DNA is the genetic material contained in chromosomes. Key experiments including Griffith's transformation experiment, Avery's transformation experiment, and the Hershey-Chase experiment provided evidence that DNA, not protein or RNA, is the genetic material and is passed from parent to offspring during reproduction. DNA has a double helix structure with nucleotides containing nitrogenous bases and a phosphate sugar backbone. DNA is organized into chromosomes that package the DNA inside cells.
Genetic material early experiments structure of DNA.pdfMohamed Alashram
The key early experiments that identified DNA as the genetic material included Griffith's 1928 experiment demonstrating bacterial transformation, Avery's 1944 experiment showing that DNA was the transforming agent, and the 1953 Hershey-Chase experiment which found that DNA rather than protein entered bacteria during viral infection. Further experiments in the 1950s by Gierer and Schramm and others showed that RNA could act as the genetic material of some viruses. Finally, Watson and Crick's 1953 proposal of the double helix structure of DNA, based on Chargaff's rules and X-ray crystallography data, provided the molecular mechanism for DNA's role in heredity and replication.
The document provides a historical overview of key discoveries related to DNA as the genetic material:
1) In the early 1900s, chromosomes were shown to carry hereditary information. By the 1940s-1950s, experiments by Avery, Griffith, Hershey and Chase provided evidence that DNA - not protein - was the genetic material.
2) Watson and Crick proposed the double helix structure of DNA in 1953 based on Chargaff's rules and Franklin's X-ray crystallography photos. Their model explained how DNA replicates and hereditary information is passed from parents to offspring.
3) Subsequent work in the 1960s by Nirenberg, Matthaei and others cracked the
This document provides information on DNA structure and replication. It describes that DNA is made of two polynucleotide chains held together by hydrogen bonds between complementary nucleotide bases. Replication of DNA is semiconservative, meaning each new DNA molecule contains one original and one newly synthesized strand. Experiments by Meselson and Stahl using isotopes of nitrogen demonstrated that replication results in daughter DNA molecules with one original and one new strand, supporting the semiconservative model proposed by Watson and Crick.
genetic material in organization, Central dogma,transcription in prokaryotes ...Patelrushi11
Historical background of molecular genetics, genetics material in organisams- Experiments, Nucleic acid as genetic material, central dogma, transcription in prokaryotes eukaryotes, genetic codegenetic code and its characteristics, silent feature of genetic codon,wobbal hypothesis
The presentation includes about the basic knowledge of Deoxyribonucleic Acid or DNA. It involves the definition, structure, occurence, quantity, chemical composition, stability, variety, types, molecular weight, complementary of base pairs, absorbance, viscosity, ionic interactions, alternative forms and functions of DNA.
The document provides a history of discoveries related to DNA and genetics. It describes experiments in the 1940s-1950s that proved DNA is the genetic material responsible for inheritance. It details discoveries such as DNA's double helix structure and base pairing rules. The document also summarizes the mapping of the human genome and benefits of understanding the genome sequence.
Genetic material early experiments structure of DNA.pdfMohamed Alashram
The key early experiments that identified DNA as the genetic material included Griffith's 1928 experiment demonstrating bacterial transformation, Avery's 1944 experiment showing that DNA was the transforming agent, and the 1953 Hershey-Chase experiment which found that DNA rather than protein entered bacteria during viral infection. Further experiments in the 1950s by Gierer and Schramm and others showed that RNA could act as the genetic material of some viruses. Finally, Watson and Crick's 1953 proposal of the double helix structure of DNA, based on Chargaff's rules and X-ray crystallography data, provided the molecular mechanism for DNA's role in heredity and replication.
The document provides a historical overview of key discoveries related to DNA as the genetic material:
1) In the early 1900s, chromosomes were shown to carry hereditary information. By the 1940s-1950s, experiments by Avery, Griffith, Hershey and Chase provided evidence that DNA - not protein - was the genetic material.
2) Watson and Crick proposed the double helix structure of DNA in 1953 based on Chargaff's rules and Franklin's X-ray crystallography photos. Their model explained how DNA replicates and hereditary information is passed from parents to offspring.
3) Subsequent work in the 1960s by Nirenberg, Matthaei and others cracked the
This document provides information on DNA structure and replication. It describes that DNA is made of two polynucleotide chains held together by hydrogen bonds between complementary nucleotide bases. Replication of DNA is semiconservative, meaning each new DNA molecule contains one original and one newly synthesized strand. Experiments by Meselson and Stahl using isotopes of nitrogen demonstrated that replication results in daughter DNA molecules with one original and one new strand, supporting the semiconservative model proposed by Watson and Crick.
genetic material in organization, Central dogma,transcription in prokaryotes ...Patelrushi11
Historical background of molecular genetics, genetics material in organisams- Experiments, Nucleic acid as genetic material, central dogma, transcription in prokaryotes eukaryotes, genetic codegenetic code and its characteristics, silent feature of genetic codon,wobbal hypothesis
The presentation includes about the basic knowledge of Deoxyribonucleic Acid or DNA. It involves the definition, structure, occurence, quantity, chemical composition, stability, variety, types, molecular weight, complementary of base pairs, absorbance, viscosity, ionic interactions, alternative forms and functions of DNA.
The document provides a history of discoveries related to DNA and genetics. It describes experiments in the 1940s-1950s that proved DNA is the genetic material responsible for inheritance. It details discoveries such as DNA's double helix structure and base pairing rules. The document also summarizes the mapping of the human genome and benefits of understanding the genome sequence.
1. Frederick Griffith discovered in 1928 that a "rough" non-pathogenic strain of pneumonia bacteria could be transformed into a "smooth" pathogenic strain through exposure to heat-killed pathogenic bacteria.
2. Hershey and Chase provided evidence in 1952 that DNA, not protein, was the genetic material through experiments using radioactive labeling of bacteriophages.
3. Watson and Crick deduced the double-helix structure of DNA in 1953 based on Chargaff's rules of DNA composition and Rosalind Franklin's X-ray crystallography photos of DNA.
research done to prove DNA a genetic materialPartha Sarathi
1. Genetic inheritance refers to the transmission of traits from parents to offspring through genetic material. DNA was identified as the genetic material based on its ability to stably replicate and mutate over generations.
2. Experiments in the early 20th century identified DNA as the substance within chromosomes that determines inheritance. Key experiments included Avery, MacLeod and McCarty demonstrating transformation is caused by DNA.
3. The structure of DNA was elucidated in 1953 when Watson and Crick proposed the double helix model based on X-ray crystallography data from Franklin and Chargaff's rules regarding nucleotide base ratios. This established DNA as the molecule of heredity.
1) The document provides an overview of DNA structure and function. It describes DNA as the genetic material that carries hereditary information from one generation to the next in the form of genes.
2) The key experiments that proved DNA is the genetic material are described, including Griffith's transformation experiment, Avery's work showing the transforming principle is DNA, and Hershey and Chase's experiment using radioactive labeling of DNA and proteins in bacteriophages.
3) Watson and Crick are credited with discovering the double helix structure of DNA in 1953 based on Chargaff's rules of base pairing and X-ray crystallography data. Their model explained DNA's ability to self-replicate semiconserv
DNA : a genetic material, replication damage and repairAnilkumar C
The document provides information on DNA, including its identification as the genetic material, its structure and replication. Some key points:
- Experiments in the 1920s-1950s identified DNA as the genetic material, including Griffith's work on bacterial transformation and the experiments of Avery, MacLeod and McCarty and Hershey and Chase.
- DNA is made up of nucleotides containing phosphate groups, sugars and nitrogenous bases. Watson and Crick discovered its double helix structure in 1953, with two anti-parallel strands held together by hydrogen bonds between complementary bases.
- DNA replication is semi-conservative and involves unwinding of the DNA strands, synthesis of new complementary strands, and production of identical double
DNA is made of two linked strands that wind around each other to resemble a twisted ladder — a shape known as a double helix. Each strand has a backbone made of alternating sugar (deoxyribose) and phosphate groups. Attached to each sugar is one of four bases: adenine (A), cytosine (C), guanine (G) or thymine (T).
CH- 6 MOLECULAR BASIS OF INHERITANCE (1).pdfSunitaKumar24
DNA is made up of two polynucleotide chains that are coiled together in a double helix structure. Each chain contains deoxyribonucleotides joined by phosphodiester bonds. The nucleotides consist of a pentose sugar, phosphate group, and one of four nitrogenous bases - adenine, guanine, cytosine, or thymine. The bases on each chain pair up through hydrogen bonds to form base pairs between adenine and thymine or cytosine and guanine. DNA replicates semiconservatively, with each new DNA molecule containing one original and one newly synthesized strand.
Basic concepts & scope of recombinant DNA technologyRavi Kant Agrawal
Recombinant DNA technology involves combining DNA molecules from different sources and introducing them into host cells. Key developments include the discovery that DNA carries genetic information (Avery, 1944), determining DNA's structure (Watson and Crick, 1953), developing techniques to cut and join DNA (restriction enzymes and ligase, 1970s), and creating the first recombinant DNA molecules by combining bacterial plasmid and phage DNA (Cohen and Boyer, 1973). These advances laid the foundation for genetic engineering.
Fredrick Griffith discovered that heat-killed pneumonia bacteria could transform harmless bacteria into deadly disease-causing bacteria when mixed together and injected into mice. This led researchers like Oswald Avery to determine that DNA was the molecule responsible for transformation. Further experiments by Hershey and Chase showed that the genetic material of viruses that infect bacteria is DNA. Together, these findings established DNA as the genetic material that gets passed from parents to offspring and controls inheritance.
The document summarizes the central dogma of biology and the discovery of DNA as the genetic material. It describes key experiments that showed DNA replicates in a semiconservative manner, with each parental strand serving as a template for a new complementary daughter strand. The process of DNA replication requires several enzymes including DNA polymerase, helicase, ligase and primase to unwind, copy and join new DNA strands.
The document discusses the hierarchy of knowledge in biochemistry, molecular biology, and biotechnology. It begins by explaining how biochemistry initially focused on proteins and enzymes, while molecular biology focused on nucleic acids and the structure and function of genes. Biotechnology emerged due to advances in molecular biology and recombinant DNA techniques. The key areas of each discipline are defined, including how molecular biology studies how organisms are made from simple molecules at the cellular level.
This document provides an overview of molecular genetics and biotechnology. It discusses the structure of DNA and the DNA double helix model. It explains DNA replication, which is essential for cells to copy genetic material before cell division. Gene expression through transcription and translation is summarized, including how DNA is copied into mRNA and then translated into proteins. The sequencing of the human genome is mentioned, along with applications like disease diagnosis. Genetic engineering techniques like cloning and genetically modified organisms are defined. Finally, biotechnology applications in agriculture, medicine, and industry are briefly described.
This document provides information on genetics and DNA. It discusses that DNA is found coiled in chromatin in the nucleus of cells. Chromosomes contain duplicated DNA and genes. DNA is made of nucleotides containing nitrogenous bases and sugars. Watson and Crick discovered that DNA has a double helix structure with adenine bonding with thymine and guanine bonding with cytosine. DNA replicates semi-conservatively before cell division. Genes can be cloned using recombinant DNA technology or PCR to produce proteins like insulin for medical use.
The document provides an overview of DNA structure and function. It discusses early experiments that established DNA as the genetic material, including Griffith's experiments showing transformation in bacteria and Avery, Macleod and McCarty's experiments proving that DNA is the transforming principle. It describes Chargaff's rules, Watson and Crick's proposal of the double helix model based on X-ray diffraction data, and semiconservative DNA replication demonstrated by Meselson and Stahl's experiment using nitrogen isotopes.
This document provides an overview of molecular genetics and biotechnology. It discusses the structure of DNA and the DNA double helix model. It explains DNA replication, which is essential for cells to copy genetic material before cell division. Gene expression through transcription and translation is summarized, including how DNA is copied into mRNA and then translated into proteins. The sequencing of the human genome is mentioned, along with applications like disease diagnosis. Genetic engineering techniques like isolating, inserting, and recombining genes are outlined. Uses of biotechnology in agriculture, farming, and medicine are also reviewed.
This document discusses genetic material and its organization into chromosomes. It begins by outlining the requirements for something to be considered a genetic material, including that it must carry information, self-replicate, allow for changes in information, and govern phenotype expression. The identification of DNA as the genetic material is then summarized, from Griffith's discovery of transformation in bacteria to the conclusive experiments of Avery, MacLeod, and McCarty. The structure of DNA is covered briefly, including Watson and Crick's double helix model. DNA replication and repair mechanisms such as base excision repair, nucleotide excision repair, and mismatch repair are then summarized in 1-2 sentences each.
DNA replication is semiconservative and bidirectional. It involves unwinding the parental DNA strands at an origin of replication followed by synthesis of new complementary strands. Each parental strand serves as a template for a new daughter strand. DNA polymerase adds nucleotides to the 3' end of the growing strand based on complementary base pairing. The leading strand is synthesized continuously while the lagging strand is synthesized discontinuously in short segments called Okazaki fragments. RNA primers are required for initiation and DNA polymerase proofreads and corrects errors to ensure high fidelity. Telomeres and the telomerase enzyme allow complete replication of linear chromosomes.
The document discusses DNA replication. It describes early experiments that showed DNA carries genetic information, such as the Avery-MacLeod-McCarty experiment. It also describes Chargaff's rules about DNA base composition and the Watson and Crick model of the DNA double helix structure. The process of DNA replication is then explained, including semi-conservative replication, the role of enzymes like DNA polymerase and helicase, and leading and lagging strand synthesis.
This document provides information on genetics and DNA. It discusses that DNA is found coiled in chromatin in the nucleus of cells. During cell division, chromatin coils tightly to form chromosomes which are duplicated so each new cell contains a full set. DNA is made of nucleotides containing a sugar, phosphate, and one of four nitrogenous bases. The bases bond with each other to form the sides of the DNA double helix structure. Experiments by Griffith, Hershey and Chase provided evidence that DNA is the genetic material. Watson and Crick used evidence from Franklin and others to develop the first model of the DNA double helix structure. DNA holds the instructions for development and reproduction and can replicate itself through semiconservative replication.
How to Setup Warehouse & Location in Odoo 17 InventoryCeline George
In this slide, we'll explore how to set up warehouses and locations in Odoo 17 Inventory. This will help us manage our stock effectively, track inventory levels, and streamline warehouse operations.
1. Frederick Griffith discovered in 1928 that a "rough" non-pathogenic strain of pneumonia bacteria could be transformed into a "smooth" pathogenic strain through exposure to heat-killed pathogenic bacteria.
2. Hershey and Chase provided evidence in 1952 that DNA, not protein, was the genetic material through experiments using radioactive labeling of bacteriophages.
3. Watson and Crick deduced the double-helix structure of DNA in 1953 based on Chargaff's rules of DNA composition and Rosalind Franklin's X-ray crystallography photos of DNA.
research done to prove DNA a genetic materialPartha Sarathi
1. Genetic inheritance refers to the transmission of traits from parents to offspring through genetic material. DNA was identified as the genetic material based on its ability to stably replicate and mutate over generations.
2. Experiments in the early 20th century identified DNA as the substance within chromosomes that determines inheritance. Key experiments included Avery, MacLeod and McCarty demonstrating transformation is caused by DNA.
3. The structure of DNA was elucidated in 1953 when Watson and Crick proposed the double helix model based on X-ray crystallography data from Franklin and Chargaff's rules regarding nucleotide base ratios. This established DNA as the molecule of heredity.
1) The document provides an overview of DNA structure and function. It describes DNA as the genetic material that carries hereditary information from one generation to the next in the form of genes.
2) The key experiments that proved DNA is the genetic material are described, including Griffith's transformation experiment, Avery's work showing the transforming principle is DNA, and Hershey and Chase's experiment using radioactive labeling of DNA and proteins in bacteriophages.
3) Watson and Crick are credited with discovering the double helix structure of DNA in 1953 based on Chargaff's rules of base pairing and X-ray crystallography data. Their model explained DNA's ability to self-replicate semiconserv
DNA : a genetic material, replication damage and repairAnilkumar C
The document provides information on DNA, including its identification as the genetic material, its structure and replication. Some key points:
- Experiments in the 1920s-1950s identified DNA as the genetic material, including Griffith's work on bacterial transformation and the experiments of Avery, MacLeod and McCarty and Hershey and Chase.
- DNA is made up of nucleotides containing phosphate groups, sugars and nitrogenous bases. Watson and Crick discovered its double helix structure in 1953, with two anti-parallel strands held together by hydrogen bonds between complementary bases.
- DNA replication is semi-conservative and involves unwinding of the DNA strands, synthesis of new complementary strands, and production of identical double
DNA is made of two linked strands that wind around each other to resemble a twisted ladder — a shape known as a double helix. Each strand has a backbone made of alternating sugar (deoxyribose) and phosphate groups. Attached to each sugar is one of four bases: adenine (A), cytosine (C), guanine (G) or thymine (T).
CH- 6 MOLECULAR BASIS OF INHERITANCE (1).pdfSunitaKumar24
DNA is made up of two polynucleotide chains that are coiled together in a double helix structure. Each chain contains deoxyribonucleotides joined by phosphodiester bonds. The nucleotides consist of a pentose sugar, phosphate group, and one of four nitrogenous bases - adenine, guanine, cytosine, or thymine. The bases on each chain pair up through hydrogen bonds to form base pairs between adenine and thymine or cytosine and guanine. DNA replicates semiconservatively, with each new DNA molecule containing one original and one newly synthesized strand.
Basic concepts & scope of recombinant DNA technologyRavi Kant Agrawal
Recombinant DNA technology involves combining DNA molecules from different sources and introducing them into host cells. Key developments include the discovery that DNA carries genetic information (Avery, 1944), determining DNA's structure (Watson and Crick, 1953), developing techniques to cut and join DNA (restriction enzymes and ligase, 1970s), and creating the first recombinant DNA molecules by combining bacterial plasmid and phage DNA (Cohen and Boyer, 1973). These advances laid the foundation for genetic engineering.
Fredrick Griffith discovered that heat-killed pneumonia bacteria could transform harmless bacteria into deadly disease-causing bacteria when mixed together and injected into mice. This led researchers like Oswald Avery to determine that DNA was the molecule responsible for transformation. Further experiments by Hershey and Chase showed that the genetic material of viruses that infect bacteria is DNA. Together, these findings established DNA as the genetic material that gets passed from parents to offspring and controls inheritance.
The document summarizes the central dogma of biology and the discovery of DNA as the genetic material. It describes key experiments that showed DNA replicates in a semiconservative manner, with each parental strand serving as a template for a new complementary daughter strand. The process of DNA replication requires several enzymes including DNA polymerase, helicase, ligase and primase to unwind, copy and join new DNA strands.
The document discusses the hierarchy of knowledge in biochemistry, molecular biology, and biotechnology. It begins by explaining how biochemistry initially focused on proteins and enzymes, while molecular biology focused on nucleic acids and the structure and function of genes. Biotechnology emerged due to advances in molecular biology and recombinant DNA techniques. The key areas of each discipline are defined, including how molecular biology studies how organisms are made from simple molecules at the cellular level.
This document provides an overview of molecular genetics and biotechnology. It discusses the structure of DNA and the DNA double helix model. It explains DNA replication, which is essential for cells to copy genetic material before cell division. Gene expression through transcription and translation is summarized, including how DNA is copied into mRNA and then translated into proteins. The sequencing of the human genome is mentioned, along with applications like disease diagnosis. Genetic engineering techniques like cloning and genetically modified organisms are defined. Finally, biotechnology applications in agriculture, medicine, and industry are briefly described.
This document provides information on genetics and DNA. It discusses that DNA is found coiled in chromatin in the nucleus of cells. Chromosomes contain duplicated DNA and genes. DNA is made of nucleotides containing nitrogenous bases and sugars. Watson and Crick discovered that DNA has a double helix structure with adenine bonding with thymine and guanine bonding with cytosine. DNA replicates semi-conservatively before cell division. Genes can be cloned using recombinant DNA technology or PCR to produce proteins like insulin for medical use.
The document provides an overview of DNA structure and function. It discusses early experiments that established DNA as the genetic material, including Griffith's experiments showing transformation in bacteria and Avery, Macleod and McCarty's experiments proving that DNA is the transforming principle. It describes Chargaff's rules, Watson and Crick's proposal of the double helix model based on X-ray diffraction data, and semiconservative DNA replication demonstrated by Meselson and Stahl's experiment using nitrogen isotopes.
This document provides an overview of molecular genetics and biotechnology. It discusses the structure of DNA and the DNA double helix model. It explains DNA replication, which is essential for cells to copy genetic material before cell division. Gene expression through transcription and translation is summarized, including how DNA is copied into mRNA and then translated into proteins. The sequencing of the human genome is mentioned, along with applications like disease diagnosis. Genetic engineering techniques like isolating, inserting, and recombining genes are outlined. Uses of biotechnology in agriculture, farming, and medicine are also reviewed.
This document discusses genetic material and its organization into chromosomes. It begins by outlining the requirements for something to be considered a genetic material, including that it must carry information, self-replicate, allow for changes in information, and govern phenotype expression. The identification of DNA as the genetic material is then summarized, from Griffith's discovery of transformation in bacteria to the conclusive experiments of Avery, MacLeod, and McCarty. The structure of DNA is covered briefly, including Watson and Crick's double helix model. DNA replication and repair mechanisms such as base excision repair, nucleotide excision repair, and mismatch repair are then summarized in 1-2 sentences each.
DNA replication is semiconservative and bidirectional. It involves unwinding the parental DNA strands at an origin of replication followed by synthesis of new complementary strands. Each parental strand serves as a template for a new daughter strand. DNA polymerase adds nucleotides to the 3' end of the growing strand based on complementary base pairing. The leading strand is synthesized continuously while the lagging strand is synthesized discontinuously in short segments called Okazaki fragments. RNA primers are required for initiation and DNA polymerase proofreads and corrects errors to ensure high fidelity. Telomeres and the telomerase enzyme allow complete replication of linear chromosomes.
The document discusses DNA replication. It describes early experiments that showed DNA carries genetic information, such as the Avery-MacLeod-McCarty experiment. It also describes Chargaff's rules about DNA base composition and the Watson and Crick model of the DNA double helix structure. The process of DNA replication is then explained, including semi-conservative replication, the role of enzymes like DNA polymerase and helicase, and leading and lagging strand synthesis.
This document provides information on genetics and DNA. It discusses that DNA is found coiled in chromatin in the nucleus of cells. During cell division, chromatin coils tightly to form chromosomes which are duplicated so each new cell contains a full set. DNA is made of nucleotides containing a sugar, phosphate, and one of four nitrogenous bases. The bases bond with each other to form the sides of the DNA double helix structure. Experiments by Griffith, Hershey and Chase provided evidence that DNA is the genetic material. Watson and Crick used evidence from Franklin and others to develop the first model of the DNA double helix structure. DNA holds the instructions for development and reproduction and can replicate itself through semiconservative replication.
How to Setup Warehouse & Location in Odoo 17 InventoryCeline George
In this slide, we'll explore how to set up warehouses and locations in Odoo 17 Inventory. This will help us manage our stock effectively, track inventory levels, and streamline warehouse operations.
Walmart Business+ and Spark Good for Nonprofits.pdfTechSoup
"Learn about all the ways Walmart supports nonprofit organizations.
You will hear from Liz Willett, the Head of Nonprofits, and hear about what Walmart is doing to help nonprofits, including Walmart Business and Spark Good. Walmart Business+ is a new offer for nonprofits that offers discounts and also streamlines nonprofits order and expense tracking, saving time and money.
The webinar may also give some examples on how nonprofits can best leverage Walmart Business+.
The event will cover the following::
Walmart Business + (https://business.walmart.com/plus) is a new shopping experience for nonprofits, schools, and local business customers that connects an exclusive online shopping experience to stores. Benefits include free delivery and shipping, a 'Spend Analytics” feature, special discounts, deals and tax-exempt shopping.
Special TechSoup offer for a free 180 days membership, and up to $150 in discounts on eligible orders.
Spark Good (walmart.com/sparkgood) is a charitable platform that enables nonprofits to receive donations directly from customers and associates.
Answers about how you can do more with Walmart!"
Strategies for Effective Upskilling is a presentation by Chinwendu Peace in a Your Skill Boost Masterclass organisation by the Excellence Foundation for South Sudan on 08th and 09th June 2024 from 1 PM to 3 PM on each day.
Exploiting Artificial Intelligence for Empowering Researchers and Faculty, In...Dr. Vinod Kumar Kanvaria
Exploiting Artificial Intelligence for Empowering Researchers and Faculty,
International FDP on Fundamentals of Research in Social Sciences
at Integral University, Lucknow, 06.06.2024
By Dr. Vinod Kumar Kanvaria
ISO/IEC 27001, ISO/IEC 42001, and GDPR: Best Practices for Implementation and...PECB
Denis is a dynamic and results-driven Chief Information Officer (CIO) with a distinguished career spanning information systems analysis and technical project management. With a proven track record of spearheading the design and delivery of cutting-edge Information Management solutions, he has consistently elevated business operations, streamlined reporting functions, and maximized process efficiency.
Certified as an ISO/IEC 27001: Information Security Management Systems (ISMS) Lead Implementer, Data Protection Officer, and Cyber Risks Analyst, Denis brings a heightened focus on data security, privacy, and cyber resilience to every endeavor.
His expertise extends across a diverse spectrum of reporting, database, and web development applications, underpinned by an exceptional grasp of data storage and virtualization technologies. His proficiency in application testing, database administration, and data cleansing ensures seamless execution of complex projects.
What sets Denis apart is his comprehensive understanding of Business and Systems Analysis technologies, honed through involvement in all phases of the Software Development Lifecycle (SDLC). From meticulous requirements gathering to precise analysis, innovative design, rigorous development, thorough testing, and successful implementation, he has consistently delivered exceptional results.
Throughout his career, he has taken on multifaceted roles, from leading technical project management teams to owning solutions that drive operational excellence. His conscientious and proactive approach is unwavering, whether he is working independently or collaboratively within a team. His ability to connect with colleagues on a personal level underscores his commitment to fostering a harmonious and productive workplace environment.
Date: May 29, 2024
Tags: Information Security, ISO/IEC 27001, ISO/IEC 42001, Artificial Intelligence, GDPR
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This presentation includes basic of PCOS their pathology and treatment and also Ayurveda correlation of PCOS and Ayurvedic line of treatment mentioned in classics.
3. DNA: The Genetic Material
• Search for genetic material---is it composed of nucleic acid or
protein/DNA or RNA?
• Griffith’s Transformation Experiment
• Avery’s Transformation Experiment
• Hershey-Chase Bacteriophage Experiment
• Nucleotides - composition and structure
• Double-helix model of DNA - Watson & Crick
• Organization of DNA/RNA in chromosomes
• Prokaryotes
• Eukaryotes
4. Search for the genetic material:
1. Stable source of information
2. Ability to replicate accurately
3. Capable of change
Timeline of events:
• 1890 Weismann - substance in the cell nuclei controls development.
• 1900 Chromosomes shown to contain hereditary information,
later shown to be composed of protein & nucleic acids.
• 1928 Griffith’s Transformation Experiment (incorrectly guessed protein!)
• 1944 Avery’s Transformation Experiment (DNA not RNA)
• 1953 Hershey-Chase Bacteriophage Experiment (DNA not protein)
• 1953 Watson & Crick propose double-helix model of DNA
• 1956 First demonstration that RNA is viral genetic material.
5. Fig. 2.2: Frederick Griffith’s Transformation Experiment - 1928
“transforming principle” demonstrated with Streptococcus pneumoniae
Griffith hypothesized that the transforming agent was a “IIIS” protein.
But this was only a guess, and Griffith turned out to be wrong.
7. Bacteriophage = Virus that attacks
bacteria and replicates by
invading a living cell and using
the cell’s molecular machinery.
Fig. 2.4
Structure of T2 phage
Bacteriophages
are composed of
DNA & protein
Hershey-Chase Bacteriophage Experiment - 1953
9. 1. T2 bacteriophage is composed
of DNA and proteins:
2. Set-up two replicates:
• Label DNA with 32P
• Label Protein with 35S
3. Infected E. coli bacteria with
two types of labeled T2
4. 32P is discovered within the
bacteria and progeny phages,
whereas 35S is not found within
the bacteria but released with
phage ghosts.
Fig. 2.6: Hershey-Chase Bacteriophage Experiment - 1953
Alfred Hershey
10. Conclusions about these early experiments:
Griffith 1928 & Avery 1944:
DNA (not RNA) is transforming agent.
Hershey-Chase 1953:
DNA (not protein) is the genetic material.
Gierer & Schramm 1956/Fraenkel-Conrat & Singer 1957:
RNA (not protein) is genetic material of some viruses, but no known
prokaryotes or eukaryotes use RNA as their genetic material.
Alfred Hershey
Nobel Prize in Physiology or Medicine
1969
11. Nucleotide = monomers that make up DNA and RNA (Figs. 2.8)
Three components
1. Pentose (5-carbon) sugar
DNA = deoxyribose
RNA = ribose
(compare 2’ carbons)
2. Nitrogenous base
Purines (2 rings)
Adenine
Guanine
Pyrimidines (1 ring)
Cytosine
Thymine (DNA)
Uracil (RNA)
3. Phosphate group attached to 5’ carbon
12. Nucleotides are linked by phosphodiester bonds to form polynucleotides.
Phosphodiester bond
Covalent bond between the phosphate group (attached to 5’ carbon) of
one nucleotide and the 3’ carbon of the sugar of another nucleotide.
This bond is very strong, and for this reason DNA is remarkably stable.
DNA can be boiled and even autoclaved without degrading!
No kidding, you can autoclave a mouse and get good PCR!
5’ and 3’
The ends of the DNA or RNA chain are not the same. One end of the
chain has a 5’ carbon and the other end has a 3’ carbon.
14. Structure of DNA
James D. Watson/Francis H. Crick 1953 proposed the Double Helix
Model based on two sources of information:
1. Base composition studies of Erwin Chargaff (Chargaff’s Rules)
• indicated double-stranded DNA consists of ~50% purines
(A,G) and ~50% pyrimidines (T, C)
• amount of A = amount of T and amount of G = amount of C
• %GC content varies from organism to organism
Examples: %A %T %G %C %GC
Homo sapiens 31.0 31.5 19.1 18.4 37.5
Zea mays 25.6 25.3 24.5 24.6 49.1
Drosophila 27.3 27.6 22.5 22.5 45.0
Aythya americana 25.8 25.8 24.2 24.2 48.4
15. Structure of DNA
James D. Watson/Francis H. Crick 1953 proposed the Double Helix
Model based on two sources of information:
2. X-ray diffraction studies by Rosalind Franklin & Maurice Wilkins
Conclusion-DNA is a helical structure with
distinctive regularities, 0.34 nm & 3.4 nm.
Fig. 2.11
16. Double Helix Model of DNA: Six main features
1. Two polynucleotide chains wound in a right-handed (clockwise)
double-helix.
2. Nucleotide chains are anti-parallel: 5’ 3’
3’ 5’
3. Sugar-phosphate backbones are on the outside of the double
helix, and the bases are oriented towards the central axis.
4. Complementary base pairs from opposite strands are bound
together by weak hydrogen bonds.
A pairs with T (2 H-bonds), and G pairs with C (3 H-bonds).
5’-TATTCCGA-3’
3’-ATAAGGCT-5’
5. Base pairs are 0.34 nm apart. One complete turn of the helix
requires 3.4 nm (10 bases/turn).
6. Sugar-phosphate backbones are not equally-spaced, resulting
in major and minor grooves.
18. Fig. 2.12
Type B-DNA
Other DNA forms
include:
A-DNA:
Right-handed double
helix with 11 bases
per turn; shorter and
wider at 2.2 nm
diameter. Exists in
some DNA-protein
complexes.
Z-DNA:
Left-handed double
helix with 12 bases
per turn; longer and
thinner at 1.8 nm
diameter.
19. Type A, B, and Z conformations of DNA
Fig. 2.14
21. Yeast Alanine tRNA
RNA possesses uracil (U) not thymine (T)
A pairs with U and C pairs with G
Examples:
mRNA messenger RNA
tRNA transfer RNA
rRNA ribosomal RNA
snRNA small nuclear RNA
miRNA micro RNA
siRNA small interfering RNA
RNA secondary structure:
single-stranded
Function in
transcription
(RNA processing)
and translation
22. Organization of DNA/RNA in chromosomes
Genome = chromosome or set of chromosomes that contains all the
DNA an organism (or organelle) possesses
Viral chromosomes 1. single or double-stranded DNA or RNA
2. circular or linear
3. surrounded by proteins
TMV T2 bacteriophage bacteriophage
Prokaryotic chromosomes
1. most contain one double-stranded circular
DNA chromosome
2. others consist of one or more chromosomes
and are either circular or linear
3. typically arranged in arranged in a dense
clump in a region called the nucleoid
23. Problem:
Measured linearly, the Escherichia coli genome (4.6 Mb) would be 1,000
times longer than the E. coli cell.
The human genome (3.4 Gb) would be 2.3 m long if stretched linearly.
Fig. 2.15
Chromosome released
from lysed E. coli cell.
24. Eukaryotic chromosome structure
Chromatin complex of DNA and chomosomal proteins
~ twice as much protein as DNA
Two major types of proteins:
1. Histones abundant, basic proteins with a positive charge
that bind to DNA
5 main types: H1, H2A, H2B, H3, H4
~equal in mass to DNA
evolutionarily conserved
2. Non-histones all the other proteins associated with DNA
differ markedly in type and structure
amounts vary widely
>> 100% DNA mass
<< 50% DNA mass
25. Packing of DNA into chromosomes:
1. Level 1 Winding of DNA around histones to create a
nucleosome structure.
2. Level 2 Nucleosomes connected by
strands of linker DNA like
beads on a string.
3. Level 3 Packaging of nucleosomes into
30-nm chromatin fiber.
4. Level 4 Formation of looped domains.
See Fig. 2.20
28. Fig. 2.21 - Metaphase chromosome depleted of histones maintains its
shape with a nonhistone protein scaffold.
29. More about genome size:
C value = total amount of DNA in the haploid (1N) genome
Varies widely from species to species and shows no simple
relationship to structural or organizational complexity.
Examples C value (bp)
48,502
T4 168,900
HIV-1 9,750
E. Coli 4,639,221
Lilium formosanum 36,000,000,000
Zea mays 5,000,000,000
Amoeba proteus 290,000,000,000
Drosophila melanogaster 180,000,000
Mus musculus 3,454,200,000
Canis familiaris 3,355,500,000
Equus caballus 3,311,000,000
Homo sapiens 3,400,000,000