SlideShare a Scribd company logo
~40%
 Hybridization
 Introgression
 Mutagenesis
 Transgenics
 etc.
Ever since Mendel—Crop genetic improvement
Genomics accelerates plant breeding
mutantWT
WT
mutant
Kaijin
WT
mutant
Kaijin
Control Treated with
0.375% NaCl
Wheat genome
IWGSC et al 2018 Science
Cirstobal Uauy
(John Innes Centre)
EMS-mutants in EnsemblPlants
Krasileva et al 2017 PNAS
Uauy et al 2017 Annual Review Genetics
Detailed info on use at
www.wheat-training.com
 Hybridization
 Introgression
 Mutagenesis
 Transgenics
 etc.
Ever since Mendel—Crop genetic improvement
Gene editing – the ultimate in precision is to
replace one of a few letters in the genome
PRR-
triggered
immunity
NLR-
triggered
immunity
effectors
bacterium
fungus
oomycete
haustorium
pathogen-associated
molecular patterns (PAMPs)
Pattern
recognition
receptors (PRRs)
NB-LRR
immune receptorsplant cell
Principles of plant-pathogen interactions
Win, J., Chaparro-Garcia, A., Belhaj, K., Saunders, D.G.O., Yoshida, K., Dong, S., Schornack, S., Zipfel, C., Robatzek, S., Hogenhout, S.A., and
Kamoun, S. Cold Spring Harbor Symposium on Quantitative Biology, 2013; Dodds, P.N., and Rathjen, J.P. Nature Reviews Genetics, 2010
targets
PRR-
triggered
immunity
NLR-
triggered
immunity
effectors
bacterium
fungus
oomycete
haustorium
pathogen-associated
molecular patterns (PAMPs)
Pattern
recognition
receptors (PRRs)
NB-LRR
immune receptorsplant cell
Principles of plant-pathogen interactions
Win, J., Chaparro-Garcia, A., Belhaj, K., Saunders, D.G.O., Yoshida, K., Dong, S., Schornack, S., Zipfel, C., Robatzek, S., Hogenhout, S.A., and
Kamoun, S. Cold Spring Harbor Symposium on Quantitative Biology, 2013; Dodds, P.N., and Rathjen, J.P. Nature Reviews Genetics, 2010
targets
R
R
S
11-9 11-20 11-24
1-1 1-14 1-24
Tomelo
Regular tomato
Tomelo—mildew fungus resistant tomato
GMO (transgenics) consist of introducing foreign
genes into plants……Genome editing can do
the opposite – removing genes from plants.
Plant genome editing is in its infancy…
Plant health emergencies in the face of a changing environment

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