The document describes the process of constructing a phylogenetic tree from mitochondrial DNA sequence data retrieved from online databases. It involves the following steps:
1) Retrieving sequence data from NCBI for humans, Neanderthals, chimpanzees, gorillas and other primates.
2) Aligning the sequences using ClustalW and viewing the alignment.
3) Using programs from the Phylip package like Seqboot, Dnadist, Neighbor and Consense to generate bootstrap trees, distance matrices and consensus trees.
4) Viewing and saving the phylogenetic trees in TreeView.
This power point is about Tree view X software and how we can made a tree by using Tree view X software, NCBI, BLAST and CLUSTAL W websites respectively. It also gives information about phylogenetic tree and its types.
It gives the evolutionary relationship among different species. This power point also explore the features of tree view X software.
The MEGA software is one of the most widely used software tools in molecular taxonomy and bioinformatics. This module describes how MEGA can be employed in a classroom setting to teach the fundamentals of molecular taxonomy.
Evolutionary tree or physlogenetic tree and it's types like rooted and unrooted labeled or unlabelled. How to construct physlogenetic tree and limitations of physlogenetic tree.
This power point is about Tree view X software and how we can made a tree by using Tree view X software, NCBI, BLAST and CLUSTAL W websites respectively. It also gives information about phylogenetic tree and its types.
It gives the evolutionary relationship among different species. This power point also explore the features of tree view X software.
The MEGA software is one of the most widely used software tools in molecular taxonomy and bioinformatics. This module describes how MEGA can be employed in a classroom setting to teach the fundamentals of molecular taxonomy.
Evolutionary tree or physlogenetic tree and it's types like rooted and unrooted labeled or unlabelled. How to construct physlogenetic tree and limitations of physlogenetic tree.
INTRODUCTION.
NCBI.
EMBL.
DDBJ.
CONCLUSION.
REFERENSE.
The National Center for Biotechnology Information (NCBI) is part of the United States National Library of Medicine (NLM), a branch of the National Institutes of Health.
The NCBI is located in Bethesda, Maryland and was founded in 1988 through legislation sponsored by Senator Claude Pepper.
The NCBI houses a series of databases relevant to biotechnology and biomedicine. Major databases include GenBank for DNA sequences and PubMed, a bibliographic database for the biomedical literature.
All these databases are available online through the Entrez search engine.
It includes the information related to a bioinformatics tool BLAST (Basic Local Alignment Search Tool), BLAST is in-silico hybridisation to find regions of similarity between biological sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance. This presentation too contains the input - output format, Blast process and its types .
lecture for doctorate students while I was working as researcher assisstance about phylogenetic science, definition,
Understand the most basic concepts of phylogeny
Understand the difference between orthology, paralogy and xenology.
Be able to compute simple phylogenetic trees
Understand what bootstrapping means in phylogeny
INTRODUCTION.
NCBI.
EMBL.
DDBJ.
CONCLUSION.
REFERENSE.
The National Center for Biotechnology Information (NCBI) is part of the United States National Library of Medicine (NLM), a branch of the National Institutes of Health.
The NCBI is located in Bethesda, Maryland and was founded in 1988 through legislation sponsored by Senator Claude Pepper.
The NCBI houses a series of databases relevant to biotechnology and biomedicine. Major databases include GenBank for DNA sequences and PubMed, a bibliographic database for the biomedical literature.
All these databases are available online through the Entrez search engine.
It includes the information related to a bioinformatics tool BLAST (Basic Local Alignment Search Tool), BLAST is in-silico hybridisation to find regions of similarity between biological sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance. This presentation too contains the input - output format, Blast process and its types .
lecture for doctorate students while I was working as researcher assisstance about phylogenetic science, definition,
Understand the most basic concepts of phylogeny
Understand the difference between orthology, paralogy and xenology.
Be able to compute simple phylogenetic trees
Understand what bootstrapping means in phylogeny
At the end of this Lesson (Part 1) the students should be able to know the following
Descriptive statistics
Saving an SPSS for Windows
Backing up your data
Retrieving your Data Files
A user's guide for a utility that creates defect wafer map images from klarf files. The program uses gnuplot to create the images. This program can be found at http://www.valaddsoft.com/Blog/products-2/
Using xbrl validation tool - XBRL Flash TutorialsAdarsh Xbrl
This tutorials teaches you how to use the XBRL Validation tool given by MCA (Ministry of Corporate Affairs).
This also gives details of saving errors, Converting XBRL instance documents to PDF.
This is not a stand alone tutorial but a part of series of XBRL Flash Tutorials by Adarsh XBRL. More such tutorials can be found at
xbrlforindiancablogspot.com
This service is all free of cost, as it is Sponsored by Madrecha & Co. (Chartered Accountants)
GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using Deplo...James Anderson
Effective Application Security in Software Delivery lifecycle using Deployment Firewall and DBOM
The modern software delivery process (or the CI/CD process) includes many tools, distributed teams, open-source code, and cloud platforms. Constant focus on speed to release software to market, along with the traditional slow and manual security checks has caused gaps in continuous security as an important piece in the software supply chain. Today organizations feel more susceptible to external and internal cyber threats due to the vast attack surface in their applications supply chain and the lack of end-to-end governance and risk management.
The software team must secure its software delivery process to avoid vulnerability and security breaches. This needs to be achieved with existing tool chains and without extensive rework of the delivery processes. This talk will present strategies and techniques for providing visibility into the true risk of the existing vulnerabilities, preventing the introduction of security issues in the software, resolving vulnerabilities in production environments quickly, and capturing the deployment bill of materials (DBOM).
Speakers:
Bob Boule
Robert Boule is a technology enthusiast with PASSION for technology and making things work along with a knack for helping others understand how things work. He comes with around 20 years of solution engineering experience in application security, software continuous delivery, and SaaS platforms. He is known for his dynamic presentations in CI/CD and application security integrated in software delivery lifecycle.
Gopinath Rebala
Gopinath Rebala is the CTO of OpsMx, where he has overall responsibility for the machine learning and data processing architectures for Secure Software Delivery. Gopi also has a strong connection with our customers, leading design and architecture for strategic implementations. Gopi is a frequent speaker and well-known leader in continuous delivery and integrating security into software delivery.
Builder.ai Founder Sachin Dev Duggal's Strategic Approach to Create an Innova...Ramesh Iyer
In today's fast-changing business world, Companies that adapt and embrace new ideas often need help to keep up with the competition. However, fostering a culture of innovation takes much work. It takes vision, leadership and willingness to take risks in the right proportion. Sachin Dev Duggal, co-founder of Builder.ai, has perfected the art of this balance, creating a company culture where creativity and growth are nurtured at each stage.
Smart TV Buyer Insights Survey 2024 by 91mobiles.pdf91mobiles
91mobiles recently conducted a Smart TV Buyer Insights Survey in which we asked over 3,000 respondents about the TV they own, aspects they look at on a new TV, and their TV buying preferences.
LF Energy Webinar: Electrical Grid Modelling and Simulation Through PowSyBl -...DanBrown980551
Do you want to learn how to model and simulate an electrical network from scratch in under an hour?
Then welcome to this PowSyBl workshop, hosted by Rte, the French Transmission System Operator (TSO)!
During the webinar, you will discover the PowSyBl ecosystem as well as handle and study an electrical network through an interactive Python notebook.
PowSyBl is an open source project hosted by LF Energy, which offers a comprehensive set of features for electrical grid modelling and simulation. Among other advanced features, PowSyBl provides:
- A fully editable and extendable library for grid component modelling;
- Visualization tools to display your network;
- Grid simulation tools, such as power flows, security analyses (with or without remedial actions) and sensitivity analyses;
The framework is mostly written in Java, with a Python binding so that Python developers can access PowSyBl functionalities as well.
What you will learn during the webinar:
- For beginners: discover PowSyBl's functionalities through a quick general presentation and the notebook, without needing any expert coding skills;
- For advanced developers: master the skills to efficiently apply PowSyBl functionalities to your real-world scenarios.
Elevating Tactical DDD Patterns Through Object CalisthenicsDorra BARTAGUIZ
After immersing yourself in the blue book and its red counterpart, attending DDD-focused conferences, and applying tactical patterns, you're left with a crucial question: How do I ensure my design is effective? Tactical patterns within Domain-Driven Design (DDD) serve as guiding principles for creating clear and manageable domain models. However, achieving success with these patterns requires additional guidance. Interestingly, we've observed that a set of constraints initially designed for training purposes remarkably aligns with effective pattern implementation, offering a more ‘mechanical’ approach. Let's explore together how Object Calisthenics can elevate the design of your tactical DDD patterns, offering concrete help for those venturing into DDD for the first time!
Essentials of Automations: Optimizing FME Workflows with ParametersSafe Software
Are you looking to streamline your workflows and boost your projects’ efficiency? Do you find yourself searching for ways to add flexibility and control over your FME workflows? If so, you’re in the right place.
Join us for an insightful dive into the world of FME parameters, a critical element in optimizing workflow efficiency. This webinar marks the beginning of our three-part “Essentials of Automation” series. This first webinar is designed to equip you with the knowledge and skills to utilize parameters effectively: enhancing the flexibility, maintainability, and user control of your FME projects.
Here’s what you’ll gain:
- Essentials of FME Parameters: Understand the pivotal role of parameters, including Reader/Writer, Transformer, User, and FME Flow categories. Discover how they are the key to unlocking automation and optimization within your workflows.
- Practical Applications in FME Form: Delve into key user parameter types including choice, connections, and file URLs. Allow users to control how a workflow runs, making your workflows more reusable. Learn to import values and deliver the best user experience for your workflows while enhancing accuracy.
- Optimization Strategies in FME Flow: Explore the creation and strategic deployment of parameters in FME Flow, including the use of deployment and geometry parameters, to maximize workflow efficiency.
- Pro Tips for Success: Gain insights on parameterizing connections and leveraging new features like Conditional Visibility for clarity and simplicity.
We’ll wrap up with a glimpse into future webinars, followed by a Q&A session to address your specific questions surrounding this topic.
Don’t miss this opportunity to elevate your FME expertise and drive your projects to new heights of efficiency.
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Connector Corner: Automate dynamic content and events by pushing a buttonDianaGray10
Here is something new! In our next Connector Corner webinar, we will demonstrate how you can use a single workflow to:
Create a campaign using Mailchimp with merge tags/fields
Send an interactive Slack channel message (using buttons)
Have the message received by managers and peers along with a test email for review
But there’s more:
In a second workflow supporting the same use case, you’ll see:
Your campaign sent to target colleagues for approval
If the “Approve” button is clicked, a Jira/Zendesk ticket is created for the marketing design team
But—if the “Reject” button is pushed, colleagues will be alerted via Slack message
Join us to learn more about this new, human-in-the-loop capability, brought to you by Integration Service connectors.
And...
Speakers:
Akshay Agnihotri, Product Manager
Charlie Greenberg, Host
JMeter webinar - integration with InfluxDB and GrafanaRTTS
Watch this recorded webinar about real-time monitoring of application performance. See how to integrate Apache JMeter, the open-source leader in performance testing, with InfluxDB, the open-source time-series database, and Grafana, the open-source analytics and visualization application.
In this webinar, we will review the benefits of leveraging InfluxDB and Grafana when executing load tests and demonstrate how these tools are used to visualize performance metrics.
Length: 30 minutes
Session Overview
-------------------------------------------
During this webinar, we will cover the following topics while demonstrating the integrations of JMeter, InfluxDB and Grafana:
- What out-of-the-box solutions are available for real-time monitoring JMeter tests?
- What are the benefits of integrating InfluxDB and Grafana into the load testing stack?
- Which features are provided by Grafana?
- Demonstration of InfluxDB and Grafana using a practice web application
To view the webinar recording, go to:
https://www.rttsweb.com/jmeter-integration-webinar
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UI automation Introduction,
UI automation Sample
Desktop automation flow
Pradeep Chinnala, Senior Consultant Automation Developer @WonderBotz and UiPath MVP
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
Generating a custom Ruby SDK for your web service or Rails API using Smithyg2nightmarescribd
Have you ever wanted a Ruby client API to communicate with your web service? Smithy is a protocol-agnostic language for defining services and SDKs. Smithy Ruby is an implementation of Smithy that generates a Ruby SDK using a Smithy model. In this talk, we will explore Smithy and Smithy Ruby to learn how to generate custom feature-rich SDKs that can communicate with any web service, such as a Rails JSON API.
6. Find the most relevant nucleotide sequence from database. I prefer to ch oose mitochondrial D-loop hypervariable region I to further multiple alignment and phylogenetic tree analysis.
7. After clicking the sequence, the web page displays as shown in the figure. You can select among various types of format such as FASTA, Genbank, and XML. I prefer to use FASTA format and save to notepad program.
8. Open the NCBI web page again. Type “mitochondrial D-loop hypervariable region I human” in the search box.
10. Try to find more relevant sequence, I go to next page as shown in the figure, and choose sequence in the red oval mark.
11. After clicking the sequence, the web page displays as shown in the figure. You can select among various types of format such as FASTA, Genbank, and XML. I prefer to use FASTA format and save to notepad program.
14. After clicking the sequence, the web page displays as shown in the figure. You can select among various types of format such as FASTA, Genbank, and XML. I prefer to use FASTA format and save to notepad program.
17. After clicking the sequence, the web page displays as shown in the figure. You can select among various types of format such as FASTA, Genbank, and XML. I prefer to use FASTA format and save to notepad program.
20. After clicking the sequence, the web page displays as shown in the figure. You can select among various types of format such as FASTA, Genbank, and XML. I prefer to use FASTA format and save to notepad program.
23. After clicking the sequence, the web page displays as shown in the figure. You can select among various types of format such as FASTA, Genbank, and XML. I prefer to use FASTA format and save to notepad program.
26. After clicking the sequence, the web page displays as shown in the figure. You can select among various types of format such as FASTA, Genbank, and XML. I prefer to use FASTA format and save to notepad program.
27. These are whole FASTA formats from NCBI database including human, Neanderthal, chimpanzee, gorilla, orangutan, siamang, and bonobo.
32. Upload the file(al.txt) from computer, and then click the run butoon to let the clustalw execute
33. This figure represents the result from clustalW, and you can save result to the computer by right clicking the file and selecting “save target as”. It automatically downloads to your PC or laptop . Right-click
34. This figure presents result files from ClustalW. alignment file guide tree file output file
41. After Clicking win32, the popup window display as in the figure. Then select save to disk to start downloading the program.
42. After downloading completely, treev32 file appears on the desktop. This figure shows after opening file with the winrar program. Then, double-click the setup file to install the program.
43. The installshield wizard appears on desktop automaically after doule-clicking the setup file.Then, click the next button untill fisnishing the installation process.
50. After click the first website, the home page shows as in the picture. Then click the area that is in red oval shape.
51. The Popup window shows on screen after clicking the “Bioedit.zip”. Select “save to disk” to download to the computer.
52. Use the winrar program to open the file and double-click the setup file to start installing the program . After downloading completely, you get the bioedit file.
53. The installshield wizard show on desktop automaically after doule-clicking the setup file.Then, click the next button untill fisnishing the installation process.
54. After completing install program, press start tab, click all programs and choose the the bioedit to lauach the program.
55. After clicking the biodeit, the program’s graphic user interface shows as in the figure. Open the previous aligment file from clustalW.
56. This figure shows the alignment result . Go to file menu and select save as.
57. Save the file to computer My file is mDNA.phy file. The mDNA file appears on the desktop.
58. Creating tree by Phylip Open the website “google.co.th” and type “phylip” in the search box.
80. Double click the consense program. This program requires intree created by the neighbor program. Consense tree
81. Key letter “ y” and press enter to start the program. After finishing the concense program, you get the outfile and outtre files .
82. Open the outtree file created by the consense program with the treeview software.
83. This figure shows all kinds of phylogenetic tree. radial tree cladogram rectangular cladogram phylogram
84. You can save entire phylogenetic trees to the computer by going to fie menu and choosing save as graphic. After saving completely, all files appear on the desktop.