This document provides an introduction to nuclear magnetic resonance (NMR) spectroscopy. It discusses:
1) How NMR spectroscopy uses radio waves and magnetic fields to determine the structure of organic molecules. The two most common types are 1H NMR and 13C NMR.
2) In an NMR experiment, nuclei such as 1H and 13C can absorb energy and "flip" their spin when radio waves match their energy difference in magnetic fields.
3) NMR spectra provide information on the number, position, intensity, and splitting of peaks which reveal details about a molecule's carbon-hydrogen framework.
In this slide contains instrumentation of Fourier-Transform Nuclear Magnetic Resonance (FT-NMR).
Presented by: P. Venkatesh. (Department of pharmaceutical analysis).
RIPER, anantpur.
In this slide contains instrumentation of Fourier-Transform Nuclear Magnetic Resonance (FT-NMR).
Presented by: P. Venkatesh. (Department of pharmaceutical analysis).
RIPER, anantpur.
PRINCIPLES of FT-NMR & 13C NMR
Fourier Transform
FOURIER TRANSFORM NMR SPECTROSCOPY
THEORY OF FT-NMR
13C NMR SPECTROSCOPY
Principle
Why C13-NMR is required though we have H1-NMR?
CHARACTERISTIC FEATURES OF 13 C NMR
Chemical Shifts
NUCLEAR OVERHAUSER ENHANCEMENT
Short-Comings of 13C-NMR Spectra
This is regarding the Fourier Transform NMR helpful for the analysis in the Pharmaceutical field and this is helpful to the Masters students as this topic is in the syllabus and the presentation gives the complete and detail idea of various aspects of FT-NMR.
NMR Instrumentation
ppt
Magnet
Permanent and conventional electromagnets
The Magnetic Field Sweep
Sweep Generator
frequency sweep method
field sweep method
The Sample Holder
The Sample Probe
Radio Frequency Generator
Oscillator
Radio Frequency Receiver
Amplifier
The Signal Detector and Recording System
NMR Instrumentation
ppt
Magnet
Permanent and conventional electromagnets
The Magnetic Field Sweep
Sweep Generator
frequency sweep method
field sweep method
The Sample Holder
The Sample Probe
Radio Frequency Generator
Oscillator
Radio Frequency Receiver
Amplifier
The Signal Detector and Recording System
NMR- Diamagnetic Anisotropy and its effect on chemical shiftD.R. Chandravanshi
The shift in the position of the NMR region resulting from the shielding and deshielding by electrons is called chemical shift.
When a proton is present inside the magnetic field more close to an electro positive atom more applied magnetic field is required to cause excitation. This effect is called shielding effect.
When a proton is present outside the magnetic field close to a electronegative atom less applied magnetic field is required to cause excitation . This effect is called deshielding effect
In this slides contains principle and instrumentation of Differential Scanning Calorimeter (DSC).
Presented by: N Poojitha. (Department of pharmaceutics),
RIPER, anantapur.
In this slide contains Interference In Atomic Absorption Spectroscopy and applications.
Presented by: Shaik Gouse ul azam. ( department of pharmaceutical analysis.)
RIPER, anantpur.
PRINCIPLES of FT-NMR & 13C NMR
Fourier Transform
FOURIER TRANSFORM NMR SPECTROSCOPY
THEORY OF FT-NMR
13C NMR SPECTROSCOPY
Principle
Why C13-NMR is required though we have H1-NMR?
CHARACTERISTIC FEATURES OF 13 C NMR
Chemical Shifts
NUCLEAR OVERHAUSER ENHANCEMENT
Short-Comings of 13C-NMR Spectra
This is regarding the Fourier Transform NMR helpful for the analysis in the Pharmaceutical field and this is helpful to the Masters students as this topic is in the syllabus and the presentation gives the complete and detail idea of various aspects of FT-NMR.
NMR Instrumentation
ppt
Magnet
Permanent and conventional electromagnets
The Magnetic Field Sweep
Sweep Generator
frequency sweep method
field sweep method
The Sample Holder
The Sample Probe
Radio Frequency Generator
Oscillator
Radio Frequency Receiver
Amplifier
The Signal Detector and Recording System
NMR Instrumentation
ppt
Magnet
Permanent and conventional electromagnets
The Magnetic Field Sweep
Sweep Generator
frequency sweep method
field sweep method
The Sample Holder
The Sample Probe
Radio Frequency Generator
Oscillator
Radio Frequency Receiver
Amplifier
The Signal Detector and Recording System
NMR- Diamagnetic Anisotropy and its effect on chemical shiftD.R. Chandravanshi
The shift in the position of the NMR region resulting from the shielding and deshielding by electrons is called chemical shift.
When a proton is present inside the magnetic field more close to an electro positive atom more applied magnetic field is required to cause excitation. This effect is called shielding effect.
When a proton is present outside the magnetic field close to a electronegative atom less applied magnetic field is required to cause excitation . This effect is called deshielding effect
In this slides contains principle and instrumentation of Differential Scanning Calorimeter (DSC).
Presented by: N Poojitha. (Department of pharmaceutics),
RIPER, anantapur.
In this slide contains Interference In Atomic Absorption Spectroscopy and applications.
Presented by: Shaik Gouse ul azam. ( department of pharmaceutical analysis.)
RIPER, anantpur.
You will find here all the elements presented by the CENAPT team ( Drs. Guido Pauli and Charlotte Simmler) and pertaining to the NMR workshop at the American Society of Pharmacognosy (ASP 2017, Portland Oregon).
These slides summarize the different steps related to the implementation of quantitative NMR for purity analysis.
Part of Lecture series on EE646, Fuzzy Theory & Applications delivered by me during First Semester of M.Tech. Instrumentation & Control, 2012
Z H College of Engg. & Technology, Aligarh Muslim University, Aligarh
Reference Books:
1. T. J. Ross, "Fuzzy Logic with Engineering Applications", 2/e, John Wiley & Sons,England, 2004.
2. Lee, K. H., "First Course on Fuzzy Theory & Applications", Springer-Verlag,Berlin, Heidelberg, 2005.
3. D. Driankov, H. Hellendoorn, M. Reinfrank, "An Introduction to Fuzzy Control", Narosa, 2012.
Please comment and feel free to ask anything related. Thanks!
It would be use full to All Needy People. It involve information about NMR Spectroscopy ( a spectroscopic techniques), factors influencing , proton NMR and their applications of NMR as well as Nuclear magnetic imaging.
Theory of NMR, nuclear magnetic resonance, instrumentation, solvents, chemical shift, photon NMR, spin coupling, coupling constant and applications.
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Instructions for Submissions thorugh G- Classroom.pptxJheel Barad
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Synthetic Fiber Construction in lab .pptxPavel ( NSTU)
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A Strategic Approach: GenAI in EducationPeter Windle
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Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
Biological screening of herbal drugs: Introduction and Need for
Phyto-Pharmacological Screening, New Strategies for evaluating
Natural Products, In vitro evaluation techniques for Antioxidants, Antimicrobial and Anticancer drugs. In vivo evaluation techniques
for Anti-inflammatory, Antiulcer, Anticancer, Wound healing, Antidiabetic, Hepatoprotective, Cardio protective, Diuretics and
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June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
Letter from the Congress of the United States regarding Anti-Semitism sent June 3rd to MIT President Sally Kornbluth, MIT Corp Chair, Mark Gorenberg
Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
This Congress will not stand idly by and allow an environment hostile to Jewish students to persist. The House believes that your institution is in violation of Title VI of the Civil Rights Act, and the inability or
unwillingness to rectify this violation through action requires accountability.
Postsecondary education is a unique opportunity for students to learn and have their ideas and beliefs challenged. However, universities receiving hundreds of millions of federal funds annually have denied
students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
• The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
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• The Committee on Ways and Means has been investigating several universities since November 15, 2023, when the Committee held a hearing entitled From Ivory Towers to Dark Corners: Investigating the Nexus Between Antisemitism, Tax-Exempt Universities, and Terror Financing. The Committee followed the hearing with letters to those institutions on January 10, 202
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1. 1
THEORY OF
NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY
By
Sayyad Ali Khan. Ph.D. Scholar
Reg. # FA13-R60-002/ATD
COMSATS Abbottabad Pakistan
2. 2
Nuclear Magnetic Resonance Spectroscopy
• Nuclear magnetic resonance spectroscopy is a powerful
analytical technique used to characterize organic molecules by
identifying carbon-hydrogen frameworks within molecules.
• Two common types of NMR spectroscopy are used to
characterize organic structure: 1
H NMR is used to determine
the type and number of H atoms in a molecule; 13
C NMR is
used to determine the type of carbon atoms in the molecule.
• The source of energy in NMR is radio waves which have long
wavelengths, and thus low energy and frequency.
• When low-energy radio waves interact with a molecule, they
can change the nuclear spins of some elements, including 1
H
and 13
C.
Introduction to NMR Spectroscopy
3. 3
• When a charged particle such as a proton spins on its axis, it
creates a magnetic field. Thus, the nucleus can be considered
to be a tiny bar magnet.
• Normally, these tiny bar magnets are randomly oriented in
space. However, in the presence of a magnetic field B0, they
are oriented with or against the applied M. field. More nuclei
are oriented with the applied field because this arrangement is
lower in energy.
Introduction to NMR Spectroscopy
4. 4
• In a magnetic field, there are now two energy states for a
proton: a lower energy state with the nucleus aligned in the
same direction as B0, and a higher energy state in which the
nucleus aligned against B0.
• When an external energy source (hν) that matches the energy
difference (∆E) between these two states is applied, energy is
absorbed, causing the nucleus to “spin flip” from one
orientation to another.
• The energy difference between these two nuclear spin states
corresponds to the low frequency RF region of the
electromagnetic spectrum.
Introduction to NMR Spectroscopy
5. 5
• Thus, two variables characterize NMR: an applied magnetic field B0, the
strength of which is measured in tesla (T), and the frequency ν of radiation
used for resonance, measured in hertz (Hz), or megahertz (MHz)—(1 MHz
= 106
Hz).
Introduction to NMR Spectroscopy
Radiofrequency (RF) frequency ranges from 3 kilohertz (kHz) to 300
Megahertz (MHz), and microwave (MW) radiation are electromagnetic
radiation from 300 MHz to 300 gigahertz (GHz).
6. 6
• The frequency of the light needed for resonance and the
applied magnetic field strength are proportionally related:
• NMR spectrometers are referred to as 300 MHz instruments,
500 MHz instruments, and so forth, depending on the
frequency of the RF radiation used for resonance.
• These spectrometers use very powerful magnets to create a
small but measurable energy difference between two possible
spin states.
Introduction to NMR Spectroscopy
8. 8
• Protons in different environments absorb at slightly
different frequencies, so they are distinguishable by NMR.
• The frequency at which a particular proton absorbs is
determined by its electronic environment.
• Modern NMR spectrometers use a constant magnetic field
strength B0, and then a narrow range of frequencies is
applied to achieve the resonance of all protons.
• Only nuclei that contain odd mass numbers (such as 1
H, 13
C,
19
F and 31
P) or odd atomic numbers (such as 2
H and 14
N) give
rise to NMR signals.
Introduction to NMR Spectroscopy
9. 9
• An NMR spectrum is a plot of the intensity of a peak against
its chemical shift, measured in parts per million (ppm).
1
H NMR—The Spectrum
10. 10
• NMR absorptions generally appear as sharp peaks.
• Increasing chemical shift is plotted from right to left.
• Most protons absorb between 0-10 ppm.
• The terms “upfield” and “downfield” describe the relative
location of peaks. Upfield means to the right. Downfield means
to the left.
• NMR absorptions are measured relative to the position of a
reference peak at 0 ppm on the δ scale due to tetramethylsilane
(TMS). TMS is a volatile inert compound that gives a single peak
upfield from typical NMR absorptions and taken as “0”.
1
H NMR—The Spectrum
11. 11
• The chemical shift of the x axis gives the position of an NMR signal,
measured in ppm, according to the following equation:
1
H NMR—The Spectrum
• Four different features of a 1
H NMR spectrum provide information
about a compound’s structure:
a. Number of signals
b. Position of signals
c. Intensity of signals.
d. Spin-spin splitting of signals.
12. 12
• The number of NMR signals equals the number of different types of
protons in a compound.
• Protons in different environments give different NMR signals.
• Equivalent protons give the same NMR signal.
1
H NMR—Number of Signals
• To determine equivalent protons in cycloalkanes and alkenes, one
should always draw all bonds to hydrogen.
13. 13
• In comparing two H atoms on a ring or double bond, two
protons are equivalent only if they are cis (or trans) to the
same groups.
1
H NMR—Number of Signals
14. 14
• In the vicinity of the nucleus, the magnetic field generated by the
circulating electron decreases the external magnetic field that the
proton “feels”.
• Since the electron experiences a lower magnetic field strength, it needs
a lower frequency to achieve resonance. Lower frequency is to the right
in an NMR spectrum, toward a lower chemical shift, so shielding shifts
the absorption upfield.
1
H NMR—Position of Signals
15. 15
• The less shielded the nucleus becomes, the more of the
applied magnetic field (B0) it feels.
• This deshielded nucleus experiences a higher magnetic field
strength, i.e. it needs a higher frequency to achieve resonance.
• Higher frequency is to the left in an NMR spectrum, toward
higher chemical shift—so deshielding shifts an absorption
downfield.
• Protons near electronegative atoms are deshielded, so they
absorb downfield.
1
H NMR—Position of Signals
17. 17
• The chemical shift of a C—H bond increases with increasing
alkyl substitution.
1
H NMR—Chemical Shift Values
18. 18
• In a magnetic field, the six π electrons in benzene circulate around the
ring.
• The magnetic field induced by these moving electrons reinforces the
applied magnetic field in the vicinity of the protons.
• The protons thus feel a stronger magnetic field and a higher frequency
is needed for resonance. Thus they are deshielded and absorb
downfield.
1
H NMR—Chemical Shift
22. 22
• Spin-spin splitting occurs only between nonequivalent
protons on the same carbon or adjacent carbons.
1
H NMR—Spin-Spin Splitting
• When placed in an applied electric field, (B0), the adjacent
proton (—CH2Br) can be aligned with (↑) or against (↓) B0.
• Thus, the absorbing CH2 protons feel two slightly different
magnetic fields.
• Since the absorbing protons feel two different magnetic
fields, they absorb at two different frequencies in the NMR
spectrum, thus splitting a single absorption into a doublet.
23. 23
1
H NMR—Spin-Spin Splitting
The frequency difference, measured in Hz between two peaks of the
doublet is called the coupling constant, J.
24. 24
1
H NMR—Spin-Spin Splitting
consider how a triplet arises:
• When placed in an applied magnetic field (B0), the adjacent
protons Ha and Hb can each be aligned with (↑) or against (↓)
B0.
• Thus, the absorbing proton feels three slightly different
magnetic fields.
26. 26
1
H NMR—Spin-Spin Splitting
Three general rules describe the splitting patterns commonly seen
in the 1
H NMR spectra of organic compounds.
1. Equivalent protons do not split each other’s signals.
2. A set of ‘n’ non-equivalent protons splits the signal of a nearby
proton into n + 1 peaks.
3. Splitting is observed for nonequivalent protons on the same
carbon or adjacent carbons.
If Ha and Hb are not equivalent, splitting is observed when:
28. 28
13
C NMR
13
C Spectra are easier to analyze than 1
H spectra because the signals are not
split. Each type of carbon atom appears as a single peak.
29. 29
13
C NMR
• The lack of splitting in a 13
C spectrum is a consequence of the low natural
abundance of 13
C.
• As splitting occurs when two NMR active nuclei—like two protons are
close to each other. Because of the low natural abundance of 13
C nuclei
(1.1% of 12
C ), the chance of two 13
C nuclei being bonded to each other is
very small (0.01%), and so no carbon-carbon splitting is observed.
• A 13
C NMR signal can also be split by nearby protons. This 1
H-13
C splitting
is usually eliminated from the spectrum by using an instrumental
technique so that every peak in a 13
C NMR spectrum appears as a singlet.
• The two features of a 13
C NMR spectrum that provide the most structural
information are the number of signals observed and the chemical shifts
of those signals.
30. 30
13
C NMR—Number of Signals
• The number of signals in a 13
C spectrum gives the number of different
types of carbon atoms in a molecule.
• Because 13
C NMR signals are not split, the number of signals equals the
number of lines in the 13
C spectrum.
• In contrast to the 1
H NMR situation, peak intensity is not proportional to
the number of absorbing carbons, so 13
C NMR signals are not integrated.
31. 31
13
C NMR—Position of Signals
• In contrast to the small range of chemical shifts in 1
H NMR (1-10
ppm usually), 13
C NMR absorptions occur over a much broader
range (0-220 ppm).
• The chemical shifts of carbon atoms in 13
C NMR depend on the
same effects as the chemical shifts of protons in 1
H NMR.