Endoscopy involves using specialized cameras to evaluate areas of the body in a minimally invasive manner. It is commonly used for diagnostic purposes to visualize abnormalities and obtain biopsy samples. Some advantages of endoscopy include it being nonsurgical, allowing direct visualization and sampling, and in some cases performing therapeutic procedures. Common types of endoscopy discussed in veterinary medicine include laparoscopy, arthroscopy, cystoscopy, and rigid endoscopy of various organs.
Diagnosing and Treating Canine Incontinence and Urolithsupstatevet
Alison Khoo, BSc, BVMS, DACVIM (Internal Medicine)
Urinary incontinence is a common presenting complaint in veterinary practice. Treatment of refractory cases may become a major source of frustration for both owners and veterinarians. Medical, surgical, and interventional therapeutic options will be discussed.
Laparoscopy is an innovative diagnostic and surgical tool in veterinary field. Laparoscopic surgeries revolutionizes the minimally invasive surgical approaches with less surgical trauma and faster recovery.
Exfoliative vaginal cytology help to determine the stage of estrus, is very simple method, cost-effective and comparatively accurate test for predicting the she dogs fit for breeding.
Diagnosing and Treating Canine Incontinence and Urolithsupstatevet
Alison Khoo, BSc, BVMS, DACVIM (Internal Medicine)
Urinary incontinence is a common presenting complaint in veterinary practice. Treatment of refractory cases may become a major source of frustration for both owners and veterinarians. Medical, surgical, and interventional therapeutic options will be discussed.
Laparoscopy is an innovative diagnostic and surgical tool in veterinary field. Laparoscopic surgeries revolutionizes the minimally invasive surgical approaches with less surgical trauma and faster recovery.
Exfoliative vaginal cytology help to determine the stage of estrus, is very simple method, cost-effective and comparatively accurate test for predicting the she dogs fit for breeding.
This lecture describes the use of ultrasonography in animal reproduction. This lecture would be useful for veterinary students, practitioners, and researchers.
This lecture describes the use of ultrasonography in animal reproduction. This lecture would be useful for veterinary students, practitioners, and researchers.
Capsule Endoscopy & Motorized Spiral Enteroscopy for Small Bowel ImagingYashodaHospitals
Many conditions of the small intestine affect digestive health and appetite. Intestinal obstruction due to bleeding, malformations or injury or hernia is a major diagnostic related to these problems. See how capsule endoscopy and spiral endoscopy enables the doctors to not only diagnose but treat the condition right away.
Endoscopy: Types, Preparation, Diagnosis, Procedure and RisksYashodaHospitals
An endoscopy is a diagnostic tool. Find out what it is used for, what happens during an endoscopy, various types of procedure available and endoscopy risks.
Minimally invasive/accessed surgery comprises of robotic and non robotic surgery. Non robotic surgery includes laparoscopy, endoscopy, arthroscopy and etc.
Endoscopy is a nonsurgical procedure used to examine a person's digestive tract.
Using an endoscope, a flexible tube with a light and camera attached to it, your doctor can view pictures of your digestive tract on a color TV monitor.
Unlike many other medical imaging technique, endoscopes are inserted directly into the organ.
Laparoscopic surgery or minimally invasive surgery (MIS) has numerous advantages such as less pain , less blood loss, early recovery and shorter hospital stay.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
The increased availability of biomedical data, particularly in the public domain, offers the opportunity to better understand human health and to develop effective therapeutics for a wide range of unmet medical needs. However, data scientists remain stymied by the fact that data remain hard to find and to productively reuse because data and their metadata i) are wholly inaccessible, ii) are in non-standard or incompatible representations, iii) do not conform to community standards, and iv) have unclear or highly restricted terms and conditions that preclude legitimate reuse. These limitations require a rethink on data can be made machine and AI-ready - the key motivation behind the FAIR Guiding Principles. Concurrently, while recent efforts have explored the use of deep learning to fuse disparate data into predictive models for a wide range of biomedical applications, these models often fail even when the correct answer is already known, and fail to explain individual predictions in terms that data scientists can appreciate. These limitations suggest that new methods to produce practical artificial intelligence are still needed.
In this talk, I will discuss our work in (1) building an integrative knowledge infrastructure to prepare FAIR and "AI-ready" data and services along with (2) neurosymbolic AI methods to improve the quality of predictions and to generate plausible explanations. Attention is given to standards, platforms, and methods to wrangle knowledge into simple, but effective semantic and latent representations, and to make these available into standards-compliant and discoverable interfaces that can be used in model building, validation, and explanation. Our work, and those of others in the field, creates a baseline for building trustworthy and easy to deploy AI models in biomedicine.
Bio
Dr. Michel Dumontier is the Distinguished Professor of Data Science at Maastricht University, founder and executive director of the Institute of Data Science, and co-founder of the FAIR (Findable, Accessible, Interoperable and Reusable) data principles. His research explores socio-technological approaches for responsible discovery science, which includes collaborative multi-modal knowledge graphs, privacy-preserving distributed data mining, and AI methods for drug discovery and personalized medicine. His work is supported through the Dutch National Research Agenda, the Netherlands Organisation for Scientific Research, Horizon Europe, the European Open Science Cloud, the US National Institutes of Health, and a Marie-Curie Innovative Training Network. He is the editor-in-chief for the journal Data Science and is internationally recognized for his contributions in bioinformatics, biomedical informatics, and semantic technologies including ontologies and linked data.
Priniciples of Canine Endoscopic Surgery Prof.Dr. Awad Rizk.pdf
1. Principles of Small Animal Endoscopy
Prof. Dr. Awad Rizk
Prof. of Veterinary Surgery, Anesthesiology and Radiology
Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt
Ph.D. University of Veterinary Medicine Hannover, Germany
Guest Prof. University of Leipzig, Leipzig, Germany
Prof.Dr.Awad Rizk
2.
3. ❑Endoscopy is the use of specialized video cameras to
evaluate areas within the body in a minimally invasive
manner.
❑ In most instances, endoscopy is performed for
diagnostic purposes allowing visualization and sampling
of abnormalities.
❑Endoscopy can also be used for therapeutic purposes as
well, termed interventional endoscopy.
4. Advantage of endoscopy
Diagnostic
✓ Evaluating the digestive system is that it is nonsurgical.
✓ The technique allows for visualization of the lining of the
digestive system
✓ Direct Biopsy samples can take from organs.
✓ Many foreign bodies in the esophagus and stomach may be
removed via endoscopy.
✓ Specialized video camera with high resolution to evaluate is
now available in market for better and accurate diagnosis
5. In surgical procedure (interventional endoscopy)
✓Endoscopies are almost painless although they may
still cause some discomfort.
✓Compared with stress experienced by the body in a
full surgical procedure
✓an endoscopy is simple, low risk, and cost effective.
Other advantages include:
✓no scar – as a natural body opening is used
✓ quick recovery time
✓ less time in hospital, often, no time in hospital is
required as the procedure is performed in the
doctor’s rooms, and early detection of
postoperative re-occurrence
6. other advantages of endoscopy
✓less in morbidity and mortality
✓minimally invasive
✓can be inserted into the natural openings of the body
such as the mouth or anus.
✓ Also, they can be inserted into small incisions.
✓Its application in veterinary medicine has great role
for the diagnosis and they can be used to examine
visually or assist in surgery such as an arthroscopy
and in therapeutic procedure.
7. Disadvantage of endoscopy
✓ Necessity to give general anesthesia to the patient
✓ Adequate laboratory testing and radiology is required before
an endoscopy
✓ Evaluation of blood test before giving anesthesia is
important for confirmation of patient is ready to take
anesthetic risk or not
✓ Fasting of animals are at least 12 hours before an elective
endoscopy
✓ A mouth gag is used to prevent damage to the endoscope.
✓ If lower part is to be examine it requires fasting more than
24 hrs to 48 hrs.
✓ Enemas are important to clean the intestines
✓ Care should be required at the time of endoscopy like
tearing of intestine; perforation wound otherwise immediate
surgery is required to correct the problem.
8.
9. To avoid damage of endoscope
1. Mouth gag
2. Never force scope –avoid sharp bend
3. Inadequate anesthesia
10. Limitation of endoscopy in veterinary medicine
✓ Cannot assess functional disease luminal diameter, wall thickness
✓ Cannot identify disease in most of the small intestine
✓ Cannot detect disease in the deep submucosa, muscularis or
serosa
✓ Not appropriate if bowel perforation is suspected
✓ Not ideal if pet not adequately prepared,
✓ Cannot assess or biopsy lymph nodes, Biopsy samples are
very small, need multiple
✓ It requires experience and knowledge
✓ Requires regular cleaning
11. ➢ Endoscopy has become very important just as radiology or
ultrasonography for veterinary specialist.
➢ Endoscopy is a valuable tool to peer view into interiors of the body.
➢ A basic knowledge of the normal appearance of the various luminal
surfaces, and the underlying anatomy is essential.
➢ The use of endoscopy requires proficiency in anesthetic techniques,
patient preparation, and in-depth knowledge of luminal affections in
canines.
➢ Many gastrointestinal lesions have a polymorphic appearance, so biopsy
is mandatory for definitive diagnosis.
➢ Endoscopic surgery results in less surgical morbidity,
➢ less postoperative pain and faster recovery.
12. History of endoscopy
✓ 1902: Georg Kelling of Dresden: performed the first laparoscopic
procedure on dogs.
✓ 1910: Hans Christian Jacobaeus of Sweden: the first laparoscopic
operation on humans.
✓ 1980: Patrick Steptoe, performed laparoscopic procedures in the
operating room under sterile conditions.
✓ 1982: The first solid state camera was introduced and this was the
start of video laparoscopy.
✓ 1983- First laparoscopic appendicectomy Semm, a German
gynecologist.
✓ 1985- First Lap Cholecystectomy Erich Muhe a German surgeon. •
1987- First Laparoscopic repair of inguinal hernia by Ger.
✓ 1989- First lap hysterectomy, Reich
13. Types of Endoscopy
Flexible endoscopy:
• Bronchoscopy: an exam of the lower airways.
• Colonoscopy:an exam of the transverse colon, ascending colon, cecum, large
bowel, and rectum.
• Endoscopy: an exam of the esophagus, stomach, and upper intestines.
Rigid endoscopy:
• Arthroscopy: an exam of soft tissue structures and joint cartilage, which is
not visible on radiographs. Decreased damage to the joint and shortened
recovery times are two advantages of arthroscopy over arthrotomy (surgical
exam of the joint). Disadvantages include its limitation during diagnostic and
corrective surgical procedures in small patients.
• Cystoscopy: an examination of the vagina, urethral opening, urethra,
bladder, and ureteral openings.
• Laparoscopy: an exam of the abdominal cavity performed through a small
incision in the wall of the abdomen or through the navel. It is done in
veterinary medicine to obtain hepatic (liver) and renal (kidney) biopsy
samples.
• Proctoscopy: An exam of the large bowel and rectum.
• Rhinoscopy: an exam of the nasal cavity and nasopharynx (junction between
the nasal area and the back of the throat).
• Thoracoscopy: an examination of the chest cavity. This is currently not
performed frequently in veterinary medicine.
14.
15.
16. • Endoscopy is performed with either a rigid or
flexible fiber optic instrument.
17. • ENDOSCOPY EQUIPMENT BASICS
The basic endoscope consists of the:
Insertion tube—depend on the mechanism for image
transmission:
Fiberoptic glass bundles (fiberscope) or charge-coupled
device (CCD) chip (videoendoscope)
Biopsy/suction channel
Irrigation/insufflation channel
Deflection control cables
Handpiece—Includes the:
Deflection control knobs
Accessory channel entrance
Irrigation/insufflation
Suction valves
Umbilical cord—Responsible for light transmission
18.
19. FLEXIBLE ENDOSCOPES
• There are 2 main types of flexible endoscopes:
the fiberscope and videoendoscope.
• The key differentiating feature is the mechanism
by which an image is sensed and transmitted for
viewing.
• Flexible endoscopes are used predominately for
navigating the complex anatomy of the GI tract.
20. • Flexible endoscopes
such as those used in the examination of the
stomach consist of a long, flexible insertion tube
with a bending tip at the end that enters the
body, an eyepiece, and a control section.
21. • The tip of the endoscope is manipulated using a
control knob in the hand piece.
• In addition to the fiber bundles which provide
the light source, two channels are present within
the endoscope.
22. ❖One channel permits various endoscopic tools to
be passed and fluids to be suctioned or samples
taken.
❖The other allows air or water to be passed into the
stomach/intestine to insufflate (inject air into the
area), or wash away mucus from the viewing port.
❖Special video cameras can be attached to the
endoscopes which allow viewing of the exam on a
television screen, as well as recording the exam
on video.
23.
24.
25.
26. • The rigid endoscope cannot be used in some
areas, such as the stomach because it does not
have the bending tip, so it cannot be flexed to
allow examination of all parts of the stomach.
27. The Equipment
1. Laparoscope/video system
2. Light source
3. Insufflator
4. Diathermy /coagulation:cutting system
5. Suction irrigation system
6. Specialized hand instruments
30. The Equipment
1. Laparoscope/video system
There are two types:
• Telescopic rod lens system, that is connected to a
video camera (single chip or three chip) or
• A digital laparoscope where the charge-coupled
device[CCD] is placed at the end of the laparoscope,
eliminateing the rod lens system.
31. Telescopic rod lens system
There are three important structural differences
in telescope available in the market.
• 6 to18 rod lens system telescopes
• 0 to 120 degree telescopes
• 1.5 mm to 15 mm of telescopes
33. Video camera
Single chip VS three chip
Three primary colours (Red,Blue, Green).
In single chip camera all these 3 primary colours are
sensed by single chip.
In three chip camera there are 3 CCD- Chips for
separate capture and processing of 3 primary
colours—High resolution
36. Monitor
• No different from the T.V.
• Basic principle of image reproduction is
horizontal beam scanning on the face of the
picture tube.
37. The existing television systems in use differ
according to the country.
• The U.S.A uses the NTSC (National Television
System Committee) system.
• In European countries the PAL (Phase Alternation b
Line) system is in use.
• French system called SECAM (Sequential color and
memory).
38. Light source
A fiber optic cable system connected to a 'cold'
light source (halogen or xenon), to illuminate
the operative field,
41. Insufflator
The abdomen is insufflated with carbon dioxide gas
[pneumoperitomeum] to create a working and viewing
space.
Elevates the abdominal wall above the internal organs
like a dome.
Gasless surgery –with mechanical wall elevators
64. • The equipment used during laparoscopy includes:
an optical system
• consists of an endoscope, a light source, and a light cable.
an insufflation system
• Automatic, high-flow insufflators (10 L/min) usually are used to insufflate
the abdomen.
adapted surgical instruments
66. • Carbon dioxide (CO2) is the most widely used gas for
insufflation because:
❑ it is inexpensive
❑ is the least likely to cause gaseous emboli compared with
nitrous oxide, air, and helium.
• The primary disadvantage of CO2 ;
✓ is the production of postoperative discomfort when it turns
into carbonic acid on the moist peritoneal surfaces.
• This complication is reported frequently in people and has
been observed in horses
67. Trocar-cannula system
❑ A trocar-cannula system is used to penetrate the body wall and
allow introduction of the laparoscope and surgical instruments
into the abdominal cavity
❑ Cannulas have a lateral Luer-Lok connector for the attachment of
the insufflation tubing system
❑ They also contain a one-way valve that allows for entry and exit of
instruments with maintenance of the pneumoperitoneum
68. Disposable cannula
(A) and trocar
(B) used for laparoscopy.
Nondisposable cannula (A) and
trocar (B) used for laparoscopy
71. Veress needle (A) connected to an insufflator by a tubing system (B) is inserted through the
body wall (C) to create a pneumoperitoneum before the laparoscopic cannula is inserted
72. During a laparoscopic procedure, laparoscopic retractors (A) and bowel clamps (B) are used
to manipulate the surrounding tissue and organs away from the surgical site.
73. • When the laparoscopic examination is complete, the
endoscope is removed, and the surgeon releases the CO2
gas/air via the open cannulas
• The portal sites are not sutured until the end of the
procedure, after all gas has been evacuated;
this limits subcutaneous emphysema around the surgical sites
and allows the maximal amount of CO2 to escape and helps
prevent postoperative discomfort.
86. Surgical operations performed with laparoscopic
methods are characterized by:
✓Their low invasiveness,
✓Small percentage of complications
✓ Low mortality.
✓To shorten significantly patients’ hospitalization
time
✓ Faster recovery and entailing a higher level of
satisfaction in their owners.
87. • The most common indications to laparoscopy in
animals are:
▪ Biopsy of abdominal organs or abdominal tissue masses
▪ Surgical operations such as:
✓ Ovariohysterectomy
✓ Vasoligation
✓ nephrectomy,
✓ Cholecystectomy
✓ Cystotomy
✓ Thoracotomy
88. • Although laparoscopy is a low-invasive
procedure, not every patient can be subjected
to it.
• Laparoscopic operations should not also be
performed in obese animals, ones with
abdominal dropsy, or in general bad condition
• Pyometra is also listed as a contraindication to
laparoscopy
89.
90. ✓ Laparoscopic unit. From top to bottom:
monitor
✓ Printer
✓ Camera
✓ insufflator
✓ DVD recorder
✓ source of light
Basic elements of equipment necessary to
perform laparoscopic operations include:
❖ a laparoscope (a telescope)
❖ a trocar
❖ a source of light
❖ a Veress needle
❖ an insufflator (a laparofflator)
❖ and a video camera
94. Origins
• First laparoscopic
procedure performed in
1902 by Georg Kelling of
Dresden, Germany in a
dog.
• 1910-First laparoscopic
procedure performed in a
human by Hans Christian
Jacobaues of Sweden.
Hans Christian Jacobaeus
95. • The surface between the pubic bone and 5 cm cranially to the
umbilicus was shaved and scrubbed including 10 cm to each
side and Lidocaine 2% was infiltrated at the sites of trocars
placement.
96. Carbon Dioxide Gas and Laparoscopy
• Before the laparoscopic procedure is
performed, the abdomen is insufflated or
blown up like a balloon with carbon dioxide
gas.
• This causes elevation of the abdominal wall
above the internal organs like a dome to
create a working and viewing space.
97. Three portal sites were used:
• portal 1(primary port) for the laparoscope
• portals 2-3 (secondary portals) for surgical
instruments
98. • Portal 1 was located 2-3 cm cranial to the umblicus.
• Portals 2 and 3 were distal to the laparoscope insertion site and
4-6 cm bilaterally from the ventral midline.
• A 10mm skin incision was made at portal site 1, and then a
Veress needle inserted perpendicular to the abdominal wall.
• After an aspiration and injection test using a 5mL syringe
containing 5mL sterile saline solution to verify that the needle
was within the peritoneal cavity
• An automatic high-flow CO2 insufflator was connected to the
Veress needle and used to insufflate the abdominal cavity at the
rate of 1L/min with a pressure gradient of 12– 15 mmHg
according to the dogs' size.
99. • After complete pneumoperitoneum has been established, the
Veress needle was replaced with a 10/11mm trocar-cannula unit
• The trocar removed and the Co2 gas tube connected to the lure
lock of the cannula and the insufflators was set to maintain an
intra-abdominal pressure of 12-15mmHg during laparoscopy.
• A rigid type telescope connected to a light source.
• a high resolution one chip digital camera was then introduced
through the cannula to transmit the scene to the video monitor.
100.
101.
102.
103.
104.
105. Laparoscopic ovariectomy and OHE
• Advantages of laparoscopy over conventional surgery
include:
➢ Reduced pain from the surgical wounds – the pet is more
comfortable post-operatively
➢ Smaller surgical wounds
➢ Fewer stitches
➢ A faster return to normal activity, due to improved patient
comfort and reduced scar tissue formation
106.
107.
108. • All patients are positioned in dorsal recumbency and clipped
from xiphoid to pubis and dorsally past the level of the 13th
rib.
• A wide clip is necessary to accommodate placement of the
trans-abdominal suspension suture or weighted hook.
• The abdomen is aseptically prepared as for traditional
laparotomy.
109. • A skin incision no larger than the size of the intended cannula
is made using a surgical blade.
• The subcutaneous tissues are dissected down to the linea.
110. • The Hasson technique is performed by creating a mini-
laparotomy.
• A 5.5-mm cannula with blunt trocar is preferred.
• The size of the skin and linea incision is no larger than the
intended camera port (usually 5 mm).
• Stay sutures are placed on both sides of the linea incision to allow
traction during insertion of the blunt trocar.
111. • The trocar is inserted at an approximate 30-degree angle, towards
the right caudal abdomen, to avoid iatrogenic injury to the
spleen.
• Once the cannula is inserted, the obturator is removed and the
CO2 tubing attached.
• The abdomen is insufflated to a pressure of 10 mm Hg.
• All subsequent instrument trocars are placed under direct
laparoscopic visualization to avoid intra-abdominal visceral injury.
• A two-port laparoscopic OVE/OHE technique will be used.
112. • Dorsal recumbency is most commonly performed by
ovariectomy, because both ovarian pedicles can be visualized
without moving the patient.
• The table can also be tilted from side to side to help
manipulation and visualization
• Mechanical ventilation is suggested because of the intra-
abdominal insufflation pressure and the weight of the organs
on the diaphragm
• Vascular stapler is utilized if the ovarian pedicle is extremely
wide.
113. • If the ovarian artery and vein are larger than 3 mm, endoscopic
clips can be used.
• A Babcock forceps was inserted into the 12 mm cannula to grasp
the right ovary.
• The ovary was lifted from the surrounding tissue and brought to
the abdominal wall, which allowed percutaneous advancement
of a transabdominal suspension suture with a round needle to
maintain exposure of the ovarian pedicle.
115. Laparoscopic Assisted OHE
Laparoscopic-assisted OHE is performed similarly to laparoscopic
OVE with the exception that the caudal cannula be placed in a
more caudal location (over the uterine body)
and the uterine body is ligated extracorporeally via the caudal
port site.
Because the uterine body is ligated extracorporeally, we prefers to
use an 11.5-mm cannula in the caudal location for larger (>10 kg)
dogs.
116.
117. • The general location for the caudal cannula should be
approximately 1/3 of the distance between the umbilicus and the
pubis.
• The cranial cannula can be placed 2–3 cm cranial to the umbilicus
as for laparoscopic OVE.
• Once the cannulas have been placed, the dog is repositioned into
lateral-oblique recumbency and both ovarian pedicles ligated
and divided as described for laparoscopic OVE.
118. • Dissection of the broad ligament to approximately midway
between the ovarian pedicle and uterine body is all that is
typically required prior to elevation from the caudal cannula site.
• Once both ovarian pedicles have been divided and the broad
ligament dissected, the dog is positioned in dorsal recumbency
and the left proper ligament is grasped with the Babcock forceps
and brought to the base of the caudal cannula.
• If needed, the caudal port incision is lengthened directly over the
cannula while maintaining the grasp of the proper ligament.
119. • The cannula is then removed over the Babcock forceps and the
ovary and uterine horn extracted from the abdomen.
• The contralateral uterine horn and ovary is easily traced and
extracted similarly to the left.
• Once the uterine body has been well exposed the uterine body
can be ligated in standard fashion.
• The uterine stump is inspected for bleeding and returned to the
abdominal cavity.
• The port sites are closed according to the guidelines described for
laparoscopic OVE.
120. Complications
• Complications from laparoscopic ovariectomy and
ovariohysterectomy are rare but can include:
➢ Hemorrhage
➢ subcutaneous emphysema
➢ visceral organ injury (e.g., spleen
➢ inability to complete the procedure requiring conversion to
laparotomy, pain, seroma formation, and rarely infection.
125. What is cystoscopy?
• Cystoscopy is a procedure that allows specially trained veterinarians to look inside the
urinary bladder and the urethra (tube connecting bladder to outside body) using a
special instrument.
• Cystoscopy is endoscopy of the bladder and urethra performed with a thin lighted tube
called a cystoscope.
• This procedure is performed in a sterile manner under anesthesia in dogs and cats.
• This procedure does not require an incision as the camera, scope and
instruments are passed through the penis in males or the vulva in females.
126. When would cystoscopy be needed?
Cystoscopy could be helpful to determine the cause
of:
✓Blood in the urine
✓Urine leakage (urinary incontinence)
✓Urinary blockages
✓Complicated urinary tract infections
✓When abnormalities in the bladder have been
noted on radiographs (x-ray) or ultrasound
127. • Patient positioning in female dogs varies with the clinician's
preference.
• Most endoscopists favor right or left lateral recumbency with
the hind end as close to the end of the table as possible.
• Ventral-dorsal can also be utilized.
• Male dogs are always in lateral recumbency.
128. Cystoscopy Procedure in Dogs
• The dog is anesthetized intravenously.
• The bladder is filled with sterile saline and distended,
allowing the scope to enter.
• A flexible optic instrument will be inserted in the
urinary tract for male dogs, a rigid optical instrument
will be used on female dogs(no incision is required).
• The scope is placed through the urethra to obtain a
visual of the bladder and urethra.
• A video recording is often taken.
• Biopsies of tissues can also be taken.
• If bladder stones are found, they can then be
removed with grasping forceps.
129. • Cystourethroscopy is a technique used to gain access to the
lower genitourinary tract
(urethra, urinary bladder, ureteral orifices, vagina).
In most cases, cystourethroscopy is used as a diagnostic tool to
visually assess the lower urinary tract if routine diagnostic
evaluation (blood work, urinalysis, urine culture, radiography,
ultrasonography) does not yield a definitive diagnosis
136. ➢ A variety of cystoscopes is needed to perform transurethral
cystoscopy in both male and female dogs and cats because of
anatomic variations and marked variability in urethral
diameter.
➢ A rigid cystoscope can be used in female dogs and cats.
➢ Flexible cystoscopes are required in male dogs and cats for
complete evaluation of the urethra and urinary bladder.
137. • What is lithotripsy?
• Lithotripsy is the physical breaking of stones
formed by the body within the urinary tract of cats
and dogs.
• Lithotripsy is usually performed within the body
using a laser fiber via surgery or cystoscopy with a
shock wave applied to the stones.
• When would lithotripsy be needed?
Lithotripsy can be helpful at treating:
✓Bladder stones (in certain cases)
✓Problematic kidney stones (causing infection,
kidney damage, obstruction of urine flow)
✓Urethral stones
138. what happens once the stones are broken?
• In the bladder, the stones are broken and
removed with a small basket.
• Once kidney stones have been broken, a
specialized wand is used to suction the large
stone fragments. A stent (plastic tube) is then
placed in the ureter to ensure flow of urine from
kidney to bladder and avoid obstruction by small
pieces of stone.
141. • Arthroscopy is a technique for examining the inside of
a joint using a tiny camera
• To allow a detailed assessment of the joint in a
minimally invasive fashion
142. ➢Arthroscopy has numerous advantages over
arthrotomy for diagnosis and treatment of
joint disease.
➢Arthroscopy entails less disruption of the
periarticular soft tissue. Typically, two or three
one-centimeter long skin incisions are required
in conjunction with a 5-millimeter diameter
tunnel through the underlying soft tissue and
into the joint.
➢Decreased soft tissue disruption leads to less
pain and less chance for infection.
143. ➢In most cases, return to use of the limb is
quicker because of less surgically induced pain.
➢This is especially true when multiple joints are
involved and are operated arthroscopically
under the same anesthetic episode.
➢Examples of disease conditions where multiple
joints are commonly treated under the same
anesthetic episode include fragmented medial
coronoid process of the elbow and OCD of the
shoulder.
144. • Arthroscopy may be employed in a diagnostic,
therapeutic or combined modality.
• Using arthroscopy as an exploratory procedure
may prevent the necessity for an arthrotomy and
is an important advantage in cases where a
surgically treatable lesion is not found.
• Visualization of the joint typically is better with
arthroscopy than with an arthrotomy.
• In joints like the shoulder and elbow, arthroscopy
allows inspection of areas of the joint that is not
visible without performing multiple
arthrotomies. `
145. • In addition, the magnification that occurs with
arthroscopy in combination with the fluid medium
within the joint allows one to see joint pathology
that cannot be appreciated with an arthrotomy.
• Visualization of synovial membrane and cartilage
pathology, in particular, is better appreciated with
arthroscopy than with arthrotomy.
• With practice and development of proficiency, the
length of an arthroscopic procedure is often less
than an arthrotomy procedure.
• Cosmetic appearance of the dog is typically better
after arthroscopy compared to arthrotomy. For
some owners, cosmetic appearance is very
important.