In plant presentation

1. Working experience at
Ornamental Fish Breeding & Training
Center
Rambadagalla
G.Nishanthan (108552)
Department of Aquaculture & Fisheries
Faculty of Livestock, Fisheries & Nutrition
Wayamba University of Sri Lanka

2. National Aquaculture Development Authority
(NAQDA)
• Established in 1999
• Under Ministry of Fisheries and Aquatic Resources
• Main state organization responsible for development of
aquaculture industry of Sri Lanka.
• Facilitate the development of ornamental fish industry

3. Ornamental Fish Breeding And Training Center -
Rambadagalla
• Provide theoretical and practical knowledge on Fish breeding and Ornamental
plant culture
Vision
Contribute to National Social Economy Development by Empowering Ornamental
Fish and Aquatic Plant Producers of Sri Lanka
Mission
Provide a result oriented good service to Ornamental fish and Aquatic plant sector
as contribution of their development & Development of technology used in
Ornamental fish and Aquatic plant sector

4. Organizational
structure
Officer in- Charge
(OIC)
Aquaculturist
(1)
Assistant Aquaculturist
(2)
Station officer
(1)
Management assistant
(1)
Security staff
(4)
Driver
(1)
Labours
(22)

5. Main activities of Ornamental fish breeding
and training Center
• Provide training on ornamental fish breeding & farming
• Supply of quality ornamental fish brooders to the farmers
• Assist the farmers to maintain fish health
• Disease diagnosis and advisory services
• Supply of quality parent stock of ornamental aquatic plants
• Conduct adaptive research on ornamental and aquatic plant culture

6. Different Units Available
Fish Breeding
Units
Feed unit
Quarantine
Unit
Aquatic Plant
unit
Laboratory
Live bearers
Guppy
Egg Layers
Nest Builders
Fighter
Gourami
Egg Depositors
Angel
Oscar
Egg Scatters
Barb
Tetra
Koi
Gold Fish

7. Livebearer : Guppy (Poecilia reticulate)
Observed Strains :
Half Black (HB)
Red blond Tuxedo (RBT)
Golden Head (GH)
Red blond (RB)

8. R&D unit for Guppy
Available Strains
Blue Neon (BN)
Newly developed varieties
Sun Ray (SR) Copper Tail (CT)
Fire Tail (FT) Golden Tuxedo (GT) Yellow sunset (YSS)
Golden Cobra (GB) German sunset (GSS) Laser (LR)
Rambodagalla Tri colour (RTC)
Not Named Yet

9. Nest Builders : Fighter (Betta splendens)
• Available Strains :
Normal Tail
Crown Tail

10. Nest Builders : Gourami
Available Species :
3 spot Blue Opaline Golden
Rainbow Dwarf White Honey Dwarf
Pearl
Sexual Differentiation

11. Egg depositors : Angel (Pterophyllum leopoldi)
Available Strains :
Marble Platinum Blue Angel
Golden Angel Golden Strangle Zebra
Black Angel Tri colour

12. Egg depositors : Oscar (Astronotus ocellatus)
• Observed Strains :
White Oscar Black Oscar

13. Egg Scatters : Barb & Tetra
Barb Species :
Rosy barb Albino Tiger barb
Tiger barb Copper barb
Bulath hapaya
Tetra Species :
Zebra danio Buenos Aires (BA) tetra
Serpae tetra Black neon
Widow tetra

14. Barb & Tetra : Breeding Tank Setup

15. Egg scatters :Gold fish (Carassius auretus auretus)
Available Strains
Common Calico Red cap
Pearl scale Goggle eye Ranchu
Rykin Sarasa Oranda
Sexual Differentiation

16. Egg scatters : Koi (Cyprinus carpio haematopterus)
Available Strains
Black Platinum Pentium
Tancho Mirror Kohaku
Sanke Showa Shusui

17. Natural breeding
Fighter Gourami Barb
Tetra Angel Oscar
Male: Female  1:1
Gold Fish
Male: Female  2:1
Breeding Techniques of Egg Layers
Semi Artificial breeding
- For Koi
- Male: Female  2:1
- Intra-peritoneal injection of
Ovaprim (GnRH-a)  0.5mL/Kg
for female only
Artificial breeding
- For Catfish
- Male: Female  2:1
- Intra-peritoneal injection of
Ovaprim (GnRH-a)  0.5mL/Kg
for female & male

18. Feed unit
• Supply fish feed for whole farm system
Fish Feeds
Artificial Feeds
Live (Natural) Feeds
1. Moina (Moina brachiata)
2. Artemia spp.
3. Infusoria (Paramecium)
4. Bread Worm (Panagrellus redivivus)
5. Egg juice
Commercial Feeds
1. Prima 0
2. Prima 1
3. Prima 2
4. Prima Floating
Prepared Feeds
1. Grow out Feed
2. Broodstock Feed
3. Beef heart & tongue

19. Aquatic plant demonstration unit
• Over 40 plant species are available Plant Nursery
Plant Field
Potted aquatic plant
Market
Replant
Harvesting Potting
Some observed plant species
Alternanthera spp. Aponogeton rigidifolius Bacopa monnieri
Cryptocoryne spp. Echinodorus amazonicus Sagittaria sagittifolia

20. Laboratory
• Testing of water samples from outside farms
• Disease identification & treatment
• Some observed diseases :
Argulas infestation Dropsy

21. Other units

22. Achieved Learning outcomes : Soft skills
• How to work adapt in to the working environment
• Organization’s hierarchical behaviors
• Learn how to behave in an organization
• Limitations in Government organizations
• Develop personal skills
• Ready to wet my hands at any time
• Responsibility on works
• Patient
• Time management
• Flexibility

23. Achieved Learning outcomes : Hard skills
• Identification of fish species and their varieties
• Gender differentiation fish species
• Hands on experience in fish breeding & rearing techniques
• Livebearers, Nest builders, Egg depositors, Egg scatters
• Learn Natural, Semi Artificial and Artificial breeding techniques
• Fish tank preparation & water quality management
• Water quality measurement  pH, Ammonia, Temperature
• Water exchange techniques  Handling of siphoning equipment

24. Achieved Learning outcomes : Hard skills
• Learn feeding & Fish feed preparation techniques
• Artificial feeds  Broodstock, Growers, Beef heart
• Live feeds  Moina, Artemia, Infusoriya, Micro worm and Egg juice
• Technique of Guppy new variety development
• Practical knowledge on fish acclimatization and stocking

25. • Learn fish quarantine techniques
• Stress test
• Pest and predator control techniques in mud ponds
• Bird line establishment and fencing
• Fish disease identification and treatments
• Dropsy, Columnaris, white spot
• Preparation and application of disinfections and medications
• Triple mix and Methylene blue
Achieved Learning outcomes : Hard skills

26. • Identification of aquatic plant species & their cultivation
techniques
• Fish selling and marketing techniques
• Fish size separation based on prices
• Fish packing and aeration techniques
Achieved Learning outcomes : Hard skills

27. • Mud ponds are not properly maintained  Yield lost due to predators
• Use proper predator control measures
• Farm outlets are drained without any treatments to river
• Outlet must be treated before release to natural environment
• Labour dissatisfaction  Salary and work times
• Labour welfare should be developed further
Problems and Suggestions

28. • My Supervisors :
Dr. WMHK Wijenayake, Senior lecturer,
Department of Aquaculture and Fisheries
Mr. WPR Chandrarathna, OIC/ Aquaculturist,
Ornamental Fish breeding and training Centre, Rambadagalla
• Professor DS Jayakody, Head of the department and other
lecturers
• Staff and labours of Ornamental Fish breeding and training
Centre, Rambadagalla
Acknowledgement

30. Chemical / disinfectors Use Dosage
Triple mix
Prepared by mixing Formalin (1L), Malachite green (1.2g)
and Methylene blue (10g)
To treat bacterial and fungal diseases 50 ppm
Condy's (Potassium permanganate) For blanket treatment after fish harvesting 2.5ppm
H202 Used to brush walls of cement tanks and act as
a oxidizer  Remove pest eggs and larvae
80mL/L
Aquiflavin Antibacterial compound 2.5 ppm
Methylene blue Anti-bacterial agent 2.0 ppm
Neguwan Anti-parasitic agent agains Lernia and Argulas 1.0 ppm
Copper Sulfate Disinfector for siphon tubes and nets 0.3 ppm
Salt Stress testing agent 32 ppt
Salt Treating external parasite and wounds 3ppt
Diesel To kill mosquito larvae in mud ponds As needed
Chemicals and disinfectors used in Fish sections

31. Name Fish species Growth Stages Protein %
Moina (Moina brachiata) Koi, Gold fish & Catfish Nursery 50%
Artemia spp. Fry of Guppy, Gold fish, Angel,
Barb, Tetra, Fighter & Koi
Nursery &
Growing
60%
Infusoria (Paramecium) Fry of Guppy, Gold fish, Angel,
Barb, Tetra, Fighter & Koi
Nursery 50%
Bread Worm or Micro worm
(Panagrellus redivivus)
Tetra, Barb & Gourami Nursery &
Growing
40%
Egg juice Koi, Gold fish, Tetra, Barb,
Fighter & Gourami
Hatchlings 50%
Live feeds used in NAQDA

32. Parameter Value Used equipment
pH 6.5-8 Tool kit or electronic pH meter
Temperature 20-25 0C Thermometer
Dissolved
Oxygen
5 mg per liter Laboratory test
Ammonia ˂ 0.1 ppm Tool kit
Nitrate, nitric 0 Tool kit
Maintaining values of water quality parameters

33. Disease Fish varieties affected Causative agent Treatment
Columnarisa Guppy, Tetra, Barb Flexibacter columnsris, Fungi 35 ppm of triple solution, 2 ppm KMnO4 solution
Tuberculosis Guppy Mycobacterium sp, Norkadia 30 Pico gram antibiotic treatment
Dropsy Guppy, Tetra, Barb, Gold Fish, Koi Bacteria 2 ppm of KMnO4 solution
White spot Guppy, Tetra, Barb, Gold Fish, Koi Ichthyophthirius multifiliis 35 ppm of triple solution
Fin rot Angel, Tetra, Barb Nutritional deficiency 35 ppm of Triple solution, 2 ppm of KMnO4 solution
Gall bladder infection in fry Angel Bacteria 2 ppm of KMnO4 solution
Anchor worm Gold Fish, Koi Lernaea 2 ppm of KMnO4 solution
Fish louse Gold fish, Koi Argulus 2 ppm of KMnO4 solution
Tricodina Gourami Tricodina 25 ppm of Formalin solution
Fungal infection Gourami Saprolegnic 25 ppm of Formalin solution
Monegenean Gourami Dactylogyrus 25 ppm of Formalin solution
Ornamental Fish diseases and treatments

34. Guppy production Cycle
Grow out
sorting
Breeding Tank Nursery StageFry Collection &
Transfer
Grow out 1
Nursery sorting
(Select males)
Grow out 2 Market
Back up females
for broodstock

35. Production chain of Egg Layers
Breeding Tank
Nursery Tank
(15-30 days)
Mud pond
(2-3 months) Market
Backup for
broodstock
Harvesting

36. Grow out feed (1kg) preparation for live bearers
Multivitamin
+
Mineral
+
CMC
Prima 0
+
Fish Meal
Mix Well
Mix Well
Grow out feed
Can store up to 10-15
days in air tight
container

37. Preparation of Beef heart and tongue feed
Remove fat layer of beef heart &
cut into small pieces
Remove outer layer of beef
tongue & extract juicy part
Mix well
Heart: Tongue  3: 1
Blend with a blender
Put into a tray & Keep in deep
freezer

38. Broodstock feed (1kg) preparation
Multivitamin
+
Mineral
+
Vitamin E
Mix Well
Gelatin
+
40oC Water
Mix Well
Broodstock feed
Can store up to 2 -3 months in deep
freezer
Prima 0
+
Fish Meal
Mix Well
Put into tray, Make a cake
And Keep in a freezer for 24
hours
Cut into pieces and store in
deep freezer
Mix Well

39. Brine shrimp (Artemia spp.) Culture
Salt (32 g) + Water (1L)
+
Brine shrimp (1g)
Hatched & Non-hatched brine shrimp
Provide aeration
24 – 36 hours (280C)
Hatched & Non-hatched brine shrimp
Stop aeration
10 minutes
Separate hatched brine shrimp from
bottom

40. Micro worm (Panagrellus redivivus)
Mix water (250mL) + Bread to make a
paste
Mix Milk powder + Water (50mL)
Mix Well Add 2 tb. Spoon of Micro worm
culture
Use for feeding for 10-15 days
Keep closed for 4 days

41. Infusoria (Paramecium spp.)
Cut cauliflower into small pieces
Mix Milk powder + Water (50mL)
Add into container of 20L
water
Use for feeding
Cover with mosquito net &
keep for 4 days

42. Egg juice
Mix whole egg with 100mL –
250mL Water
(400C)
Use for feed
2 drops in 100mL water & feed
3-5 drops
Blend using
a blender

43. R&D unit for Guppy
• New strain development
Select Male and female
brooders form 2 different strains
Nursery
(2’ x 1’ Glass tank)
Cross and collect fry
Grow males and females
separately
Sorting
Select preferred
coloured males
Cross with
females
New Strain
Repeat until a pure strain is
obtained

44. Production chain of Nest builders (Fighter & Gourami)
Breeding Tank
Glass Tank (1’ x 0.5’)
Or
Cement Tank (For Gourami Only)
Breeding & Spawning
 Male: Female  1:1
 Egg Layers : Nest builders
 Parental care by Male
Nursery Stage (20 days)
• Fighter : Cement Tanks (5’ x 5’ )
• Gourami : In Same breeding Tank
Growing Stage
• Fighter : 5’x10’ Cement Tanks (20 days)
• Gourami : Mud ponds (60 days)
Market
Backup for
Broodstock
Harvesting

45. Guppy production Cycle
Grow out
sorting
Broodstock
Male: Female  1: 3
5’x10’ cement tank
Stocking Density: 4 fish /ft2
Nursery Stage
4’ x 4’ cement tank
Stocking density: 40fry/ft2
Period: 20 days
Fry Collection &
Transfer
Grow out 1
5’ x 5’ cement tank
SD: 20fish/ft2
Period: 30 days
Nursery sorting
(Select males)
Grow out 2
5’ x 10’ cement tank
SD: 10 fish/ft2
Period: 30 days
Market
Back up females
for broodstock

46. Nest Builders : Fighter (Betta splendens)
• Anabandidae : Labyrinth fish
• Egg Layers : Nest builders
• Territory behavior
• Observed varieties :
• Normal Tail
• Crown Tail

47. Nest Builders : Gourami
• 3 spot gourami (Trichopodus trichopterus)
• Blue Opaline Gourami (Trichogaster trichopterus)
• Golden yellow (Trichopodus trichopterus)
• Rainbow Dwarf Gourami (Trichogaster lalius)
• White Gourami (Trichogaster trichopterus)
• Honey Dwarf Gourami (Trichogaster lalius)
• Pearl Gourami (Trichopodus leerii)
Available strains

48. Production cycle of Egg depositors (Angel & Oscar)
Brooders Tank
 10’ x 5’ Cement Tank
 Female selects partner
Spawning Tank
 Male: Female  1:1
 Angel: 1.5’x1.5’x1.5’ cement tank
 Oscar: 10’ x 5 ‘ cement tank
Nursery Tanks
(30 – 60 days)
Mud pond
(2-3 months)
Market
Eggs in Plastic Basin
(14 days – 15 days)
Backup as brooders
Angel
Oscar

49. Barb & Tetra Section
• Barb & Tetra : not taxonomic terms
• Schooling fish
• Egg Scatters & No parental care
• Male : Female  1:1
• Prefer dark and acidic environment
• Observed varieties :
Barb Species
• Rosy barb (Pethia conchonius)
• Albino Tiger barb (Puntigrus tetrazona)
• Tiger barb (Puntigrus tetrazona)
• Copper barb
• Bulath hapaya (Pethiya nigrofasciatus)
Tetra Species
• Zebra danio (Danio rerio)
• Buenos Aires (BA) tetra (Hyphessobrycon anisitsi)
• Serpae tetra (Hyphessobrycon eques)
• Black neon (Hyphessobrycon herbertaxelrodi)
• Widow tetra (Gymnocorymbus ternetzi)

50. Gold fish unit (Carassius auretus auretus)
• Egg layers  Egg scatters
• No parental care
• Breeding substrate  Adhesive eggs
• Male : Female  1 : 1
• Observed varieties :
• Butterfly tail
• Fantail
• Calico
• Red cap
• Pearl scale
• Goggle eye
• Ranchu
• Lion head
• Rykin
• Sarasa
• Oranda

51. Koi unit (Cyprinus carpio haematopterus)
• Egg layers : Egg scatters
• No parental care
• Semi Artificial breeding
• Breeding substrate  Adhesive eggs
• Male : Female  1 : 1
• Observed varieties :
• Black
• Platinum
• Pentium
• Tancho
• Mirror
• Kohaku
• Sanke
• Showa
• Asagi

52. Aquatic plant demonstration unit
• 40+ plant species are available
• Some plant varieties observed :
• Alternanthera spp. (Mal Mukuna)
• Aponogeton rigidifolius (Kekatiya)
• Bacopa monnieri (Lunuwila)
• Cryptocoryne spp. (Athudian)
• Echinodorus amazonicus (Amazon Sword)
• Hemigraphis colorata
• Hydrilla verticillata
• Hydrocleys nymphoides (Water poppy)
• Hydrocotyle verticillata (Umbrella plant)
• Sagittaria sagittifolia (Arrow head plant)
Plant Nursery
Plant Field
Potted aquatic plant
Market
Replant
Harvesting Potting