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Good Pond Preparation
For Better Crop
Diseases in shrimpPrimary
• Poor Culture
management
Secondary
• Organic
matter
• DO
• Poor water
quality
• Poor climate
• Staff
Result
• WSSV
• Vibrio
• White gut
• White
muscle
• Stunted
growth
• Gill disease
• Unknown
mortalities
• etc
Bad pond preparation Leads to Low
survival
• Decreases pond carrying capacity
• Increase disease conditions
• So the survival goes down
Bad pond preparation leads to
10 days
40 days 70 days
PLANKTON: Early plankton crash/thick bloom
Plankton Crash and Depletion of DO
Pond bottom/ SludgeEarly Accumulation of toxic gasses
More Vibrios and White gut incidences
Microsporidians
(EHP)
• An Emerging Pathogen
• Hepatopancreatic Tubule Epithelial
Cells
• This Farms Affected By Retarded
Shrimp Growth
• Exhibited High mortality associated
with white faeces syndrome (WFS)
Causes of Brownish Gill
• Lumniscent / Vibrio bacteria
• Low Oxygen Levels
• Suspended solids
• Algal Choke/plankton crash
Viral disease more: Vectors/ carriers
Improper Pond preparation and poor Biosecurity
leads to WSSV
• Prolonged Green or brown gill conditions
• Deteriorated pond bottom
To avoid disease
Steps in Pond preparation
1 Removal of organic matter:
2 Drying: oxidation of organic matter
3 Liming: according to pH
4 Tilling: improve oxidation of nutrients and
organic matter
5 Soil enrichment: adding supplements
which are deficient in soils
For minimum period of 30-40 days prior to
every crop
Pond bottom after crop Harvest
Removal of organic matter
Removal of organic matter
• Removes anaerobic matter consist of
ammonia, H2S, Methane and other anaerobic
harmful substances present in sludge.
• Removes certain pathogens especially
Protozoans
Sludge Vs HealthPondbottom
Organic
matter(Rich in
organic
nutrients and
promotes
more
decaying
bacteria)
Waterparameter
Low DO
High bacteria
Release of
toxic gases
Repeated
plankton
crash
Shrimp
Running
Mortality
Vibrio
White gut
Gills
Drying
• Effective method of eliminating undesirable species
• oxidizes harmful chemicals especially sulphides
• facilitates mineralization of organic matter
• Decreases Carbon content
Tilling
Tilling
• Improves soil quality
• Speeds up the oxidation process
• Release of nutrients that are locked in the soil.
• Also improves the liming efficiency by mixing
Pond Drying Vs HealthPondbottom
Drying and
tilling
Oxidises soil
Cheapest
Waterparameter
Eliminates
many viral and
bacterial
pathogens of
previous crop
High bacteria
Release of
toxic gases
Shrimp
WSSV
Vibrio
Etc
Premature
Crop failure
pH
Check soil pH after every crop
According to soil pH add liming
Liming requirements (Mt/ha)
pH CaCO3 CaOH2
7 0.65 0.33
6.5 0.9 0.45
6 1.15 0.58
5.5 1.40 0.70
5 1.65 0.83
4.5 1.90 0.95
Liming Vs HealthPondbottom
Liming
Waterparameter
Kills bacteria
and protozoans
Stabilises
water pH
Promotes
plankton
Shrimp
Running
Mortality
Vibrio
White gut
Gills
Moulting
Differential of soil
Organics matter overloaded has to be removed
Differential of soil
Less organics matter
Differential of soil
No organic matter
Lined Ponds – Preparation Technique
Compacting
For Loose soils
Reduces turbidity
Gill chocking
Improves plankton production
SOIL ENRICHMENT
DRY METHOD (ON SOIL APPLICATION):
1. Rice flour 50 kg per acre.
2. Rice Bran 20 kg per acre.
3. Probiotics 2 ltrs. Per acre.
4. Minerals 2 kg per acre.
WET METHOD (WATER APPLICATION):
1. Rice flour 25 kg per acre.
2. Rice bran 10 kg per acre.
3. Jaggery 10-15 kg per acre.
4. Yeast 1 kg per acre.
 Soak in water fermentation for 24-36 Hrs.
Uses of Enrichment
• Digests sludge
• Promotes good bacteria
• Improves nutrient cycles and bioavailability of
nutrients
• Balances nutrients and improves C:N ratio
Biosecurity For Prevention of viral
Diseases
• To avoid pathogens
• To avoid unwanted species (Mysid, fishes,
molluscans etc)
• To avoid vectors(Crabs, Arthropods etc)
• To avoid contamination between farms and
ponds
Bio Security:
 Bird Fencing
 Crab Fencing
 Use good meshes at water pumping (40,60,80,100)
 Use PP water at enter into the farm and before enter to
the farm wash your hands and legs
Screening meshes Bird fence and Crab fence
Bird fences
Reservoir/chlorination for water treatment
Chemical
Concentr
ation
Minimum
withdrawal period
Chlorine
20-30
ppm
3 days with aeration
and sunlight
Dichlorovos /
Nuvon
2ppm 7 days
CuSO4 (Molluscs
and bivalves)
7ppm 7 days
Biosecurity for Aquaculture Facilities,
2009
Select Disinfectants for Aquaculture
Product Concentration* Uses and Precautions
Virkon®
Aquatic
1:100 (1%) to 1:200
(0.5%) for 1 minute
Equipment, pumps, protective clothing, foot dips,
bilges, bins, buckets, harvesting equipment, vehicles
Calcium
hypochlorite
(Ca(OCl)2
(bleaching)
200-500 ppm Nets, boots, clothing.
Surfaces must be clean; easily inactivated by organic
debris. May be corrosive. Highly toxic for aquatic
animals.
Allow to inactivate for several days or neutralize with
sodium thiosulfate after 3 hours.
Note: Bleach is 5.25% sodium hypochlorite solution.
Iodine
(iodophors)
200 ppm for a few
seconds
Nets, boots, clothing. Surfaces must be clean;
product is easily inactivated by organic debris. Highly
toxic for aquatic animals
Benzalkonium
chloride
250 ppm Plastic surfaces - *can be toxic to fish, so use with
caution in fish areas
Alcohol
(ethanol)
70% Hand santizing
* Always consult the product label for appropriate concentrations, uses and safety precautions.
Disclaimer: The use of product names does not constitute endorsement.
Biosecurity for Aquaculture Facilities,
2009
Risk Factor: Vectors
• Wild fish
• Predators
– Birds and wildlife
• Domestic animals
– Dogs and cats
traveling between farms
• People
– Employees, Visitors
Biosecurity for Aquaculture Facilities,
2009
Prevention: Vectors (People)
Farm Visitors
– Post signs
– Maintain a visitor log
– Use foot dips/baths
for shoes
– Accompanied by
farm personnel
– Avoid animal areas
SPF seed selection
• PCR for WSSV, YHV, MBV, HPV : the PL
are automatically rejected
• LB and Vibrio Free seed
Pond preparation and biosecurity for shrimp culture

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Pond preparation and biosecurity for shrimp culture

  • 2. Diseases in shrimpPrimary • Poor Culture management Secondary • Organic matter • DO • Poor water quality • Poor climate • Staff Result • WSSV • Vibrio • White gut • White muscle • Stunted growth • Gill disease • Unknown mortalities • etc
  • 3. Bad pond preparation Leads to Low survival • Decreases pond carrying capacity • Increase disease conditions • So the survival goes down
  • 4. Bad pond preparation leads to 10 days 40 days 70 days PLANKTON: Early plankton crash/thick bloom
  • 5. Plankton Crash and Depletion of DO
  • 6. Pond bottom/ SludgeEarly Accumulation of toxic gasses
  • 7. More Vibrios and White gut incidences
  • 8. Microsporidians (EHP) • An Emerging Pathogen • Hepatopancreatic Tubule Epithelial Cells • This Farms Affected By Retarded Shrimp Growth • Exhibited High mortality associated with white faeces syndrome (WFS)
  • 9. Causes of Brownish Gill • Lumniscent / Vibrio bacteria • Low Oxygen Levels • Suspended solids • Algal Choke/plankton crash
  • 10. Viral disease more: Vectors/ carriers Improper Pond preparation and poor Biosecurity leads to WSSV
  • 11. • Prolonged Green or brown gill conditions • Deteriorated pond bottom
  • 13. Steps in Pond preparation 1 Removal of organic matter: 2 Drying: oxidation of organic matter 3 Liming: according to pH 4 Tilling: improve oxidation of nutrients and organic matter 5 Soil enrichment: adding supplements which are deficient in soils For minimum period of 30-40 days prior to every crop
  • 14. Pond bottom after crop Harvest
  • 16. Removal of organic matter • Removes anaerobic matter consist of ammonia, H2S, Methane and other anaerobic harmful substances present in sludge. • Removes certain pathogens especially Protozoans
  • 17. Sludge Vs HealthPondbottom Organic matter(Rich in organic nutrients and promotes more decaying bacteria) Waterparameter Low DO High bacteria Release of toxic gases Repeated plankton crash Shrimp Running Mortality Vibrio White gut Gills
  • 18. Drying • Effective method of eliminating undesirable species • oxidizes harmful chemicals especially sulphides • facilitates mineralization of organic matter • Decreases Carbon content
  • 20. Tilling • Improves soil quality • Speeds up the oxidation process • Release of nutrients that are locked in the soil. • Also improves the liming efficiency by mixing
  • 21. Pond Drying Vs HealthPondbottom Drying and tilling Oxidises soil Cheapest Waterparameter Eliminates many viral and bacterial pathogens of previous crop High bacteria Release of toxic gases Shrimp WSSV Vibrio Etc Premature Crop failure
  • 22. pH Check soil pH after every crop According to soil pH add liming
  • 23. Liming requirements (Mt/ha) pH CaCO3 CaOH2 7 0.65 0.33 6.5 0.9 0.45 6 1.15 0.58 5.5 1.40 0.70 5 1.65 0.83 4.5 1.90 0.95
  • 24. Liming Vs HealthPondbottom Liming Waterparameter Kills bacteria and protozoans Stabilises water pH Promotes plankton Shrimp Running Mortality Vibrio White gut Gills Moulting
  • 25. Differential of soil Organics matter overloaded has to be removed
  • 26. Differential of soil Less organics matter
  • 27. Differential of soil No organic matter
  • 28. Lined Ponds – Preparation Technique
  • 29. Compacting For Loose soils Reduces turbidity Gill chocking Improves plankton production
  • 30. SOIL ENRICHMENT DRY METHOD (ON SOIL APPLICATION): 1. Rice flour 50 kg per acre. 2. Rice Bran 20 kg per acre. 3. Probiotics 2 ltrs. Per acre. 4. Minerals 2 kg per acre. WET METHOD (WATER APPLICATION): 1. Rice flour 25 kg per acre. 2. Rice bran 10 kg per acre. 3. Jaggery 10-15 kg per acre. 4. Yeast 1 kg per acre.  Soak in water fermentation for 24-36 Hrs.
  • 31. Uses of Enrichment • Digests sludge • Promotes good bacteria • Improves nutrient cycles and bioavailability of nutrients • Balances nutrients and improves C:N ratio
  • 32. Biosecurity For Prevention of viral Diseases • To avoid pathogens • To avoid unwanted species (Mysid, fishes, molluscans etc) • To avoid vectors(Crabs, Arthropods etc) • To avoid contamination between farms and ponds
  • 33. Bio Security:  Bird Fencing  Crab Fencing  Use good meshes at water pumping (40,60,80,100)  Use PP water at enter into the farm and before enter to the farm wash your hands and legs
  • 34. Screening meshes Bird fence and Crab fence
  • 36. Reservoir/chlorination for water treatment Chemical Concentr ation Minimum withdrawal period Chlorine 20-30 ppm 3 days with aeration and sunlight Dichlorovos / Nuvon 2ppm 7 days CuSO4 (Molluscs and bivalves) 7ppm 7 days
  • 37. Biosecurity for Aquaculture Facilities, 2009 Select Disinfectants for Aquaculture Product Concentration* Uses and Precautions Virkon® Aquatic 1:100 (1%) to 1:200 (0.5%) for 1 minute Equipment, pumps, protective clothing, foot dips, bilges, bins, buckets, harvesting equipment, vehicles Calcium hypochlorite (Ca(OCl)2 (bleaching) 200-500 ppm Nets, boots, clothing. Surfaces must be clean; easily inactivated by organic debris. May be corrosive. Highly toxic for aquatic animals. Allow to inactivate for several days or neutralize with sodium thiosulfate after 3 hours. Note: Bleach is 5.25% sodium hypochlorite solution. Iodine (iodophors) 200 ppm for a few seconds Nets, boots, clothing. Surfaces must be clean; product is easily inactivated by organic debris. Highly toxic for aquatic animals Benzalkonium chloride 250 ppm Plastic surfaces - *can be toxic to fish, so use with caution in fish areas Alcohol (ethanol) 70% Hand santizing * Always consult the product label for appropriate concentrations, uses and safety precautions. Disclaimer: The use of product names does not constitute endorsement.
  • 38. Biosecurity for Aquaculture Facilities, 2009 Risk Factor: Vectors • Wild fish • Predators – Birds and wildlife • Domestic animals – Dogs and cats traveling between farms • People – Employees, Visitors
  • 39. Biosecurity for Aquaculture Facilities, 2009 Prevention: Vectors (People) Farm Visitors – Post signs – Maintain a visitor log – Use foot dips/baths for shoes – Accompanied by farm personnel – Avoid animal areas
  • 40. SPF seed selection • PCR for WSSV, YHV, MBV, HPV : the PL are automatically rejected • LB and Vibrio Free seed