This chapter discusses the discovery of DNA and its role as the genetic material. It covers Griffith's experiments showing bacterial transformation, the Hershey-Chase experiment demonstrating that DNA carries genetic information in viruses, and Chargaff's rules which helped Watson and Crick determine DNA's double-helix structure. The chapter also addresses DNA replication, including the roles of enzymes and telomeres, and how replication occurs differently in prokaryotes and eukaryotes.