 


Safranin O = 0,25 ml
Ethyl alcohol (95%) = 10,0 ml
Distilled water = 100,0 ml

19. Negative Stain

20. Nigrosin, water-soluble = 10,0 g
 Disrilled water = 100,0 ml
Note : Immerse in boiling water bath for 30 minutes.
 Formalin = 0,5 ml
Note : Filter twice through double filter paper


21. Spore Stain

22. 

Malacite green = 5,0 g
Distilled water = 100,0 ml

23. 


Safranin O = 0,25 ml
Ethyl alcohol (95%) = 10,0 ml
Distilled water = 100,0 ml

24. Our Website
www.AlatAlatLaboratorium.com.
Toko Online Alat Laboratorium
www.AlatAlatLaboratorium.com/Blog
Panduan Memilih Alat Laboratorium
www.AlatAlatLaboratorium.com/LaboratoriumMikrobiologi
Daftar Peralatan Pembuatan Laboratorium Mikrobiologi
www.AlatAlatLaboratorium.com/Memmert
Informasi Product Memmert di Indonesia

25. Facebook :
AlatAlatLaboratorium.
Q+A
Twitter :
Alat2Lab.
Telp/Sms :
0852-1304-3138.
E-mail :
sales@AlatAlatLaboratorium.com.

26. THANK
YOU!