This document discusses mycorrhizal induced resistance (MIR) and plant innate immunity. It describes the different types of mycorrhizal associations including arbuscular mycorrhizae. MIR occurs in four phases and involves modulation of the plant immune system through changes in hormones like jasmonic acid and salicylic acid. Arbuscular mycorrhizal fungi can prime the jasmonic acid pathway in plants to induce a stronger defense response against pathogens. Gene expression is also altered in mycorrhizal plants to produce antifungal proteins that enhance resistance.
This document provides information about mycorrhizae, which is a symbiotic relationship between fungi and plant roots. It defines mycorrhizae and describes the two main types: ectomycorrhizae and endomycorrhizae. Ectomycorrhizae are formed between fungi and the roots of about 10% of plant families, mainly woody plants. They involve a fungal sheath surrounding the root. Endomycorrhizae penetrate the root cells and include arbuscular mycorrhizae, the most common type, characterized by structures called arbuscules and vesicles. The relationship benefits both the plant, which receives increased nutrient and water absorption, and the fungus, which receives
Activation of natural host defence by elicitors for management of post harves...Vinod Upadhyay
India suffers post-harvest losses of over $2 billion annually due to spoilage of 30% of fruits and vegetables after harvesting. Elicitors are compounds that can activate natural host defense responses in plants. Some examples of elicitors that have been shown to reduce post-harvest diseases include salicylic acid, methyl jasmonate, harpin, and oligandrin. Elicitors work by priming the plant's defenses through mechanisms like reactive oxygen species production, phytoalexin production, and hypersensitive response. The effectiveness of elicitors can depend on factors like cultivar genotype, environmental conditions, and whether they are combined with other treatments like fungicides or biocontrol agents.
This document provides an overview of soil-microbe-plant interactions in the rhizosphere. It discusses how plant roots release exudates that attract microbes and influence the rhizosphere environment. Microbes can benefit plants through nutrient cycling, hormone production, and pathogen inhibition. Environmental factors like temperature and moisture impact root exudates and the rhizosphere microbiome. Plant genotype and traits also shape interactions with soil microbes. The rhizosphere enhances soil quality through physical and nutrient dynamics effects. A diverse microbiome can boost plant growth, and pathogenic fungi can negatively impact plants via soil feedbacks. In conclusion, the rhizosphere is a zone where soil biology and chemistry are influenced by roots and microbes through complex
root microbial interaction for crop improvement seminar ppt Balaji Rathod
This document discusses root-microbial interactions and how they impact crop improvement. It covers how plant root exudates can influence both positive and negative interactions with other plants and microbes. On the plant-plant side, exudates can facilitate resource competition, allelopathy, or parasitic relationships. Positively, they may induce defenses in neighboring plants. With microbes, exudates enable symbiotic relationships like nitrogen fixation and mycorrhizal associations, but can also have antimicrobial effects against pathogens. A better understanding of these below-ground processes could help develop strategies to enhance soil health and crop yields.
Biological control of plant pathogens involves using beneficial microorganisms to suppress disease-causing pathogens. Trichoderma fungi are commonly used as biological control agents against soilborne fungal pathogens. They can control pathogens through antibiosis, nutrient competition, and destructive mycoparasitism. For successful biocontrol, the agent must be effective, able to compete and persist in the environment, be produced inexpensively, and applied in a way that allows it to function. Commercial Trichoderma products are used as soil treatments or seed coatings to protect plant roots from diseases caused by fungi like Pythium, Rhizoctonia, and Fusarium. Future developments may involve engineering crops with transgenes from biocontrol
Plant microbe interaction by dr. ashwin chekeAshwin Cheke
PLANT MICROBE – INTERACTIONS AND THEIR MUTUAL BENEFITS IN ENHANCING SOIL HEALTH AND AGRICULTURAL PRODUCTION ,
IT ALSO INCREASE CROP PRODUCTIVITY AND IMPROVE SOIL HEALTH
This document discusses different types of mycorrhizal fungi and their importance in solubilizing and mobilizing phosphorus for plants. There are two main types - ectomycorrhizae and endomycorrhizae. Ectomycorrhizal fungi form a Hartig net around root cells and extend hyphae into the soil to uptake phosphorus and other nutrients. Endomycorrhizal fungi are mainly Glomeromycota that form arbuscules and vesicles inside root cells to transport phosphorus and nutrients from the soil to the plant. Mycorrhizal associations are beneficial as they help plants uptake nutrients, water and protect roots.
This document provides information about mycorrhizae, which is a symbiotic relationship between fungi and plant roots. It defines mycorrhizae and describes the two main types: ectomycorrhizae and endomycorrhizae. Ectomycorrhizae are formed between fungi and the roots of about 10% of plant families, mainly woody plants. They involve a fungal sheath surrounding the root. Endomycorrhizae penetrate the root cells and include arbuscular mycorrhizae, the most common type, characterized by structures called arbuscules and vesicles. The relationship benefits both the plant, which receives increased nutrient and water absorption, and the fungus, which receives
Activation of natural host defence by elicitors for management of post harves...Vinod Upadhyay
India suffers post-harvest losses of over $2 billion annually due to spoilage of 30% of fruits and vegetables after harvesting. Elicitors are compounds that can activate natural host defense responses in plants. Some examples of elicitors that have been shown to reduce post-harvest diseases include salicylic acid, methyl jasmonate, harpin, and oligandrin. Elicitors work by priming the plant's defenses through mechanisms like reactive oxygen species production, phytoalexin production, and hypersensitive response. The effectiveness of elicitors can depend on factors like cultivar genotype, environmental conditions, and whether they are combined with other treatments like fungicides or biocontrol agents.
This document provides an overview of soil-microbe-plant interactions in the rhizosphere. It discusses how plant roots release exudates that attract microbes and influence the rhizosphere environment. Microbes can benefit plants through nutrient cycling, hormone production, and pathogen inhibition. Environmental factors like temperature and moisture impact root exudates and the rhizosphere microbiome. Plant genotype and traits also shape interactions with soil microbes. The rhizosphere enhances soil quality through physical and nutrient dynamics effects. A diverse microbiome can boost plant growth, and pathogenic fungi can negatively impact plants via soil feedbacks. In conclusion, the rhizosphere is a zone where soil biology and chemistry are influenced by roots and microbes through complex
root microbial interaction for crop improvement seminar ppt Balaji Rathod
This document discusses root-microbial interactions and how they impact crop improvement. It covers how plant root exudates can influence both positive and negative interactions with other plants and microbes. On the plant-plant side, exudates can facilitate resource competition, allelopathy, or parasitic relationships. Positively, they may induce defenses in neighboring plants. With microbes, exudates enable symbiotic relationships like nitrogen fixation and mycorrhizal associations, but can also have antimicrobial effects against pathogens. A better understanding of these below-ground processes could help develop strategies to enhance soil health and crop yields.
Biological control of plant pathogens involves using beneficial microorganisms to suppress disease-causing pathogens. Trichoderma fungi are commonly used as biological control agents against soilborne fungal pathogens. They can control pathogens through antibiosis, nutrient competition, and destructive mycoparasitism. For successful biocontrol, the agent must be effective, able to compete and persist in the environment, be produced inexpensively, and applied in a way that allows it to function. Commercial Trichoderma products are used as soil treatments or seed coatings to protect plant roots from diseases caused by fungi like Pythium, Rhizoctonia, and Fusarium. Future developments may involve engineering crops with transgenes from biocontrol
Plant microbe interaction by dr. ashwin chekeAshwin Cheke
PLANT MICROBE – INTERACTIONS AND THEIR MUTUAL BENEFITS IN ENHANCING SOIL HEALTH AND AGRICULTURAL PRODUCTION ,
IT ALSO INCREASE CROP PRODUCTIVITY AND IMPROVE SOIL HEALTH
This document discusses different types of mycorrhizal fungi and their importance in solubilizing and mobilizing phosphorus for plants. There are two main types - ectomycorrhizae and endomycorrhizae. Ectomycorrhizal fungi form a Hartig net around root cells and extend hyphae into the soil to uptake phosphorus and other nutrients. Endomycorrhizal fungi are mainly Glomeromycota that form arbuscules and vesicles inside root cells to transport phosphorus and nutrients from the soil to the plant. Mycorrhizal associations are beneficial as they help plants uptake nutrients, water and protect roots.
Soil organic matter has long been recognized as one of the most important components in maintaining soil fertility, soil quality, and agricultural sustainability. The soil zone strongly influenced by plant roots, the rhizosphere, plays an important role in regulating soil organic matter decomposition and nutrient cycling. Processes that are largely controlled or directly influenced by roots are often referred to as rhizosphere processes. These processes may include exudation of soluble compounds, water uptake, nutrient mobilization by roots and microorganisms, rhizosphere-mediated soil organic matter decomposition, and the subsequent release of CO2 through respiration. Rhizosphere processes are major gateways for nutrients and water. At the global scale, rhizosphere processes utilize approximately 50% of the energy fixed by photosynthesis in terrestrial ecosystems, contribute roughly 50% of the total CO2 emitted from terrestrial ecosystems, and mediate virtually all aspects of nutrient cycling. Therefore, plant roots and their rhizosphere interactions are at the center of many ecosystem processes. However, the linkage between rhizosphere processes and soil organic matter decomposition is not well understood. Because of the lack of appropriate methods, rates of soil organic matter decomposition are commonly assessed by incubating soil samples in the absence of vegetation and live roots with an implicit assumption that rhizosphere processes have little impact on the results. Our recent studies have overwhelmingly proved that this implicit assumption is often invalid, because the rate of soil organic matter decomposition can be accelerated by as much as 380% or inhibited by as much as 50% by the presence of live roots. The rhizosphere effect on soil organic matter decomposition is often large in magnitude and significant in mediating plant-soil interactions.
The document discusses various types of biofertilizers including bacterial, fungal, algal and aquatic fern biofertilizers. It describes key bacterial biofertilizers such as Rhizobium, Azospirillum, Azotobacter and their roles in nitrogen fixation. It also discusses fungal biofertilizers including vesicular arbuscular mycorrhiza fungi and their mechanism of enhancing nutrient transfer. The mass production process of different biofertilizers including fermentation and use of suitable carrier materials is summarized. Algae are also mentioned as a common free-living nitrogen fixing biofertilizer used in rice fields.
the uploaded subject "introduction to plant pathogens/ pathology" is deals with plant diseases which is caused by plant pathogens like fungi, bacteria, virus, phytoplasma, spiroplasma and viroids etc. and also provides basic information regarding all plant pathogens and their characters and life cycles
Blue-green algae, also known as cyanobacteria, are photosynthetic bacteria that produce oxygen. They possess chlorophyll and can fix nitrogen. Azolla is an aquatic fern that has a symbiotic relationship with nitrogen-fixing cyanobacteria. It grows easily and can be used as green manure to improve soil nutrition. Both blue-green algae and Azolla are important for agriculture - blue-green algae fix nitrogen while Azolla acts as a biofertilizer when used in rice fields and on other crops.
This document discusses induced systemic resistance (ISR) in plants. It provides historical context on studies of induced resistance dating back to the late 1800s. ISR is defined as a phenomenon where treatment with certain chemicals or pathogens activates a plant's defenses throughout the plant. Key findings include:
- ISR is activated by rhizobacteria and involves jasmonic acid and ethylene signaling rather than salicylic acid signaling as in systemic acquired resistance.
- Several bacteria, fungi, chemicals, and elicitors are reported to induce ISR through different signaling pathways and defense responses.
- Further research is needed to fully understand ISR signaling and apply it effectively in fields to control plant diseases.
This document defines fungicides and related terms like fungistat and antisporulant. It classifies fungicides based on their mode of action (protectant, therapeutant, eradicatcant), general use (seed protectants, soil fungicides, foliage fungicides), and chemical composition (sulphur fungicides, copper fungicides, dithiocarbamates). Specific examples are provided for each classification along with the diseases they control.
“Any living organism supplying plant nutrients directly or indirectly is regarded as biofertilizer. They are not synthetically manufactured in factory.”
introduction to mycorrhizae and its role in P uptakeFari Rajput
This document discusses mycorrhiza and its role in phosphorus uptake by plants. It begins by defining mycorrhiza as a symbiotic relationship between plant roots and fungi, where the fungi help facilitate nutrient and water uptake in exchange for carbohydrates from the plant. There are two main types of mycorrhiza - ectomycorrhiza and endomycorrhiza. Mycorrhizal fungi take up nutrients from soil through a mycorrhizal pathway and transfer them to the plant, helping the plant access nutrients like phosphorus that are poorly mobile in soil. They do this through mechanisms like producing phosphatases and organic acids to solubilize inorganic and organic phosphorus sources, and storing
This document discusses different types of mycorrhizal associations between fungi and plant roots. The main types discussed are ectomycorrhizae, arbuscular mycorrhizae, orchid mycorrhizae, ericoid mycorrhizae, arbutoid mycorrhizae, and monotropoid mycorrhizae. These associations are typically mutualistic, with fungi helping plants uptake nutrients and water, and plants providing fungi with carbohydrates. Ectomycorrhizal fungi form sheaths around roots and penetrate the cortex, while arbuscular mycorrhizal fungi form structures called arbuscules and vesicles within root cells.
The document discusses biofungicides, which are biologically active substances derived from organisms that prevent and treat plant diseases. There are three main types of biofungicides: agricultural antibiotics produced through microbial fermentation, bacteria-based fungicides like Bacillus subtilis that inhibit fungi and promote growth, and fungal fungicides including Trichoderma species that control various plant pathogens. Biofungicides have advantages over chemical fungicides in that their active ingredients are naturally occurring microorganisms that are less toxic and help improve soil health.
Mechanism of disease control by endophytesPooja Bhatt
The document discusses alternative methods for pest management to address problems with chemical pesticides such as development of resistance and environmental contamination. It suggests that biological control using endophytic microorganisms is a promising alternative as endophytes have antagonistic properties against plant pathogens. Endophytes can inhibit pathogens through direct mechanisms such as hyperparasitism, competition, antibiosis, and lytic enzyme production or indirect induction of host plant resistance. Case studies provide examples of endophytes inhibiting fungal plant pathogens through siderophore production, parasitic growth, and antibiotic compounds.
The document discusses mycorrhizae and their potential as biocontrol agents. It describes the evolution and discovery of mycorrhizal fungi. There are different types of mycorrhizal associations based on the relationship with host plants. The document discusses the distribution of mycorrhizal fungi among host and non-host plants. It explains how vesicular arbuscular mycorrhizae (VAM) can act as biocontrol agents by suppressing soil-borne pathogens through various mechanisms like physiological alterations in host plants, competition for space and nutrients, and changes in root exudates. The mycorrhizal intensity was found to be higher in healthy plants compared to diseased plants in fields studied
This document discusses biosolubilization of insoluble phosphates in a fluidized bed bioreactor using immobilized microorganisms. Phosphate solubilizing bacteria and fungi were entrapped in calcium alginate and polyacrylamide gels and used to solubilize mineral phosphates in a fluidized bed bioreactor. This allows for reusability of the biocatalyst and control of reactions without contamination. The longevity of solubilization activity was tested through repeated batch experiments. Bioconversion using these immobilized microorganisms occurs at low temperatures and provides a sustainable way to extract and solubilize phosphates compared to conventional processes.
The document discusses systemic acquired resistance (SAR), which confers long-lasting protection against a broad spectrum of pathogens. SAR is induced by initial infection and involves the signaling molecule salicylic acid, leading to accumulation of pathogenesis-related proteins throughout the plant. Key regulators of SAR include NPR1, which is required for SAR, and salicylic acid, which is involved in transmitting the defense signal systemically.
phyllosphere is a dynamic rapidly changing area surrounding the germinating seed. there are two categories of microbes one is positively enhancing and negatively reducing the plant yield
This document discusses Azotobacter, a genus of nitrogen-fixing bacteria that can be used as a biofertilizer. It describes the key species of Azotobacter, their identifying characteristics, and their benefits to agriculture. Azotobacter promotes plant growth by fixing atmospheric nitrogen and producing plant hormones. It also functions as a biocontrol agent by suppressing plant pathogens. The document outlines Azotobacter's mode of action in plants and provides examples of increased crop yields and quality from its use as an inoculant. It also discusses the maintenance, selection, and mass production methods for Azotobacter cultures.
This document discusses phosphorus uptake by mycorrhizal fungi. It outlines two pathways for phosphorus uptake - the direct pathway where plants absorb phosphorus directly from the soil, and the mycorrhizal pathway where a symbiotic relationship with mycorrhizal fungi allows for greater phosphorus absorption. The mycorrhizal pathway involves mycorrhizal fungi extending their hyphae into the soil to absorb phosphorus, which is then transported to the plant. Mycorrhizal associations are significant as they improve plant nutrition and phosphorus solubility in soil.
The rhizosphere is the region of soil surrounding plant roots that is influenced by root secretions like mucilage, exudates, and lysates. It contains many microorganisms in complex relationships with the plant roots. Root secretions, collectively known as rhizodeposition, enrich the soil environment and stimulate microbial growth in the rhizosphere compared to bulk soil, as measured by the R:S ratio of microorganisms. Rhizodeposition includes a variety of organic compounds that influence soil nutrients and microbes.
Effect of Clariodeoglomusclariodeorum on morphology and abundant of carrot ro...AI Publications
The roothairs are important components of the root for absorbing nutrients for plants and also secreting the plant-produced secretes. Morphology and their number are influenced by various environmental and internal factors and are regulated by them. Mycorrhizal fungi are established through the root and their presence in the root can affect root’s physical and chemical properties.The aim of this study was to evaluate the effect of the presence of the fungus in the roots on the characteristics of the capillary roots.In order to remove the effect of other microorganisms on the results of the experiment, this experiment was performed in vitro on the roots of the carrot secondary phloem tissue culture. In this experiment, the fungus could affect the characteristics of the root hairs: their number (22% decrease in the root hairs number in the root hair area of the root) and their length (A decrease of 21.3% in the length of capillary roots in mycorrhizal plants). These changes in the characteristics of capillary roots were also caused by the presence of fungal structures in the roots as well as by the decrease in the production of strigolactones. In this study, changes in the production of strigolactones calculated by using their effect on seed germination of Phelipancheaegyptiaca.
Plants require essential nutrients for their growth and development that are mainly acquired from soil by their roots. Nutrient stress is an environmental condition that can seriously affect the production and quality of crop produce. Biofertilizers are the organisms (Bacteria, fungi, cyanobacteria, etc.) that enrich the nutrient quality of soil. Plants have a number of beneficial relationship with such organisms. Among these AM-Fungi are ubiquitous and form a mutuality relationship with roots of most plant species.
Soil organic matter has long been recognized as one of the most important components in maintaining soil fertility, soil quality, and agricultural sustainability. The soil zone strongly influenced by plant roots, the rhizosphere, plays an important role in regulating soil organic matter decomposition and nutrient cycling. Processes that are largely controlled or directly influenced by roots are often referred to as rhizosphere processes. These processes may include exudation of soluble compounds, water uptake, nutrient mobilization by roots and microorganisms, rhizosphere-mediated soil organic matter decomposition, and the subsequent release of CO2 through respiration. Rhizosphere processes are major gateways for nutrients and water. At the global scale, rhizosphere processes utilize approximately 50% of the energy fixed by photosynthesis in terrestrial ecosystems, contribute roughly 50% of the total CO2 emitted from terrestrial ecosystems, and mediate virtually all aspects of nutrient cycling. Therefore, plant roots and their rhizosphere interactions are at the center of many ecosystem processes. However, the linkage between rhizosphere processes and soil organic matter decomposition is not well understood. Because of the lack of appropriate methods, rates of soil organic matter decomposition are commonly assessed by incubating soil samples in the absence of vegetation and live roots with an implicit assumption that rhizosphere processes have little impact on the results. Our recent studies have overwhelmingly proved that this implicit assumption is often invalid, because the rate of soil organic matter decomposition can be accelerated by as much as 380% or inhibited by as much as 50% by the presence of live roots. The rhizosphere effect on soil organic matter decomposition is often large in magnitude and significant in mediating plant-soil interactions.
The document discusses various types of biofertilizers including bacterial, fungal, algal and aquatic fern biofertilizers. It describes key bacterial biofertilizers such as Rhizobium, Azospirillum, Azotobacter and their roles in nitrogen fixation. It also discusses fungal biofertilizers including vesicular arbuscular mycorrhiza fungi and their mechanism of enhancing nutrient transfer. The mass production process of different biofertilizers including fermentation and use of suitable carrier materials is summarized. Algae are also mentioned as a common free-living nitrogen fixing biofertilizer used in rice fields.
the uploaded subject "introduction to plant pathogens/ pathology" is deals with plant diseases which is caused by plant pathogens like fungi, bacteria, virus, phytoplasma, spiroplasma and viroids etc. and also provides basic information regarding all plant pathogens and their characters and life cycles
Blue-green algae, also known as cyanobacteria, are photosynthetic bacteria that produce oxygen. They possess chlorophyll and can fix nitrogen. Azolla is an aquatic fern that has a symbiotic relationship with nitrogen-fixing cyanobacteria. It grows easily and can be used as green manure to improve soil nutrition. Both blue-green algae and Azolla are important for agriculture - blue-green algae fix nitrogen while Azolla acts as a biofertilizer when used in rice fields and on other crops.
This document discusses induced systemic resistance (ISR) in plants. It provides historical context on studies of induced resistance dating back to the late 1800s. ISR is defined as a phenomenon where treatment with certain chemicals or pathogens activates a plant's defenses throughout the plant. Key findings include:
- ISR is activated by rhizobacteria and involves jasmonic acid and ethylene signaling rather than salicylic acid signaling as in systemic acquired resistance.
- Several bacteria, fungi, chemicals, and elicitors are reported to induce ISR through different signaling pathways and defense responses.
- Further research is needed to fully understand ISR signaling and apply it effectively in fields to control plant diseases.
This document defines fungicides and related terms like fungistat and antisporulant. It classifies fungicides based on their mode of action (protectant, therapeutant, eradicatcant), general use (seed protectants, soil fungicides, foliage fungicides), and chemical composition (sulphur fungicides, copper fungicides, dithiocarbamates). Specific examples are provided for each classification along with the diseases they control.
“Any living organism supplying plant nutrients directly or indirectly is regarded as biofertilizer. They are not synthetically manufactured in factory.”
introduction to mycorrhizae and its role in P uptakeFari Rajput
This document discusses mycorrhiza and its role in phosphorus uptake by plants. It begins by defining mycorrhiza as a symbiotic relationship between plant roots and fungi, where the fungi help facilitate nutrient and water uptake in exchange for carbohydrates from the plant. There are two main types of mycorrhiza - ectomycorrhiza and endomycorrhiza. Mycorrhizal fungi take up nutrients from soil through a mycorrhizal pathway and transfer them to the plant, helping the plant access nutrients like phosphorus that are poorly mobile in soil. They do this through mechanisms like producing phosphatases and organic acids to solubilize inorganic and organic phosphorus sources, and storing
This document discusses different types of mycorrhizal associations between fungi and plant roots. The main types discussed are ectomycorrhizae, arbuscular mycorrhizae, orchid mycorrhizae, ericoid mycorrhizae, arbutoid mycorrhizae, and monotropoid mycorrhizae. These associations are typically mutualistic, with fungi helping plants uptake nutrients and water, and plants providing fungi with carbohydrates. Ectomycorrhizal fungi form sheaths around roots and penetrate the cortex, while arbuscular mycorrhizal fungi form structures called arbuscules and vesicles within root cells.
The document discusses biofungicides, which are biologically active substances derived from organisms that prevent and treat plant diseases. There are three main types of biofungicides: agricultural antibiotics produced through microbial fermentation, bacteria-based fungicides like Bacillus subtilis that inhibit fungi and promote growth, and fungal fungicides including Trichoderma species that control various plant pathogens. Biofungicides have advantages over chemical fungicides in that their active ingredients are naturally occurring microorganisms that are less toxic and help improve soil health.
Mechanism of disease control by endophytesPooja Bhatt
The document discusses alternative methods for pest management to address problems with chemical pesticides such as development of resistance and environmental contamination. It suggests that biological control using endophytic microorganisms is a promising alternative as endophytes have antagonistic properties against plant pathogens. Endophytes can inhibit pathogens through direct mechanisms such as hyperparasitism, competition, antibiosis, and lytic enzyme production or indirect induction of host plant resistance. Case studies provide examples of endophytes inhibiting fungal plant pathogens through siderophore production, parasitic growth, and antibiotic compounds.
The document discusses mycorrhizae and their potential as biocontrol agents. It describes the evolution and discovery of mycorrhizal fungi. There are different types of mycorrhizal associations based on the relationship with host plants. The document discusses the distribution of mycorrhizal fungi among host and non-host plants. It explains how vesicular arbuscular mycorrhizae (VAM) can act as biocontrol agents by suppressing soil-borne pathogens through various mechanisms like physiological alterations in host plants, competition for space and nutrients, and changes in root exudates. The mycorrhizal intensity was found to be higher in healthy plants compared to diseased plants in fields studied
This document discusses biosolubilization of insoluble phosphates in a fluidized bed bioreactor using immobilized microorganisms. Phosphate solubilizing bacteria and fungi were entrapped in calcium alginate and polyacrylamide gels and used to solubilize mineral phosphates in a fluidized bed bioreactor. This allows for reusability of the biocatalyst and control of reactions without contamination. The longevity of solubilization activity was tested through repeated batch experiments. Bioconversion using these immobilized microorganisms occurs at low temperatures and provides a sustainable way to extract and solubilize phosphates compared to conventional processes.
The document discusses systemic acquired resistance (SAR), which confers long-lasting protection against a broad spectrum of pathogens. SAR is induced by initial infection and involves the signaling molecule salicylic acid, leading to accumulation of pathogenesis-related proteins throughout the plant. Key regulators of SAR include NPR1, which is required for SAR, and salicylic acid, which is involved in transmitting the defense signal systemically.
phyllosphere is a dynamic rapidly changing area surrounding the germinating seed. there are two categories of microbes one is positively enhancing and negatively reducing the plant yield
This document discusses Azotobacter, a genus of nitrogen-fixing bacteria that can be used as a biofertilizer. It describes the key species of Azotobacter, their identifying characteristics, and their benefits to agriculture. Azotobacter promotes plant growth by fixing atmospheric nitrogen and producing plant hormones. It also functions as a biocontrol agent by suppressing plant pathogens. The document outlines Azotobacter's mode of action in plants and provides examples of increased crop yields and quality from its use as an inoculant. It also discusses the maintenance, selection, and mass production methods for Azotobacter cultures.
This document discusses phosphorus uptake by mycorrhizal fungi. It outlines two pathways for phosphorus uptake - the direct pathway where plants absorb phosphorus directly from the soil, and the mycorrhizal pathway where a symbiotic relationship with mycorrhizal fungi allows for greater phosphorus absorption. The mycorrhizal pathway involves mycorrhizal fungi extending their hyphae into the soil to absorb phosphorus, which is then transported to the plant. Mycorrhizal associations are significant as they improve plant nutrition and phosphorus solubility in soil.
The rhizosphere is the region of soil surrounding plant roots that is influenced by root secretions like mucilage, exudates, and lysates. It contains many microorganisms in complex relationships with the plant roots. Root secretions, collectively known as rhizodeposition, enrich the soil environment and stimulate microbial growth in the rhizosphere compared to bulk soil, as measured by the R:S ratio of microorganisms. Rhizodeposition includes a variety of organic compounds that influence soil nutrients and microbes.
Effect of Clariodeoglomusclariodeorum on morphology and abundant of carrot ro...AI Publications
The roothairs are important components of the root for absorbing nutrients for plants and also secreting the plant-produced secretes. Morphology and their number are influenced by various environmental and internal factors and are regulated by them. Mycorrhizal fungi are established through the root and their presence in the root can affect root’s physical and chemical properties.The aim of this study was to evaluate the effect of the presence of the fungus in the roots on the characteristics of the capillary roots.In order to remove the effect of other microorganisms on the results of the experiment, this experiment was performed in vitro on the roots of the carrot secondary phloem tissue culture. In this experiment, the fungus could affect the characteristics of the root hairs: their number (22% decrease in the root hairs number in the root hair area of the root) and their length (A decrease of 21.3% in the length of capillary roots in mycorrhizal plants). These changes in the characteristics of capillary roots were also caused by the presence of fungal structures in the roots as well as by the decrease in the production of strigolactones. In this study, changes in the production of strigolactones calculated by using their effect on seed germination of Phelipancheaegyptiaca.
Plants require essential nutrients for their growth and development that are mainly acquired from soil by their roots. Nutrient stress is an environmental condition that can seriously affect the production and quality of crop produce. Biofertilizers are the organisms (Bacteria, fungi, cyanobacteria, etc.) that enrich the nutrient quality of soil. Plants have a number of beneficial relationship with such organisms. Among these AM-Fungi are ubiquitous and form a mutuality relationship with roots of most plant species.
Functional Genomics of Plant Pathogen interactions in Wheat Rust PathosystemSenthil Natesan
Cereal rust fungi are pathogens of major importance to agriculture, threatening cereal production worldwide. Targeted breeding for resistance, based on information from fungal surveys and population structure analyses of virulence, has been effective. Nevertheless, breakdown of resistance occurs frequently and continued efforts are needed to understand how these fungi overcome resistance and to determine the range of available resistance genes. The development of genomic resources for these fungi and their comparison has released a torrent of new ideas and approaches to use this information to assist pathologists and agriculture in general. The sequencing of gene transcripts and the analysis of proteins from haustoria has yielded candidate virulence factors among which could be defence-triggering avirulence genes. Genome-wide computational analyses, including genetic mapping and transcript analyses by RNA sequencing of many fungal isolates, will predict many more candidates (Bakkeren et al., 2012)
Dissecting the mechanisms of host-pathogen systems like wheat-rust, including pathogen counter-defenses will ensure a step ahead towards understanding current outcomes of interactions from a co-evolutionary point of view, and eventually move a step forward in building more durable strategies for management of diseases caused by fungi (Hadrami et al.,2012)
Defensive mechanisms in Plants: The role of component plant cells in defense ...Agriculture Journal IJOEAR
— Plants are often exposed to various environmental stresses such as extreme temperatures, drought, and disease and pest attack. In natural systems, plants face a plethora of antagonists and thus posses a myriad of defense and have evolved multiple defense mechanisms by which they are able to cope with various kinds of biotic and abiotic stresses. In fact plants defense against stresses by different ways. The role of cellular organelles is very important in this way. Cell wall and their derivatives such as oligosaccharins as biochemical defenser or for example trichomes as mechanical defenser is the frontline of the plant defense system. Also Plants have evolved a multi-layered immune system that dynamically responds to pathogens alike cell membrane that is a key mediator of communication between plants and microbes. Cytoplasm and the membrane-bounded structures (organelles) defense against different kind of stresses. The role of cellular organelles in plant defense relate to their enzymes primarily. Enzymes such as proteases, esterases and ribonucleases in cytoplasm, PM H+-ATPases in plasma membrane or β glucosidases included cyanogenic glucosides, saponins, glucosinolates or DIMBOA (2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one) glucoside in ER are responsible for plant defense. Also ROSs plus SA and JA in chloroplast and mitochondria play an important role in immune plant system. In nucleus macromolecules including nucleoporins, importins, and Ran-GTP-related components, are essential to mount an efficient immune response in response to different pathogens. And in Golgi apparatus, peroxysomes and vacuoles, glycosyltransferases, myrosinase and hydrolytic enzymes are liable for plant defense respectively. Keywords— biotic and abiotic stresses; organells; plant defense.
Ecto and endomycorrhizae and their significanceRitaSomPaul
A part of Botany (Hons) syllabus in Mycopathology illustrates the basic differnces in ectomycorrhizae and endomycorrhizae as well as their significance
A SEMINAR REPORT ON POLLEN MICROBES BY TEMIDAYO FARORK OLAPADE.
Microorganisms including fungi, bacteria, and viruses live in flowers and are thought to affect pollination. Microbial influence the effectiveness of pollinator visits is poorly understood and depends on the context. The effect of microbes on pollen performance is underappreciated. Beyond the effect of pathogenic viruses, the impacts of pollen-transmitted endophytic microbes on pollen viability or tube growth are unknown but could affect the outcome of pollen receipt. Future research integrating microbes into pollination should broaden taxonomic diversity of microbes, pollinators and plants and the processes under study. Crops aimed at feeding an exponentially growing population are often exposed to a variety of harsh environmental factors. Although plants have evolved ways of adjusting their metabolism and some have also been engineered to tolerate stressful environments, there is still a shortage of food supply. An alternative approach is to explore the possibility of using rhizosphere microorganisms in the mitigation of abiotic stress and hopefully improve food production. Several studies have shown that rhizobacteria and mycorrhizae organisms can help improve stress tolerance by enhancing plant growth; stimulating the production of phytohormones, siderophores, and solubilizing phosphates; lowering ethylene levels; and upregulating the expression of dehydration response and antioxidant genes.
This document describes a study that evaluated seven Bacillus plant growth-promoting rhizobacteria (PGPR) strains for their ability to promote plant growth and induce systemic resistance against bacterial leaf blight in rice caused by Xanthomonas oryzae pv. oryzae. The PGPR strains were tested as fresh suspensions and powder formulations applied to rice seeds. Seed treatments with fresh suspensions of strains B. subtilis GBO3 and B. pumilus SE34 resulted in the highest germination rates and seedling vigor. These strains also induced the strongest systemic resistance in rice plants against X. oryzae pv. oryzae, reducing disease severity by over 50% compared to untreated controls
Fungi in Agriculture and Forestry: A Boon to Human Welfare by Dr. Pampi GhoshPampi Ghosh
One day National webinar on " Fungi in human welfare".
Invited Talk
Speaker 2: Dr. Pampi Ghosh
Assistant Prof (SSS), Dept. of Botany, SBM, Kapgari, Jhargram, W.B.
You tube link: Presentation of P. Ghosh: https://youtu.be/2jTXnxv3WiY
on 05/01/2022 (12:30 to 1:20 p.m)
Thank to the convenor of this webinar committee Dr. Chhya K. Bhalsankar, HOD, Botany Dept. , AJMVP's New Arts, Commerce and Science college Shevgaon, Ahmednagar
vice Principal Sir
Dr. YS sudake, AJMVP's New Arts, Commerce and Science college Shevgaon, Ahmednagar
and
Principal Sir Dr. PR Kunde, AJMVP's New Arts, Commerce and Science college Shevgaon, Ahmednagar, M.S.
This study investigated the role of flavonoids in promoting the germination of spores of the arbuscular mycorrhizal fungus Rhizophagus irregularis and colonization of tomato roots. In vitro and greenhouse experiments tested various subclasses of flavonoids at physiological doses. The flavone chrysin and flavonols quercetin and rutin were found to stimulate spore germination and root colonization, confirming a role for flavonoids as pre-symbiotic signaling molecules in the AM symbiosis. The results suggest flavonoids could be used in AM fungal inoculants to improve symbiosis establishment and inoculant efficiency for more sustainable agriculture.
Defense Mechanism in Plants Against InsectsJayantyadav94
Plants and insects living together for more than 350 million years
Evolutionary between plants and insects resulted in the development of defence system in plants that has the ability to recognize signals from damaged cells
Activates the plant immune response against the insects
Plants have the ability to distinguish between herbivory and mechanical damage, such as hail and wind, as well as to recognize oviposition.
This feature is needed to avoid wasting expensive defence resources, since production and release of defence responses only benefits herbivore challenged plants.
This document describes a microarray study that analyzed gene expression changes in Nicotiana benthamiana in response to infection by South African cassava mosaic virus (SACMV). The microarray identified over 600 genes whose expression was altered at 21 days post infection, including genes involved in transcription, defense responses, and hormone signaling. Notable changes were seen in genes related to sucrose and starch metabolism, as well as genes associated with intracellular movement and the cytoskeleton. The results provide insight into the complex molecular interactions between SACMV and its host plant during the infection process.
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2. The pros discussed include the broad spectrum antimicrobial activity of silver nanoparticles, their ability to be synthesized through green methods, effectiveness at low concentrations, and reduced likelihood of microbial resistance developing.
3. The cons examined are the potential harmful effects on beneficial microbes, environmental impacts, toxicity risks to plants, development of microbial resistance over time, and human health concerns. The presentation concludes by emphasizing the need for further research and regulatory guidelines to ensure the safe use of silver nanoparticles in agriculture.
Evaluating Kenyan Dolichos (Lablab purpureus L.) Genotypes for Resistance to ...Premier Publishers
The objective of this study was to evaluate eighteen Dolichos lablab genotypes for resistance to Maruca vitrata and Helicoverpa armigera in the field using morphological markers. The study design was Randomized Complete Block (RCBD) with separation of mean done using Turkey’s range of test. Eldoret, KALRO Njoro and KALRO Kakamega were the study sites. Morphological parameters of pods were studied to determine whether they have any influence on resistance of Dolichos lablab to M. vitrata and H. armigera. The pod damage (%) of each genotype was calculated and given a resistance rating of 1-5 score damage. Genotype G2, Bahati and W7 were resistant to M. vitrata in a scale of 1(0-10%; low infestation), Bahati and W7 were moderately resistant to H. armigera in a scale of 2(11-30%; moderate infestation). Genotype LG1MoiP10 was susceptible to M. vitrata in a scale of 4 (51-70%; severe infestation) and genotype M5 was intermediate to H. armigera in a scale of 3 (31-50%; high infestation). There was positive significant correlation in H. armigera and M. vitrata pod damage with days to maturity, growth habit, and pod attachment. Pod length and pod fragrance were positively correlated to M. vitrata. Negative correlation was detected in pod thickness, pod pubescence and raceme position to pod damage by H. armigera and M. vitrata. The study identified G2, Bahati and W7 as promising resistant genotypes and can be used in Dolichos breeding program. However, there is need to further evaluate them in different environments and seasons for reliability.
Endophytic microbes live within plant tissues without causing harm and can benefit plants through various mechanisms. This document discusses endophytic bacteria and fungi, their transmission within plants, and how they can promote plant growth, act as biocontrol agents, and increase stress tolerance in plants. Specifically, endophytes produce plant hormones, fix nitrogen, make nutrients more available, and induce systemic resistance to pathogens or tolerance to stresses like drought. Their interactions with plants demonstrate potential for agriculture and phytoremediation.
what is Antixenosis, Antibiosis, and Tolerance.pptxRamshaShaikh11
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Content:
Introduction
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Historical perspectives
Advantages and Disadvantages of HPR
Mechanisms of Resistance
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Behavior in Relation to Host Plant Factor
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Avs role of plant growth promoting rhizobacteria in diseaseAMOL SHITOLE
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How to Manage Your Lost Opportunities in Odoo 17 CRMCeline George
Odoo 17 CRM allows us to track why we lose sales opportunities with "Lost Reasons." This helps analyze our sales process and identify areas for improvement. Here's how to configure lost reasons in Odoo 17 CRM
ISO/IEC 27001, ISO/IEC 42001, and GDPR: Best Practices for Implementation and...PECB
Denis is a dynamic and results-driven Chief Information Officer (CIO) with a distinguished career spanning information systems analysis and technical project management. With a proven track record of spearheading the design and delivery of cutting-edge Information Management solutions, he has consistently elevated business operations, streamlined reporting functions, and maximized process efficiency.
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Date: May 29, 2024
Tags: Information Security, ISO/IEC 27001, ISO/IEC 42001, Artificial Intelligence, GDPR
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Chapter wise All Notes of First year Basic Civil Engineering.pptxDenish Jangid
Chapter wise All Notes of First year Basic Civil Engineering
Syllabus
Chapter-1
Introduction to objective, scope and outcome the subject
Chapter 2
Introduction: Scope and Specialization of Civil Engineering, Role of civil Engineer in Society, Impact of infrastructural development on economy of country.
Chapter 3
Surveying: Object Principles & Types of Surveying; Site Plans, Plans & Maps; Scales & Unit of different Measurements.
Linear Measurements: Instruments used. Linear Measurement by Tape, Ranging out Survey Lines and overcoming Obstructions; Measurements on sloping ground; Tape corrections, conventional symbols. Angular Measurements: Instruments used; Introduction to Compass Surveying, Bearings and Longitude & Latitude of a Line, Introduction to total station.
Levelling: Instrument used Object of levelling, Methods of levelling in brief, and Contour maps.
Chapter 4
Buildings: Selection of site for Buildings, Layout of Building Plan, Types of buildings, Plinth area, carpet area, floor space index, Introduction to building byelaws, concept of sun light & ventilation. Components of Buildings & their functions, Basic concept of R.C.C., Introduction to types of foundation
Chapter 5
Transportation: Introduction to Transportation Engineering; Traffic and Road Safety: Types and Characteristics of Various Modes of Transportation; Various Road Traffic Signs, Causes of Accidents and Road Safety Measures.
Chapter 6
Environmental Engineering: Environmental Pollution, Environmental Acts and Regulations, Functional Concepts of Ecology, Basics of Species, Biodiversity, Ecosystem, Hydrological Cycle; Chemical Cycles: Carbon, Nitrogen & Phosphorus; Energy Flow in Ecosystems.
Water Pollution: Water Quality standards, Introduction to Treatment & Disposal of Waste Water. Reuse and Saving of Water, Rain Water Harvesting. Solid Waste Management: Classification of Solid Waste, Collection, Transportation and Disposal of Solid. Recycling of Solid Waste: Energy Recovery, Sanitary Landfill, On-Site Sanitation. Air & Noise Pollution: Primary and Secondary air pollutants, Harmful effects of Air Pollution, Control of Air Pollution. . Noise Pollution Harmful Effects of noise pollution, control of noise pollution, Global warming & Climate Change, Ozone depletion, Greenhouse effect
Text Books:
1. Palancharmy, Basic Civil Engineering, McGraw Hill publishers.
2. Satheesh Gopi, Basic Civil Engineering, Pearson Publishers.
3. Ketki Rangwala Dalal, Essentials of Civil Engineering, Charotar Publishing House.
4. BCP, Surveying volume 1
it describes the bony anatomy including the femoral head , acetabulum, labrum . also discusses the capsule , ligaments . muscle that act on the hip joint and the range of motion are outlined. factors affecting hip joint stability and weight transmission through the joint are summarized.
Walmart Business+ and Spark Good for Nonprofits.pdfTechSoup
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The webinar may also give some examples on how nonprofits can best leverage Walmart Business+.
The event will cover the following::
Walmart Business + (https://business.walmart.com/plus) is a new shopping experience for nonprofits, schools, and local business customers that connects an exclusive online shopping experience to stores. Benefits include free delivery and shipping, a 'Spend Analytics” feature, special discounts, deals and tax-exempt shopping.
Special TechSoup offer for a free 180 days membership, and up to $150 in discounts on eligible orders.
Spark Good (walmart.com/sparkgood) is a charitable platform that enables nonprofits to receive donations directly from customers and associates.
Answers about how you can do more with Walmart!"
This document provides an overview of wound healing, its functions, stages, mechanisms, factors affecting it, and complications.
A wound is a break in the integrity of the skin or tissues, which may be associated with disruption of the structure and function.
Healing is the body’s response to injury in an attempt to restore normal structure and functions.
Healing can occur in two ways: Regeneration and Repair
There are 4 phases of wound healing: hemostasis, inflammation, proliferation, and remodeling. This document also describes the mechanism of wound healing. Factors that affect healing include infection, uncontrolled diabetes, poor nutrition, age, anemia, the presence of foreign bodies, etc.
Complications of wound healing like infection, hyperpigmentation of scar, contractures, and keloid formation.
Mycorrhizal induced resistance and plant innate immunity
1. Mycorrhizal induced resistance and
plant innate immunity
Presented by-
Nongthombam Olivia Devi
PhD 1st Year 2nd sem
CAU/CPGS/PATHO/P16/03
School of Crop Protection, Plant Pathology
CPGS, Umiam ,Barapani
CREDIT SEMINAR
ON
2. CONTENT
Introduction
Types of mycorrhiza
MIR phases
Mycorrhizosphere effect
Strigolactones: multipurpose rhizosphere signals
Modulation of host immunity
Signal transduction between AMF and plant upon pathogen
attack
Priming of JA-dependent Defenses in Mycorrhiza- Induced
Resistance
Mycorrhiza Interactions with phytopathogens
Case Studies
3. INTRODUCTION
Mycorrhizas are mutualistic associations
between the roots of 80 % of terrestrial plant
species and fungi (Schüßler et al., 2001).
Greek “mycos” and “rhiza” meaning “fungus-
root,” oldest and most widespread plant
symbiosis.
Plants develop enhanced defensive capacity due
to infection by arbuscular mycorrhizal fungi
(AMF) (Cameron et al.2013).
‘Mycorrhiza-induced resistance’ (MIR) provides
systemic protection against a wide range of
attackers.
Coined the term
mycorrhiza (1885)
4. The mycorrhizal fungi germinate make way to root.
Roots colonized by fungi, penetrate root create internal
fungal network structure, exchange nutrients and sugar.
Hyphae develop outside roots to explore soil.
Nutrients
Sugar
How does symbiosis take
place?
P,N,K
6. • The fungal structure entirely within the host root, common with
herbaceous plants also associated with some woody plants.
• Associations where Glomeromycete
fungi produce arbuscules, hyphae, and
vesicles within root cortex cells.
• Defined by the presence of arbuscules.
• Obligate biotrophs, endotrophic.
• Act as bioprotectors of plants(Xavier
and Boyetchko, 2004)
ENDOMYCORRHIZA
ARBUSCULAR
ENDOMYCORRHIZA
Fig 2.Arbuscule of a Glomus species
in a root cortex cell
Fig 1:Vesicles of a Glomus species in
a root cortex.
(Fig. Source-Brunett,2008)
7. Most advanced symbiotic
association.
Basidiomycota and include
common woodland
mushrooms, such
as Amanita spp., Boletus spp.
and Tricholoma spp(David et
al. 2011).
Form a mantle around roots
and a Hartig net between root
cells (Brunett,2008).
ECTOMYCORRHIZAE
Fig 3-Suillus sibiricus ectomycorrhizae on
inoculated Pinus albicaulis seedling in the
nursery
Fig 4-Cortical hartig net of ECM root
(Fig. Source-Brunett,2008)
8. Ericoid Arbutoid Monotropoid Orchidaceous
Coils of hyphae
within very thin
roots(root hairs) of
the ericaceae.
Found in certain
plants in the
Arbutoideae and
Pyrolaceae
characterized by
hyphal coils in
epidermal cells.
Mycorrhizal
association
formed by the
achlorophyllous
plants of the
Montropaceae.
Association where
coils of hyphae
penetrate within
cells in a root or
stem in the plant
family
orchidaceae.
Fig 6-Arbutus
unedo root with Hartig
net (arrows), coils (C)
and mantle (M) of
hyphae.
Fig 8-Orchid
mycorrhizas with
hyphae in trichomes
and hyphal coils in stem
of Pterostylis vittata
Fig 7-Monotropa root
with epidermal Hartig
net (H) and mantle
(M) in a cross-section
viewed with UV light.
Fig 5- Ericoid
mycorrhizas with hyphal
coils in hair roots
of Leucopogon
verticillatus
9. Fig 9-The principle structural features of the five main types of mycorrhiza.
(David et al. 2011)
10. SOME OF THE COMMERCIALLY IMPORTANT PLANT GROUPS THAT BENEFIT FROM -
MYCORRHIZAL FUNGI:
11. What is induced resistance and innate
immunity?
INDUCED RESISTANCE - enhanced
defensive capacity of plant when
appropriately stimulated.
Systemic Acquired Resistance (SAR)
Induced Systemic Resistance (ISR)
Plant defenses preconditioned by
prior infection results in resistance
against subsequent challenge by a
pathogen (Choudhary et al. 2007).
12. Plants are invaded by an array of pathogens of which only a
few succeed in causing disease. The attack by others is
countered by a sophisticated immune system possessed by
the plants (Muthamilarasan and Prasad, 2003).
The plant immune system is broadly divided into two-
Microbial-associated molecular-patterns-triggered
immunity (MTI)
Effector-triggered immunity (ETI).
INNATE IMMUNITY OF PLANTS
16. Mycorrhizosphere effect
Mycorrhizal hyphae exude chemical
compounds , have selective effect on
the microbial communities.
(mycorrhizosphere) (Duponnois et
al. 2008).
Enhanced microbial activity
surrounding mycorrhizal roots
(mycorrhizosphere effect.)
Mycorrhizosphere-inhabiting bacteria,
called ‘ mycorrhiza helper bacteria’
(MHB), stimulate mycorrhizal
symbioses ,deliver ISR-eliciting
signals. (Duncan et al.2014).
Multitrophic interactions.
Fig source-Audet,2012
Fig 11-Defining the
mycorrhizosphere and its
zone of influence
18. Fig 13-Schematic summary of the mechanisms by which the endobacterium affects
G. margarita metabolism Source-Salvioli et al.201
19. Strigolactones: multipurpose rhizosphere signals
• Early phase of colonization
strigolactone (SL) production high.
• Later AMF perceived as an alien
organism,salicylic acid (SA) levels
increased.
• well-established mycorrhiza both SL
and SA production repressed while
biosynthesis of jasmonates (JA)
increases.
• Plant sesquiterpenes that are exuded from roots.
• Role for seed germination of parasitic weeds
(Orobanche and Striga) (Garrido et al.2009).
• Stimulates branching of hyphae during germination of the spores
of AM fungi(Besserer et al.2006).
Fig. 14 Model for hormonal changes in the roots
associated to thearbuscular mycorrhizal symbiosis
Source-Jung et al. (2012). J.Chem Ecol
20. Strigolactones Stimulate Arbuscular Mycorrhizal Fungi by
Activating Mitochondria
• Branching Factor of a monocotyledonous
plant Sorghum strigolactone.
• Strigolactones strongly and rapidly
stimulated cell proliferation of Gigaspora
rosea at conc. 10-13 M.
• Within 1 h treatment, density of
mitochondria in fungal cells increased, their
shape and movement changed .
• Strigolactones stimulated spore
germination Glomus intraradices and Gl.
claroideum.
Besserer et al., 2006, PLoS Biol
Fig15
23. The plant immune system responding to AMF infection
..
AMF initiate
infection of the
root cortex
Microbe-
associated
molecular
patterns
(MAMPs) from
the fungus
recognised by
the plant innate
immune
system
transient
expression of
MAMP-
triggered
immunity (red
cells) and
generation of
long-distance
signals in the
vascular
tissues
induce long-
lasting priming
of salicylic acid
(SA)-
dependent
defences and
systemic
acquired
resistance
(SAR).
Long distance signal
MAMP
triggered
immunuty
24. Fig. 17 Model for the modulation of host immunity in the ecto- and
endomycorrhizal symbioses
B
Source-Zamioudis et al .(2012) The American Phytopathological
Society
25. Symbiotic mycorrhizal
fungi reduce stimulation
of the host’s immune
system.
Mycorrhizal fungi initially
elicit an MTI response,
which is subsequently
suppressed
Insights into the genome
of mycorrhizal fungi:
secretion of effector-like
molecules.
Mycorrhizal fungi
suppress SA-mediated
defense responses by
utilizing the Myc
signaling pathway
MODULATION OF HOST IMMUNITY
26. Modulation of plant defense response
Mycorhiza
establish
ment
Change in
Jasmonic
acid
Change in
Salicylic
acid
Change in
Ehtylene
Stimulate
abscisic
acid
(ABA)
Transported
through the
xylem to the
shoot, prime cell
wall defences.
combined impact of plant immune
modulation, enhanced sugar allocation,
increased nutrient uptake, and fungal
modification of root exudates leads to
changes in root exudation chemistry and
selection of specific mycorrhizosphere
bacteria
27. Fig 18-Hypothetical model representating the regulatory mechanism involved in
plant defense response during establishment of AM fungi
Garrido et al. (2002). Journal of experimental biology
28. Signal transduction between AMF and plant upon pathogen attack
Fig 19- Schematic representation of AMF-induced defence signaling in plant cell
Defense gene induction
Defense related proteins
Ellicitors inducing defense related genes
Hypersensitivity
Ca
JA
Biosynthesis
Source-Khan et al., 2010,J.Phytol.
29. Priming of JA-dependent Defenses in Mycorrhiza- Induced
Resistance
• In non-mycorrhizal plants (−
AMF) the initial wound signal
activates the JA-dependent
pathway that leads to the
activation of defense genes (DG).
• In mycorrhizal plants (+ AMF) the
response to the wound signal is
amplified leading to a primed
defense response.
Fig. 3 Priming of jasmonate-dependent
wound signaling in the shoots.
“This priming is common upon interaction
with beneficial microorganisms, and has
important fitness benefits compared to direct
activation of defenses’’
31. Table 2 -Defense mechanisms associated with ISR by AMF
Source-(Cameron et al.2013).
32. Table 3. List of genes induced after AMF colonization in host plant and are responsible for
the plant’s defense against phytopathogen
Sl.No
.
Genes Products Function Source Reference
1 TC104515 Cysteine rich
protein
Antifungal property M. truncatula Liu et al.,
2007
2. TC101060 Cysteine rich
protein
Antifungal property M. truncatula Liu et al.,
2007
3 PR-1a PR-1a protein Pathogenesis-related
(Antimicrobial
Tomato Conrath et
al., 2006
4 BLG β-1,3 Glucanase
(PR protein family)
Antifungal property Tomato tomato Conrath et
al., 2006
5 VCH3 Chitinase protein
(PR protein family)
Antifungal property against
Meloidogyne incognita
Vitis
amurensis
Rupr.
(Grapevine)
Li et al., 2006
6. Pal Phenylalanine
ammonia lyase
(PAL)
enzyme
leads to production of
phytoalexins and phenolic
substances
Rice Blilou et al.,
2000b.
7. Ltp Lipid transfer
protein
Antimicrobial Rice Blilou et al.,
2000b.
Source-khan et al.2010
33. (Source-Trouvelot et al.2015, Agron. Sustain. Dev)
Increase plant resistance against
biotic stresses while reducing
phytochemical inputs
Increase plant/soil adherence
Improve soil water retention
Improve soil structure and
stability
Increase plant resistance to abiotic
stresses (Drought,salinity,metals
and mineral nutrient depletion)
Promote plant growth
Bioregulation of plant development
and increase in plant quality for
human health
Reduce fertilizer requirement
34. Mycorrhiza Interactions with phytopathogens
• Mycorrhiza fungi make roots more resistant to
invasion by pathogen like Fusarium, Rhizoctonia,
Macrophomina, or Verticillium, bacteria such as
Erwinia carotovora; or oomycetes like
Phytophthora, Pythium, and Aphanomyces and
soil inhabiting nematodes (Whips, 2004).
• AMF have impact on root-feeding insects, mostly
members of the genus Otiorhynchus, or weevils
(Koricheva et al., 2009).
35. Pathogen Host Effect in Mycorrhizal
plants
Olpidium brassicae Tobacco,lettuce Reduction of infection
Pythium ultimum soybean none
Pythium ultimum poinsettia Reduced stunting
Phytophthora megasperma soybean Fewer plants killed
P.palmivora papaya none
P.parasitica Citrus Reduction of damage
Rhizoctonia solani poinsettia Reduction of damage
Thielaviopsis basicola Tobacco,alfalfa,cotton Less stunting,inhibition of
chlamydospore
Cylindrocarpon destructans Strawberry Less stunting,reduction of
infection
Cylindrocladium scoparium Yellow poplar Less stunting,reduction of
infection
Fusarium oxysporium tomato Less stunting,reduction of
infection
Fusarium oxysprium cucumber Less stunting,reduction of
infection
Phoma terrestris onion Less stunting,reduction of
infection
Table 4 -Effect of AM on soil borne diseases caused by Fungi
Source-Declerck et al, (2010) in vitro culture of mycorrhizae,pp-131
36. Bacterial species AMF species Interaction type Reference
Azospirillum
brasilence
Glomus intraradices Neutral Hildebrandt et al
.(2002)
Bacillus
chitinosporus,B.pabuli
and other spore-
associated bacteria
G.clarum Positive,neutral or
negative
Xavier and
Germida(2003)
Clavibacter
michiganensis
ssp.michiganensis
G.intraradices Neutral Filion et al.(1999)
Corynebacterium sp. G.versiforme Positive Mayo et al. (1986)
Escherichia coli G.inraradices neutral Hildebrandt et al(2002)
Paenibacillus validus G.intraradices positive Hildebrandt et al(2002)
Pseudomonas sp. Endogone sp. positive Mosse(1962)
P.aeruginosa G.intraradices positive Villegas and
Fortin(2001,2002)
P.fluorescens Gigaspora margarita positive Bianciotto et al.(1996)
P.putida G.intraradices Positive or neutral Villegas and
Frotin(2001,2002)
Table 5-INTERACTION BETWEEN AM FUNGI AND BACTERIA IN VITRO
Source-Declerck et al, (2010) in vitro culture of mycorrhizae,pp-219
37. CROP NEMATODES FUNGUS EFFECT REFERENCE
Pepper R.Similis and
M.incognita
Glomus mosseae 60% population
reduction
Sivasprasad and
Sheela,1998
Cardamon M.incognita G.fasciculatum Nematode population
reduction
Sivaprasad et
al.,2001
Ginger M.incognita G.fasiculatum Nematode population
reduction
Joseph et al.,2001
Brinjal M.incogita G.fasiculatum Nematode population
reduction
Trivedi,2003
Coconut R.similis Glomus sp. Reduced lesion
no.and improved
plant gowth
Koshy et al,1998
Banana M.Incognita and
R.similis
G.mosseae Nematode population
reduction and
improved plant
growth
Trivedi,2003
Tomato M.incognita G.fasiculatum Smaller galls and less
no.of giant
cells.Reduction in
no.of galls,egg
masses,eggs and
juvenile
Suresh et al,1985 and
Sharma et al.,1994
Table 6-AMF INTERACTION WITH NEMATODES
Source-Nehru (2005),Plant disease biocontrol management,pp-199-200
38. MYCORRHIZA AND VIRUS INTERACTION
THE GENERAL RESPONSE OF MYCORRHIZAL PLANTS TO THE
PRESENCE OF VIRAL PATHOGENS IS AS FOLLOWS-
Mycorrhizal plants apparently enhanced the
rate of multiplication of viruses in some plants
More leaf lesions were found on mycorrhizal
plants than on non mycorrhizal plants.
The number of AMF spores in the
rhizosphere was reduced considerably
Source-Xavier and Boyetchko (2004),Fungal biotech.in Agril.,Food and
Environmental appl.
39. Systemic Resistance in Arabidopsis Conferred by the Mycorrhizal
Fungus Piriformospora indica Requires Jasmonic Acid Signaling and
the Cytoplasmic Function of NPR1
• Analyzed specific defense pathways for powdery mildew
(Golovinomyces orontii) resistance induced by Piriformospora
indica in Arabidopsis.
• Piriformospora indica root colonization reduced G. orontii conidia in
wild-type (Col-0), npr1-3 (non expressor of PR genes 1-3) and NahG
plants, but not in the npr1-1 null mutant.
• Two jasmonate signaling mutants non-responsive to P. indica, and
jasmonic acid-responsive vegetative storage protein expression
primed and elevated in response to powdery mildew.
Source-Stein et al.2008, Plant
cell Physiol
40. Enhanced tomato disease resistance primed
by arbuscular mycorrhizal fungus
• Tomato plants (S. lycopersicum ) inoculated with mycorrhizal
fungus Funneliformis mosseae and A. solani (ACCC36110) tomato
early blight disease.
• AMF pre-inoculation increases activities of β-1,3-glucanase,
chitinase, phenylalanine ammonia-lyase (PAL) and lipoxygenase
(LOX) in tomato leaves upon pathogen inoculation.
• provoked defense responses of three genes encoding
pathogenesis-related proteins, PR1, PR2, and PR3, as well as
defense-related genes LOX, AOC, and PAL, in tomato leaves.
Source-Song et al. (2015) Front. Plant Sci.,
41. TABLE 7. Mycorrhizal colonization rates, disease incidences, and indices of tomato
plants inoculated with either Funneliformis mosseae, Alternaria solani, or both.
FIGURE 21. Disease symptoms of early blight in leaves of tomato plants
with or without mycorrhizal colonization by Funneliformis mosseae.
Source-Song et al. (2015) Front. Plant Sci.,
42. Induction of defense responses in common bean
plants by arbuscular mycorrhizal fungi
• Interaction between AM fungi and Rhizoctonia ( root rot
disease of common bean) investigated in pot experiment.
• Mixture of Egyptian formulated AM (Multi- VAM) suspension
form (1×106 unit L−1 in concentration) used at dilution of 5ml
L−1 water.
• colonization of bean plants with AM fungi increased
1. growth parameters,
2. yield parameters and
3. mineral nutrient concentrations and reduced disease
severity and disease incidence.
Fattah et al.,2010,Microbiological Researc
43. • Different physical and biochemical mechanisms play a role in
enhancement of plant resistance against Rhizoctonia solani, -
1. improved plant nutrition,
2. improved plant growth,
3. increase in cell wall thickening,
4. cytoplasmic granulation, and
5. accumulation of some antimicrobial substances (phenolic
compounds and defense related enzymes).
44. Fig 22-Pot experiment, effect of different treatments on the growth of the common
bean plants
45. Figure 23. Effect of mycorrhizal colonization with different treatments on defense related
enzyme activities in the root of common bean plants infected with Rhizoctonia root rot disease.
C, control; F, fungicide; P, pathogen.
46. • PAbs separately raised against F. solani, T. hamatum and G. mosseae
purified and packaged into serological formats (PTA-ELISA, DIBA,
western blot and immunofluorescence).
• Successful root colonization with G. mosseae confirmed by cellular
localization in mandarin root tissues (FITC labeled immunofluorescence
assay).
• Enhanced growth of the saplings in AMF inoculated plants.
• suppressed root rot of mandarin.
• Induction of major defense enzymes such as chitinase, β 1-3 glucanase
and peroxidase by treatment with AMF and T. hamatum.
Activation of defense Response of Mandarin plants Against Fusarium
Rot disease using Glomus mosseae and Trichoderma hamatum
Source-Allay and Chakraborty (2010),. J.Mycol.Pl.Pathol.
47. Antigen Source Pab chitinase Pab of beta 1,3-glucanase
A 405
ELISA
Colour intensity
Dot-Blot
A 405 ELISA Colour intensity
Dot-Blot
Control 0.034 Light pink 0.049 Light pink
Fusarium solani
inoculated
0.036 Light pink 0.052 Light pink
F.solani+G.mosseae
treated
0.520 Deep purplish 0.368 Dark pink
F.solani+T.hamatum
treated
0.768 Dark pink 0.445 Dark pink
F.solani+G.mosseae+T.h
amatum
0.982 Deep purplish 0.865 Deep purplish
Table 8-ELISA and Dot-blot values of reactions between Pabs of defense
Enzymes and enzyme extracts from treated mandarin plants-
48. 0
2
4
6
8
Rootrotindex
Effect of application of T.hamatum and G.mosseae
on root rot caused by F.solani on mandarin
15days
30days
45 days
Fig 24:Mandarin plants inoculated
With G.mosseae
and T.hamatum
49. Mycorrhizal symbioses have an important impact on
plant interactions with pathogens and insects.
Fungal stimulation of the plant immune system is solely
responsible for MIR.
MIR in aboveground tissues seems effective against
necrotrophic pathogens and generalist chewing insects
but not against biotrophs.
Mycorrhiza increases the susceptibility to viral disease.
CONCLUSION
50. MIR is partially determined by resistance-inducing
bacteria in the mycorrhizosphere .
Priming of plant immunity and jasmonate signaling plays
a major role in MIR.
In the future, mycorrhizosphere management must
become one of the viable and ecosystem friendly
solutions to managing plant diseases.