Polymerase chain reaction (PCR) is a technique used to amplify specific regions of DNA. It allows scientists to make millions to billions of copies of the target DNA sequence. Real-time quantitative PCR (qPCR) allows quantification of the amount of target DNA or RNA present. In situ hybridization is a technique that uses labeled nucleic acid probes to localize specific DNA or RNA sequences within cells in preserved tissue samples.

2. Polymerase Chain Reaction (PCR)

14. Polymerase Chain Reaction

16. Multiplex PCR

22. In order to use PCR, one must know the sequences which flank both ends of a given region of interest in DNA . One need not know the DNA sequence in-between.

30. Real-Time qRT-PCR

45. The principle of Taqman qPCR

48. In situ hybridisation